KEGG   ENZYME: 3.4.22.49
Entry
EC 3.4.22.49                Enzyme                                 
Name
separase;
separin
Class
Hydrolases;
Acting on peptide bonds (peptidases);
Cysteine endopeptidases
Reaction(IUBMB)
All bonds known to be hydrolysed by this endopeptidase have arginine in P1 and an acidic residue in P4. P6 is often occupied by an acidic residue or by an hydroxy-amino-acid residue, the phosphorylation of which enhances cleavage
Comment
In both budding yeast and human cells, cleavage of the cohesin subunit Scc1 by separase is required for sister chromatid separation in mitosis. Budding yeast separase is also known to cleave the Rec8 subunit of a meiotic cohesin complex and the kinetochore protein Slk19. Type example of peptidase family C50.
History
EC 3.4.22.49 created 2003
Orthology
K02365  separase
Genes
HSA9700(ESPL1)
PTR451937(ESPL1)
PPS100986315(ESPL1)
GGO101147294(ESPL1)
PON100444516(ESPL1)
NLE100589406(ESPL1)
MCC702136(ESPL1)
MCF102144900(ESPL1)
CSAB103238388 103238391(ESPL1)
CATY105580273(ESPL1)
PANU101019541(ESPL1)
TGE112634147(ESPL1)
RRO104670601(ESPL1)
RBB108521614(ESPL1)
TFN117090041(ESPL1)
PTEH111541935(ESPL1)
CJC100404434(ESPL1)
SBQ101048054(ESPL1)
CSYR103250910(ESPL1)
MMUR105870726(ESPL1)
OGA100964680(ESPL1)
MMU105988(Espl1)
MCAL110310439(Espl1)
MPAH110334838(Espl1)
RNO315330(Espl1)
MCOC116080433(Espl1)
MUN110552298(Espl1)
CGE100768842(Espl1)
PLEU114696628(Espl1)
NGI103738516(Espl1)
HGL101723260(Espl1)
CPOC100733255(Espl1)
CCAN109684045(Espl1)
DORD105997880(Espl1)
DSP122124210(Espl1)
OCU100356105(ESPL1)
OPI101534121(ESPL1)
TUP102498926(ESPL1)
CFA607879(ESPL1)
VVP112921175(ESPL1)
VLG121479643(ESPL1)
AML100467596(ESPL1)
UMR103675668(ESPL1)
UAH113257561(ESPL1)
UAR123794703(ESPL1)
ELK111157921
LLV125106438
MPUF101682674(ESPL1)
ORO101369929(ESPL1)
EJU114225726(ESPL1)
ZCA113922576(ESPL1)
MLX118008799(ESPL1)
FCA101084118(ESPL1)
PYU121038492(ESPL1)
PBG122473473(ESPL1)
PTG102954819(ESPL1)
PPAD109249194(ESPL1)
AJU106986457(ESPL1)
HHV120247237(ESPL1)
BTA506740(ESPL1)
BOM102284437(ESPL1)
BIU109559041(ESPL1)
BBUB102401554(ESPL1)
CHX102177959(ESPL1)
OAS101105898(ESPL1)
ODA120858293(ESPL1)
CCAD122427561(ESPL1)
SSC100153121(ESPL1)
CFR102522390(ESPL1)
CBAI105076279(ESPL1)
CDK105094570(ESPL1)
VPC102526528(ESPL1)
BACU103008542(ESPL1)
LVE103076864(ESPL1)
OOR101286713(ESPL1)
DLE111173946(ESPL1)
PCAD102974477 114486511(ESPL1)
PSIU116760526(ESPL1)
ECB100063779(ESPL1)
EPZ103559419(ESPL1)
EAI106835283(ESPL1)
MYB102259018(ESPL1)
MYD102752810(ESPL1)
MMYO118651695(ESPL1)
MLF102417742(ESPL1)
MNA107528301(ESPL1)
PKL118729068(ESPL1)
HAI109391774(ESPL1)
