KEGG   ENZYME: 3.4.24.28
Entry
EC 3.4.24.28                Enzyme                                 

Name
bacillolysin;
Bacillus metalloendopeptidase;
Bacillus subtilis neutral proteinase;
anilozyme P 10;
Bacillus metalloproteinase;
Bacillus neutral proteinase;
megateriopeptidase
Class
Hydrolases;
Acting on peptide bonds (peptidases);
Metalloendopeptidases
Reaction(IUBMB)
Similar, but not identical, to that of thermolysin
Comment
Variants of this enzyme have been found in species of Bacillus including B. subtilis [1,6], B. amyloliquefaciens [5], B. megaterium (megateriopeptidase, [2]), B. mesentericus [10], B. cereus [3,8,9] and B. stearothermophilus [7]. In peptidase family M4 (thermolysin family). Formerly included in EC 3.4.24.4
History
EC 3.4.24.28 created 1972 as EC 3.4.24.4, part transferred 1992 to EC 3.4.24.28
Orthology
K01400  bacillolysin
Genes
ETR: ETAE_2022
ETD: ETAF_1830
ETC: ETAC_09270
VMI: AL543_03465
SHL: Shal_3483
SVO: SVI_0640
SAGA: M5M_09630
HCH: HCH_00828
HAHE: ENC22_03390
PKT: AT984_18215
CCX: COCOR_00799(npr) COCOR_01296(nprM) COCOR_05687(nprS) COCOR_06712(nprM1)
SCL: sce4498
BSU: BSU14700(nprE)
BSR: I33_1652
BSL: A7A1_0557
BSH: BSU6051_14700(nprE)
BSUT: BSUB_01599(nprE)
BSUL: BSUA_01599(nprE)
BSUS: Q433_08425
BSS: BSUW23_07570(nprE)
BST: GYO_1812
BSO: BSNT_07973(nprE)
BSQ: B657_14700(nprE)
BSX: C663_1514(nprE)
BAQ: BACAU_1430(npr)
BYA: BANAU_1393(npr)
BAMP: B938_07575
BAML: BAM5036_1392(nprE)
BAMA: RBAU_1436(nprE)
BAMN: BASU_1416(nprE)
BAMB: BAPNAU_2298(npr)
BAMT: AJ82_08345
BAMY: V529_14130(nprE)
BMP: NG74_01512(npr)
BAO: BAMF_1546(nprE)
BAZ: BAMTA208_09795(nprE)
BQL: LL3_01578(nprE)
BXH: BAXH7_01996(npr)
BQY: MUS_1567(nprE)
BAMI: KSO_012080
BAMC: U471_14890
BAMF: U722_07735
BAN: BA_0599
BAR: GBAA_0599
BAT: BAS0567
BAI: BAA_0683
BANT: A16_06580
BANR: A16R_06670
BANV: DJ46_1349 DJ46_5058(npr)
BCE: BC0602
BCA: BCE_0667
BCZ: BCE33L0511(npr) pE33L466_0375(npr)
BCQ: BCQ_0665(npr) BCQ_PI139
BAL: BACI_c06090(npr1)
BCER: BCK_05145
BTK: BT9727_0509(npr)
BTL: BALH_0539(nprE) BALH_2915(npr)
BTT: HD73_0673
BTHI: BTK_03405
BTM: MC28_5326(npr)
BTG: BTB_c06140(npr1) BTB_c55530(nprS)
BTI: BTG_18155
BTW: BF38_1848(npr)
BWW: bwei_5002(npr)
BMYO: BG05_5325(npr)
BMYC: DJ92_2128(nprB) DJ92_3204(npr) DJ92_5598(nprM)
BMQ: BMQ_2326(nprM)
BMD: BMD_2285(nprM)
BMEG: BG04_4696(nprM)
BJS: MY9_1610
BACP: SB24_02420
BACB: OY17_10370
BACY: QF06_06375
BACL: BS34A_16300(nprE)
BALM: BsLM_1565
GEA: GARCT_02867(nprT)
ANM: GFC28_3256(npr)
ANL: GFC29_2178(npr)
HHD: HBHAL_4199(npr2) HBHAL_4829(npr4)
VPN: A21D_00581(nprE)
ESI: Exig_2940
EAN: Eab7_2752
BBE: BBR47_13290(npr)
PPM: PPSC2_20195(npr)
PPO: PPM_4046(npr)
PPQ: PPSQR21_040810(lasB)
PPOY: RE92_17000
PLV: ERIC2_c17790(npr1) ERIC2_c23330(npr2) ERIC2_c25850(npr3)
PRI: PRIO_3659(npr)
CAC: CA_C2290(nprE) CA_C2517(nrpE)
CAE: SMB_G2324 SMB_G2552(nprE)
CBO: CBO1446
CBA: CLB_1466(npr-1) CLB_1467(npr-2) CLB_1468(npr-3) CLB_1470(npr-5) CLB_1471(npr-6)
CBH: CLC_1478(npr-1) CLC_1479(npr-2) CLC_1480(npr-3) CLC_1482(npr-5) CLC_1483(npr-6)
CBY: CLM_1605(npr) CLM_1606(npr) CLM_1607(npr) CLM_1608(npr) CLM_1609
CBL: CLK_0923(npr) CLK_0924(npr) CLK_0925(npr) CLK_0926(npr) CLK_0927
CBB: CLD_3104 CLD_3105(npr) CLD_3107(npr) CLD_3108(npr)
CBI: CLJ_B1544(npr_1) CLJ_B1545(npr_2) CLJ_B1547(npr_4) CLJ_B1548(npr_5)
CBF: CLI_1525(npr-1) CLI_1526(npr-2) CLI_1527(npr-3) CLI_1529(npr-5) CLI_1530
CSQ: CSCA_0144
CLD: CLSPO_c14710(nprM3) CLSPO_c14720(nprM4) CLSPO_c14730(nprM2)
HHW: NCTC503_02371(npr)
HSC: HVS_05615(nprM)
MVA: Mvan_3789
MGI: Mflv_2748
MVQ: MYVA_3606
KFL: Kfla_3564
HAU: Haur_0437
ATM: ANT_27970
CHU: CHU_1208
FAE: FAES_3027(npr)
HSW: Hsw_4005
FLM: MY04_3789
GFL: GRFL_1314
FJO: Fjoh_0886
FIN: KQS_02660
MESQ: C7H62_0231
CHZ: CHSO_4198
MAC: MA_2075
METM: MSMTP_2051
 » show all
Reference
1  [PMID:4967801]
  Authors
Morihara K, Tsuzuki H, Oka T.
