KEGG ENZYME: 3.4.24.25

ENZYME: 3.4.24.25

Entry

EC 3.4.24.25                Enzyme

Name

vibriolysin;
Aeromonas proteolytica neutral proteinase;
aeromonolysin

Class

Hydrolases;
Acting on peptide bonds (peptidases);
Metalloendopeptidases

Reaction(IUBMB)

Preferential cleavage of bonds with bulky hydrophobic groups in P2 and P1'. Phe at P1' is the most favoured residue, which distinguished this enzyme from thermolysin

Comment

Thermostable enzyme from Vibrio proteolyticus (formerly Aeromonas proteolytica). Specificity related to, but distinct from, those of thermolysin and bacillolysin [1]. A zinc metallopeptidase in family M4 (thermolysin family). Formerly included in EC 3.4.24.4

History

EC 3.4.24.25 created 1972 as EC 3.4.24.4, part transferred 1992 to EC 3.4.24.25, modified 1997

Orthology

K08604

vibriolysin

Genes

XSA:	SB85_15700

XTH:	G4Q83_21425

XAG:	HEP73_04011(nprV)

LAB:	LA76x_2473(nprV) LA76x_2475

LAQ:	GLA29479_1171(empA) GLA29479_1174(hap)

LCP:	LC55x_2887(nprV) LC55x_2889 LC55x_2891

LGU:	LG3211_2739 LG3211_2741(nprV) LG3211_4445

LEZ:	GLE_2741(nprV) GLE_2743(nprV)

LEM:	LEN_2283 LEN_2284

LMB:	C9I47_0200

LYT:	DWG18_10025

LSX:	H8B22_08015

LHX:	LYSHEL_12180

LCAS:

LYSCAS_12180

LLZ:	LYB30171_01014

LYA:	RDV84_18675 RDV84_18680

THES:

FHQ07_05060

THEH:

G7079_11500

TCN:	H9L16_08945

TBV:	H9L17_11900

FSG:	LQ771_02990 LQ771_09765 LQ771_13930

FED:	LQ772_03675 LQ772_11020 LQ772_15560

RHD:	R2APBS1_0936

RGL:	CS053_13835

RTH:	LRK53_06130 LRK53_12690

DTX:	ATSB10_19710

LRZ:	BJI69_12675

LPY:	FIV34_05775

LANH:

KR767_01775

LFL:	IM816_04480

LAEG:

L2Y94_16255

LAEO:

L2Y97_05845

LAES:

L2Y96_06360

XBA:	C7S18_19035

RBD:	ALSL_0921 ALSL_2568

VCH:

VC_A0865

VCF:	IR04_03775

VCS:

MS6_A0903

VCE:	Vch1786_II0554(hap)

VCQ:	EN18_02630

VCJ:	VCD_000466

VCI:

O3Y_17553

VCO:	VC0395_0371(hap)

VCR:	VC395_A0889(hap)

VCM:	VCM66_A0824(hap)

VCL:	VCLMA_B0638

VCX:	VAA049_2123(hap)

VCZ:	VAB027_324(hap)

VVU:

VV2_0974

VVY:

VVA1465

VVM:	VVMO6_04367

VVL:	VV93_v1c43660(lasB)

VPA:

VPA0755

VPB:	VPBB_A0695

VPF:	M634_21715

VPH:	VPUCM_20654

VHA:	VIBHAR_02509 VIBHAR_05515

VCA:	M892_00890 M892_23945

VEX:	VEA_000274

VDB:	AL552_06510

VOW:	A9237_15790 A9237_19765

VRO:	BSZ04_00415

VSP:	VS_1267(vsm)

VFU:	vfu_B00520

VAN:

VAA_01306

LAG:	N175_16430

VAU:	VANGNB10_cII0919c(empA)

VCY:	IX92_04640 IX92_17695 IX92_22445

VCT:	JV59_17150 JV59_21375 JV59_34105

VTU:	IX91_16525

VEU:	IXK98_02965

VSI:	MTO69_17135

VFL:	AL536_05555

VMI:	AL543_06040

VGA:	BSQ33_18790

VQI:	CCZ37_14020 CCZ37_14710

VCC:	FAZ90_06635 FAZ90_18460

VAQ:	FIV01_09435(hap2) FIV01_17270(nprV1) FIV01_19305(nprV2)

VSR:	Vspart_04278(nprV)

VKA:	BTD91_00265 BTD91_17540

VNV:	IF132_01670

VGI:	MID13_09035 MID13_15870

VSL:	LTQ54_15765 LTQ54_16510 LTQ54_21280

VPL:	SA104470976_03599(hap)

