KEGG   ENZYME: 3.4.24.21
Entry
EC 3.4.24.21                Enzyme                                 

Name
astacin;
Astacus proteinase;
crayfish small-molecule proteinase
Class
Hydrolases;
Acting on peptide bonds (peptidases);
Metalloendopeptidases
Reaction(IUBMB)
Hydrolysis of peptide bonds in substrates containing five or more amino acids, preferentially with Ala in P1', and Pro in P2'
Comment
A 22.6 kDa digestive endopeptidase from the cardia of the crayfish Astacus fluviatilis. Type example of peptidase family M12.
History
EC 3.4.24.21 created 1972 as EC 3.4.99.6, transferred 1992 to EC 3.4.24.21
Orthology
K08076  astacin
Genes
CEL: CELE_4R79.1(nas-6) CELE_C07D10.4(nas-7) CELE_C24F3.3(nas-12) CELE_C26C6.3(nas-36) CELE_C34D4.9(nas-8) CELE_C37H5.9(nas-9) CELE_F09E8.6(nas-14) CELE_F38E9.2(nas-39) CELE_F40E10.1(hch-1) CELE_F42A10.8(nas-28) CELE_F45G2.1(nas-1) CELE_F46C5.3(nas-25) CELE_F57C12.1(nas-38) CELE_F58A6.4(nas-29) CELE_K03B8.1(nas-16) CELE_K03B8.2(nas-17) CELE_K03B8.3(nas-18) CELE_K04E7.3(nas-33) CELE_K06A4.1(nas-3) CELE_K09C8.3(nas-10) CELE_T02B11.7(nas-32) CELE_T04G9.2(nas-15) CELE_T11F9.3(nas-20) CELE_T23F4.4(nas-27) CELE_T23H4.3(nas-5)
CBR: CBG00430(Cbr-nas-6)
TSP: Tsp_00923
NVE: 5512699
EPA: 110244690
AMIL: 114957426
SPIS: 111326758
DGT: 114525285
Reference
1  [PMID:7041692]
  Authors
Krauhs E, Dorsam H, Little M, Zwilling R, Ponstingl H.
  Title
A protease from Astacus fluviatilis as an aid in protein sequencing.
  Journal
Anal Biochem 119:153-7 (1982)
DOI:10.1016/0003-2697(82)90679-0
Reference
2  [PMID:3548817]
  Authors
Titani K, Torff HJ, Hormel S, Kumar S, Walsh KA, Rodl J, Neurath H, Zwilling R.
  Title
Amino acid sequence of a unique protease from the crayfish Astacus fluviatilis.
  Journal
Biochemistry 26:222-6 (1987)
DOI:10.1021/bi00375a029
  Sequence
Reference
3
  Authors
Stocker, W., Wolz, R.L., Zwilling, R., Strydom, D.J. and Auld, D.S.
  Title
Astacus protease, a zinc metalloenzyme.
  Journal
Biochemistry 27:5026-5032 (1988)
Reference
4  [PMID:2261483]
  Authors
Stocker W, Ng M, Auld DS
  Title
Fluorescent oligopeptide substrates for kinetic characterization of the specificity of Astacus protease.
  Journal
Biochemistry 29:10418-25 (1990)
DOI:10.1021/bi00497a018
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 3.4.24.21
IUBMB Enzyme Nomenclature: 3.4.24.21
ExPASy - ENZYME nomenclature database: 3.4.24.21
BRENDA, the Enzyme Database: 3.4.24.21
CAS: 143179-21-9

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Gene (34)   
   KEGG ORTHOLOGY (1)   
   KEGG GENES (33)   
Protein sequence (58)   
   UniProt (22)   
   SWISS-PROT (4)   
   RefSeq(pep) (12)   
   PDBSTR (18)   
   PMD (2)   
DNA sequence (52)   
   RefSeq(nuc) (17)   
   GenBank (22)   
   EMBL (13)   
3D Structure (11)   
   PDB (11)   
Protein domain (8)   
   InterPro (7)   
   PROSITE(DOC) (1)   
All databases (163)   

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