KEGG   ENZYME: 3.4.24.25
Entry
EC 3.4.24.25                Enzyme                                 
Name
vibriolysin;
Aeromonas proteolytica neutral proteinase;
aeromonolysin
Class
Hydrolases;
Acting on peptide bonds (peptidases);
Metalloendopeptidases
Reaction(IUBMB)
Preferential cleavage of bonds with bulky hydrophobic groups in P2 and P1'. Phe at P1' is the most favoured residue, which distinguished this enzyme from thermolysin
Comment
Thermostable enzyme from Vibrio proteolyticus (formerly Aeromonas proteolytica). Specificity related to, but distinct from, those of thermolysin and bacillolysin [1]. A zinc metallopeptidase in family M4 (thermolysin family). Formerly included in EC 3.4.24.4
History
EC 3.4.24.25 created 1972 as EC 3.4.24.4, part transferred 1992 to EC 3.4.24.25, modified 1997
Orthology
K08604  vibriolysin
Genes
GKN: PVT67_03015 PVT67_09445
XSA: SB85_15700
XCS: KHA79_18145
XTH: G4Q83_21425
XAG: HEP73_04011(nprV)
XAS: HEP74_03868(hap)
XHA: PG878_03170
XRY: QN244_03010
XSO: RAB70_06355
XIN: Q7W82_00070
XAI: HEP75_03930(nprV)
XTS: AB3X08_20250
XMD: AB3X07_21005
XBN: AB3X10_20995
LAB: LA76x_2473(nprV) LA76x_2475
LAQ: GLA29479_1171(empA) GLA29479_1174(hap)
LCP: LC55x_2887(nprV) LC55x_2889 LC55x_2891
LGU: LG3211_2739 LG3211_2741(nprV) LG3211_4445
LEZ: GLE_2741(nprV) GLE_2743(nprV)
LEM: LEN_2283 LEN_2284
LYT: DWG18_10025
LYA: RDV84_18675 RDV84_18680
LHX: LYSHEL_12180
LCAS: LYSCAS_12180
LLZ: LYB30171_01014
LMB: C9I47_0200
LSF: I8J32_005115
LSX: H8B22_08015
THES: FHQ07_05060
THEH: G7079_11500
TCN: H9L16_08945
TBV: H9L17_11900
THEX: ICG51_000849
FSG: LQ771_02990 LQ771_09765 LQ771_13930
FED: LQ772_03675 LQ772_11020 LQ772_15560
RHD: R2APBS1_0936
RGL: CS053_13835
RTH: LRK53_06130 LRK53_12690
DTX: ATSB10_19710
DYG: DYGSA30_42470
LRZ: BJI69_12675
LPY: FIV34_05775
LANH: KR767_01775
LFL: IM816_04480
LAEG: L2Y94_16255
LAEO: L2Y97_05845
LAES: L2Y96_06360
XBA: C7S18_19035
RBD: ALSL_0921 ALSL_2568
VCH: VC_A0865
VCF: IR04_03775
VCS: MS6_A0903
VCE: Vch1786_II0554(hap)
VCQ: EN18_02630
VCJ: VCD_000466
VCI: O3Y_17553
VCO: VC0395_0371(hap)
VCR: VC395_A0889(hap)
VCM: VCM66_A0824(hap)
VCL: VCLMA_B0638
VCX: VAA049_2123(hap)
VCZ: VAB027_324(hap)
VVU: VV2_0974
VVY: VVA1465
VVM: VVMO6_04367
VVL: VV93_v1c43660(lasB)
VPA: VPA0755
VPB: VPBB_A0695
VPF: M634_21715
VPH: VPUCM_20654
VHA: VIBHAR_02509 VIBHAR_05515
VCA: M892_00890 M892_23945
VEX: VEA_000274
VDB: AL552_06510
VOW: A9237_15790 A9237_19765
VRO: BSZ04_00415
VSP: VS_1267(vsm)
VFU: vfu_B00520
VAN: VAA_01306
LAG: N175_16430
VAU: VANGNB10_cII0919c(empA)
VCY: IX92_04640 IX92_17695 IX92_22445
VCT: JV59_17150 JV59_21375 JV59_34105
VTU: IX91_16525
VEU: IXK98_02965
VSI: MTO69_17135
VFL: AL536_05555
VMI: AL543_06040
VGA: BSQ33_18790
VQI: CCZ37_14020 CCZ37_14710
VCC: FAZ90_06635 FAZ90_18460
VAQ: FIV01_09435(hap2) FIV01_17270(nprV1) FIV01_19305(nprV2)
VSR: Vspart_04278(nprV)
VKA: BTD91_00265 BTD91_17540
VNV: IF132_01670
VGI: MID13_09035 MID13_15870
VSL: LTQ54_15765 LTQ54_16510 LTQ54_21280
VPL: SA104470976_03599(hap)
VTI: CEQ48_01775
VCRA: IS519_00895 IS519_16885
VNP: KW548_13075 KW548_18330 KW548_21875
