Entry
Name
bleomycin hydrolase;
aminopeptidase C (Lactococcus lactis) [4]
Class
Hydrolases;
Acting on peptide bonds (peptidases);
Cysteine endopeptidases
BRITE hierarchy
Reaction(IUBMB)
Inactivates bleomycin B2 (a cytotoxic glycometallopeptide) by hydrolysis of a carboxyamide bond of beta-aminoalanine, but also shows general aminopeptidase activity. The specificity varies somewhat with source, but amino acid arylamides of Met, Leu and Ala are preferred [1]
Comment
The molecule is a homohexamer in which the monomers have a papain-like tertiary structure (in peptidase family C1). The active sites are on the walls of a central channel through the molecule, and access of substrate molecules to them is obstructed by this and by the C-terminus of each polypeptide chain [3]. Bleomycin can scarcely be the natural substrate, and there are reports of limited endopeptidase activity. Known from bacteria as well as eukaryotic organisms. Hydrolase H from chicken muscle has many similarities to bleomycin hydrolase, but hydrolyses Ph-CO-Arg-2-naphthylamine as well as aminopeptidase substrates [2].
History
EC 3.4.22.40 created 2000
Orthology
Genes
PLEU : 114691459(Blmh) 114695678
ODA : 120862242 120866246(BLMH)
VPC : 102534062 102535868(BLMH)
PDIC : 114503106(BLMH) 114504888
PHAS : 123817713(BLMH) 123830110
PSS : 102462350 102462832(BLMH)
CTIG : 120296833 120297102
HCG : 128335941(BLMH) 128335963
XLA : 108707946(blmh.L) 734349(blmh.S)
SANH : 107669757 107685090
CAUA : 113058535 113114848
CGIB : 127973004 128029519
MASI : 127422393 127429479
SASA : 106580795(blmh) 106612652
STRU : 115163112(blmh) 115206061
SALP : 111949477 111960488
CCLU : 121563418 121567473
PSPA : 121308609(blmh) 121327498
ARUT : 117429588 117430219
DME : Dmel_CG13423(CG13423) Dmel_CG1440(CG1440)
DSI : Dsimw501_GD11522(Dsim_GD11522) Dsimw501_GD16898(Dsim_GD16898)
DYA : Dyak_GE12121 Dyak_GE15781
DMN : 108160582 108162426 117185785
DMO : Dmoj_GI15765 Dmoj_GI20946
CPII : 120430876 120431718
BCOO : 119076407 119083405
CGIG : 122396095 122396176
AGB : 108905919 108915338 111691288
BANY : 112057167 112057851
RMD : 113558079 113558080 113560407
PPOI : 119093832 119093903 119112150
RSAN : 119376983 119389685
NCS : NCAS_0G03590(NCAS0G03590)
NDI : NDAI_0F03900(NDAI0F03900)
TPF : TPHA_0P01800(TPHA0P01800)
TBL : TBLA_0B00120(TBLA0B00120)
TDL : TDEL_0E00400(TDEL0E00400)
KAF : KAFR_0A08050(KAFR0A08050)
KNG : KNAG_0F00760(KNAG0F00760)
CAL : CAALFM_CR04480CA(LAP3)
YLI : YALI0F05940g YALI0F17974g
CLU : CLUG_00029 CLUG_01084
CLUS : A9F13_09g01375 A9F13_19g00759
CAUR : CJI96_0000793 CJI96_0003227
PKZ : C5L36_0C08730 C5L36_0E01270