DRO112318617(ESPL1)
AJM119043512(ESPL1)
PDIC114513150(ESPL1)
PHAS123813111(ESPL1)
MMF118624167(ESPL1)
RFQ117029415(ESPL1)
PALE102895542(ESPL1)
PGIG120602112(ESPL1)
PVP105302660(ESPL1)
RAY107509850(ESPL1)
MJV108405368(ESPL1)
TOD119247985(ESPL1)
SARA101546252(ESPL1)
LAV100671244(ESPL1)
TMU101351580
DNM101439768(ESPL1)
MDO100032809(ESPL1)
GAS123250223(ESPL1)
SHR100921010
PCW110194100(ESPL1)
OAA103169813(ESPL1)
GGA425536(ESPL1)
PCOC116234926(ESPL1)
MGP104916907(ESPL1)
CJO107306949(ESPL1)
NMEL110390892(ESPL1)
ACYG106029392(ESPL1)
AFUL116501724(ESPL1)
TGU115491336(ESPL1)
PMOA120500677(ESPL1)
OTC121333706(ESPL1)
PRUF121363968
GFR102044437(ESPL1)
PHI102109021(ESPL1)
ETL114070102(ESPL1)
FPG101919296(ESPL1)
FCH102059227(ESPL1)
EGZ104132781(ESPL1)
NNI104009170(ESPL1)
ACHC115350895(ESPL1)
AAM106488008(ESPL1)
AROW112961067(ESPL1)
NPD112952278(ESPL1)
DNE112996065(ESPL1)
ASN102377591(ESPL1)
AMJ102574406(ESPL1)
CPOO109323407(ESPL1)
PSS102455533(ESPL1)
CMY102933417(ESPL1)
CPIC101941011(ESPL1)
TST117888594(ESPL1)
CABI116828489(ESPL1)
MRV120390887(ESPL1)
ACS100566013(espl1)
PVT110070448(ESPL1)
SUND121921737(ESPL1)
PBI103063794(ESPL1)
PMUR107288516(ESPL1)
TSR106550895(ESPL1)
PGUT117666488(ESPL1)
VKO123029896(ESPL1)
PMUA114586316(ESPL1)
ZVI118076682(ESPL1)
GJA107118969(ESPL1)
XLA431925(espl1.S) 733221(espl1.L)
XTR100158636(espl1)
NPR108786827(ESPL1)
RTEM120929323(ESPL1)
BBUF120996094(ESPL1)
BGAR122933341(ESPL1)
DRE797406(espl1)
SRX107711058 107740575
SANH107667390 107673592
SGH107582795 107585178
CCAR109063482 109091441
CAUA113094395(espl1)
IPU108271745(espl1)
PHYP113545840(espl1)
SMEO124382852(espl1)
AMEX103046696(espl1)
EEE113575991(espl1)
TRU101077884(espl1)
TNGGSTEN00027237G001
LCO104931366(espl1)
NCC104967237 104967240
CGOB115008801(espl1)
ELY117256249(espl1)
PLEP121956374(espl1)
SLUC116065562(espl1)
ECRA117943793(espl1)
PFLV114554229(espl1)
GAT120834670(espl1)
PPUG119194157(espl1)
MSAM119886141(espl1)
CUD121507034(espl1)
MZE101471736(espl1)
ONL102078894(espl1)
OAU116320386(espl1)
OLA101174584(espl1)
OML112145288(espl1)
XMA102232020(espl1)
XCO114135107(espl1)
XHE116711188(espl1)
PRET103465369(espl1)
PFOR103147647(espl1)
PLAI106954425(espl1)
PMEI106924384(espl1)
GAF122834239(espl1)
CVG107082120(espl1)
CTUL119776667(espl1)
GMU124856662(espl1)
NFU107384036(espl1)
KMR108235600(espl1)
ALIM106518044(espl1)
NWH119412569(espl1)
AOCE111588761(espl1)
CSEM103385986(espl1)
POV109628965(espl1)
SSEN122768258(espl1)
HHIP117761872(espl1)
LCF108881443(espl1)
SDU111221621(espl1)
SLAL111667822(espl1)
XGL120783239(espl1)
HCQ109522611(espl1)
BPEC110158716(espl1)
MALB109956223(espl1)
