KEGG ENZYME: 3.4.21.66

ENZYME: 3.4.21.66

Entry

EC 3.4.21.66 Enzyme

Name

thermitase;
thermophilic Streptomyces serine proteinase;
Thermoactinomyces vulgaris serine proteinase

Class

Hydrolases;
Acting on peptide bonds (peptidases);
Serine endopeptidases

Reaction(IUBMB)

Hydrolysis of proteins, including collagen

Comment

A peptidase of family S8 (subtilisin family) from Thermoactinomyces vulgaris containing a single Cys, near the active site His, and inhibited by p-mercuribenzoate. The N-terminal extension of the polypeptide chain relative to subtilisin contributes to Ca2+-binding and the high thermostability. The amino acid composition and properties of the thermostable enzyme from Streptomyces rectus var. proteolyticus (formerly included in EC 3.4.21.14) are closely similar [1,2].

History

EC 3.4.21.66 created 1992

Orthology

K08651

thermitase

Genes

PSU:	Psesu_0863

LMB:	C9I47_1968

LYT:	DWG18_07540

LYJ:	FKV23_06545 FKV23_10485

VAF:	D1115_18240

VAS:	GT360_16150

MDN:	JT25_015795 JT25_015800

METL:

U737_05595

MAH:	MEALZ_2820

MBUR:

EQU24_02085

MMAI:

sS8_0778

MEIN:

methR_P2970 methR_P2973

TSE:	THMIRHAS_23380

TIG:

THII_2403

NHL:

Nhal_2234

NWR:	E3U44_08505

TSY:	THSYN_10940 THSYN_24380

AEH:

Mlg_2387

HHA:

Hhal_2368

TTI:	THITH_05010

HNA:	Hneap_1707

HCH:

HCH_03894

HAHE:

ENC22_12950

KAK:	Kalk_10505

SVA:

SVA_3720

GPB:	HDN1F_21970

BPYR:

MTIM:

NEU:

NET:

NII:	Nit79A3_3269

NST:	Nstercoris_00357

NMU:	Nmul_A1888 Nmul_A1891

NLC:	EBAPG3_007280 EBAPG3_007290

TBD:

Tbd_0507

EBA:

ebA1220

AZA:

AZKH_4121

AZI:	AzCIB_4097

AZR:	CJ010_02480

TCL:

Tchl_1439

TAK:	Tharo_3038

GME:	Gmet_0749 Gmet_0751 Gmet_1195

GUR:

Gura_3316

GSB:	GSUB_08230

DAT:	HRM2_24400 HRM2_26300 HRM2_26400

DOV:	DSCO28_69020

DALK:

DSCA_59550

CCX:	COCOR_07741

DAX:	FDQ92_01500

ROM:	EI983_14095

BBAT:

Bdt_0026 Bdt_1264 Bdt_2481 Bdt_2613

BBW:	BDW_00115 BDW_04405 BDW_09700

BBAC:

EP01_09630 EP01_12170 EP01_16350

BEX:	A11Q_1293 A11Q_1429 A11Q_1642 A11Q_1794 A11Q_1951 A11Q_26 A11Q_284 A11Q_946

BDQ:	CIK05_00120 CIK05_05405 CIK05_07450 CIK05_11540

BDC:	DOE51_00145 DOE51_05840 DOE51_12655 DOE51_13280 DOE51_15455

BDZ:	DOM22_00120 DOM22_03680 DOM22_06415 DOM22_13550 DOM22_13955

BMX:	BMS_1029 BMS_1331 BMS_2097

HAX:	BALOs_0634 BALOs_1851 BALOs_2011 BALOs_2127

BSTO:

C0V70_06675 C0V70_10385 C0V70_11480 C0V70_12810

BAN:

BA_2380

BAR:

GBAA_2380

BAT:

BAS2216

BAH:	BAMEG_2220

BAI:

BAA_2438

BAX:	H9401_2260

BANT:

BANR:

BANS:

BANH:

BANV:

DJ46_1169(apr)

BCE:

BC2316

BCA:

BCE_2410

BCZ:	BCE33L2139

BCR:	BCAH187_A2480

BCB:	BCB4264_A2345

BCU:	BCAH820_2398

BCG:	BCG9842_B2980

BCQ:

BCQ_2306

BCX:

BCA_2447

BAL:	BACI_c23250

BNC:

BCF:

BCER:

BCEF:

BCY:	Bcer98_2220

BTK:	BT9727_2155

BTL:

BALH_2119

BTB:	BMB171_C2085

BTT:

HD73_2601

BTHR:

YBT1520_12745

BTHI:

BTK_12915

BTC:	CT43_CH2277

BTF:	YBT020_12085

BTM:

MC28_1586

BTG:	BTB_c23960

BTI:

BTG_08260

BTN:	BTF1_09085

BTHT:

H175_328p188 H175_ch2313

BTHU:

YBT1518_01310 YBT1518_02065 YBT1518_12830 YBT1518_29045 YBT1518_34191

BTW:	BF38_3565(apr)

BTHY:

AQ980_18045

BTY:	Btoyo_4942

BMYC:

DJ92_4728

BBY:	CY96_10695

BWD:	CT694_12545

BTRO:

FJR70_07810 FJR70_29730

BCO:	Bcell_1945 Bcell_2754

BACI:

BMET:

BBEV:

BACQ:

BDA:	FSZ17_12775

BMH:	BMWSH_p10062

BFX:	BC359_08480

BHA:

BH1491

BCL:

ABC1758

BPF:	BpOF4_14395

BKW:	BkAM31D_14100

OIH:

OB2598

GTE:	GTCCBUS3UF5_17500

GTK:	GT3570_07330

GTM:	GT3921_11800

GLI:	GLN3_00980

GTN:

GTNG_1368

GWC:	GWCH70_1928

GYC:	GYMC61_2354

GYA:	GYMC52_1482

GCT:	GC56T3_1996

GGH:	GHH_c15360

GJF:	M493_07845

GEA:	GARCT_01527

GSE:	GT50_00190

GSR:	GS3922_08975

GEJ:	A0V43_04945

PTB:	DER53_14090

ACAI:

ISX45_07555 ISX45_14330

HMN:	HM131_01595 HM131_06185

HLI:

HLI_16300

VIM:	GWK91_08240

FPN:	ABE65_006275 ABE65_018390 ABE65_019505

FAR:	ABE41_007270 ABE41_018575

PASA:

BAOM_4719

AQT:	FN924_16280

PRD:	F7984_12960

POF:	GS400_19585

BBE:	BBR47_21070

BLR:	BRLA_c014640 BRLA_c033740

BFM:	BP422_05720

BAGR:

BA6348_18550

BRW:	GOP56_00245 GOP56_08980

PJD:	Pjdr2_2248

PPM:	PPSC2_16770(apr)

PPO:	PPM_3387(apr)

PPOL:

X809_32825

PPQ:	PPSQR21_033820

PPOY:

RE92_20325

PMS:	KNP414_04106

PMQ:	PM3016_5549

PMW:

B2K_28265

PTA:	HPL003_25350

PLV:	ERIC2_c34730

PSAB:

PSAB_17580

PDU:	PDUR_20015

PBD:	PBOR_27385

PGM:	PGRAT_24765

POD:	PODO_23515

PAEN:

PAEF:

PAEQ:

PSTE:

PAEA:

PAEE:

PAEH:

PAEJ:

PBJ:	VN24_19425

PRI:

PRIO_5168

PNP:	IJ22_09440

PXL:	BS614_21700 BS614_24655

PYG:	AWM70_11470

PSWU:

SY83_14845

PDH:	B9T62_24005

PAIH:

ASL14_21490

PVO:	PVOR_11119

PPSC:

EHS13_13040

PALB:

EJC50_13115

PCHI:

PC41400_10790 PC41400_23050

PBK:	Back11_26060

PPRT:

ET464_07600

PBAC:

HUB98_04295 HUB98_08345

PRZ:	GZH47_05850

PLYC:

GXP70_10410

TUM:	CBW65_02315 CBW65_02320 CBW65_02325 CBW65_02330 CBW65_02335 CBW65_02340 CBW65_16355 CBW65_16810 CBW65_18875

TAB:	CIG75_04325 CIG75_04330 CIG75_04335 CIG75_12615

EFF:	skT53_00730

PRT:	AUC31_15585

PPLA:

BBI15_07000

PLX:	CW734_13040

PMAT:

BBI11_12840

PGQ:	FK545_02760

LFB:	C1X05_05205 C1X05_05210 C1X05_05215 C1X05_14610 C1X05_15305

TVU:	AB849_000840 AB849_005595 AB849_009225

KPUL:

GXN76_05715 GXN76_13910

KEB:	GXN75_04180

EFM:

M7W_220

EDU:

LIU_14385

CAW:	Q783_12165

CLB:	Clo1100_3059

CSQ:

CSCA_2455

CTYK:

CTK_C05950

CFM:	BJL90_00400

CDRK:

B9W14_07405

CRS:	FQB35_04635

CTX:	Clo1313_0689

HSD:

SD1D_0291

ACEL:

acsn021_18200

AWO:	Awo_c03440 Awo_c07490 Awo_c18100 Awo_c23480 Awo_c25570

AMIJ:

EQM06_06680

ABUT:

Ami103574_09395

AFT:	BBF96_11540

ERM:	EYR00_14890

MDR:	MDOR_17770

SBH:

SBI_05887

SAST:

CD934_05890

ART:	Arth_1050 Arth_1052

ARQ:	BWQ92_04810 BWQ92_04815

ACRY:

AC20117_11930

ARTH:

C3B78_05090

APN:	Asphe3_10920

PSUL:

AU252_05035

PSNI:

NIBR502771_20255

PSEY:

GU243_21495 GU243_21615

JTE:	ASJ30_11390

AEB:	C6I20_12490

NOA:	BKM31_58330

NML:

GOB:

SACE:

SACG:

SVI:	Svir_22270

SESP:

KPHY:

ACTA:

ALO:

STP:	Strop_3040

SAQ:

Sare_3266

AMS:	AMIS_64790 AMIS_6950

ACTN:

L083_1023 L083_1024 L083_4474

AFS:	AFR_04970 AFR_04975 AFR_16425 AFR_17405

RXY:	Rxyl_1295 Rxyl_2188

RRD:	RradSPS_0222 RradSPS_1259 RradSPS_1976 RradSPS_3123

RUB:	GBA63_09485 GBA63_20955

CWO:

Cwoe_0274

ERZ:	ER308_20505

EUZ:	DVS28_a1581

PMF:	P9303_10331

MPK:

VL20_5283

CWA:	CwatDRAFT_2267

CYC:	PCC7424_0552

ANN:	EH233_18225

TOQ:	HCG51_30890

NCN:	BZZ01_15620

RRS:	RoseRS_0664 RoseRS_0678

RCA:

Rcas_0223

CHL:	Chy400_3661

HAU:	Haur_1188 Haur_2156 Haur_2203 Haur_3265 Haur_3542 Haur_5185

TRO:	trd_1587 trd_A0692

STI:

Sthe_2549

CAP:	CLDAP_00780 CLDAP_28370

TTR:

Tter_0357

DGE:

Dgeo_2836

DDR:	Deide_01760 Deide_01770 Deide_1p00720 Deide_1p00730 Deide_1p00930 Deide_1p00940

DMR:	Deima_1867 Deima_2872 Deima_3125

DPD:	Deipe_0144 Deipe_2811 Deipe_4220

DFC:

DFI_08065

DEIN:

DAAJ005_17985

FGI:	OP10G_3378

FMR:	Fuma_02040

MRI:	Mal4_01060(prcA)

GES:	VT84_35395

SACI:

Sinac_0692

PBOR:

BSF38_02588

BROC:

IPI25_13270 IPI25_13275

HBS:	IPV69_03235

PFER:

IRI77_28795

CPOR:

BED41_13720

BXY:

BXY_34550

BOA:	Bovatus_04574

BCAE:

A4V03_03010

PEO:	AS203_03020

RBAR:

AWN76_000220 AWN76_013495 AWN76_013630

NSO:	NIASO_01750

AGI:	FSB73_01150

PGO:	FSB84_15910

HHY:	Halhy_2718 Halhy_4825 Halhy_5883

SGN:

SGRA_1885

PHE:	Phep_1319 Phep_3748

PCM:	AY601_2980

MGOS:

DIU38_011040

SPIB:

G8759_27140

LBY:

Lbys_3027

FIB:	A6C57_19635

FLI:	Fleli_3875

HQI:	H9L05_09390

FPF:	DCC35_08005

FLL:	EI427_08900

CHK:	D4L85_19290

FKI:	FK004_03205 FK004_03230

FPAL:

HYN49_14005

FCR:	HYN56_21970

FBC:	FB2170_13978

SALT:

AO058_16605

KOS:	KORDIASMS9_03542(prcA)

AQD:	D1816_04515 D1816_15225

ORH:	Ornrh_2152

ORI:	Q785_10880

ESTE:

APIB:

PLT:

PPH:

IAL:

CPRV:

CYPRO_2908 CYPRO_2920

TTK:

TST_0749

TLE:

Tlet_2037

PHY:	AJ81_03835

DDF:	DEFDS_0796

CABY:

Cabys_1373

BIH:	BIP78_0310 BIP78_0819

MIB:	UY43_C0001G0802

METT:

CIT01_05975

MHU:	Mhun_0018 Mhun_0486 Mhun_0648 Mhun_1456 Mhun_1944 Mhun_2053 Mhun_2479

MBG:	BN140_2139

MEMA:

MMAB1_2826

MEZ:

Mtc_0702

RCI:

RCIX1104

HME:	HFX_0470(aprE)

PSYT:

DSAG12_01024

» show all

Reference

1 [PMID:4711613]

Authors

Mizusawa K, Yoshida F.

Title

Thermophilic Streptomyces alkaline proteinase. II. The role of a sulfhydryl group and the conformational stability.

Journal

J Biol Chem 248:4417-23 (1973)

Reference

2 [PMID:4373436]

Authors

Borgia P, Campbell LL.

Title

Properties of two homologous alkaline proteases from Streptomyces rectus.

Journal

J Bacteriol 120:1109-15 (1974)
DOI:10.1128/JB.120.3.1109-1115.1974

Reference

3 [PMID:7051706]

Authors

Kleine R.

Title

Properties of thermitase, a thermostable serine protease from Thermoactinomyces vulgaris.

Journal

Acta Biol Med Ger 41:89-102 (1982)

Reference

Authors

Meloun, B., Baudy, M., Kostka, V., Hausdorf, G., Frommel, C. and Hohne, W.E.

Title

Complete primary structure of thermitase from Thermoactinomyces vulgaris and its structural features related to the subtilisin-type proteinases.

Journal

FEBS Lett 183:195-200 (1985)

Reference

5 [PMID:2196375]

Authors

Teplyakov AV, Kuranova IP, Harutyunyan EH, Vainshtein BK, Frommel C, Hohne WE, Wilson KS.

Title

Crystal structure of thermitase at 1.4 A resolution.

Journal

J Mol Biol 214:261-79 (1990)
DOI:10.1016/0022-2836(90)90160-N

Other DBs

ExplorEnz - The Enzyme Database:

3.4.21.66

IUBMB Enzyme Nomenclature:

3.4.21.66

ExPASy - ENZYME nomenclature database:

3.4.21.66

BRENDA, the Enzyme Database:

3.4.21.66

CAS:	69772-87-8

DBGET integrated database retrieval system