BNN : FOA43_002361 FOA43_004609
BBRX : BRETT_001803 BRETT_005152
OPA : HPODL_03714 HPODL_03865
NTE : NEUTE1DRAFT125878(NEUTE1DRAFT_125878)
BFU : BCIN_08g01120(Bclap3)
ANG : ANI_1_216014(An01g01720)
DSQ : DICSQDRAFT_143946 DICSQDRAFT_166325
ADL : AURDEDRAFT_129391 AURDEDRAFT_178787
FME : FOMMEDRAFT_108627 FOMMEDRAFT_85586
GTR : GLOTRDRAFT_45763 GLOTRDRAFT_58931
LBC : LACBIDRAFT_183606 LACBIDRAFT_312916
ABP : AGABI1DRAFT116802(AGABI1DRAFT_116802)
ABV : AGABI2DRAFT192278(AGABI2DRAFT_192278)
SCM : SCHCO_02632766(SCHCODRAFT_02632766)
MLR : MELLADRAFT_123252 MELLADRAFT_123253 MELLADRAFT_48972 MELLADRAFT_88407
SPAR : SPRG_08808 SPRG_12809 SPRG_17535
LMOC : LMOSLCC5850_2342(pepC)
LMW : LMOSLCC2755_2342(pepC)
LMX : LMOSLCC2372_2400(pepC)
LMZ : LMOSLCC2482_2340(pepC)
LMON : LMOSLCC2376_2233(pepC)
LMOS : LMOSLCC7179_2252(pepC)
LMOO : LMOSLCC2378_2342(pepC)
LMOY : LMOSLCC2479_2398(pepC)
LMOT : LMOSLCC2540_2373(pepC)
LMOA : LMOATCC19117_2337(pepC)
LGZ : NCTC10812_02253(pepC)
SPYM : M1GAS476_1434(pepC)
SPH : MGAS10270_Spy1473(pepC)
SPI : MGAS10750_Spy1465(pepC)
SPJ : MGAS2096_Spy1378(pepC)
SPK : MGAS9429_Spy1352(pepC)
STG : MGAS15252_1252(pepC)
STZ : SPYALAB49_001398(pepC)
SNV : SPNINV200_02630(pepC)
SPNG : HMPREF1038_00336(pepC)
SPNE : SPN034156_13550(pepC)
SPNU : SPN034183_03050(pepC)
SPNM : SPN994038_02930(pepC)
SPNO : SPN994039_02940(pepC)
SMC : SmuNN2025_1502(pepC)
SGG : SGGBAA2069_c04140(pepC)
SCP : HMPREF0833_12000(pepC)
SVB : NCTC12167_00266(pepC)
SMEN : SAMEA4412692_1425(pepC)
SCAI : NCTC12191_01103(pepC)
SPSU : NCTC13786_01625(pepC)
SPOC : NCTC10925_01532(pepC)
SAUP : NCTC3168_00271(pepC)
SURN : NCTC13766_00447(pepC)
SVF : NCTC3166_00502(pepC)
LJO : LJ_0395 LJ_0716 LJ_0719
LJF : FI9785_254(pepG) FI9785_256(pepG) FI9785_413(pepC) FI9785_589 FI9785_590 FI9785_591 FI9785_592 FI9785_694(pepE)
LJH : LJP_0182 LJP_0184c LJP_0372c LJP_0528c LJP_0531c LJP_0649
LJN : T285_01000 T285_01010 T285_01875 T285_02755 T285_02770 T285_03450
LAC : LBA0195(pepG) LBA0204(pepE) LBA0343 LBA0911
LAD : LA14_0190 LA14_0199 LA14_0340 LA14_0932
LAF : SD55_0187(pepG) SD55_0196(pepE) SD55_0339 SD55_0932
LBU : LBUL_0241 LBUL_0242 LBUL_1604
LDE : LDBND_0244(pepG) LDBND_0245(pepW) LDBND_1615
LDL : LBU_0223 LBU_0224 LBU_1473
LGA : LGAS_0179 LGAS_0181 LGAS_0334 LGAS_0730
LHE : lhv_0212 lhv_0221 lhv_0363 lhv_0983
LHL : LBHH_0198 LBHH_0201 LBHH_0324 LBHH_1202
LHR : R0052_01120 R0052_01165 R0052_01850 R0052_07070
LHV : lhe_0914(pepCE) lhe_1868(pepE) lhe_1877(pepE2)
LHH : LBH_0167 LBH_0170 LBH_0289 LBH_0810
LHD : HUO_01960 HUO_02010 HUO_02710 HUO_07190
LCR : LCRIS_00198(pepC1) LCRIS_00207(pepC2) LCRIS_00344(pepC3) LCRIS_00964(pepC4)