SASA106565678(espl1)
OTW112217676(espl1)
OMY110494335(espl1)
OGO124003497(espl1)
ONE115143182(espl1)
SALP111966917(espl1)
SNH120061159(espl1)
ELS105017151(espl1)
SFM108918227(espl1)
PKI111858657(espl1)
AANG118211359(espl1)
LOC102696375(espl1)
PSPA121308896(espl1)
ARUT117400942 117967028
LCM102366561(ESPL1)
BFO118432840
BBEL109479122
CIN100185242
SCLV120343948
SPU576865
APLC110975876
SKO102810216
DMEDmel_CG10583(Sse)
DER6544672
DSE6610899
DSIDsimw501_GD13181(Dsim_GD13181)
DYADyak_GE20543
DSR110176236
DPODpse_GA10414
DPE6600318
DMN108151739 108163891 117189158 117189159
DWI6639208
DMODmoj_GI13175
DAZ108613082
DNV108651051
DHE111593099
DVI6623931
LCQ111690719
AGAAgaP_AGAP001503
ACOZ120951549
AARA120894961
AAG5570130
CQUCpipJ_CPIJ011580
CPII120416142
AME100576647
BIM100745045
BTER100652250
CCAL108631249
OBB114879196
SOC105201909
MPHA105828446
AEC105151714
ACEP105618839
PBAR105424685
VEM105561977
HST105191085
DQU106745176
CFO105256588
FEX115243231
LHU105675199
PGC109858810
OBO105277561
PCF106785104
NVI100286823
CSOL105367898
MDL103573942
TCA103313682
DPA109535482
ATD109602160
NVL108562643
BMOR101738030
BMAN114240413
DPLKGM_212784
PMAC106713033
PPOT106109029
PXU106117645
PRAP111000278
ZCE119836169
HAW110371712
TNL113502987 113506526
PXY105381891
API100162113
DNX107171602
AGS114131393
RMD113557111
BTAB109031090
CLEC106665082
NLU111061611
PHUPhum_PHUM080610
FCD110852479
PVM113827917
PJA122266502
HAME121858770
PCLA123761976
PTRU123511977
EAF111706956
ISCIscW_ISCW017453
DSV119455692
RSAN119393682
RMP119172785
VDE111248630
VJA111270901
TUT107361264
DPTE113792053
CSCU111641562
PTEP107454523
SDM118204682
CELCELE_Y47G6A.12(sep-1) CELE_ZK430.5(ZK430.5)
CBRCBG_14956(Cbr-sep-1)
LOALOAG_04930
TSPTsp_02125
HROHELRODRAFT_191468
LGILOTGIDRAFT_230378
PCAN112556453
GAE121384969
HRF124116111
CRG105325911
MYI110463375
PMAX117343195
MMER123559935
OBI106869135
OSN115209522
LAK106162190 106162192 106173788
SMMSmp_167810
OVIT265_15152
EGLEGR_03600
NVE5515603
EPA110254628
ATEN116288580
AMIL114977839
PDAM113669626
DGT114518975 114520908
HMG101239166
AQU105312138
ATHAT4G22970(ESP)
ALY9303812
CRB17878951
CSAT104718008 104727823 104731195
EUSEUTSA_v10024187mg EUTSA_v10029518mg
BRP103860637 103861751
BNA106373062 106375763 106398538 106440466 106440619 111214417
BOE106301587 106316163 106337896 106343049
RSZ108842701
THJ104814230
CPAP110808249
CIT102627230
CICCICLE_v10000008mg
PVY116123826 116136111
MINC123206072 123214667
TCC18607368
GRA105786009
GHI107921536 107925336
GAB108466833
DZI111298142
EGR104455025
GMX100791010 100805306
GSJ114385811 114422230
PVUPHAVU_006G212000g
VRA106775912
