KEGG ENZYME: 3.4.22.53

ENZYME: 3.4.22.53

Entry

EC 3.4.22.53                Enzyme

Name

calpain-2;
calcium-activated neutral protease II;
m-calpain;
milli-calpain

Class

Hydrolases;
Acting on peptide bonds (peptidases);
Cysteine endopeptidases

Reaction(IUBMB)

Broad endopeptidase specificity

Comment

Type example of peptidase family C2. Requires Ca2+ at millimolar concentrations for activity. Cytosolic in animal cells. The active enzyme molecule is a heterodimer in which the large subunit contains the peptidase unit, and the small subunit is also a component of EC 3.4.22.52, calpain-1.

History

EC 3.4.22.53 created 1981 as EC 3.4.22.17, transferred 2003 to EC 3.4.22.53

Orthology

K03853

calpain-2

Genes

HSA:	824(CAPN2)

PTR:	457765(CAPN2)

PPS:	100988890(CAPN2)

GGO:	101149206(CAPN2)

PON:	129047251(CAPN2)

PPYG:

129014371(CAPN2)

NLE:	100589114(CAPN2)

HMH:	116457885(CAPN2)

SSYN:

129458153(CAPN2)

MCC:	703047(CAPN2)

MCF:

102122692

MTHB:

126937478

MNI:	105478205(CAPN2)

CSAB:

103230023(CAPN2)

CATY:

105576962(CAPN2)

PANU:

101022030(CAPN2)

TGE:	112619114(CAPN2)

MLEU:

105554489(CAPN2)

RRO:	104677285(CAPN2)

RBB:	108528248(CAPN2)

TFN:	117075199(CAPN2)

PTEH:

111537094(CAPN2)

CANG:

105502824(CAPN2)

CJC:	100405215(CAPN2)

SBQ:	101035587(CAPN2)

CIMI:

108302703(CAPN2)

ANAN:

105722301(CAPN2)

CSYR:

103272581(CAPN2)

MMUR:

105863767

LCAT:

123627504(CAPN2)

PCOQ:

105810138(CAPN2)

OGA:	100946093(CAPN2)

MMU:	12334(Capn2)

MCAL:

110296563(Capn2)

MPAH:

110321506(Capn2)

RNO:	29154(Capn2)

MCOC:

116068107(Capn2)

ANU:	117721391(Capn2)

ASYL:

127697221(Capn2)

MUN:	110546658(Capn2)

CGE:	100771239(Capn2)

MAUA:

101833693(Capn2)

PROB:

127223719(Capn2)

PLEU:

114684418(Capn2)

MORG:

121435859(Capn2)

MFOT:

126502085

AAMP:

119799550(Capn2)

NGI:	103726266(Capn2)

HGL:	101720177(Capn2)

CPOC:

100725761(Capn2)

CCAN:

109677038(Capn2)

DORD:

105984946(Capn2)

DSP:	122109365(Capn2)

PLOP:

125360104(Capn2)

NCAR:

124961543

MMMA:

107140516(Capn2)

ITI:	101956753(Capn2)

OCU:	100009092(CAPN2)

OPI:	101532667(CAPN2)

TUP:	102481316(CAPN2)

GVR:	103607537(CAPN2)

CFA:	480118(CAPN2)

CLUD:

112648848(CAPN2)

VVP:	112923580(CAPN2)

VLG:	121485762(CAPN2)

NPO:	129509084(CAPN2)

AML:	100476150(CAPN2)

UMR:	103669131(CAPN2)

UAH:	113262899(CAPN2)

UAR:	123791973(CAPN2)

ELK:

111150685

LLV:

125085781

MPUF:

101675244(CAPN2)

MNP:	132025535(CAPN2)

MLK:	131814739(CAPN2)

NVS:	122918704(CAPN2)

ORO:	101364547(CAPN2)

EJU:	114215532(CAPN2)

ZCA:	113932475(CAPN2)

MLX:	118012992(CAPN2)

NSU:	110571384(CAPN2)

LWW:	102747421(CAPN2)

FCA:	101083231(CAPN2)