LAM : LA2_01125 LA2_01170 LA2_01775 LA2_04750
LAI : LAC30SC_00930 LAC30SC_00985 LAC30SC_01695 LAC30SC_04565
LAY : LAB52_01010 LAB52_01065 LAB52_01665 LAB52_04535
LKE : WANG_0043 WANG_0752(pepC4) WANG_1817 WANG_1827
LAW : LACWKB8_1340 LACWKB8_1523
LGL : AO203_01210 AO203_01920 AO203_01950 AO203_07480
LJE : BUE77_01410 BUE77_01420 BUE77_02100
LAMY : B1745_00910 B1745_00955 B1745_01515 B1745_07355
LPW : LpgJCM5343_01800(pepC) LpgJCM5343_01830(pepC) LpgJCM5343_03300(pepC) LpgJCM5343_05120(pepC) LpgJCM5343_06840(pepC)
LKL : DKL58_06800(pepC) DKL58_07835
LAPI : DKL56_05740(pepC) DKL56_06680
LHS : DLD54_06470(pepC) DLD54_07250
LTA : H1A07_00945 H1A07_00980 H1A07_01870(pepC) H1A07_02700 H1A07_02715 H1A07_03315
LUU : H4B44_01140 H4B44_01310 H4B44_02080(pepC)
LPAI : GYM71_07490(pepC) GYM71_08735
LIQ : KBW87_00940 KBW87_00965 KBW87_01705(pepC) KBW87_05515
LCZ : LCAZH_2302 LCAZH_2303
LCS : LCBD_2514(blmH) LCBD_2515
LCW : BN194_24670(pepC) BN194_24680(pepC_2)
LCL : LOCK919_2565 LOCK919_2566
LPQ : AF91_11575 AF91_11580
LPI : LBPG_02255 LBPG_03092
LPAP : LBPC_2258 LBPC_2259
LCB : LCABL_25130(pepC1) LCABL_25140(pepC2)
LCX : LCA12A_1852 LCA12A_1853
LRH : LGG_02345(pepC1) LGG_02346(pepC2)
LRL : LC705_02337(pepC1) LC705_02338(pepC2)
LRA : LRHK_2347(pepE) LRHK_2348
LRO : LOCK900_2307 LOCK900_2308
LRC : LOCK908_2402 LOCK908_2403
LCHI : KG086_10315 KG086_10320
LZE : KG087_11165 KG087_11170
LPL : lp_0601(pepC1) lp_3673(pepC2)
LPJ : JDM1_0540(pepC1) JDM1_2938(pepC2)
LPS : LPST_C0501(pepC1) LPST_C3000(pepC2)
LPR : LBP_cg0482 LBP_cg2937
LPB : SH83_02615 SH83_15245
LPX : ASU28_04060 ASU28_14460
LPG : BB562_13740 BB562_16285
LARG : LPA65_02590 LPA65_15330
LRU : HMPREF0538_21095(pepE) HMPREF0538_21502(pepC)
LRR : N134_01570 N134_09945
LMU : LBLM1_00065 LBLM1_09645
LOR : AYI71_00195 AYI71_08005 AYI71_15430
LVA : LV515_01470 LV515_09090
LFN : LF145_01575 LF145_08500
LPON : LP475_01650 LP475_08310
LPAS : KZE55_02335 KZE55_09675
LPOR : PRK60_07865 PRK60_09035
LNG : BSQ50_00735 BSQ50_02115
LMAL : LM596_01140 LM596_02900
LSN : LSA_04460(pepC) LSA_13430(pepE)
LLE : AYR59_00140 AYR59_01640
LFV : LF543_00725 LF543_06635
FIX : M8332_01575 M8332_06745
FCLI : M3M38_02835 M3M38_04510
FHIN : M3M39_01380 M3M39_07095
FMT : M3M35_04715 M3M35_06315
FCP : M3M37_04500 M3M37_06360
LBN : LBUCD034_1533 LBUCD034_2347(pepC)
LPAR : FAM21731_01149 FAM21731_01972
LCU : PL11_004455 PL11_007580
LKF : DNL43_04365 DNL43_11520
LHIL : G8J22_01811(pepC) G8J22_02630(pepE_1)
LLAO : LHL71_00130 LHL71_04860
LBK : LVISKB_0583 LVISKB_2372
LKO : ABN16_01935 ABN16_05770
LZY : LZ3411_0597 LZ3411_2440