VLE: ISX51_08050 ISX51_19380 ISX51_22605
VRU: RND59_16645
VIS: VspSTUT16_32930
VIO: AB2S62_15725
PHOT: L4174_010765
PATE: NNL38_21925
GKD: K6Q96_22635 K6Q96_23505
SALY: E8E00_07740 E8E00_14545
SKS: FCN78_05640 FCN78_13840
SCOT: HBA18_05895 HBA18_14490
SPRO: N7E60_06120 N7E60_16375
PAGR: E2H98_08005
SDN: Sden_3015
SAZ: Sama_0428 Sama_1201
SBL: Sbal_3874
SBM: Shew185_0439
SBN: Sbal195_0456
SBP: Sbal223_0465
SBT: Sbal678_0463
SBS: Sbal117_4032
SBB: Sbal175_0534
SHE: Shewmr4_2515
SHM: Shewmr7_2583
SHN: Shewana3_2681
SWD: Swoo_0557 Swoo_3129
SVO: SVI_2120
SHF: CEQ32_07390
SPSW: Sps_01606 Sps_02676 Sps_03413 Sps_03524
SBJ: CF168_07495
SHEW: CKQ84_10610 CKQ84_19125
SKH: STH12_00210(hap_1) STH12_00763(lasB) STH12_04148(hap_2)
SLIT: JQC75_02050 JQC75_05895
SDK: KHX94_06455
SPSH: FM037_17665
SYK: KDN34_02045
SCYP: JYB88_01960 JYB88_06140
SAVI: JYB87_16680
SSEM: JYB85_02135 JYB85_06435
SACH: K0H61_11395
SZH: K0H63_02290 K0H63_06520
SMAY: K0H60_13535
SOG: RA178_02405
SHEM: HWQ47_03255
THAT: H3N35_00170 H3N35_00175
TACT: SG35_000160 SG35_000165 SG35_029565
TVD: SG34_029320 SG34_029325 SG34_032645
PSM: PSM_B0576
PIA: PI2015_3586
PPHE: PP2015_3255 PP2015_3842
PBW: D172_014080 D172_014110 D172_019810
PRR: AT705_01970 AT705_21455 AT705_23865 AT705_23895 AT705_24095
PLZ: S4054249_19905 S4054249_21035 S4054249_21745
PALN: B0W48_01265
PPIS: B1L02_19675 B1L02_23455 B1L02_23510
PEA: PESP_b0778
PTU: PTUN_a1933 PTUN_b0532
PTD: PTET_b0708
PSEN: PNC201_18505(empA) PNC201_22380(hap)
PDJ: D0907_17045
PAGA: PAGA_b0882
PCAR: PC2016_3245
PXI: J5O05_00755 J5O05_21200
PVB: J5X90_00010 J5X90_18620 J5X90_20530 J5X90_22665 J5X90_22945 J5X90_22950
PSAZ: PA25_02030 PA25_34250
PFLI: CTT31_18695 CTT31_20005
PMAZ: R5H13_20175
PXSI: EJ103_16845
PSJS: GZH31_17790
AGZ: M0C34_05585
MVS: MVIS_1898(empA) MVIS_2920(empA)
MYA: MORIYA_1508(nprV) MORIYA_2361(nprV)
MMAA: FR932_02520
MOQ: HWV03_05935 HWV03_07230
TTU: TERTU_3351
SAGA: M5M_18570
SLIO: R50072_11910
MII: BTJ40_13250 BTJ40_13270
MICT: FIU95_09120(nprV)
MHYD: GTQ55_10450
MICZ: GL2_38190
MVB: MJO52_11575
MPAF: R5R33_01940
MSPN: M8T91_11200 M8T91_11435 M8T91_13530
LPN: lpg0019
LPH: LPV_0023
LPO: LPO_0020
LPU: LPE509_03226
LPM: LP6_0020
LPF: lpl0020
LPP: lpp0019
LPC: LPC_0020
LPA: lpa_00026
LPE: lp12_0019
LLO: LLO_0045
LFA: LFA_0023
LSH: CAB17_00130 CAB17_04525
LGT: E4T54_04155
LCJ: NCTC11976_00699(hap)
LWA: SAMEA4504053_0021(hap) SAMEA4504053_0892(nprV)
LSS: NCTC12082_01281(hap)
LANT: TUM19329_03270
TMC: LMI_0117 LMI_1381
PSAL: PSLF89_34
TIG: THII_0643
CYS: NYP54_08535
RHH: E0Z06_02880
WOC: BA177_08085
HCH: HCH_02479
HAHE: ENC22_11265 ENC22_12285
HAHH: O5O45_06305
GSN: YC6258_00785 YC6258_04970
RFO: REIFOR_01115 REIFOR_01121 REIFOR_02033
PLEI: Q9312_13695 Q9312_17865
AHA: AHA_0851
AHY: AHML_04340
AHD: AI20_14990
AHR: V428_04535
AHP: V429_04535
AHJ: V469_18780
AHH: RY45_04760
AHI: VU14_18245
AAJ: BOQ57_04220
AEO: O23A_p4509
AVR: B565_3414
AVO: AMS64_19910
AMED: B224_0573
ASR: WL1483_2962(nprV)
ADH: CK627_08250
ACAV: VI35_03960
AEA: C2U39_04290
AES: C2U30_09950
AEL: NCTC12917_00828(nprV)
AALL: I6G90_19165
AJD: I6H43_07255
ARIV: KYK33_16480
