KEGG ENZYME: 3.4.22.53

ENZYME: 3.4.22.53

Entry

EC 3.4.22.53                Enzyme

Name

calpain-2;
calcium-activated neutral protease II;
m-calpain;
milli-calpain

Class

Hydrolases;
Acting on peptide bonds (peptidases);
Cysteine endopeptidases

Reaction(IUBMB)

Broad endopeptidase specificity

Comment

Type example of peptidase family C2. Requires Ca2+ at millimolar concentrations for activity. Cytosolic in animal cells. The active enzyme molecule is a heterodimer in which the large subunit contains the peptidase unit, and the small subunit is also a component of EC 3.4.22.52, calpain-1.

History

EC 3.4.22.53 created 1981 as EC 3.4.22.17, transferred 2003 to EC 3.4.22.53

Orthology

K03853

calpain-2

Genes

HSA:	824(CAPN2)

PTR:	457765(CAPN2)

PPS:	100988890(CAPN2)

GGO:	101149206(CAPN2)

PON:	129047251(CAPN2)

PPYG:

129014371(CAPN2)

NLE:	100589114(CAPN2)

HMH:	116457885(CAPN2)

SSYN:

129458153(CAPN2)

MCC:	703047(CAPN2)

MCF:

102122692

MTHB:

126937478

MNI:	105478205(CAPN2)

CSAB:

103230023(CAPN2)

CATY:

105576962(CAPN2)

PANU:

101022030(CAPN2)

TGE:	112619114(CAPN2)

MLEU:

105554489(CAPN2)

RRO:	104677285(CAPN2)

RBB:	108528248(CAPN2)

TFN:	117075199(CAPN2)

PTEH:

111537094(CAPN2)

CANG:

105502824(CAPN2)

CJC:	100405215(CAPN2)

SBQ:	101035587(CAPN2)

CIMI:

108302703(CAPN2)

ANAN:

105722301(CAPN2)

CSYR:

103272581(CAPN2)

MMUR:

105863767

LCAT:

123627504(CAPN2)

PCOQ:

105810138(CAPN2)

OGA:	100946093(CAPN2)

MMU:	12334(Capn2)

MCAL:

110296563(Capn2)

MPAH:

110321506(Capn2)

RNO:	29154(Capn2)

MCOC:

116068107(Capn2)

ANU:	117721391(Capn2)

ASYL:

127697221(Capn2)

MUN:	110546658(Capn2)

CGE:	100771239(Capn2)

MAUA:

101833693(Capn2)

PROB:

127223719(Capn2)

PLEU:

114684418(Capn2)

MORG:

121435859(Capn2)

MFOT:

126502085

AAMP:

119799550(Capn2)

NGI:	103726266(Capn2)

HGL:	101720177(Capn2)

CPOC:

100725761(Capn2)

CCAN:

109677038(Capn2)

DORD:

105984946(Capn2)

DSP:	122109365(Capn2)

DMER:

138827295 138827445

PLOP:

125360104(Capn2)

NCAR:

124961543

MMMA:

107140516(Capn2)

ITI:	101956753(Capn2)

OCU:	100009092(CAPN2)

OPI:	101532667(CAPN2)

TUP:	102481316(CAPN2)

GVR:	103607537(CAPN2)

CFA:	480118(CAPN2)

CLUD:

112648848(CAPN2)

VVP:	112923580(CAPN2)

VLG:	121485762(CAPN2)

NPO:	129509084(CAPN2)

AML:	100476150(CAPN2)

UMR:	103669131(CAPN2)

UAH:	113262899(CAPN2)

UAR:	123791973(CAPN2)

ELK:

111150685

LLV:

125085781

MPUF:

101675244(CAPN2)

MNP:	132025535(CAPN2)

MLK:	131814739(CAPN2)

NVS:	122918704(CAPN2)

ORO:	101364547(CAPN2)

EJU:	114215532(CAPN2)

ZCA:	113932475(CAPN2)

MLX:	118012992(CAPN2)

NSU:	110571384(CAPN2)

LWW:	102747421(CAPN2)

