KEGG   ENZYME: 3.4.21.66
Entry
EC 3.4.21.66                Enzyme                                 
Name
thermitase;
thermophilic Streptomyces serine proteinase;
Thermoactinomyces vulgaris serine proteinase
Class
Hydrolases;
Acting on peptide bonds (peptidases);
Serine endopeptidases
Reaction(IUBMB)
Hydrolysis of proteins, including collagen
Comment
A peptidase of family S8 (subtilisin family) from Thermoactinomyces vulgaris containing a single Cys, near the active site His, and inhibited by p-mercuribenzoate. The N-terminal extension of the polypeptide chain relative to subtilisin contributes to Ca2+-binding and the high thermostability. The amino acid composition and properties of the thermostable enzyme from Streptomyces rectus var. proteolyticus (formerly included in EC 3.4.21.14) are closely similar [1,2].
History
EC 3.4.21.66 created 1992
Orthology
K08651  thermitase
Genes
PSUPsesu_0863
LMBC9I47_1968
LYTDWG18_07540
LYJFKV23_06545 FKV23_10485
VAFD1115_18240
VASGT360_16150
VZIG5S32_16325
MDNJT25_015795 JT25_015800
METLU737_05595
MAHMEALZ_2820
MBUREQU24_02085
MMAIsS8_0778
MEINmethR_P2970 methR_P2973
TSETHMIRHAS_23380
TIGTHII_2403
NHLNhal_2234
NWRE3U44_08505
TSYTHSYN_10940 THSYN_24380
AEHMlg_2387
HHAHhal_2368
TTITHITH_05010
HNAHneap_1707
HCHHCH_03894
HAHEENC22_12950
KAKKalk_10505
SVASVA_3720
GPBHDN1F_21970
BPYRABD05_14670
KIAG8A07_02025
MTIMDIR46_12445
NEUNE1574
NETNeut_0841
NIINit79A3_3269
NSTNstercoris_00357
NMUNmul_A1888 Nmul_A1891
NLCEBAPG3_007280 EBAPG3_007290
TBDTbd_0507
EBAebA1220
ABREpbN1_22810
APETToN1_20340
AZAAZKH_4121
AZIAzCIB_4097
AZRCJ010_02480
TCLTchl_1439
TAKTharo_3038
GMEGmet_0749 Gmet_0751 Gmet_1195
GURGura_3316
GSBGSUB_08230
DATHRM2_24400 HRM2_26300 HRM2_26400
DOVDSCO28_69020
DALKDSCA_59550
DLIdnl_11830 dnl_43200
CCXCOCOR_07741
DAXFDQ92_01500
ROMEI983_14095
SMUCJL100_003090
BBATBdt_0026 Bdt_1264 Bdt_2481 Bdt_2613
BBWBDW_00115 BDW_04405 BDW_09700
BBACEP01_09630 EP01_12170 EP01_16350
BDQCIK05_00120 CIK05_05405 CIK05_07450 CIK05_11540
BDCDOE51_00145 DOE51_05840 DOE51_12655 DOE51_13280 DOE51_15455
BDZDOM22_00120 DOM22_03680 DOM22_06415 DOM22_13550 DOM22_13955
BDKHW988_00120
BEXA11Q_1293 A11Q_1429 A11Q_1642 A11Q_1794 A11Q_1951 A11Q_26 A11Q_284 A11Q_946
BMXBMS_1029 BMS_1331 BMS_2097
HAXBALOs_0634 BALOs_1851 BALOs_2011 BALOs_2127
BSTOC0V70_06675 C0V70_10385 C0V70_11480 C0V70_12810
BANBA_2380
BARGBAA_2380
BATBAS2216
BAHBAMEG_2220
BAIBAA_2438
BAXH9401_2260
BANTA16_24130