VTI:	CEQ48_01775

VCRA:

IS519_00895 IS519_16885

VNP:	KW548_13075 KW548_18330 KW548_21875

VLE:	ISX51_08050 ISX51_19380 ISX51_22605

GKD:	K6Q96_22635 K6Q96_23505

SALY:

E8E00_07740 E8E00_14545

SKS:	FCN78_05640 FCN78_13840

SCOT:

HBA18_05895 HBA18_14490

SPRO:

N7E60_06120 N7E60_16375

PAGR:

E2H98_08005

SDN:

Sden_3015

SAZ:	Sama_0428 Sama_1201

SBL:

Sbal_3874

SBM:	Shew185_0439

SBN:	Sbal195_0456

SBP:	Sbal223_0465

SBT:	Sbal678_0463

SBS:	Sbal117_4032

SBB:	Sbal175_0534

SHE:	Shewmr4_2515

SHM:	Shewmr7_2583

SHN:	Shewana3_2681

SWD:	Swoo_0557 Swoo_3129

SVO:

SVI_2120

SHF:	CEQ32_07390

SPSW:

Sps_01606 Sps_02676 Sps_03413 Sps_03524

SBJ:	CF168_07495

SHEW:

CKQ84_10610 CKQ84_19125

SKH:	STH12_00210(hap_1) STH12_00763(lasB) STH12_04148(hap_2)

SLIT:

JQC75_02050 JQC75_05895

SDK:	KHX94_06455

SPSH:

FM037_17665

SYK:	KDN34_02045

SCYP:

JYB88_01960 JYB88_06140

SAVI:

JYB87_16680

SSEM:

JYB85_02135 JYB85_06435

SACH:

K0H61_11395

SZH:	K0H63_02290 K0H63_06520

SMAY:

K0H60_13535

SOG:	RA178_02405

THAT:

H3N35_00170 H3N35_00175

TACT:

SG35_000160 SG35_000165 SG35_029565

TVD:	SG34_029320 SG34_029325 SG34_032645

PSM:

PSM_B0576

PIA:	PI2015_3586

PPHE:

PP2015_3255 PP2015_3842

PBW:	D172_014080 D172_014110 D172_019810

PRR:	AT705_01970 AT705_21455 AT705_23865 AT705_23895 AT705_24095

PLZ:	S4054249_19905 S4054249_21035 S4054249_21745

PALN:

B0W48_01265

PPIS:

B1L02_19675 B1L02_23455 B1L02_23510

PEA:	PESP_b0778

PTU:	PTUN_a1933 PTUN_b0532

PTD:	PTET_b0708

PSEN:

PNC201_18505(empA) PNC201_22380(hap)

PDJ:	D0907_17045

PAGA:

PAGA_b0882

PCAR:

PC2016_3245

PXI:	J5O05_00755 J5O05_21200

PVB:	J5X90_00010 J5X90_18620 J5X90_20530 J5X90_22665 J5X90_22945 J5X90_22950

PSAZ:

PA25_02030 PA25_34250

PFLI:

CTT31_18695 CTT31_20005

AGZ:	M0C34_05585

MVS:	MVIS_1898(empA) MVIS_2920(empA)

MYA:	MORIYA_1508(nprV) MORIYA_2361(nprV)

MMAA:

FR932_02520

MOQ:	HWV03_05935 HWV03_07230

TTU:	TERTU_3351

SAGA:

M5M_18570

MII:	BTJ40_13250 BTJ40_13270

MICT:

FIU95_09120(nprV)

MHYD:

GTQ55_10450

MICZ:

GL2_38190

MVB:	MJO52_11575

LPN:

lpg0019

LPH:

LPV_0023

LPO:

LPO_0020

LPU:	LPE509_03226

LPM:

LP6_0020

LPF:

lpl0020

LPP:

lpp0019

LPC:

LPC_0020

LPA:

lpa_00026

LPE:

lp12_0019

LLO:

LLO_0045

LFA:

LFA_0023

LSH:	CAB17_00130 CAB17_04525

LGT:	E4T54_04155

LCJ:	NCTC11976_00699(hap)

LWA:	SAMEA4504053_0021(hap) SAMEA4504053_0892(nprV)

LSS:	NCTC12082_01281(hap)

LANT:

TUM19329_03270

TMC:	LMI_0117 LMI_1381

PSAL:

PSLF89_34

TIG:

THII_0643

CYS:	NYP54_08535

RHH:	E0Z06_02880

WOC:	BA177_08085

HCH:

HCH_02479

HAHE:

ENC22_11265 ENC22_12285

HAHH:

O5O45_06305

MME:	Marme_2860

GSN:	YC6258_00785 YC6258_04970

RFO:	REIFOR_01115 REIFOR_01121 REIFOR_02033

PLEI:

Q9312_13695 Q9312_17865

AHA:

AHA_0851

AHY:	AHML_04340

AHD:	AI20_14990

AHR:	V428_04535

AHP:	V429_04535

AHJ:	V469_18780

AHH:	RY45_04760

AHI:	VU14_18245

AAJ:	BOQ57_04220

AEO:	O23A_p4509

AVR:

B565_3414

AVO:	AMS64_19910

AMED:

B224_0573

ASR:	WL1483_2962(nprV)

ADH:	CK627_08250

ACAV:

VI35_03960

AEA:	C2U39_04290

AES:	C2U30_09950

AEL:	NCTC12917_00828(nprV)

AALL:

I6G90_19165

AJD:	I6H43_07255

ARIV:

KYK33_16480

ASAA:

KXJ75_04455

AEJ:	E5E97_08830

ABES:

IU367_17485

KKO:

Kkor_0311

KGE:

TQ33_2045

KPD:	CW740_01305

GKN:	PVT67_03015 PVT67_09445

IFL:	C1H71_01435

CARI:

FNU76_01840 FNU76_12870

CKS:	H9L41_00435

KIA:	G8A07_16855

RDP:	RD2015_2455

ROI:	N4261_15900

MIU:	ABE85_14380

JAB:	VN23_08790

CPRA:

CPter91_2011

UPV:	EJN92_01400

PAQA:

K9V56_005565

HOE:	IMCC20628_04027

BBAN:

J4G43_043050

DALK:

DSCA_47230

MXA:	MXAN_6106(fibA)

MSD:	MYSTI_02756 MYSTI_03758 MYSTI_06723

MYM:	A176_000783

MFB:	MFUL124B02_15035 MFUL124B02_35290 MFUL124B02_41125

CCX:	COCOR_00680(fibA1) COCOR_05260(empA) COCOR_05722(nprV) COCOR_06634(fibA)

MFU:	LILAB_15735

MMAS:

MYMAC_005892

AGE:	AA314_01740 AA314_04229 AA314_05554 AA314_07697

AVM:	JQX13_06230 JQX13_18330 JQX13_21325 JQX13_29500 JQX13_29505 JQX13_35000

MBD:	MEBOL_000619 MEBOL_004242 MEBOL_006291

CFUS:

CYFUS_001067 CYFUS_005706 CYFUS_006155 CYFUS_006373 CYFUS_007200 CYFUS_007262 CYFUS_007593 CYFUS_007667 CYFUS_009809

CCRO:

CMC5_077490

UAM:	UABAM_00219

SCOR:

J3U87_04665 J3U87_18630 J3U87_25030 J3U87_34815

ZGA:	ZOBELLIA_3906

» show all

Reference

1 [PMID:2276]

Authors

Holmquist B, Vallee BL.

Title

Esterase activity of zinc neutral proteases.

Journal

Biochemistry 15:101-7 (1976)
DOI:10.1021/bi00646a016

Reference

2 [PMID:1012006]

Authors

Wilkes SH, Prescott JM.

Title

Aeromonas neutral protease.

Journal

Methods Enzymol 45:404-15 (1976)
DOI:10.1016/S0076-6879(76)45036-X

Reference

3 [PMID:7000005]

Authors

Bayliss ME, Wilkes SH, Prescott JM.

Title

Aeromonas neutral protease: specificity toward extended substrates.

Journal

Arch Biochem Biophys 204:214-9 (1980)
DOI:10.1016/0003-9861(80)90026-0

Reference

4 [PMID:3123480]

Authors

Wilkes SH, Bayliss ME, Prescott JM.

Title

Critical ionizing groups in Aeromonas neutral protease.

Journal

J Biol Chem 263:1821-5 (1988)

Reference

5 [PMID:1551587]

Authors

David VA, Deutch AH, Sloma A, Pawlyk D, Ally A, Durham DR

Title

Cloning, sequencing and expression of the gene encoding the extracellular neutral protease, vibriolysin, of Vibrio proteolyticus.

Journal

Gene 112:107-12 (1992)
DOI:10.1016/0378-1119(92)90310-L

Sequence

[ag:AAA27548]

Other DBs

ExplorEnz - The Enzyme Database:

3.4.24.25

IUBMB Enzyme Nomenclature:

3.4.24.25

ExPASy - ENZYME nomenclature database:

3.4.24.25

BRENDA, the Enzyme Database:

3.4.24.25

CAS:	69598-88-5

DBGET integrated database retrieval system