PYU:	121015188(CAPN2)

PBG:	122475955(CAPN2)

PVIV:

125159604(CAPN2)

LRUF:

124510321

LGF:	123585312(CAPN2)

AJU:

106983173

PPAD:

109258888(CAPN2)

PUC:

125925062

PLEZ:

122212404(CAPN2)

HHV:	120234148(CAPN2)

BTA:	281662(CAPN2)

BOM:	102276298(CAPN2)

BIU:	109570370(CAPN2)

BBUB:

102415122(CAPN2)

BBIS:

104997873

CHX:	102181227(CAPN2)

OAS:	100127211(CAPN2)

BTAX:

128061180(CAPN2)

CSUM:

138090405(CAPN2)

ODA:	120880362(CAPN2)

CCAD:

122452395(CAPN2)

MREE:

136170011(CAPN2)

OVR:	110128640(CAPN2)

MBEZ:

129551654(CAPN2)

SSC:	397393(CAPN2)

CFR:	102512386(CAPN2)

CBAI:

105083948(CAPN2)

CDK:	105099835(CAPN2)

VPC:	102524736(CAPN2)

BACU:

103007207(CAPN2) 103019749

BMUS:

118883081(CAPN2)

LVE:	103080579(CAPN2)

OOR:	101270285(CAPN2)

LALB:

132507824(CAPN2)

DLE:	111177507(CAPN2)

PCAD:

102976041(CAPN2)

PSIU:

116740338(CAPN2)

PPHC:

136120627(CAPN2)

NASI:

112403123(CAPN2)

ECB:	100055184(CAPN2)

EPZ:	103563904(CAPN2)

EAI:	106829535(CAPN2)

MYB:	102240228(CAPN2)

MYD:	102769788(CAPN2)

MMYO:

118678942(CAPN2)

MLF:

102438585

MDT:	132222451(CAPN2)

MYUM:

138999149(CAPN2)

PKL:	118727621(CAPN2)

EFUS:

103293526(CAPN2)

MNA:	107543584(CAPN2)

DRO:

112318759

SHON:

119003139

AJM:

119036522

PDIC:

114512462

PHAS:

123832759(CAPN2)

MMF:	118640556(CAPN2)

PPAM:

129072993(CAPN2)

HAI:	109381194(CAPN2)

RFQ:	117018599(CAPN2)

PALE:

102897695(CAPN2)

PGIG:

120619310(CAPN2)

PVP:	105292618(CAPN2)

RAY:	107498969(CAPN2)

MJV:	108400832(CAPN2)

TOD:	119244503(CAPN2)

SARA:

101557601(CAPN2)

SFUM:

130039425(CAPN2)

SETR:

126013535(CAPN2)

LAV:	100663804(CAPN2)

TMU:

101341399

ETF:	101648937(CAPN2)

DNM:	101424691(CAPN2)

MDO:	100018672(CAPN2)

GAS:	123244336(CAPN2)

SHR:	100922649(CAPN2)

AFZ:

127559523

PCW:	110205536(CAPN2)

TVP:	118846451(CAPN2)

PBRV:

138141764(CAPN2)

OAA:	100081577(CAPN2)

TACU:

119940722(CAPN2)

GGA:	395963(CAPN2)

PCOC:

116230900(CAPN2)

MGP:	100546180(CAPN2)

CJO:	107311033(CAPN2)

TPAI:

128076189(CAPN2)

LMUT:

125690047(CAPN2)

NMEL:

110395702

APLA:

101799042(CAPN2)

ACYG:

106031621

CATA:

118252991

AFUL:

116487638

TGU:

100225408

LSR:

110483833

SCAN:

103819275(CAPN2)

PMOA:

120505963(CAPN2)

OTC:	121346915(CAPN2)

PRUF:

121357014(CAPN2)

ATRC:

139587704(CAPN2)

GFR:	102045030(CAPN2)

FAB:	101809391(CAPN2)

OMA:	130250773(CAPN2)

PHI:	102111446(CAPN2)

PMAJ:

107202529(CAPN2)

CCAE:

111926249(CAPN2)

CCW:	104685555(CAPN2)

CBRC:

103612864(CAPN2)

ACOE:

138107701(CAPN2)

ETL:	114073709(CAPN2)

ZAB:	102073190(CAPN2)

ZLE:	135446046(CAPN2)

ACHL:

103797775

SVG:	106853912(CAPN2)

MMEA:

130583044(CAPN2)

HRT:	120750694(CAPN2)

SATI:

136359542(CAPN2)

CCIC:

134041972(CAPN2)

FPG:	101919959(CAPN2)

FCH:	102048431(CAPN2)

CCRI:

104159172(CAPN2)

NNT:

104408713

SHAB:

115613876

ACUN:

113477955(CAPN2)

TALA:

104365796

ACHC:

115349962(CAPN2)

HALD:

104317978(CAPN2)

HLE:	104838213(CAPN2)

AGEN:

126052620

HHAR:

128149890

GCL:

127015289

CSTI:

104550979(CAPN2)

LDI:

104350930

MNB:	103769082(CAPN2)

DPUB:

104300913(CAPN2)

PPUS:

135186653(CAPN2)

AVIT:

104268653

BRHI:

104489251(CAPN2)

EGZ:	104124542(CAPN2)

NNI:	104019299(CAPN2)

PCRI:

104030145

PCAO:

104045095

PADL:

103925056(CAPN2)

AFOR:

103894459(CAPN2)

FGA:	104081233(CAPN2)

GSTE:

104255472(CAPN2)

CBOY:

140649584(CAPN2)

CLV:

102084552

CNIC:

135987766

MUI:

104544144

PGUU:

104471956

PLET:

104620768

EHS:	104514071(CAPN2)

CMAC:

104483676(CAPN2)

CUCA:

104067050(CAPN2)

TEO:

104376259

BREG:

104630494(CAPN2)

OHA:	104332003(CAPN2)

ACAR:

104526948

CPEA:

104386198(CAPN2)

CVF:	104294692(CAPN2)

RTD:	128906890(CAPN2)

CRID:

134512660(CAPN2)

AAM:	106482724(CAPN2)

AROW:

112963405

NPD:	112954803(CAPN2)

TGT:	104570282(CAPN2)

DNE:	112980095(CAPN2)

SCAM:

104151845(CAPN2)

ASN:	102373508(CAPN2)

AMJ:	102560432(CAPN2)

CPOO:

109319765(CAPN2)

GGN:	109296890(CAPN2)

PSS:	102458713(CAPN2)

CMY:	102937008(CAPN2)

CCAY:

125633672

DCC:	119852740(CAPN2)

CPIC:

101940996(CAPN2)

TST:	117874491(CAPN2) 117888892

CABI:

116825598(CAPN2)

MRV:	120388744(CAPN12) 120400953(CAPN2)

ACS:	100561808(capn2)

ASAO:

132761385(CAPN2)

PVT:	110075406(CAPN2)

SUND:

121919237(CAPN2)

PBI:

103056946

PMUR:

107288074(CAPN2)

CTIG:

120307212(CAPN2)

TSR:	106549641(CAPN2)

PGUT:

117671750(CAPN2)

APRI:

131189346

PTEX:

113433240(CAPN2)

NSS:	113421716(CAPN2)

VKO:

123024724

PMUA:

114594632(CAPN2)

PRAF:

128411079 128418951(CAPN12)

ZVI:	118083208(CAPN2)

HCG:	128332565(CAPN12) 128350261

GJA:	107121636(CAPN2)

STOW:

125436868

EMC:	129330026(CAPN2) 129342991 129343726

XLA:	100127267(XB5844015.L) 108716477(capn8.5.L) 108717697(capn2.S) 399075(capn2.L)

XTR:	100379861(capn8.5) 100494922 100495980 448035(capn2)

NPR:	108786661 108787073(CAPN2)

RTEM:

120913392 120937867

ACAT:

141107755 141140828(CAPN2)

BBUF:

120977198 120999612(CAPN2)

BGAR:

122934009(CAPN2)