BANRA16R_24400
BANSBAPAT_2274
BANHHYU01_11845
BANVDJ46_1169(apr)
BCEBC2316
BCABCE_2410
BCZBCE33L2139
BCRBCAH187_A2480
BCBBCB4264_A2345
BCUBCAH820_2398
BCGBCG9842_B2980
BCQBCQ_2306
BCXBCA_2447
BALBACI_c23250
BNCBCN_2301
BCFbcf_11840
BCERBCK_23010
BCEFBcrFT9_01888
BCYBcer98_2220
BTKBT9727_2155
BTLBALH_2119
BTBBMB171_C2085
BTTHD73_2601
BTHRYBT1520_12745
BTHIBTK_12915
BTCCT43_CH2277
BTFYBT020_12085
BTMMC28_1586
BTGBTB_c23960
BTIBTG_08260
BTNBTF1_09085
BTHTH175_328p188 H175_ch2313
BTHUYBT1518_01310 YBT1518_02065 YBT1518_12830 YBT1518_29045 YBT1518_34191
BTWBF38_3565(apr)
BTHYAQ980_18045
BTYBtoyo_4942
BMYCDJ92_4728
BBYCY96_10695
BWDCT694_12545
BTROFJR70_07810 FJR70_29730
BPACLMD38_14920
BPANNLJ82_11455
BMETBMMGA3_01145
BDAFSZ17_12775
BBADK7T73_04390
BACIB1NLA3E_12325
BACQDOE78_20730
BCOBcell_1945 Bcell_2754
BMHBMWSH_p10062
BFXBC359_08480
BHABH1491
BCLABC1758
BKWBkAM31D_14100
BPFBpOF4_14395
OIHOB2598
GTEGTCCBUS3UF5_17500
GTKGT3570_07330
GTMGT3921_11800
GLIGLN3_00980
BCALCWI35_02560
GTNGTNG_1368
GWCGWCH70_1928
GYCGYMC61_2354
GYAGYMC52_1482
GCTGC56T3_1996
GGHGHH_c15360
GJFM493_07845
GEAGARCT_01527
GSEGT50_00190
GSRGS3922_08975
GEJA0V43_04945
PTBDER53_14090
PCALBV455_00207 BV455_02978
PARGPspKH34_32350
ACAIISX45_07555 ISX45_14330
HMNHM131_01595 HM131_06185
HLIHLI_16300
VIMGWK91_08240
FPNABE65_006275 ABE65_018390 ABE65_019505
FARABE41_007270 ABE41_018575
PASABAOM_4719
PPSRI6J18_15970
AQTFN924_16280
PRDF7984_12960
POFGS400_19585
NDTL1999_03125
MDGK8L98_21215
SEDDERJ70_06025
RMFD5E69_11015
BBEVBBEV_2552
EACEKKI46_06685
BBEBBR47_21070
BLRBRLA_c014640 BRLA_c033740
BFMBP422_05720
BAGRBA6348_18550
BRWGOP56_00245 GOP56_08980
BCHSJNE38_11120 JNE38_20120
PJDPjdr2_2248
PPMPPSC2_16770(apr)
PPOPPM_3387(apr)
PPOLX809_32825
PPQPPSQR21_033820
PPOYRE92_20325
PMSKNP414_04106
PMQPM3016_5549
PMWB2K_28265
PTAHPL003_25350
PLVERIC2_c34730
PSABPSAB_17580
PDUPDUR_20015
PBDPBOR_27385
PGMPGRAT_24765
PODPODO_23515
PAENP40081_29070
PAEFR50345_23265
PAEQR50912_25640
PSTEPSTEL_19650
PAEAR70723_24770
PAEER70331_23625
PAEHH70357_26790
PAEJH70737_23325
PBJVN24_19425
PRIPRIO_5168
PSONJI735_00880
PNPIJ22_09440
PXLBS614_21700 BS614_24655
PYGAWM70_11470
PSWUSY83_14845
PDHB9T62_24005
PAIHASL14_21490
PVOPVOR_11119
PPSCEHS13_13040
PALBEJC50_13115
PCHIPC41400_10790 PC41400_23050
PBKBack11_26060
PPRTET464_07600
PBACHUB98_04295 HUB98_08345
PRZGZH47_05850
PLYCGXP70_10410
PSOPKP014_24170
PTRIKDC22_25080
PTJJRJ22_22005
PALRHGI30_01955