LSUA : H3M12_03150 H3M12_06005
LJI : ELX58_03680 ELX58_07070 ELX58_07735 ELX58_07740
LACA : LAC1533_0523 LAC1533_1708
LSW : GTO87_00675 GTO87_01585
LCY : LC20004_05275 LC20004_12355
LHO : LOOC260_104470 LOOC260_122570
LKU : APS55_01055 APS55_02815 APS55_02820
ABOM : D7I45_00440 D7I45_00445 D7I45_05235
AAPI : MOO46_00020 MOO46_05680 MOO46_05685
LPD : AYR62_08375 AYR62_14100
LMAE : FGL90_04770 FGL90_07260
PCE : PECL_1859(pepE) PECL_371
PDM : ADU72_1174 ADU72_1554
PIO : PI20285_07310 PI20285_09555
LROS : LROSL1_0768 LROSL1_2391
LGN : ABM34_05150 ABM34_09490
LHI : JP39_02380 JP39_12040
LCT : BI355_0048 BI355_0497
LALW : BTM29_00445 BTM29_02680
LALI : LA20249_08080 LA20249_10950
LFM : LF20184_00090 LF20184_09800 LF20184_12365
LZH : D1B17_00230 D1B17_02840
LFT : FG051_09640 FG051_12470
CPAB : G6534_07945 G6534_10470
LSA : LCA_1686(pepC1N) LCA_1687(pepC1C) LCA_1688(pepC2)
LCV : FBA2_01785 FBA2_01790
LGM : LG542_07585 LG542_07590
LBM : DS830_00790 DS830_07690
LHB : D1010_11575 D1010_11580
OEN : DSM07_05605 DSM07_07820
LBE : MOO44_02305 MOO44_03465 MOO44_03470
EFU : HMPREF0351_12197(pepC) HMPREF0351_13050(pepC2)
ECEC : NCTC12421_01118(pepC)
THL : TEH_09450(pepC) TEH_25330
TEY : GLW17_03680 GLW17_06925
TOO : C7K38_01305 C7K38_04500
TKR : C7K43_10645 C7K43_12925
VTE : BHY08_03890 BHY08_09885
VPI : BW732_03310 BW732_06985
VLC : G314FT_06340(pepC_1) G314FT_06380(pepC_2) G314FT_18510(pepC_3)
CLO : HMPREF0868_0823 HMPREF0868_1340
PBQ : C3V37_04420 C3V37_04425
FMS : M1R53_00685 M1R53_00690
PMIC : NW74_01985 NW74_02865
SPOA : EQM13_01935 EQM13_09740
ECOU : M1R54_03240 M1R54_03245
MAMP : MAMA39_04500 MAMA39_04510
MTUY : H3143_02150 H3143_02155
MMIO : HLA92_01990 HLA92_01995
MPHE : HGG69_00350 HGG69_00355
MMAU : NCTC10168_00488(pepC)
MMEL : NCTC10153_00345(pepC)
AAXA : NCTC10138_00831(pepC)
MGA : MGA_0100(pepC_1) MGA_0101(pepC_2)
MGH : MGAH_0100(pepC_1) MGAH_0101(pepC_2)
MGF : MGF_2888(pepC_2) MGF_2898(pepC_1)
MGN : HFMG06NCA_3326(pepC_1) HFMG06NCA_3334(pepC_2)
MGS : HFMG95NCA_3342(pepC_1) HFMG95NCA_3350(pepC_2)
MGT : HFMG01NYA_3404(pepC_1) HFMG01NYA_3412(pepC_2)
MGV : HFMG94VAA_3415(pepC_1) HFMG94VAA_3423(pepC_2)
MGW : HFMG01WIA_3264(pepC_1) HFMG01WIA_3272(pepC_2)
MGAC : HFMG06CAA_3465(pepC_1) HFMG06CAA_3473(pepC_2)
MGAN : HFMG08NCA_3292(pepC_1) HFMG08NCA_3300(pepC_2)
MGNC : HFMG96NCA_3512(pepC_1) HFMG96NCA_3520(pepC_2)
MGZ : GCW_02520(pepC_1) GCW_02525(pepC_2)
MPHC : DMC14_000020 DMC14_000515
MANE : DP065_01250 DP065_01260
MSAI : NCTC10113_01268(pepC)
MOB : NCTC10112_00252(pepC)
MPE : MYPE9890(MYPE9890) MYPE9900(MYPE9900)
MIW : EER00_03035 EER00_03040
CKP : ckrop_0521(pepC2) ckrop_0522(pepC1)