  Title
Comparison of the specificities of various neutral proteinases from microorganisms.
  Journal
Arch Biochem Biophys 123:572-88 (1968)
DOI:10.1016/0003-9861(68)90179-3
Reference
2  [PMID:4980359]
  Authors
Millet J, Acher R.
  Title
[Specificity of megateriopeptidase: an amino-endopeptidase with hydrophobic characteristics]
  Journal
Eur J Biochem 9:456-62 (1969)
DOI:10.1111/j.1432-1033.1969.tb00631.x
Reference
3  [PMID:5002444]
  Authors
Feder J, Keay L, Garrett LR, Cirulis N, Moseley MH, Wildi BS.
  Title
Bacillus cereus neutral protease.
  Journal
Biochim Biophys Acta 251:74-8 (1971)
DOI:10.1016/0005-2795(71)90061-4
Reference
4  [PMID:2276]
  Authors
Holmquist B, Vallee BL.
  Title
Esterase activity of zinc neutral proteases.
  Journal
Biochemistry 15:101-7 (1976)
DOI:10.1021/bi00646a016
Reference
5  [PMID:6090391]
  Authors
Vasantha N, Thompson LD, Rhodes C, Banner C, Nagle J, Filpula D.
  Title
Genes for alkaline protease and neutral protease from Bacillus amyloliquefaciens contain a large open reading frame between the regions coding for signal sequence and mature protein.
  Journal
J Bacteriol 159:811-9 (1984)
DOI:10.1128/JB.159.3.811-819.1984
  Sequence
[bao:BAMF_1546]
Reference
6  [PMID:6090407]
  Authors
Yang MY, Ferrari E, Henner DJ.
  Title
Cloning of the neutral protease gene of Bacillus subtilis and the use of the cloned gene to create an in vitro-derived deletion mutation.
  Journal
J Bacteriol 160:15-21 (1984)
DOI:10.1128/JB.160.1.15-21.1984
  Sequence
[bsu:BSU14700]
Reference
7  [PMID:2993245]
  Authors
Takagi M, Imanaka T, Aiba S.
  Title
Nucleotide sequence and promoter region for the neutral protease gene from Bacillus stearothermophilus.
  Journal
J Bacteriol 163:824-31 (1985)
DOI:10.1128/JB.163.3.824-831.1985
  Sequence
Reference
8  [PMID:3092843]
  Authors
Sidler W, Niederer E, Suter F, Zuber H.
  Title
The primary structure of Bacillus cereus neutral proteinase and comparison with thermolysin and Bacillus subtilis neutral proteinase.
  Journal
Biol Chem Hoppe Seyler 367:643-57 (1986)
DOI:10.1515/bchm3.1986.367.2.643
  Sequence
Reference
9  [PMID:3127592]
  Authors
Pauptit RA, Karlsson R, Picot D, Jenkins JA, Niklaus-Reimer AS, Jansonius JN.
  Title
Crystal structure of neutral protease from Bacillus cereus refined at 3.0 A resolution and comparison with the homologous but more thermostable enzyme thermolysin.
  Journal
J Mol Biol 199:525-37 (1988)
DOI:10.1016/0022-2836(88)90623-7
  Sequence
Reference
10 [PMID:2302386]
  Authors
Stoeva S, Kleinschmidt T, Mesrob B, Braunitzer G.
  Title
Primary structure of a zinc protease from Bacillus mesentericus strain 76.
  Journal
Biochemistry 29:527-34 (1990)
DOI:10.1021/bi00454a029
Other DBs
ExplorEnz - The Enzyme Database: 3.4.24.28
IUBMB Enzyme Nomenclature: 3.4.24.28
ExPASy - ENZYME nomenclature database: 3.4.24.28
BRENDA, the Enzyme Database: 3.4.24.28
CAS: 9080-56-2

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