KEGG   ENZYME: 3.4.23.49
Entry
EC 3.4.23.49                Enzyme                                 
Name
omptin;
protease VII;
protease A;
gene ompT proteins;
ompT protease;
protein a;
Pla;
OmpT
Class
Hydrolases;
Acting on peptide bonds (peptidases);
Aspartic endopeptidases
Reaction(IUBMB)
Has a virtual requirement for Arg in the P1 position and a slightly less stringent preference for this residue in the P1' position, which can also contain Lys, Gly or Val.
Comment
A product of the ompT gene of Escherichia coli, and associated with the outer membrane. Omptin shows a preference for cleavage between consecutive basic amino acids, but is capable of cleavage when P1' is a non-basic residue [5,7]. Belongs in peptidase family A26.
History
EC 3.4.23.49 created 1993 as EC 3.4.21.87, transferred 2006 to EC 3.4.23.49
Orthology
K01355  omptin
Genes
ECOb0565(ompT)
ECJJW0554(ompT)
ECDECDH10B_0523(ompT) ECDH10B_0631(ompT)
EBWBWG_0436(ompT)
ECEZ1931
ECSECs_1663(ompT)
ECFECH74115_1653
ETWECSP_1567(ompT)
ELXCDCO157_1594
EOIECO111_0586
EOJECO26_0631
EOHECO103_0566
ECOOECRM13514_0582
ECGE2348C_0466(ompT)
EOKG2583_0717(ompT)
ELRECO55CA74_03500
ECPECP_0597
ECOSEC958_0673(ompT)
ECVAPECO1_1482(ompT) APECO1_546
ECOAAPECO78_06360
ECMEcSMS35_A0099(ompT)
ECYECSE_0621
EUMECUMN_0637(ompT)
ECTECIAI39_0539(ompT)
EOCCE10_0562(ompT)
EBRECB_00519(ompT)
ECIUTI89_C0566(ompT)
EIHECOK1_0571(ompT) ECOK1_0575(ompT)
ECZECS88_0597(ompT) ECS88_0602(ompT) ECS88_p0155(ompT)
ECCc0652(ompT)
ELNNRG857_02540 NRG857_30283(ompT1)
ESEECSF_0496
EABECABU_c06150
EDHEcDH1_3064
EDJECDH1ME8569_0533(ompT)
ELUUM146_14685
ELCi14_0626(ompT)
ELDi02_0626(ompT)
ELFLF82_1565(ompT)
ECOIECOPMV1_00578(ompT_1) ECOPMV1_00582(ompT_2)
ECOJP423_02720
EALEAKF1_ch0285 EAKF1_ch0900
ESZFEM44_05105 FEM44_13015
SESSARI_01746
ENLA3UG_21740
ENFAKI40_2865
CROROD_20151(ompT)
EBTEBL_c18290
KCOBWI95_15415
KOTEH164_12575
KOBHF650_10520
YRUBD65_376(ompT)
EAMEGXP68_13960
EAMEAMY_0249(ompT1) EAMY_2844(ompT3) EAMY_2848(ompT5)
EAYEAM_0238 EAM_0740 EAM_0742
EPYEpC_02590(ompT)
EPREPYR_00269(ompT)
ERJEJP617_13540(ompT)
PFQQQ39_09680
PCGAXG94_20795
PMEDE3Z27_05160
ABERBSR55_19170
MESLKKZ03_21340
PPRFDPRO_2449
SIXBSY16_2715
EMXFKV68_24320
AGRAGROH133_05297
ATFAch5_10520
AGTEYD00_04005
RELREMIM1_PE00480
RIRBN877_I1129
RPUSCFBP5875_04415
RHVBA939_00570
ROYG3A56_05140
DEIC4375_08320
DEAFPZ08_15605
 » show all
Reference
1  [PMID:3278297]
  Authors
Grodberg J, Lundrigan MD, Toledo DL, Mangel WF, Dunn JJ
  Title
Complete nucleotide sequence and deduced amino acid sequence of the ompT gene of Escherichia coli K-12.
  Journal
Nucleic Acids Res 16:1209 (1988)
DOI:10.1093/nar/16.3.1209
  Sequence
[eco:b0565]
Reference
2  [PMID:3056908]
  Authors
Sugimura K, Nishihara T.
  Title
Purification, characterization, and primary structure of Escherichia coli protease VII with specificity for paired basic residues: identity of protease VII and OmpT.
  Journal
J Bacteriol 170:5625-32 (1988)
DOI:10.1128/JB.170.12.5625-5632.1988
  Sequence
[eco:b0565]
Reference
3  [PMID:1603076]
  Authors
Hanke C, Hess J, Schumacher G, Goebel W.
  Title
Processing by OmpT of fusion proteins carrying the HlyA transport signal during secretion by the Escherichia coli hemolysin transport system.
  Journal
Mol Gen Genet 233:42-8 (1992)
DOI:10.1007/bf00587559
Reference
4
  Authors
Dekker, N.
  Title
Omptin.
  Journal
In: Barrett, A.J., Rawlings, N.D. and Woessner, J.F. (Eds.), Handbook of Proteolytic Enzymes, 2nd ed., Elsevier, London, 2004, p. 212-216.
Reference
5  [PMID:11566868]
  Authors
Vandeputte-Rutten L, Kramer RA, Kroon J, Dekker N, Egmond MR, Gros P.
  Title
Crystal structure of the outer membrane protease OmpT from Escherichia coli suggests a novel catalytic site.
  Journal
EMBO J 20:5033-9 (2001)
DOI:10.1093/emboj/20.18.5033
  Sequence
[eco:b0565]
Reference
6  [PMID:11576541]
  Authors
Kramer RA, Vandeputte-Rutten L, de Roon GJ, Gros P, Dekker N, Egmond MR.
  Title
Identification of essential acidic residues of outer membrane protease OmpT supports a novel active site.
  Journal
FEBS Lett 505:426-30 (2001)
DOI:10.1016/S0014-5793(01)02863-0
  Sequence
[eco:b0565]
Reference
7  [PMID:15317797]
  Authors
McCarter JD, Stephens D, Shoemaker K, Rosenberg S, Kirsch JF, Georgiou G.
  Title
Substrate specificity of the Escherichia coli outer membrane protease OmpT.
  Journal
J Bacteriol 186:5919-25 (2004)
DOI:10.1128/JB.186.17.5919-5925.2004
Other DBs
ExplorEnz - The Enzyme Database: 3.4.23.49
IUBMB Enzyme Nomenclature: 3.4.23.49
ExPASy - ENZYME nomenclature database: 3.4.23.49
BRENDA, the Enzyme Database: 3.4.23.49
CAS: 150770-86-8

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