CPG : CP316_01655 CP316_01660
CPP : CpP54B96_0314(pepC1)
CPZ : CpPAT10_0316a(pepC2)
COR : Cp267_0323(pepG) Cp267_0324(pepC1)
COP : CP31_01875 CP31_01880
COI : CPCIP5297_01660 CPCIP5297_01665
COE : CP258_01660 CP258_01665
COU : CP162_01695 CP162_01700
CPSE : CPTA_00841 CPTA_00842
CPSU : CPTB_00690 CPTB_00691
CPSF : CPTC_00352 CPTC_00353
CUL : CULC22_00358(bleo1) CULC22_00359(bleo2)
CUC : CULC809_00354(bleo1) CULC809_00355(bleo2)
CUE : CULC0102_0400 CULC0102_0401
CUN : Cul210932_0373(pepG) Cul210932_0374(pepC)
CUS : CulFRC11_0355(pepG) CulFRC11_0356(pepC)
CUQ : Cul210931_0360(pepG) Cul210931_0361(pepC)
CUZ : Cul05146_0385(pepG) Cul05146_0386(pepC)
CUJ : CUL131002_0357(pepG) CUL131002_0358(pepC)
CTER : A606_09190 A606_09195
CGY : CGLY_04155 CGLY_04160
CPRE : Csp1_07000(pepC_1) Csp1_07010(pepC_2)
CKF : I6I12_02530 I6I12_02535
CGLU : I6J20_03650 I6J20_03655
CTUB : I6I74_03625 I6I74_03630
CGRN : 4412665_01843(pepE)
PFR : PFREUD_06380(pepC) PFREUD_11360(pepC)
PFRE : RM25_0598 RM25_1055
PAUS : NCTC13651_01291(pepC)
PBO : PACID_25010 PACID_33490
AACI : ASQ49_05485 ASQ49_09515
ACIJ : JS278_00167(pepC) JS278_01008(pepE_2)
AJI : C0Z10_00510 C0Z10_03400
NAQU : ENKNEFLB_00660(pepC)
AVC : NCTC10951_02146(pepE)
FVG : I6G91_00290 I6G91_02380
BLO : BL0173(pepC1) BL0321(pepC2)
BLJ : BLD_1079(pepC1) BLD_1534(pepC2)
BLON : BLIJ_0363 BLIJ_2494
BLF : BLIF_0271 BLIF_0272 BLIF_1896
BLB : BBMN68_1084(pepC1) BBMN68_1467(pepC2)
BLK : BLNIAS_00063 BLNIAS_02382
BLZ : BLGT_01915 BLGT_09800
BLX : GS08_01760 GS08_10060
BAD : BAD_0272(pepC2) BAD_0964(pepC1)
BADL : BADO_0280 BADO_1021
BADO : BBMN23_0300 BBMN23_1105
BLA : BLA_0320(pepC) BLA_0418(pepC)
BLC : Balac_0336 Balac_0441
BLT : Balat_0336 Balat_0441
BNM : BALAC2494_00675 BALAC2494_00794
BANI : Bl12_0314 Bl12_0409
BANL : BLAC_01685 BLAC_02210
BNI : BANAN_01740 BANAN_02300
BANM : EN10_01705 EN10_02240
BDE : BDP_0380(pepC2) BDP_1343
BBP : BBPR_0613(pepC) BBPR_1612
BBI : BBIF_0637(pepC1) BBIF_1556(pepC2)
BBF : BBB_0597(pepC) BBB_1591(pepC)
BBV : HMPREF9228_1766 HMPREF9228_1767 HMPREF9228_1919
BBRU : Bbr_1699(pepC1) Bbr_1700(pepC2) Bbr_1818(pepC3)
BBRE : B12L_1628 B12L_1765
BBRV : B689b_1727 B689b_1859
BBRJ : B7017_1894 B7017_2040
BBRC : B7019_1867 B7019_2004
BBRN : B2258_1717 B2258_1718 B2258_1841
BBRS : BS27_1689(pepC1) BS27_1690(pepC2)
BKA : AH68_01545 AH68_05565 AH68_06515
BCAT : BBCT_0253 BBCT_1075
BPSP : AH67_01815 AH67_07185
BANG : BBAG_0298 BBAG_0699
BPSC : BBPC_0282 BBPC_0920
BSCA : BBSC_0454 BBSC_2365
BCHO : BcFMB_00765 BcFMB_04075
BLEM : BL8807_00545 BL8807_05965
BEU : BE0216_05735 BE0216_11045
GVG : HMPREF0421_20285 HMPREF0421_20340