TUMCBW65_02315 CBW65_02320 CBW65_02325 CBW65_02330 CBW65_02335 CBW65_02340 CBW65_16355 CBW65_16810 CBW65_18875
TABCIG75_04325 CIG75_04330 CIG75_04335 CIG75_12615
EFFskT53_00730
PRTAUC31_15585
PPLABBI15_07000
PLXCW734_13040
PMATBBI11_12840
PGQFK545_02760
LFBC1X05_05205 C1X05_05210 C1X05_05215 C1X05_14610 C1X05_15305
TVUAB849_000840 AB849_005595 AB849_009225
KPULGXN76_05715 GXN76_13910
KEBGXN75_04180
PABSJIR001_03610 JIR001_09320 JIR001_09330 JIR001_29970
EFMM7W_220
EDULIU_14385
CAWQ783_12165
CLBClo1100_3059
CSQCSCA_2455
CTYKCTK_C05950
CFMBJL90_00400
CDRKB9W14_07405
CRSFQB35_04635
CTXClo1313_0689
HSDSD1D_0291
ACELacsn021_18200
TEMJW646_19465
AWOAwo_c03440 Awo_c07490 Awo_c18100 Awo_c23480 Awo_c25570
AMIJEQM06_06680
ABUTAmi103574_09395
NCDACONDI_00280
AFTBBF96_11540
ERMEYR00_14890
MDRMDOR_17770
CCYCSCMU_35160
SASTCD934_05890
SXNIAG42_18535
SDECL3078_06615
ARTArth_1050 Arth_1052
ARQBWQ92_04810 BWQ92_04815
ACRYAC20117_11930
ARTHC3B78_05090
ARTYAHiyo4_11920
APNAsphe3_10920
PSULAU252_05035
PSNINIBR502771_20255
PSEYGU243_21495 GU243_21615
JTEASJ30_11390
NPIG7071_00665
AEBC6I20_12490
GOBGobs_2634
SACGFDZ84_09845
SVISvir_22270
SESPBN6_81520
KPHYAOZ06_50775
ACTAC1701_12510
ALOCRK60299
SACEGIY23_21180
STPStrop_3040
SAQSare_3266
AMSAMIS_64790 AMIS_6950
ACTNL083_1023 L083_1024 L083_4474
AFSAFR_04970 AFR_04975 AFR_16425 AFR_17405
AIHAiant_45890
PRYPrubr_26450
RXYRxyl_1295 Rxyl_2188
RRDRradSPS_0222 RradSPS_1259 RradSPS_1976 RradSPS_3123
RUBGBA63_09485 GBA63_20955
RMARGBA65_03350 GBA65_09385 GBA65_18480 GBA65_18500 GBA65_20250
CWOCwoe_0274
PARNNBH00_02820 NBH00_07970 NBH00_08600
ERZER308_20505
EUZDVS28_a1581
PMFP9303_10331
MPKVL20_5283
CWACwatDRAFT_2267
CYCPCC7424_0552
ANNEH233_18225
TOQHCG51_30890
NCNBZZ01_15620
RRSRoseRS_0664 RoseRS_0678
RCARcas_0223
CHLChy400_3661
HAUHaur_1188 Haur_2156 Haur_2203 Haur_3265 Haur_3542 Haur_5185
TROtrd_1587 trd_A0692
STISthe_2549
CAPCLDAP_00780 CLDAP_28370
THUGKNN16_09830 KNN16_14630
TTRTter_0357
DDRDeide_01760 Deide_01770 Deide_1p00720 Deide_1p00730 Deide_1p00930 Deide_1p00940
DMRDeima_1867 Deima_2872
DPDDeipe_0144 Deipe_2811 Deipe_4220
DFCDFI_08065
DEINDAAJ005_17985
FGIOP10G_3378
NPYNPRO_00810 NPRO_12070 NPRO_22330
SOAG3M56_001700
FMRFuma_02040
MRIMal4_01060(prcA)
GESVT84_35395
SACISinac_0692
PBORBSF38_02588
BROCIPI25_13270 IPI25_13275
JETL3J17_02630
HBSIPV69_03235
TAERGT409_03895
PFERIRI77_28795
CPORBED41_13720
BXYBXY_34550
BOABovatus_04574
BCAEA4V03_03010
PEOAS203_03020
