KEGG ENZYME: 3.4.22.53

ENZYME: 3.4.22.53

Entry

EC 3.4.22.53 Enzyme

Name

calpain-2;
calcium-activated neutral protease II;
m-calpain;
milli-calpain

Class

Hydrolases;
Acting on peptide bonds (peptidases);
Cysteine endopeptidases

Reaction(IUBMB)

Broad endopeptidase specificity

Comment

Type example of peptidase family C2. Requires Ca2+ at millimolar concentrations for activity. Cytosolic in animal cells. The active enzyme molecule is a heterodimer in which the large subunit contains the peptidase unit, and the small subunit is also a component of EC 3.4.22.52, calpain-1.

History

EC 3.4.22.53 created 1981 as EC 3.4.22.17, transferred 2003 to EC 3.4.22.53

Orthology

K03853

calpain-2

Genes

HSA:	824(CAPN2)

PTR:	457765(CAPN2)

PPS:	100988890(CAPN2)

GGO:	101149206(CAPN2)

PON:	100445754(CAPN2)

NLE:	100589114(CAPN2)

MCC:	703047(CAPN2)

MCF:	102122692(CAPN2)

CSAB:

103230023(CAPN2)

CATY:

105576962(CAPN2)

PANU:

101022030(CAPN2)

RRO:	104677285(CAPN2)

RBB:	108528248(CAPN2)

TFN:	117075199(CAPN2)

PTEH:

111537094(CAPN2)

CJC:	100405215(CAPN2)

SBQ:	101035587(CAPN2)

MMUR:

105863767

MMU:	12334(Capn2)

MCAL:

110296563(Capn2)

MPAH:

110321506(Capn2)

RNO:	29154(Capn2)

MCOC:

116068107(Capn2)

MUN:	110546658(Capn2)

CGE:	100771239(Capn2)

PLEU:

114684418(Capn2)

NGI:	103726266(Capn2)

HGL:	101720177(Capn2)

CCAN:

109677038(Capn2)

OCU:	100009092(CAPN2)

TUP:	102481316(CAPN2)

CFA:	480118(CAPN2)

VVP:	112923580(CAPN2)

VLG:	121485762(CAPN2)

AML:	100476150(CAPN2)

UMR:	103669131(CAPN2)

UAH:	113262899(CAPN2)

ORO:	101364547(CAPN2)

ELK:

111150685

MPUF:

101675244(CAPN2)

EJU:	114215532(CAPN2)

MLX:	118012992(CAPN2)

FCA:	101083231(CAPN2)

PYU:	121015188(CAPN2)

PBG:	122475955(CAPN2)

PPAD:

109258888(CAPN2)

AJU:	106983173(CAPN2)

HHV:	120234148(CAPN2)

BTA:	281662(CAPN2)

BOM:	102276298(CAPN2)

BIU:	109570370(CAPN2)

BBUB:

102415122(CAPN2)

CHX:	102181227(CAPN2)

OAS:	100127211(CAPN2)

ODA:	120880362(CAPN2)

CCAD:

122452395(CAPN2)

SSC:	397393(CAPN2)

CFR:	102512386(CAPN2)

CBAI:

105083948(CAPN2)

CDK:	105099835(CAPN2)

BACU:

103007207(CAPN2) 103019749

LVE:	103080579(CAPN2)

OOR:	101270285(CAPN2)

DLE:	111177507(CAPN2)

PCAD:

102976041(CAPN2)

ECB:	100055184(CAPN2)

EPZ:	103563904(CAPN2)

EAI:	106829535(CAPN2)

MYB:	102240228(CAPN2)

MYD:	102769788(CAPN2)

MMYO:

118678942(CAPN2)

MNA:	107543584(CAPN2)

HAI:	109381194(CAPN2)

DRO:

112318759

SHON:

119003139

AJM:	119036522(CAPN2)

MMF:	118640556(CAPN2)

PALE:

102897695(CAPN2)

PGIG:

120619310(CAPN2)

RAY:

107498969

MJV:	108400832(CAPN2)

TOD:	119244503(CAPN2)

LAV:	100663804(CAPN2)

TMU:

101341399

MDO:	100018672(CAPN2)

SHR:	100922649(CAPN2)

PCW:	110205536(CAPN2)

OAA:	100081577(CAPN2)

GGA:	395963(CAPN2)

PCOC:

116230900(CAPN2)

MGP:	100546180(CAPN2)

CJO:	107311033(CAPN2)

NMEL:

110395702

APLA:

101799042

ACYG:

106031621(CAPN2)

TGU:

100225408

LSR:

110483833

SCAN:

103819275(CAPN2)

PMOA:

120505963(CAPN2)

GFR:	102045030(CAPN2)

FAB:	101809391(CAPN2)

PHI:	102111446(CAPN2)

PMAJ:

107202529(CAPN2)

CCAE:

111926249(CAPN2)

CCW:	104685555(CAPN2)

ETL:	114073709(CAPN2)

FPG:	101919959(CAPN2)

FCH:	102048431(CAPN2)

CLV:

102084552

EGZ:	104124542(CAPN2)

NNI:	104019299(CAPN2)

ACUN:

113477955(CAPN2)

PADL:

103925056(CAPN2)

AAM:	106482724(CAPN2)

AROW:

112963405

NPD:	112954803(CAPN2)

DNE:	112980095(CAPN2)

ASN:	102373508(CAPN2)

AMJ:	102560432(CAPN2)

CPOO:

109319765(CAPN2)

GGN:	109296890(CAPN2)

PSS:	102458713(CAPN2)

CMY:	102937008(CAPN2)