DTC:	141051967 141060116(CAPN2)

PWL:	138259658(CAPN12) 138295492

MUO:	115465480(CAPN2) 115481010

GSH:

117356530

DRE:	337730(capn8) 445091(capn2l) 541374(capn2a) 563053(capn2b)

SRX:	107722658 107722663 107722669 107724584 107734257 107755343 107755344 107755345 107755925

SANH:

107655329 107655374 107655376 107655378 107659324 107659328 107676365 107679193

SGH:	107549147 107565171 107565172 107565180 107566058 107569122 107571859 107577564 107586086

CCAR:

109046473(capn2l) 109047015(capn2b) 109057483 109062020 109070306 109076939 109100541(capn2a) 109111554

CAUA:

113039948 113039949 113039950 113039951 113065066 113065067 113065139 113065999 113092354 113112426

CGIB:

127942702 127943373 127943374 127956591 127970457 127988414 127988415 127988417 128025938

PTET:

122327143 122327282(capn2b) 122327288 122327868 122327869 122328220(capn2l) 122356702(capn2a)

LROH:

127153354(capn2b) 127159477(capn2l) 127159478 127159480 127175321(capn2a)

GRF:	141325177(capn2a) 141339041(capn8) 141339156 141339307(capn2b) 141339309(capn2l)

OMC:	131530830(capn8) 131531102(capn2l) 131531156 131551993(capn2a)

PPRM:

120479402(capn2a) 120495254(capn8) 120495255(capn2b) 120495447(capn2l)

RKG:	130083184(capn2a) 130103527(capn2l) 130103528(capn8)

MAMB:

125263723(capn2l) 125263724(capn8) 125263725 125263726 125263728 125263731(capn2b) 125276707(capn2a)

CIDE:

127520139 127520684 127521576 127521577 127524777

CERY:

137020441(capn2a) 137027817(capn2b) 137027820 137029203 137029204 137029205 137029208 137029209(capn2l)

MASI:

127412206 127413959 127438536 127438537 127438758 127438759

TROS:

130548323(capn2b) 130548655 130548689 130548690(capn2l) 130550965

TDW:	130429403(capn2b) 130429719 130429728 130430172(capn2l) 130431461(capn2a)

MANU:

129415666(capn2a) 129452865(capn2b) 129453082 129453423(capn2l) 129453424 129453425 129453426 129453427 129453428 129453429 129453430 129453432 129453433 129453436 129453438 141359517 141359519

PDAB:

135741001(capn2b) 135741004(capn2l) 135741005 135741006 135741008 135741009 135741010 135741131 135741132 135786616(capn2a)

IPU:	108259404 108259406 108259420

IFU:	128602567 128602877 128602878

PHYP:

113547581

SMEO:

124379814 124380107(capn8)

TFD:	113656951 113657035 113657038 113657047 113657081 113657082

TVC:	132838552 132839063(capn8)

CGAR:

128518526(capn8) 128518527 128518529 128518556

NGRA:

132874911(capn8) 132874912

TRN:	134308116 134314954(capn2a) 134334283 134334752 134334783

AMEX:

103024279(capn8) 103026146(capn2b) 107197131(capn2a)

CMAO:

118804562 118804578 118804588 118804589 118804595 118804599 118804628 118810905(capn2a)

EEE:	113577726 113581471 113581475

CHAR:

105896912 105910532 116219353 122129548 122129552 122129555 122132274

SPIL:

134061589 134061715(capn8) 134064510 134064515 134100748

TRU:	101064754 101076184 101076595

TFS:	130527429 130533559 130533560

TNG:	GSTEN00008927G001 GSTEN00030802G001 GSTEN00030804G001

LCO:	104918327 104918337 104934896 104934897 104934898

NCC:	104963449 104963450

TBEN:

117499311 117499313 117499673(capn8) 117499674

PGEO:

117441997 117459873 117459874

GACU:

117535626 117553009 117553016

EMAC:

134858433(capn8) 134858435 134862697 134862887 134863186

ELY:	117248470 117248471 117255565 117255600 117255876

EFO:	125885996(capn8) 125885997 125885998 125903649 125903650

PLEP:

121942132

SLUC:

116034148 116049140 116049141 116049166 116049190 116051494 116058023 116058030 116058374 116058375 116058502 118496042

ECRA:

117960009

ESP:

116704839

PFLV:

114556645 114556646 114556744 114557816 114557817 114557979 114557980 114563664 114563675 114566584 114572526

GAT:	120824594 120824596

PPUG:

119214540 119214541 119217601(capn2l) 119217602(capn2b)

AFB:	129092103 129092104 129095536 129095538 129095695

CLUM:

117729021 117743953 117743954

PSWI:

130191238 130191246 130206983 130207436

CANA:

137780110 137780111 137801993 137801994 137801995

CVE:	137866671 137866673 137866703(capn2l) 137882927 137882980

MSAM:

119899880(capn2l) 119899881(capn8) 119899882 119917657

SCHU:

122872848(capn8) 122872850 122884013

CUD:	121508149 121508172 121510754 121510929

SPUL:

138588182 138588979 138594452 138595237

LMIX:

132975131 132975132 132993622 132993654

ALAT:

119019705(capn2l) 119019734(capn8) 119019742(capn2b) 119033589 119034167

ASTR:

137600544 137600545 137600546 137603665 137603667

MZE:	101466554 101468385

ONL:	100706186 100709493 102083160

OAU:	116317696 116317707(capn8) 116317874(capn2l) 116318507

OLA:	101162579 101164919 101165172

OML:	112143530 112162134 112162135

CSAI:

133445752 133445763 133463976 133464168

XMA:	102227133 102234145

XCO:	114138434 114138435 114144102

XHE:	116713621 116713624 116720104

PRET:

103473154 103477212 103477213

PFOR:

103130124 103134145 103134146

PLAI:

106959257 106959267 106961194

PMEI:

106929428 106929429 106931777

GAF:	122829988 122842935 122842936

PPRL:

129356041 129359660(capn8) 129359661

CVG:	107082647 107082675 107102256 107102257

CTUL:

119785494 119785503 119797741 119797749

GMU:	124858622 124868214

NFU:	107375570 107393875

KMR:	108234077 108234124 108251143 108251144(capn2b)

ALIM:

106515918(capn11) 106536239

NWH:	119411348 119411359 119422313

AOCE:

111568891 111568893 111568895 111585239 111585240

EJA:	138195508 138195509 138208769 138209706

BFRE:

138616095 138616096 138634836 138634890

MCEP:

125004579(capn8) 125004580(capn2l) 125004581(capn2b) 125019146 125019147

CSEM:

103383278 103383281 103387544 103387545

POV:	109630641 109632323 109647469 109647544

SSEN:

122762072 122771004 122781965 122781966

SSOE:

131466793 131467261

HHIP:

117763891(capn2l) 117763907(capn8) 117763923 117775632 117776037 117776080

HSP:	118119322 118119434 118119503 118119505 118120876

PPLT:

128437470 128453113 128453899(capn8)

SMAU:

118283772 118283773 118312232 118312235(capn2l) 118312236(capn2b)

LCF:	108877132 108877133(capn8) 108882289

SDU:	111220638 111220639 111237555 111237556 111237557

SLAL:

111649592 111658604 111669521 111669522

XGL:	120796274 120796275 120800453(capn2l) 120800454(capn8) 120800455(capn2b)

HCQ:	109516162 109527604

SSCV:

125968492 125978751

SBIA:

133505698 133509944

PEE:	133403937 133410220 133410221

PTAO:

133476952 133485740 133485741

BPEC:

110156974 110156975 110166012

ENY:	140350952 140351565 140365137 140375899

MALB:

109960723 109971792 109971799 109971826

BSPL:

114857498 114857509(capn2b) 114870532 114870586 114870587

SJO:	128357669 128373143 128373144 128375865 128378195

SSCO:

133976931 133992285 133992369 134000275

GMH:	115539934 115539935 115559787

SASA:

106565007 106565009 106578800 106578801 106584767 106589959 106589985

STRU:

115155631 115155640 115201755 115201756 115208449 115208455

OTW:	112223208 112223950 112236240 112266430(capn8) 112266436

OMY:	100135837 100136224(capn2) 110491224 110491235 110493820(capn8)

OGO:	124002438 124003579(capn8) 124039071 124048054 124048056

ONE:	115107157 115122462 115126231 115142697 115142699

OKI:	109879240 109880977 109890699 109890707 116355398

OKE:	118376416 118379311 118379869 118389064 118389067(capn8)

OMM:	135505262 135505264 135539481

OCLA:

139367880 139367881 139369375 139370607 139377011

SALP:

111960924 111960936

SNH:	120022367(capn8) 120029335 120045314 120045401 120064858

CCLU:

121555339 121572329 121572330(capn8) 121580572 121580650 123484545

ELS:	105028009 105028010 105031125

OEP:	134008523 134008524(capn8) 134022287

SFM:	108927097 108927098 108927128 108933713 108933714

PKI:	111835677 111841738 111852308 111852340 111852381 111852382

AANG:

118212877 118212878 118217793(capn2a) 118218445 118218446(capn8) 118220682 118220959

AROT:

135239032 135245073(capn8) 135245074(capn2a) 135245220 135245571 135249567

CCOG:

133115141(capn8) 133116027 133116505 133118029 133118365 133118366

LOC:	102683469 102686084 102686287

PSPA:

121315710 121315712 121316792 121316797 121316798

ARUT:

117402852 117403231 117403377 117410845 117410846 117411201

PSEX:

120516279(capn8) 120516295(capn2l) 120516636 120516907

LCM:	102347065(CAPN2)

CMK:

103177931

HOC:	132819701 132830514

LERI:

129699624

HAKA:

140730337 140733023

PMRN:

116943157

AGIF:

122847603 122849323

TPAL:

117639677

PVUL:

126817811

GAE:	121379756 121386152 121386313

HRF:	124115873 124136697 124145344

HRJ:	124274220 124291720

HASI:

137256212 137256542 137276711

HCRA:

139459127 139464408 139470348

CVN:	111110708 111127234

OED:

125666590

AIRR:

138329990

MMER:

123566327

RPHI:

132718623 132745282

DPOL:

127877971

MAEA:

128228716

OSN:

115209388

LJP:

135482163

» show all

Reference

1 [PMID:10639123]

Authors

Strobl S, Fernandez-Catalan C, Braun M, Huber R, Masumoto H, Nakagawa K, Irie A, Sorimachi H, Bourenkow G, Bartunik H, Suzuki K, Bode W.

Title

The crystal structure of calcium-free human m-calpain suggests an electrostatic switch mechanism for activation by calcium.

Journal

Proc Natl Acad Sci U S A 97:588-92 (2000)
DOI:10.1073/pnas.97.2.588

Sequence

[hsa:824]

Reference

2 [PMID:12014988]

Authors

Dutt P, Spriggs CN, Davies PL, Jia Z, Elce JS

Title

Origins of the difference in Ca2+ requirement for activation of mu- and m-calpain.

Journal

Biochem J 367:263-9 (2002)
DOI:10.1042/BJ20020485

Other DBs

ExplorEnz - The Enzyme Database:

3.4.22.53

IUBMB Enzyme Nomenclature:

3.4.22.53

ExPASy - ENZYME nomenclature database:

3.4.22.53

BRENDA, the Enzyme Database:

3.4.22.53

CAS:	702693-80-9

All links

Gene (772)   
   MGENES (225)   
   RefGene (547)   
Protein sequence (987)   
   UniProt (928)   
   SWISS-PROT (11)   
   RefSeq(pep) (27)   
   PDBSTR (18)   
   PMD (3)   
DNA sequence (34)   
   RefSeq(nuc) (30)   
   GenBank (4)   
3D Structure (7)   
   PDB (7)   
Protein domain (13)   
   InterPro (12)   
   PROSITE(DOC) (1)   
All databases (1813)   

Download RDF

DBGET integrated database retrieval system