ASAA: KXJ75_04455
AEJ: E5E97_08830
ABES: IU367_17485
AERO: CK910_00720
AERZ: E5E96_11610
KKO: Kkor_0311
KGE: TQ33_2045
KPD: CW740_01305
KAM: SR900_00520
IFL: C1H71_01435
CARI: FNU76_01840 FNU76_12870
CKS: H9L41_00435
RDP: RD2015_2455
ROI: N4261_15900
KIA: G8A07_16855
PAQA: K9V56_005565
MIU: ABE85_14380
JAB: VN23_08790
CPRA: CPter91_2011
UPV: EJN92_01400
UCY: RF679_07215 RF679_15065
HOE: IMCC20628_04027
BBAN: J4G43_043050
DALK: DSCA_47230
MXA: MXAN_6106(fibA)
MSD: MYSTI_02756 MYSTI_03758 MYSTI_06723
MYM: A176_000783
MFB: MFUL124B02_15035 MFUL124B02_35290 MFUL124B02_41125
MYX: QEG98_33555
MLAN: JY572_07835 JY572_12650 JY572_33505
CCX: COCOR_00680(fibA1) COCOR_05260(empA) COCOR_05722(nprV) COCOR_06634(fibA)
MFU: LILAB_15735
MMAS: MYMAC_005892
PPAK: JY651_00165 JY651_05150 JY651_11750 JY651_11805 JY651_15935 JY651_16660 JY651_27790 JY651_43805 JY651_45685 JY651_51415
AGE: AA314_01740 AA314_04229 AA314_05554 AA314_07697
AVM: JQX13_06230 JQX13_18330 JQX13_21325 JQX13_29500 JQX13_29505 JQX13_35000
MBD: MEBOL_000619 MEBOL_004242 MEBOL_006291
CFUS: CYFUS_001067 CYFUS_005706 CYFUS_006155 CYFUS_006373 CYFUS_007200 CYFUS_007262 CYFUS_007593 CYFUS_007667 CYFUS_009809
CCRO: CMC5_077490
UAM: UABAM_00219
UHL: UABHE_002435
LBV: JYG24_03605
SCOR: J3U87_04665 J3U87_18630 J3U87_25030 J3U87_34815
ZGA: ZOBELLIA_3906
 » show all
Reference
1  [PMID:2276]
  Authors
Holmquist B, Vallee BL.
  Title
Esterase activity of zinc neutral proteases.
  Journal
Biochemistry 15:101-7 (1976)
DOI:10.1021/bi00646a016
Reference
2  [PMID:1012006]
  Authors
Wilkes SH, Prescott JM.
  Title
Aeromonas neutral protease.
  Journal
Methods Enzymol 45:404-15 (1976)
DOI:10.1016/S0076-6879(76)45036-X
Reference
3  [PMID:7000005]
  Authors
Bayliss ME, Wilkes SH, Prescott JM.
  Title
Aeromonas neutral protease: specificity toward extended substrates.
  Journal
Arch Biochem Biophys 204:214-9 (1980)
DOI:10.1016/0003-9861(80)90026-0
Reference
4  [PMID:3123480]
  Authors
Wilkes SH, Bayliss ME, Prescott JM.
  Title
Critical ionizing groups in Aeromonas neutral protease.
  Journal
J Biol Chem 263:1821-5 (1988)
Reference
5  [PMID:1551587]
  Authors
David VA, Deutch AH, Sloma A, Pawlyk D, Ally A, Durham DR
  Title
Cloning, sequencing and expression of the gene encoding the extracellular neutral protease, vibriolysin, of Vibrio proteolyticus.
  Journal
Gene 112:107-12 (1992)
DOI:10.1016/0378-1119(92)90310-L
  Sequence
[ag:AAA27548]
Other DBs
ExplorEnz - The Enzyme Database: 3.4.24.25
IUBMB Enzyme Nomenclature: 3.4.24.25
ExPASy - ENZYME nomenclature database: 3.4.24.25
BRENDA, the Enzyme Database: 3.4.24.25
CAS: 69598-88-5

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Gene (654)   
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Protein sequence (51)   
   UniProt (43)   
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DNA sequence (166)   
   RefSeq(nuc) (6)   
   GenBank (83)   
   EMBL (77)   
3D Structure (3)   
   PDB (3)   
Protein domain (9)   
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All databases (883)   

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