KEGG   ENZYME: 3.4.21.66
Entry
EC 3.4.21.66                Enzyme                                 

Name
thermitase;
thermophilic Streptomyces serine proteinase;
Thermoactinomyces vulgaris serine proteinase
Class
Hydrolases;
Acting on peptide bonds (peptidases);
Serine endopeptidases
Reaction(IUBMB)
Hydrolysis of proteins, including collagen
Comment
A peptidase of family S8 (subtilisin family) from Thermoactinomyces vulgaris containing a single Cys, near the active site His, and inhibited by p-mercuribenzoate. The N-terminal extension of the polypeptide chain relative to subtilisin contributes to Ca2+-binding and the high thermostability. The amino acid composition and properties of the thermostable enzyme from Streptomyces rectus var. proteolyticus (formerly included in EC 3.4.21.14) are closely similar [1,2].
History
EC 3.4.21.66 created 1992
Orthology
K08651  thermitase
Genes
PSU: Psesu_0863
LMB: C9I47_1968
LYT: DWG18_07540
LYJ: FKV23_06545 FKV23_10485
VAF: D1115_18240
VAS: GT360_16150
MDN: JT25_015795 JT25_015800
METL: U737_05595
MAH: MEALZ_2820
MBUR: EQU24_02085
MMAI: sS8_0778
TIG: THII_2403
NHL: Nhal_2234
AEH: Mlg_2387
HHA: Hhal_2368
HCH: HCH_03894
SVA: SVA_3720
NEU: NE1574
NET: Neut_0841
TBD: Tbd_0507
EBA: ebA1220
AZA: AZKH_4121
TCL: Tchl_1439
GUR: Gura_3316
DALK: DSCA_59550
BAN: BA_2380
BAR: GBAA_2380
BAT: BAS2216
BAI: BAA_2438
BANT: A16_24130
BANR: A16R_24400
BANS: BAPAT_2274
BANV: DJ46_1169(apr)
BCE: BC2316
BCA: BCE_2410
BCQ: BCQ_2306
BCX: BCA_2447
BNC: BCN_2301
BCF: bcf_11840
BCER: BCK_23010
BTL: BALH_2119
BTT: HD73_2601
BTHI: BTK_12915
BTM: MC28_1586
BTI: BTG_08260
BTW: BF38_3565(apr)
BMYC: DJ92_4728
BBEV: BBEV_2552
BHA: BH1491
BCL: ABC1758
OIH: OB2598
GTN: GTNG_1368
HLI: HLI_16300
PASA: BAOM_4719
PPM: PPSC2_16770(apr)
PPO: PPM_3387(apr)
PPOL: X809_32825
PPOY: RE92_20325
PMW: B2K_28265
PSAB: PSAB_17580
PRI: PRIO_5168
PSWU: SY83_14845
EFM: M7W_220
EDU: LIU_14385
CSQ: CSCA_2455
CTYK: CTK_C05950
HSD: SD1D_0291
SBH: SBI_05887
NML: Namu_3304
GOB: Gobs_2634
SESP: BN6_81520
ALO: CRK60299
SAQ: Sare_3266
CWO: Cwoe_0274
MPK: VL20_5283
RCA: Rcas_0223
STI: Sthe_2549
TTR: Tter_0357
DGE: Dgeo_2836
DFC: DFI_08065
MRI: Mal4_01060(prcA)
SACI: Sinac_0692
BXY: BXY_34550
SGN: SGRA_1885
LBY: Lbys_3027
KOS: KORDIASMS9_03542(prcA)
PLT: Plut_0364
PPH: Ppha_0453
IAL: IALB_0145
TTK: TST_0749
TLE: Tlet_2037
CABY: Cabys_1373
MEMA: MMAB1_2826
MEZ: Mtc_0702
RCI: RCIX1104
HME: HFX_0470(aprE)
 » show all
Reference
1  [PMID:4711613]
  Authors
Mizusawa K, Yoshida F.
  Title
Thermophilic Streptomyces alkaline proteinase. II. The role of a sulfhydryl group and the conformational stability.
  Journal
J Biol Chem 248:4417-23 (1973)
Reference
2  [PMID:4373436]
  Authors
Borgia P, Campbell LL.
  Title
Properties of two homologous alkaline proteases from Streptomyces rectus.
  Journal
J Bacteriol 120:1109-15 (1974)
DOI:10.1128/JB.120.3.1109-1115.1974
Reference
3  [PMID:7051706]
  Authors
Kleine R.
  Title
Properties of thermitase, a thermostable serine protease from Thermoactinomyces vulgaris.
  Journal
Acta Biol Med Ger 41:89-102 (1982)
Reference
4
  Authors
Meloun, B., Baudy, M., Kostka, V., Hausdorf, G., Frommel, C. and Hohne, W.E.
  Title
Complete primary structure of thermitase from Thermoactinomyces vulgaris and its structural features related to the subtilisin-type proteinases.
  Journal
FEBS Lett 183:195-200 (1985)
Reference
5  [PMID:2196375]
  Authors
Teplyakov AV, Kuranova IP, Harutyunyan EH, Vainshtein BK, Frommel C, Hohne WE, Wilson KS.
  Title
Crystal structure of thermitase at 1.4 A resolution.
  Journal
J Mol Biol 214:261-79 (1990)
DOI:10.1016/0022-2836(90)90160-N
Other DBs
ExplorEnz - The Enzyme Database: 3.4.21.66
IUBMB Enzyme Nomenclature: 3.4.21.66
ExPASy - ENZYME nomenclature database: 3.4.21.66
BRENDA, the Enzyme Database: 3.4.21.66
CAS: 69772-87-8

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