Acting on peptide bonds (peptidases);
Strict requirement for Asp at the P1 position. It has a preferred cleavage sequence of Tyr-Val-Ala-Asp! but also cleaves at Asp-Glu-Val-Asp!
This enzyme is part of the family of inflammatory caspases, which also includes caspase-1 (EC 18.104.22.168) and caspase-4 (EC 22.214.171.124) in humans and caspase-11 (EC 126.96.36.199), caspase-12, caspase-13 and caspase-14 in mice. Contains a caspase-recruitment domain (CARD) in its N-terminal prodomain, which plays a role in procaspase activation [3,5,6]. The enzyme is able to cleave itself and the p30 caspase-1 precursor, but is very inefficient at generating mature interleukin-1beta (IL-1beta) from pro-IL-1beta [1,4]. Both this enzyme and caspase-4 can cleave pro-caspase-3 to release the small subunit (p12) but not the large subunit (p17) . Unlike caspase-4, this enzyme can be induced by lipopolysaccharide . Belongs in peptidase family C14.