FCA:	101083231(CAPN2)

PYU:	121015188(CAPN2)

PBG:	122475955(CAPN2)

PVIV:

125159604(CAPN2)

LRUF:

124510321

LGF:	123585312(CAPN2)

AJU:

106983173

PPAD:

109258888(CAPN2)

PUC:

125925062

PLEZ:

122212404(CAPN2)

HHV:	120234148(CAPN2)

BTA:	281662(CAPN2)

BOM:	102276298(CAPN2)

BIU:	109570370(CAPN2)

BBUB:

102415122(CAPN2)

BBIS:

104997873

CHX:	102181227(CAPN2)

OAS:	100127211(CAPN2)

BTAX:

128061180(CAPN2)

CSUM:

138090405(CAPN2)

ODA:	120880362(CAPN2)

CCAD:

122452395(CAPN2)

MREE:

136170011(CAPN2)

OVR:	110128640(CAPN2)

MBEZ:

129551654(CAPN2)

SSC:	397393(CAPN2)

CFR:	102512386(CAPN2)

CBAI:

105083948(CAPN2)

CDK:	105099835(CAPN2)

VPC:	102524736(CAPN2)

BACU:

103007207(CAPN2) 103019749

BMUS:

118883081(CAPN2)

LVE:	103080579(CAPN2)

OOR:	101270285(CAPN2)

LALB:

132507824(CAPN2)

GMF:	115859440(CAPN2)

DDP:

132414909

DLE:	111177507(CAPN2)

PCAD:

102976041(CAPN2)

PSIU:

116740338(CAPN2)

PPHC:

136120627(CAPN2)

NASI:

112403123(CAPN2)

ECB:	100055184(CAPN2)

EPZ:	103563904(CAPN2)

EAI:	106829535(CAPN2)

MYB:	102240228(CAPN2)

MYD:	102769788(CAPN2)

MMYO:

118678942(CAPN2)

MLF:

102438585

MDT:	132222451(CAPN2)

MYUM:

138999149(CAPN2)

PKL:	118727621(CAPN2)

EFUS:

103293526(CAPN2)

MNA:	107543584(CAPN2)

DRO:

112318759

SHON:

119003139

AJM:

119036522

PDIC:

114512462

PHAS:

123832759(CAPN2)

MMF:	118640556(CAPN2)

PPAM:

129072993(CAPN2)

HAI:	109381194(CAPN2)

RFQ:	117018599(CAPN2)

PALE:

102897695(CAPN2)

PGIG:

120619310(CAPN2)

PVP:	105292618(CAPN2)

RAY:	107498969(CAPN2)

MJV:	108400832(CAPN2)

TOD:	119244503(CAPN2)

SARA:

101557601(CAPN2)

SFUM:

130039425(CAPN2)

SETR:

126013535(CAPN2)

LAV:	100663804(CAPN2)

TMU:

101341399

ETF:	101648937(CAPN2)

DNM:	101424691(CAPN2)

MDO:	100018672(CAPN2)

GAS:	123244336(CAPN2)

SHR:	100922649(CAPN2)

AFZ:

127559523

PCW:	110205536(CAPN2)

TVP:	118846451(CAPN2)

PBRV:

138141764(CAPN2)

MLAO:

141513183(CAPN2)

OAA:	100081577(CAPN2)

TACU:

119940722(CAPN2)

GGA:	395963(CAPN2)

PCOC:

116230900(CAPN2)

MGP:	100546180(CAPN2)

CJO:	107311033(CAPN2)

TPAI:

128076189(CAPN2)

LMUT:

125690047(CAPN2)

NMEL:

110395702

APLA:

101799042(CAPN2)

ACYG:

106031621

CATA:

118252991

AFUL:

116487638

TGU:

100225408

LSR:

110483833

SCAN:

103819275(CAPN2)

PMOA:

120505963(CAPN2)

OTC:	121346915(CAPN2)

PRUF:

121357014(CAPN2)

ATRC:

139587704(CAPN2)

GFR:	102045030(CAPN2)

FAB:	101809391(CAPN2)

OMA:	130250773(CAPN2)