VAR108343196
VUN114188679
CCAJ109803269
APRC113852013
MTRMTR_2g007790 MTR_7g023120
CAM101501526
LJALj0g3v0225339.1(Lj0g3v0225339.1)
ADU107468818 110280895
AIP107623888
AHF112714932 112784436 112800459
LANG109334474
FVE101294643
RCN112176206 112176381
PPER18791340
PMUM103319233
PAVI110764872
PDUL117615543
MDM103425344 114823234
ZJU107427758
MNT21406896
CSV101203087
CMO103488503
BHJ120075849
MCHA111017677
CMAX111478544
CMOS111447831
CPEP111795502
RCU8277534
JCU105628784
HBR110633641
MESC110618533
POP7478128
PEU105114008
PALZ118029252
JRE109007053
QSU111987076
QLO115977465
TWL119982436
VVI100259948 104882073
VRI117926741
SLY101263947
SPEN107008385
SOT102604188
CANN107839972
NTA107804022 107828330
NSY104227270
NTO104086993
NAU109243351
INI109169153
ITR116019743
SIND105169434
OEU111411489
EGT105949607
SSPL121753928 121758961
HAN110884718
ECAD122594979
LSV111878317
CCAV112517090
DCR108209499
CSIN114268452
BVG104888210
SOE110783145
CQI110728401 110738838
NNU104589335
MING122086603
TSS122666187
PSOM113346473 113356227
NCOL116266278
OSA4330866
DOSAOs02t0770700-01(Os02g0770700)
OBR102702071
BDI100825113
ATS109766103
TDC119315995 119322508
TAES123128537 123138403 123145658
SBI8073776 8075210
ZMA103654220
SITA101777770 101782294
SVS117839564 117852376
PVIR120643306 120657316
PHAI112882964
PDA103696471
EGU105035069
MUS103974999 103998153
DCT110108353
PEQ110020186
AOF109842386
ATR18424419
SMOSELMODRAFT_405667 SELMODRAFT_442839
PPP112285826
CRECHLRE_01g029200v5
VCNVOLCADRAFT_96349(espA)
CSLCOCSUDRAFT_40112
CVRCHLNCDRAFT_143874
APROF751_1252
OLUOSTLU_119551(cut1)
OTAOT_ostta02g00970
GSLGasu_44590
CCPCHC_T00004631001
SCEYGR098C(ESP1)
AGOAGOS_AAR089C
KLAKLLA0_D15136g
KMXKLMA_50439(ESP1)
LTHKLTH0E05478g
ZROZYRO0C12672g
CGRCAGL0G03223g
NCSNCAS_0E01080(NCAS0E01080)
NDINDAI_0G01210(NDAI0G01210)
TPFTPHA_0D04010(TPHA0D04010)
TBLTBLA_0C02250(TBLA0C02250)
TDLTDEL_0F03240(TDEL0F03240)
TGBHG536_0G01540
KAFKAFR_0K01610(KAFR0K01610)
ZMKHG535_0B06080
PPAPAS_chr1-4_0541
DHADEHA2D01958g
PICPICST_28228(ESP1)
PGUPGUG_04920
SPAASPAPADRAFT_132760
LELLELG_01443
CALCAALFM_C101670CA(ESP1)
CTPCTRG_04463
COTCORT_0B10500
CDUCD36_01530
CTENCANTEDRAFT_94478
YLIYALI0C08960g
CLUCLUG_00834
CLUSA9F13_10g01056 A9F13_10g01067
CAURCJI97_004121
SLBAWJ20_4184(ESP1)
PKZC5L36_0B04650
BNNFOA43_003297
BBRXBRETT_004142
NCRNCU00205
NTENEUTE1DRAFT146400(NEUTE1DRAFT_146400)
SMPSMAC_02224(putative esp1)
PANPODANSg5443
TTTTHITE_2146478
MTMMYCTH_2117988
CTHRCTHT_0070540
MGRMGG_06245
TMNUCRPA7_6398
SSCKSPSK_08265
FGRFGSG_05504
FPUFPSE_03499
FVRFVEG_07021
FOXFOXG_09389
NHENECHADRAFT_46823
TRETRIREDRAFT_50913