CPIC:

101940996(CAPN2)

TST:	117874491(CAPN2) 117888892

CABI:

116825598(CAPN2)

ACS:	100561808(capn2)

PVT:	110075406(CAPN2)

PBI:

103056946

PMUR:

107288074(CAPN2)

TSR:	106549641(CAPN2)

PGUT:

117671750

PMUA:

114594632(CAPN2)

ZVI:	118083208(CAPN2)

GJA:	107121636(CAPN2)

XLA:	100127267(XB5844015.L) 108716477(capn8.5.L) 108717697(capn2.S) 399075(capn2.L)

XTR:	100379861(capn8.5) 100494922 100495980 448035(capn2)

NPR:	108786661 108787073(CAPN2)

DRE:	337730(capn8) 445091(capn2l) 541374(capn2a) 563053(capn2b)

SRX:	107722658 107722663 107722669 107724584 107734257 107755343 107755344 107755345 107755925

SANH:

107655329 107655374 107655376 107655378 107659324 107659328 107676365 107679193

SGH:	107549147 107565171 107565172 107565180 107566058 107569122 107571859 107577564 107586086

CCAR:

109046872 109046967 109046972 109047015 109070306 109076939 109081032 109100541 109106274

CAUA:

113039948 113039949 113039950 113039951 113065066 113065067 113065139 113065999 113092354 113112426

IPU:	108259404 108259406(CAPN2) 108259420

PHYP:

113547581

AMEX:

103024279 103026146 107197131

EEE:	113577726 113581471 113581475

TRU:	101064754 101076184 101076595

TNG:	GSTEN00008927G001 GSTEN00030802G001 GSTEN00030804G001

LCO:	104918327 104918337 104934896 104934897 104934898

NCC:	104963449 104963450

ELY:	117248470 117248471 117255565 117255600 117255876

PLEP:

121942132

SLUC:

116034148 116049140 116049141 116049166 116049190 116051494 116058023 116058030 116058374 116058375 116058502 118496042

ECRA:

117960009

PFLV:

114556645 114556646 114556744 114557816 114557817 114557979 114557980 114563664 114563675 114566584 114572526

GAT:	120824594 120824596

MSAM:

119899880(capn2l) 119899881(capn8) 119899882 119917657

CUD:	121508149 121508172 121510754 121510929

MZE:	101466554 101468385

ONL:	100706186 100709493 102083160

OAU:	116317696 116317707(capn8) 116317874(capn2l) 116318507

OLA:	101162579 101164919 101165172

OML:	112143530 112162134 112162135

XMA:	102227133 102234145

XCO:	114138434 114138435 114144102

XHE:	116713621 116713624 116720104

PRET:

103473154 103477212 103477213

CVG:	107082647 107082675 107102256 107102257

CTUL:

119785494 119785503 119797741 119797749

NFU:	107375570 107393875

KMR:	108234077 108234124 108251143 108251144(capn2b)

ALIM:

106515918(capn11) 106536239

AOCE:

111568891 111568893 111568895 111585239

CSEM:

103383278 103383281 103387544 103387545

POV:	109630641 109632323 109647469 109647544

LCF:	108877132 108877133 108882289

SDU:	111220638 111220639 111237555 111237556 111237557

SLAL:

111649592 111658604 111669521 111669522

XGL:	120796274 120796275 120800453(capn2l) 120800454(capn8) 120800455(capn2b)

HCQ:	109516162 109527604

BPEC:

110156974 110156975 110166012

MALB:

109960723 109971792 109971799 109971826

SASA:

106565007 106565009 106578800 106578801 106584767 106589959 106589985

OTW:	112223208 112266430 112266436

OMY:	100135837 100136224(capn2) 110491224 110491235 110493820(capn8)

SALP:

111960924 111960936

SNH:	120022367(capn8) 120029335 120045314 120045401 120064858

ELS:	105028009 105028010 105031125

SFM:	108927097 108927098 108927128 108933713 108933714

PKI:	111835677 111841738 111852308 111852340 111852381 111852382

AANG:

118212877 118212878 118217793(capn2a) 118218445 118218446(capn8) 118220682 118220959

LOC:	102683469 102686084 102686287

PSPA:

121315710 121315712 121316792 121316797 121316798

ARUT:

117402852 117403231 117403377 117410845 117410846 117411201

LCM:	102347065(CAPN2)

CMK:

103177931

RTP:

109927474

GAE:	121379756 121386152 121386313

» show all

Reference

1 [PMID:10639123]

Authors

Strobl S, Fernandez-Catalan C, Braun M, Huber R, Masumoto H, Nakagawa K, Irie A, Sorimachi H, Bourenkow G, Bartunik H, Suzuki K, Bode W.

Title

The crystal structure of calcium-free human m-calpain suggests an electrostatic switch mechanism for activation by calcium.

Journal

Proc Natl Acad Sci U S A 97:588-92 (2000)
DOI:10.1073/pnas.97.2.588

Sequence

[hsa:824]

Reference

2 [PMID:12014988]

Authors

Dutt P, Spriggs CN, Davies PL, Jia Z, Elce JS

Title

Origins of the difference in Ca2+ requirement for activation of mu- and m-calpain.

Journal

Biochem J 367:263-9 (2002)
DOI:10.1042/BJ20020485

Other DBs

ExplorEnz - The Enzyme Database:

3.4.22.53

IUBMB Enzyme Nomenclature:

3.4.22.53

ExPASy - ENZYME nomenclature database:

3.4.22.53

BRENDA, the Enzyme Database:

3.4.22.53

CAS:	702693-80-9

DBGET integrated database retrieval system