PHI:	102111446(CAPN2)

PMAJ:

107202529(CAPN2)

CCAE:

111926249(CAPN2)

CCW:	104685555(CAPN2)

CBRC:

103612864(CAPN2)

ACOE:

138107701(CAPN2)

ETL:	114073709(CAPN2)

ZAB:	102073190(CAPN2)

ZLE:	135446046(CAPN2)

ACHL:

103797775

SVG:	106853912(CAPN2)

MMEA:

130583044(CAPN2)

HRT:	120750694(CAPN2)

SATI:

136359542(CAPN2)

CCIC:

134041972(CAPN2)

FPG:	101919959(CAPN2)

FCH:	102048431(CAPN2)

CCRI:

104159172(CAPN2)

NNT:

104408713

SHAB:

115613876

ACUN:

113477955(CAPN2)

TALA:

104365796

ACHC:

115349962(CAPN2)

HALD:

104317978(CAPN2)

HLE:	104838213(CAPN2)

AGEN:

126052620

HHAR:

128149890

GCL:

127015289

CSTI:

104550979(CAPN2)

LDI:

104350930

MNB:	103769082(CAPN2)

DPUB:

104300913(CAPN2)

PPUS:

135186653(CAPN2)

AVIT:

104268653

BRHI:

104489251(CAPN2)

EGZ:	104124542(CAPN2)

NNI:	104019299(CAPN2)

PCRI:

104030145

PCAO:

104045095

PADL:

103925056(CAPN2)

AFOR:

103894459(CAPN2)

FGA:	104081233(CAPN2)

GSTE:

104255472(CAPN2)

CBOY:

140649584(CAPN2)

CLV:

102084552

CNIC:

135987766

MUI:

104544144

PGUU:

104471956

PLET:

104620768

EHS:	104514071(CAPN2)

CMAC:

104483676(CAPN2)

CUCA:

104067050(CAPN2)

TEO:

104376259

BREG:

104630494(CAPN2)

OHA:	104332003(CAPN2)

ACAR:

104526948

CPEA:

104386198(CAPN2)

CVF:	104294692(CAPN2)

RTD:	128906890(CAPN2)

CRID:

134512660(CAPN2)

AAM:	106482724(CAPN2)

AROW:

112963405

NPD:	112954803(CAPN2)

TGT:	104570282(CAPN2)

DNE:	112980095(CAPN2)

SCAM:

104151845(CAPN2)

ASN:	102373508(CAPN2)

AMJ:	102560432(CAPN2)

CPOO:

109319765(CAPN2)

GGN:	109296890(CAPN2)

PSS:	102458713(CAPN2)

CMY:	102937008(CAPN2)

CCAY:

125633672

DCC:	119852740(CAPN2)

CPIC:

101940996(CAPN2)

TST:	117874491(CAPN2) 117888892

CABI:

116825598(CAPN2)

MRV:	120388744(CAPN12) 120400953(CAPN2)

ACS:	100561808(capn2)

ASAO:

132761385(CAPN2)

PVT:	110075406(CAPN2)

SUND:

121919237(CAPN2)

PBI:

103056946

PMUR:

107288074(CAPN2)

CTIG:

120307212(CAPN2)

TSR:	106549641(CAPN2)

PGUT:

117671750(CAPN2)

PCAE:

139320862(CAPN2)

APRI:

131189346

PTEX:

113433240(CAPN2)

NSS:	113421716(CAPN2)

VKO:

123024724

PMUA:

114594632(CAPN2)

PRAF:

128411079 128418951(CAPN12)

ZVI:	118083208(CAPN2)

HCG:	128332565(CAPN12) 128350261

GJA:	107121636(CAPN2)

STOW:

125436868

EMC:	129330026(CAPN2) 129342991 129343726

XLA:	100127267(XB5844015.L) 108716477(capn8.5.L) 108717697(capn2.S) 399075(capn2.L)

XTR:	100379861(capn8.5) 100494922 100495980 448035(capn2)

NPR:	108786661 108787073(CAPN2)

RTEM:

120913392 120937867

ACAT:

141107755 141140828(CAPN2)

BBUF:

120977198 120999612(CAPN2)

BGAR:

122934009(CAPN2)

DTC:	141051967 141060116(CAPN2)

PWL:	138259658(CAPN12) 138295492

MUO:	115465480(CAPN2) 115481010

GSH:

117356530

DRE:	337730(capn8) 445091(capn2l) 541374(capn2a) 563053(capn2b)

SRX:	107722658 107722663 107722669 107724584 107734257 107755343 107755344 107755345 107755925

SANH:

107655329 107655374 107655376 107655378 107659324 107659328 107676365 107679193

SGH:	107549147 107565171 107565172 107565180 107566058 107569122 107571859 107577564 107586086

CCAR:

109046473(capn2l) 109047015(capn2b) 109057483 109062020 109070306 109076939 109100541(capn2a) 109111554

CAUA:

113039948 113039949 113039950 113039951 113065066 113065067 113065139 113065999 113092354 113112426

CGIB:

127942702 127943373 127943374 127956591 127970457 127988414 127988415 127988417 128025938

PTET:

122327143 122327282(capn2b) 122327288 122327868 122327869 122328220(capn2l) 122356702(capn2a)

LROH:

127153354(capn2b) 127159477(capn2l) 127159478 127159480 127175321(capn2a)

GRF:	141325177(capn2a) 141339041(capn8) 141339156 141339307(capn2b) 141339309(capn2l)

OMC:	131530830(capn8) 131531102(capn2l) 131531156 131551993(capn2a)

PPRM:

120479402(capn2a) 120495254(capn8) 120495255(capn2b) 120495447(capn2l)

RKG:	130083184(capn2a) 130103527(capn2l) 130103528(capn8)

MAMB:

125263723(capn2l) 125263724(capn8) 125263725 125263726 125263728 125263731(capn2b) 125276707(capn2a)

CIDE:

127520139 127520684 127521576 127521577 127524777

CERY:

137020441(capn2a) 137027817(capn2b) 137027820 137029203 137029204 137029205 137029208 137029209(capn2l)

MASI:

127412206 127413959 127438536 127438537 127438758 127438759

TROS:

130548323(capn2b) 130548655 130548689 130548690(capn2l) 130550965

TDW:	130429403(capn2b) 130429719 130429728 130430172(capn2l) 130431461(capn2a)

MANU:

129415666(capn2a) 129452865(capn2b) 129453082 129453423(capn2l) 129453424 129453425 129453426 129453427 129453428 129453429 129453430 129453432 129453433 129453436 129453438 141359517 141359519

PDAB:

135741001(capn2b) 135741004(capn2l) 135741005 135741006 135741008 135741009 135741010 135741131 135741132 135786616(capn2a)

IPU:	108259404 108259406 108259420

IFU:	128602567 128602877 128602878

PHYP:

113547581

SMEO:

124379814 124380107(capn8)

TFD:	113656951 113657035 113657038 113657047 113657081 113657082

TVC:	132838552 132839063(capn8)

CGAR:

128518526(capn8) 128518527 128518529 128518556

NGRA:

132874911(capn8) 132874912

TRN:	134308116 134314954(capn2a) 134334283 134334752 134334783

AMEX:

103024279(capn8) 103026146(capn2b) 107197131(capn2a)

CMAO:

118804562 118804578 118804588 118804589 118804595 118804599 118804628 118810905(capn2a)

EEE:	113577726 113581471 113581475

CHAR:

105896912 105910532 116219353 122129548 122129552 122129555 122132274

SPIL:

134061589 134061715(capn8) 134064510 134064515 134100748

TRU:	101064754 101076184 101076595

TFS:	130527429 130533559 130533560

TNG:	GSTEN00008927G001 GSTEN00030802G001 GSTEN00030804G001

LCO:	104918327 104918337 104934896 104934897 104934898

NCC:	104963449 104963450

TBEN:

117499311 117499313 117499673(capn8) 117499674

PGEO:

117441997 117459873 117459874

GACU:

117535626 117553009 117553016

EMAC:

134858433(capn8) 134858435 134862697 134862887 134863186

ELY:	117248470 117248471 117255565 117255600 117255876

EFO:	125885996(capn8) 125885997 125885998 125903649 125903650

PLEP:

121942132

SLUC:

116034148 116049140 116049141 116049166 116049190 116051494 116058023 116058030 116058374 116058375 116058502 118496042

ECRA:

117960009

ESP:

116704839

PFLV:

114556645 114556646 114556744 114557816 114557817 114557979 114557980 114563664 114563675 114566584 114572526

GAT:	120824594 120824596

PPUG:

119214540 119214541 119217601(capn2l) 119217602(capn2b)

AFB:	129092103 129092104 129095536 129095538 129095695

CLUM:

117729021 117743953 117743954

PSWI:

130191238 130191246 130206983 130207436

CANA:

137780110 137780111 137801993 137801994 137801995

CVE:	137866671 137866673 137866703(capn2l) 137882927 137882980

MSAM:

119899880(capn2l) 119899881(capn8) 119899882 119917657

SCHU:

122872848(capn8) 122872850 122884013

CUD:	121508149 121508172 121510754 121510929

SPUL:

138588182 138588979 138594452 138595237

LMIX:

132975131 132975132 132993622 132993654

ALAT:

119019705(capn2l) 119019734(capn8) 119019742(capn2b) 119033589 119034167

ASTR:

137600544 137600545 137600546 137603665 137603667

MZE:	101466554 101468385

ONL:	100706186 100709493 102083160

OAU:	116317696 116317707(capn8) 116317874(capn2l) 116318507

OLA:	101162579 101164919 101165172

OML:	112143530 112162134 112162135

CSAI:

133445752 133445763 133463976 133464168

XMA:	102227133 102234145

XCO:	114138434 114138435 114144102

XHE:	116713621 116713624 116720104

PRET:

103473154 103477212 103477213

PFOR:

103130124 103134145 103134146

PLAI:

106959257 106959267 106961194

PMEI:

106929428 106929429 106931777

GAF:	122829988 122842935 122842936

PPRL:

129356041 129359660(capn8) 129359661

CVG:	107082647 107082675 107102256 107102257

CTUL:

119785494 119785503 119797741 119797749

GMU:	124858622 124868214

NFU:	107375570 107393875

KMR:	108234077 108234124 108251143 108251144(capn2b)

ALIM:

106515918(capn11) 106536239

NWH:	119411348 119411359 119422313

AOCE:

111568891 111568893 111568895 111585239 111585240

EJA:	138195508 138195509 138208769 138209706

BFRE:

138616095 138616096 138634836 138634890

MCEP:

125004579(capn8) 125004580(capn2l) 125004581(capn2b) 125019146 125019147

CSEM:

103383278 103383281 103387544 103387545

POV:	109630641 109632323 109647469 109647544

SSEN:

122762072 122771004 122781965 122781966

SSOE:

131466793 131467261

HHIP:

117763891(capn2l) 117763907(capn8) 117763923 117775632 117776037 117776080

HSP:	118119322 118119434 118119503 118119505 118120876

PPLT:

128437470 128453113 128453899(capn8)

SMAU:

118283772 118283773 118312232 118312235(capn2l) 118312236(capn2b)

LCF:	108877132 108877133(capn8) 108882289

SDU:	111220638 111220639 111237555 111237556 111237557

SLAL:

111649592 111658604 111669521 111669522

XGL:	120796274 120796275 120800453(capn2l) 120800454(capn8) 120800455(capn2b)

HCQ:	109516162 109527604

SSCV:

125968492 125978751

SBIA:

133505698 133509944

PEE:	133403937 133410220 133410221

PTAO:

133476952 133485740 133485741

BPEC:

110156974 110156975 110166012

ENY:	140350952 140351565 140365137 140375899

MALB:

109960723 109971792 109971799 109971826

BSPL:

114857498 114857509(capn2b) 114870532 114870586 114870587

SJO:	128357669 128373143 128373144 128375865 128378195

SSCO:

133976931 133992285 133992369 134000275

GMH:	115539934 115539935 115559787

SASA:

106565007 106565009 106578800 106578801 106584767 106589959 106589985

STRU:

115155631 115155640 115201755 115201756 115208449 115208455

OTW:	112223208 112223950 112236240 112266430(capn8) 112266436

OMY:	100135837 100136224(capn2) 110491224 110491235 110493820(capn8)

OGO:	124002438 124003579(capn8) 124039071 124048054 124048056

ONE:	115107157 115122462 115126231 115142697 115142699

OKI:	109879240 109880977 109890699 109890707 116355398

OKE:	118376416 118379311 118379869 118389064 118389067(capn8)

OMM:	135505262 135505264 135539481

OCLA:

139367880 139367881 139369375 139370607 139377011

SALP:

111960924 111960936

SNH:	120022367(capn8) 120029335 120045314 120045401 120064858

CCLU:

121555339 121572329 121572330(capn8) 121580572 121580650 123484545

ELS:	105028009 105028010 105031125

OEP:	134008523 134008524(capn8) 134022287

SFM:	108927097 108927098 108927128 108933713 108933714

PKI:	111835677 111841738 111852308 111852340 111852381 111852382

AANG:

118212877 118212878 118217793(capn2a) 118218445 118218446(capn8) 118220682 118220959

AROT:

135239032 135245073(capn8) 135245074(capn2a) 135245220 135245571 135249567

CCOG:

133115141(capn8) 133116027 133116505 133118029 133118365 133118366

LOC:	102683469 102686084 102686287

PSPA:

121315710 121315712 121316792 121316797 121316798

ARUT:

117402852 117403231 117403377 117410845 117410846 117411201

PSEX:

120516279(capn8) 120516295(capn2l) 120516636 120516907

LCM:	102347065(CAPN2)

CMK:

103177931

HOC:	132819701 132830514

LERI:

129699624

HAKA:

140730337 140733023

PMRN:

116943157

AGIF:

122847603 122849323

TPAL:

117639677

PVUL:

126817811

GAE:	121379756 121386152 121386313

HRF:	124115873 124136697 124145344

HRJ:	124274220 124291720

HASI:

137256212 137256542 137276711

HCRA:

139459127 139464408 139470348

CVN:	111110708 111127234

OED:

125666590

AIRR:

138329990

MMER:

123566327

RPHI:

132718623 132745282

DPOL:

127877971

MAEA:

128228716

OSN:

115209388

LJP:

135482163

» show all

Reference

1 [PMID:10639123]

Authors

Strobl S, Fernandez-Catalan C, Braun M, Huber R, Masumoto H, Nakagawa K, Irie A, Sorimachi H, Bourenkow G, Bartunik H, Suzuki K, Bode W.

Title

The crystal structure of calcium-free human m-calpain suggests an electrostatic switch mechanism for activation by calcium.

Journal

Proc Natl Acad Sci U S A 97:588-92 (2000)
DOI:10.1073/pnas.97.2.588

Sequence

[hsa:824]

Reference

2 [PMID:12014988]

Authors

Dutt P, Spriggs CN, Davies PL, Jia Z, Elce JS

Title

Origins of the difference in Ca2+ requirement for activation of mu- and m-calpain.

Journal

Biochem J 367:263-9 (2002)
DOI:10.1042/BJ20020485

Other DBs

ExplorEnz - The Enzyme Database:

3.4.22.53

IUBMB Enzyme Nomenclature:

3.4.22.53

ExPASy - ENZYME nomenclature database:

3.4.22.53

BRENDA, the Enzyme Database:

3.4.22.53

CAS:	702693-80-9

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Gene (772)   
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Protein sequence (2159)   
   UniProt (2100)   
   SWISS-PROT (11)   
   RefSeq(pep) (27)   
   PDBSTR (18)   
   PMD (3)   
DNA sequence (34)   
   RefSeq(nuc) (30)   
   GenBank (4)   
3D Structure (7)   
   PDB (7)   
Protein domain (13)   
   InterPro (12)   
   PROSITE(DOC) (1)   
All databases (2985)   

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