TRRM419DRAFT_26189
MAWMAC_02527
MAJMAA_03454
CMTCCM_07539
PLJVFPFJ_01244
VALVDBG_06747
VDAVDAG_05810
CFJCFIO01_11437
SAPOSAPIO_CDS5673
ELAUCREL1_8234
PFYPFICI_08012
SSLSS1G_07342
BFUBCIN_10g02490(Bcesp1)
MBEMBM_02389
PSCOLY89DRAFT_778661
GLZGLAREA_11949
ANIAN8783.2
AFMAFUA_5G09710
ACTACLA_012860
NFINFIA_077600
AORAO090005000276
ANGANI_1_1636064(An07g03090)
AFVAFLA_003064
ALUCAKAW2_11864A
ACHEACHE_60910S
APUUAPUU_70349A
PCSPc12g05830
PDPPDIP_54810
TMFPMAA_052120
TRGTRUGW13939_00088
CIMCIMG_04712
CPWCPC735_071130
UREUREG_01885
PBLPAAG_05691
PBNPADG_07698
ABEARB_01287
TVETRV_01158
AJEHCAG_04674
BGHBDBG_04545
PNOSNOG_00996
PTEPTT_18381
BZECOCCADRAFT_3922
BSCCOCSADRAFT_189437
BORCOCMIDRAFT_105691
AALTCC77DRAFT_979400
ZTRMYCGRDRAFT_92316
PFJMYCFIDRAFT_213087
BCOMBAUCODRAFT_149939
NPAUCRNP2_1130
TMLGSTUM_00009703001
SPOSPCC5E4.04(cut1)
CNECNB00660
CNBCNBB5030 CNBB5040
CGICGB_B9070W CGB_B9080W
TMSTREMEDRAFT_29861
TASAA1Q1_05076
TVSTRAVEDRAFT_41033
DSQDICSQDRAFT_163077
PCOPHACADRAFT_203784
SHSSTEHIDRAFT_76141
HIRHETIRDRAFT_56130
PSQPUNSTDRAFT_102931
ADLAURDEDRAFT_170753
FMEFOMMEDRAFT_171563
GTRGLOTRDRAFT_80657
LBCLACBIDRAFT_297425 LACBIDRAFT_307095 LACBIDRAFT_307111 LACBIDRAFT_317505 LACBIDRAFT_317635 LACBIDRAFT_317789
MRRMoror_3206
CCICC1G_02409
SCMSCHCODRAFT_67598
ABPAGABI1DRAFT80074(AGABI1DRAFT_80074)
ABVAGABI2DRAFT208386(AGABI2DRAFT_208386)
CPUTCONPUDRAFT_138600
SLASERLADRAFT_445696
WSEWALSEDRAFT_60488
WICJ056_003644
UMAUMAG_03262
PFPPFL1_06503
MGLMGL_1370
MRTMRET_3990
PGRPGTG_03501
MLRMELLADRAFT_36075
EINEint_070290
EHEEHEL_070310
EROEROM_070280
MBRMONBRDRAFT_33070
SREPTSG_11533
EHIEHI_120330(182.t00017)
EDIEDI_265930
EIVEIN_059330
ACANACA1_053220
CPVcgd7_2760
CHOChro.70313
TETTTHERM_00297160
PTMGSPATT00003810001
SMINv1.2.024604.t1(symbB.v1.2.024604.t1)
FCYFRACYDRAFT_178623
TPSTHAPSDRAFT_260749
AAFAURANDRAFT_72664
PIFPITG_03497
PSOJPHYSODRAFT_471658
EHXEMIHUDRAFT_451944
GTTGUITHDRAFT_148744
TBRTB927.1.3120
TBGTbgDal_I1900
TCR507837.10 508405.80
LMALMJF_20_1680
LIFLINJ_20_1650
LDOLDBPK_201650
LMILMXM_20_1680
LBZLBRM_20_5840
LPANLPMP_205780
TVATVAG_249880
 » show all
Reference
1  [PMID:12194817]
  Authors
Waizenegger I, Gimenez-Abian JF, Wernic D, Peters JM
  Title
Regulation of human separase by securin binding and autocleavage.
  Journal
Curr Biol 12:1368-78 (2002)
DOI:10.1016/S0960-9822(02)01073-4
  Sequence
[hsa:9700]
Other DBs
ExplorEnz - The Enzyme Database: 3.4.22.49
IUBMB Enzyme Nomenclature: 3.4.22.49
ExPASy - ENZYME nomenclature database: 3.4.22.49
BRENDA, the Enzyme Database: 3.4.22.49
CAS: 351527-77-0

DBGET integrated database retrieval system