Entry
Name
thermitase;
thermophilic Streptomyces serine proteinase;
Thermoactinomyces vulgaris serine proteinase
Class
Hydrolases;
Acting on peptide bonds (peptidases);
Serine endopeptidases
BRITE hierarchy
Reaction(IUBMB)
Hydrolysis of proteins, including collagen
Comment
A peptidase of family S8 (subtilisin family) from Thermoactinomyces vulgaris containing a single Cys, near the active site His, and inhibited by p-mercuribenzoate. The N-terminal extension of the polypeptide chain relative to subtilisin contributes to Ca2+-binding and the high thermostability. The amino acid composition and properties of the thermostable enzyme from Streptomyces rectus var. proteolyticus (formerly included in EC
3.4.21.14 ) are closely similar [1,2].
History
EC 3.4.21.66 created 1992
Orthology
Genes
LYJ : FKV23_06545 FKV23_10485
MDN : JT25_015795 JT25_015800
MEIN : methR_P2970 methR_P2973
TSY : THSYN_10940 THSYN_24380
NMU : Nmul_A1888 Nmul_A1891
NLC : EBAPG3_007280 EBAPG3_007290
GME : Gmet_0749 Gmet_0751 Gmet_1195
DAT : HRM2_24400 HRM2_26300 HRM2_26400
BBAT : Bdt_0026 Bdt_1264 Bdt_2481 Bdt_2613
BBW : BDW_00115 BDW_04405 BDW_09700
BBAC : EP01_09630 EP01_12170 EP01_16350
BDQ : CIK05_00120 CIK05_05405 CIK05_07450 CIK05_11540
BDC : DOE51_00145 DOE51_05840 DOE51_12655 DOE51_13280 DOE51_15455
BDZ : DOM22_00120 DOM22_03680 DOM22_06415 DOM22_13550 DOM22_13955
BEX : A11Q_1293 A11Q_1429 A11Q_1642 A11Q_1794 A11Q_1951 A11Q_26 A11Q_284 A11Q_946
BMX : BMS_1029 BMS_1331 BMS_2097
HAX : BALOs_0634 BALOs_1851 BALOs_2011 BALOs_2127
BSTO : C0V70_06675 C0V70_10385 C0V70_11480 C0V70_12810
BTHT : H175_328p188 H175_ch2313
BTHU : YBT1518_01310 YBT1518_02065 YBT1518_12830 YBT1518_29045 YBT1518_34191
BTRO : FJR70_07810 FJR70_29730
BCO : Bcell_1945 Bcell_2754
PCAL : BV455_00207 BV455_02978
ACAI : ISX45_07555 ISX45_14330
HMN : HM131_01595 HM131_06185
FPN : ABE65_006275 ABE65_018390 ABE65_019505
FAR : ABE41_007270 ABE41_018575
BLR : BRLA_c014640 BRLA_c033740
BRW : GOP56_00245 GOP56_08980
BCHS : JNE38_11120 JNE38_20120
PXL : BS614_21700 BS614_24655
PCHI : PC41400_10790 PC41400_23050
PBAC : HUB98_04295 HUB98_08345
TUM : CBW65_02315 CBW65_02320 CBW65_02325 CBW65_02330 CBW65_02335 CBW65_02340 CBW65_16355 CBW65_16810 CBW65_18875
TAB : CIG75_04325 CIG75_04330 CIG75_04335 CIG75_12615
LFB : C1X05_05205 C1X05_05210 C1X05_05215 C1X05_14610 C1X05_15305
TVU : AB849_000840 AB849_005595 AB849_009225
KPUL : GXN76_05715 GXN76_13910
PABS : JIR001_03610 JIR001_09320 JIR001_09330 JIR001_29970
AWO : Awo_c03440 Awo_c07490 Awo_c18100 Awo_c23480 Awo_c25570
ARQ : BWQ92_04810 BWQ92_04815
PSEY : GU243_21495 GU243_21615
AMS : AMIS_64790 AMIS_6950
ACTN : L083_1023 L083_1024 L083_4474
AFS : AFR_04970 AFR_04975 AFR_16425 AFR_17405
RRD : RradSPS_0222 RradSPS_1259 RradSPS_1976 RradSPS_3123
RUB : GBA63_09485 GBA63_20955
RMAR : GBA65_03350 GBA65_09385 GBA65_18480 GBA65_18500 GBA65_20250
PARN : NBH00_02820 NBH00_07970 NBH00_08600
RRS : RoseRS_0664 RoseRS_0678
HAU : Haur_1188 Haur_2156 Haur_2203 Haur_3265 Haur_3542 Haur_5185
CAP : CLDAP_00780 CLDAP_28370
THUG : KNN16_09830 KNN16_14630
DDR : Deide_01760 Deide_01770 Deide_1p00720 Deide_1p00730 Deide_1p00930 Deide_1p00940
DMR : Deima_1867 Deima_2872
DPD : Deipe_0144 Deipe_2811 Deipe_4220
NPY : NPRO_00810 NPRO_12070 NPRO_22330
BROC : IPI25_13270 IPI25_13275
APUT : L6V35_03200 L6V35_06455
RBAR : AWN76_000220 AWN76_013495 AWN76_013630
HHY : Halhy_2718 Halhy_4825 Halhy_5883
FKI : FK004_03205 FK004_03230
KOS : KORDIASMS9_03542(prcA)
AQD : D1816_04515 D1816_15225
CPRV : CYPRO_2908 CYPRO_2920
BIH : BIP78_0310 BIP78_0819
MHU : Mhun_0018 Mhun_0486 Mhun_0648 Mhun_1456 Mhun_1944 Mhun_2053 Mhun_2479
MRTJ : KHC33_00045 KHC33_03980 KHC33_07500 KHC33_10125 KHC33_11650 KHC33_11965 KHC33_14100 KHC33_14165 KHC33_14700
» show all
Taxonomy
Reference
Authors
Mizusawa K, Yoshida F.
Title
Thermophilic Streptomyces alkaline proteinase. II. The role of a sulfhydryl group and the conformational stability.
Journal
J Biol Chem 248:4417-23 (1973)
Reference
Authors
Borgia P, Campbell LL.
Title
Properties of two homologous alkaline proteases from Streptomyces rectus.
Journal
Reference
Authors
Kleine R.
Title
Properties of thermitase, a thermostable serine protease from Thermoactinomyces vulgaris.
Journal
Acta Biol Med Ger 41:89-102 (1982)
Reference
4
Authors
Meloun, B., Baudy, M., Kostka, V., Hausdorf, G., Frommel, C. and Hohne, W.E.
Title
Complete primary structure of thermitase from Thermoactinomyces vulgaris and its structural features related to the subtilisin-type proteinases.
Journal
FEBS Lett 183:195-200 (1985)
Reference
Authors
Teplyakov AV, Kuranova IP, Harutyunyan EH, Vainshtein BK, Frommel C, Hohne WE, Wilson KS.
Title
Crystal structure of thermitase at 1.4 A resolution.
Journal
Other DBs
ExPASy - ENZYME nomenclature database: 3.4.21.66