APUTL6V35_03200 L6V35_06455
RBARAWN76_000220 AWN76_013495 AWN76_013630
CFILMYF79_27490
NSONIASO_01750
AGIFSB73_01150
PGOFSB84_15910
HHYHalhy_2718 Halhy_4825 Halhy_5883
SGNSGRA_1885
PHEPhep_1319 Phep_3748
PCMAY601_2980
MGOSDIU38_011040
MDAIPZ59_04265
SPIBG8759_27140
LBYLbys_3027
FIBA6C57_19635
FLIFleli_3875
HQIH9L05_09390
FPFDCC35_08005
FLLEI427_08900
CHKD4L85_19290
FKIFK004_03205 FK004_03230
FCRHYN56_21970
FBCFB2170_13978
SALTAO058_16605
KOSKORDIASMS9_03542(prcA)
AQDD1816_04515 D1816_15225
ORHOrnrh_2152
ORIQ785_10880
ESTEHNV03_04620
PLTPlut_0364
PPHPpha_0453
IALIALB_0145
CPRVCYPRO_2908 CYPRO_2920
TTKTST_0749
TLETlet_2037
PHYAJ81_03835
DDFDEFDS_0796
BIHBIP78_0310 BIP78_0819
MIBUY43_C0001G0802
METTCIT01_05975
MHUMhun_0018 Mhun_0486 Mhun_0648 Mhun_1456 Mhun_1944 Mhun_2053 Mhun_2479
MRTJKHC33_00045 KHC33_03980 KHC33_07500 KHC33_10125 KHC33_11650 KHC33_11965 KHC33_14100 KHC33_14165 KHC33_14700
MBGBN140_2139
MEMAMMAB1_2826
MEZMtc_0702
RCIRCIX1104
HMEHFX_0470(aprE)
PSYTDSAG12_01024
 » show all
Reference
1  [PMID:4711613]
  Authors
Mizusawa K, Yoshida F.
  Title
Thermophilic Streptomyces alkaline proteinase. II. The role of a sulfhydryl group and the conformational stability.
  Journal
J Biol Chem 248:4417-23 (1973)
Reference
2  [PMID:4373436]
  Authors
Borgia P, Campbell LL.
  Title
Properties of two homologous alkaline proteases from Streptomyces rectus.
  Journal
J Bacteriol 120:1109-15 (1974)
DOI:10.1128/JB.120.3.1109-1115.1974
Reference
3  [PMID:7051706]
  Authors
Kleine R.
  Title
Properties of thermitase, a thermostable serine protease from Thermoactinomyces vulgaris.
  Journal
Acta Biol Med Ger 41:89-102 (1982)
Reference
4
  Authors
Meloun, B., Baudy, M., Kostka, V., Hausdorf, G., Frommel, C. and Hohne, W.E.
  Title
Complete primary structure of thermitase from Thermoactinomyces vulgaris and its structural features related to the subtilisin-type proteinases.
  Journal
FEBS Lett 183:195-200 (1985)
Reference
5  [PMID:2196375]
  Authors
Teplyakov AV, Kuranova IP, Harutyunyan EH, Vainshtein BK, Frommel C, Hohne WE, Wilson KS.
  Title
Crystal structure of thermitase at 1.4 A resolution.
  Journal
J Mol Biol 214:261-79 (1990)
DOI:10.1016/0022-2836(90)90160-N
Other DBs
ExplorEnz - The Enzyme Database: 3.4.21.66
IUBMB Enzyme Nomenclature: 3.4.21.66
ExPASy - ENZYME nomenclature database: 3.4.21.66
BRENDA, the Enzyme Database: 3.4.21.66
CAS: 69772-87-8

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