GVA : HMPREF0424_0217 HMPREF0424_1255
GVH : HMPREF9231_1214 HMPREF9231_1272
GPT : K9E43_02595 K9E43_04035
GSW : K9E37_02690 K9E37_04205
OLO : ADJ70_10185 ADJ70_10220
PCAT : Pcatena_12840(pepC)
ATB : J4859_07275 J4859_07280
TIO : INP52_06445 INP52_06450
LCAL : ATTO_08400(pepC) ATTO_13420
TRC : DYE49_04160 DYE49_04165
SNS : VC03_01030 VC03_02685
PPIO : CE91St28_24240(pepC)
BTHO : Btheta7330_02732(pepC_2)
BOA : Bovatus_00501(pepE_1)
BCEL : BcellWH2_00619(pepE_1)
PCRE : NCTC12858_01591(pepC)
PCAG : NCTC12856_02100(pepE_2)
PMER : INE87_03491(pepE_2)
OSP : Odosp_1312 Odosp_2172
BUY : D8S85_08235 D8S85_10870 D8S85_18145
POC : NCTC13071_00048(pepC)
ACOU : A5CBH24_21470 A5CBH24_22440
BLQ : L21SP5_01437(pepC_1) L21SP5_02101
MCOS : GM418_02980 GM418_13125 GM418_15965
MBAS : ALGA_0766 ALGA_2401 ALGA_3062 ALGA_3130
CBAE : COR50_05375 COR50_20955
CHIH : GWR21_10775 GWR21_19185
ARK : D6B99_05925 D6B99_11135
ARAC : E0W69_000755 E0W69_001205
ATEE : K9M52_16085 K9M52_17495
PEP : AQ505_03410 AQ505_20675
PSTY : BFS30_02015 BFS30_08130
PGS : CPT03_01975 CPT03_15210
PGJ : QG516_11860 QG516_20525
SPHN : BV902_02540 BV902_04700
SMIZ : 4412673_00444(pepC)
SPDR : G6053_01540 G6053_03490
SDJ : NCTC13534_00450(pepC)
STHA : NCTC11429_00908(pepC_1) NCTC11429_01288(pepC_2)
SSIY : JVX97_02090 JVX97_28490
FKI : FK004_02735 FK004_11090
FEH : LZF87_04225 LZF87_13175
FCU : NOX80_02455 NOX80_13630
CGH : CGC50_13030 CGC50_13035
MYR : MYRA21_0892 MYRA21_2980
MOD : AS202_04085 AS202_14525
MYZ : BK054_03965 BK054_14175
KOS : KORDIASMS9_04145(pepC)
CGLE : NCTC11432_01781(pepE_2)
EBV : F0358_01395 F0358_03235
EFAL : FH779_13735 FH779_15895
ESTE : HNV03_08940 HNV03_13020
OHO : Oweho_0435 Oweho_1142
SHYR : LA303_07380 LA303_10305
CABY : Cabys_4188 Cabys_488
» show all
Taxonomy
Reference
Authors
Bromme D, Rossi AB, Smeekens SP, Anderson DC, Payan DG
Title
Human bleomycin hydrolase: molecular cloning, sequencing, functional expression, and enzymatic characterization.
Journal
Sequence
Reference
Authors
Adachi H, Tsujimoto M, Fukasawa M, Sato Y, Arai H, Inoue K, Nishimura T.
Title
cDNA cloning and expression of chicken aminopeptidase H, possessing endopeptidase as well as aminopeptidase activity.
Journal
Sequence
Reference
Authors
Zheng W, Johnston SA, Joshua-Tor L.
Title
The unusual active site of Gal6/bleomycin hydrolase can act as a carboxypeptidase, aminopeptidase, and peptide ligase.
Journal
Sequence
Reference
Authors
Mistou MY, Gripon JC.
Title
Catalytic properties of the cysteine aminopeptidase PepC, a bacterial bleomycin hydrolase.
Journal
Sequence
Other DBs
ExPASy - ENZYME nomenclature database: 3.4.22.40