KEGG ENZYME: 3.4.24.3

ENZYME: 3.4.24.3

Entry

EC 3.4.24.3                 Enzyme

Name

microbial collagenase;
Clostridium histolyticum collagenase;
clostridiopeptidase A;
collagenase A;
collagenase I;
Achromobacter iophagus collagenase;
collagenase;
aspergillopeptidase C;
nucleolysin;
azocollase;
metallocollagenase;
soycollagestin;
Clostridium histolyticum proteinase A;
clostridiopeptidase II;
MMP-8;
clostridiopeptidase I;
collagen peptidase;
collagen protease;
collagenase MMP-1;
metalloproteinase-1;
kollaza;
matrix metalloproteinase-1;
MMP-1;
matrix metalloproteinase-8;
matirx metalloproteinase-18;
interstitial collagenase

Class

Hydrolases;
Acting on peptide bonds (peptidases);
Metalloendopeptidases

Reaction(IUBMB)

Digestion of native collagen in the triple helical region at !Gly bonds. With synthetic peptides, a preference is shown for Gly at P3 and P1', Pro and Ala at P2 and P2', and hydroxyproline, Ala or Arg at P3'

Comment

Six species of metalloendopeptidase acting on native collagen can be isolated from the medium of Clostridium histolyticum. Class I has forms alpha (68 kDa), beta (115 kDa) and gamma (79 kDa); class II has delta (100 kDa), epsilon (110 kDa) and zeta (125 kDa). The two classes are immunologically crossreactive, but have significantly different sequences, and different specificities such that their actions on collagen are complementary. The enzymes also act as peptidyl-tripeptidases. Variants of the enzyme have been purified from Bacillus cereus [10], Empedobacter collagenolyticum [4], Pseudomonas marinoglutinosa [1], and species of Vibrio, Vibrio B-30 (ATCC 21250) [2] and V. alginolyticus (previously Achromobacter iophagus) [3,8]. Also known from Streptomyces sp. [9]. The Vibrio enzyme is the type example of peptidase family M9.

History

EC 3.4.24.3 created 1961 as EC 3.4.4.19, transferred 1972 to EC 3.4.24.3 (EC 3.4.24.8 created 1978, incorporated 1992, EC 3.4.99.5 created 1972, incorporated 1978)

Orthology

K01387

microbial collagenase

Genes

SYM:	K6K13_07620

SGL:

SG1493

ANS:	ArsFIN_36420

LAB:	LA76x_0536 LA76x_4670

LAQ:	GLA29479_2609 GLA29479_4489

LCP:	LC55x_0373

LGU:	LG3211_0395

LEZ:

GLE_0220

LEM:

LEN_4723

LYA:	RDV84_05505

TBV:	H9L17_07585

XBC:	ELE36_02390

VCH:

VC_1650

VCF:	IR04_13470

VCS:

MS6_1430

VCE:	Vch1786_I1148

VCQ:	EN18_11440

VCJ:	VCD_002727

VCI:

O3Y_08015

VCO:	VC0395_A1256

VCM:	VCM66_1589(vcc)

VCL:	VCLMA_A1438

VCX:	VAA049_1153(prt)

VCZ:	VAB027_2297(prt)

VVU:

VV2_1146

VVY:

VVA1672

VVM:	VVMO6_04535

VVL:	VV93_v1c45320

VPA:	VP1340 VPA0459

VPB:	VPBB_1263 VPBB_A0415

VPK:	M636_01520 M636_15115

VPF:	M634_08605 M634_24235

VPH:	VPUCM_1884 VPUCM_21180

VHA:	VIBHAR_01786

VCA:	M892_04240

VAG:	N646_0375 N646_4702

VEX:	VEA_001372 VEA_003663

VDB:	AL552_01125 AL552_14900

VHR:	AL538_01715 AL538_15610

VOW:	A9237_01060 A9237_02490

VNI:	VIBNI_B0933(prt)

VAN:

VAA_02998

LAG:	N175_18145

VAU:	VANGNB10_cII0222(expA)

VCY:	IX92_23635 IX92_23650

VCT:	JV59_24215 JV59_24230

VTU:	IX91_16760 IX91_16775

VEU:	IXK98_02730 IXK98_02745

VMI:	AL543_17555

VQI:	CCZ37_17920

VNL:	D3H41_06630 D3H41_24590

VCC:	FAZ90_19575

VKA:	BTD91_18335

VSL:	LTQ54_23095

VPL:	SA104470976_01384(prt)

VTI:	CEQ48_10820

VNP:	KW548_19220 KW548_19240

VLE:	ISX51_16665

PGH:	FH974_23545 FH974_23860

SALY:

E8E00_07750

SKS:	FCN78_05630

SCOT:

HBA18_05885

SPRO:

N7E60_06110

PAGR:

E2H98_16820

SON:

SO_0639

SAZ:

Sama_1517

SBL:

Sbal_3732

SBM:	Shew185_0630

SBN:	Sbal195_0657

SBP:	Sbal223_0653

SBT:	Sbal678_0673

SBS:	Sbal117_3887

SBB:	Sbal175_0718

SLO:	Shew_1685 Shew_3732

SSE:

Ssed_0039

SPL:	Spea_0035 Spea_2754

SHL:	Shal_0031 Shal_2840

SWD:	Swoo_0042 Swoo_0648

SWP:	swp_0061 swp_0865 swp_1489 swp_3353 swp_4890

SVO:	SVI_0067 SVI_3541 SVI_3717 SVI_4320

SHF:	CEQ32_12920

SPSW:

Sps_00073 Sps_02927 Sps_04859

SMAV:

CFF01_10920 CFF01_18510

SHEW:

CKQ84_11320

SALG:

BS332_04105 BS332_07110

SKH:	STH12_00069 STH12_03699

SLIT:

JQC75_00185 JQC75_07455

SPSH:

FM037_00205 FM037_21005 FM037_23220 FM037_24125

SEUR:

FM038_000190 FM038_003185 FM038_006590 FM038_011915

SCYP:

JYB88_00155 JYB88_05610

SSEM:

JYB85_00165 JYB85_05820

SXM:	MKD32_17815

SVM:	KDH10_003248

SAEG:

K0H80_08575 K0H80_19115

SDEO:

D0436_19245

SHAO:

K0H81_11420 K0H81_19620

SZH:	K0H63_00180

SSPA:

K0I31_08730 K0I31_19490

SHNS:

K0J45_08555 K0J45_19140

SRHS:

K0I63_08625 K0I63_19350

SOG:	RA178_03100

CPS:

CPS_2237

COM:	CMT41_10020 CMT41_10045 CMT41_14560

LSD:	EMK97_03630 EMK97_04110

THAT:

H3N35_05815 H3N35_11185 H3N35_17285

TACT:

SG35_006005 SG35_011785 SG35_018280

TVD:	SG34_011010 SG34_017635 SG34_023445

PSEO:

OM33_11865 OM33_13060

PPHE:

PP2015_2384 PP2015_3904

PRR:	AT705_02105

PLZ:	S4054249_06990

PALN:

B0W48_13740 B0W48_16285 B0W48_19315

PPIS:

B1L02_09755

PSPO:

PSPO_a1890(colA) PSPO_a2170(colA)

PTU:	PTUN_a1257(colA) PTUN_b0328

PSEN:

PNC201_06960

PDJ:	D0907_20835

PXI:	J5O05_00370

PSAZ:

PA25_12310 PA25_24690

PFLI:

CTT31_07070

PMAZ:

R5H13_07615

FBL:	Fbal_0027 Fbal_1101

MVS:

MVIS_3532

MMAA:

FR932_00900

MOQ:	HWV03_04165

SAGA:

M5M_14702

HCH:

HCH_05788

HAHE:

ENC22_04995 ENC22_20490

HAHH:

O5O45_16110 O5O45_31220

BSAN:

CHH28_12995 CHH28_18270

AHA:

AHA_0517

AHY:	AHML_02655

AHD:	AI20_16805

AHR:	V428_02800

AHP:	V429_02800

AHJ:	V469_20520

AHH:	RY45_03030

AHI:	VU14_19905

AAJ:	BOQ57_02500

ASA:

ASA_3723

AEO:	O23A_p4180

AVR:

B565_0474

AVO:	AMS64_03860

AMED:

B224_4851

ASR:	WL1483_1472

ADH:	CK627_06380

ACAV:

VI35_18890

AEM:	CK911_14200

AEA:	C2U39_01730

AES:	C2U30_11765

AEL:	NCTC12917_00428 NCTC12917_01287

AALL:

I6G90_13330

AJD:	I6H43_13325

ARIV:

KYK33_01660

AEJ:	E5E97_06860

ABES:

IU367_02235

CVI:	CV_2001 CV_3255

CVC:	BKX93_00240 BKX93_07020

CHRO:

CXB49_05205 CXB49_09935

CHRI:

DK842_01145 DK842_17090

CHRB:

DK843_00215 DK843_06425

CRZ:	D1345_08110 D1345_20290

CHRM:

FYK34_09295 FYK34_10450

CHAE:

CH06BL_17500 CH06BL_41830

IOD:	EJO50_00910

IFL:	C1H71_12535

CARI:

FNU76_03015 FNU76_03230 FNU76_20410

CKS:	H9L41_10360

BMA:

BMAA0694

BML:	BMA10229_0773

BMN:	BMA10247_A1736

BMAL:

DM55_4743

BMAE:

DM78_3247

BMAQ:

DM76_3697

BMAI:

DM57_12100

BMAF:

DM51_4400

BMAZ:

BM44_4733

BPS:	BPSS0666 BPSS0827

BPM:	BURPS1710b_A2230 BURPS1710b_A2419

BPL:	BURPS1106A_A0900 BURPS1106A_A1142(colA)

BPD:	BURPS668_A0988 BURPS668_A1216(colA)

BPSE:

BDL_3897 BDL_4114

BPSM:

BBQ_5310 BBQ_5528

BPSU:

BBN_4066 BBN_4286

BPSD:

BBX_5734 BBX_5954

BPZ:	BP1026B_II0739 BP1026B_II0910

BPQ:	BPC006_II0939 BPC006_II1159

BPK:	BBK_3613 BBK_3823

BPSH:

DR55_4509 DR55_4684

BPSA:

BBU_5204 BBU_5376

BPSO:

X996_3849 X996_4062

BUT:	X994_3736 X994_3950

BTE:	BTH_II1578

BTQ:

BTQ_4865

BTJ:

BTJ_3490

BTZ:

BTL_4339

BTD:

BTI_5644

BTV:

BTHA_3618

BTHE:

BTN_3990

BTHM:

BTRA_4103

BTHA:

DR62_4026

BTHL:

BG87_4321

BOK:

DM82_4997

BOC:

BG90_5598

BUU:	WS70_21515

BSAV:

WS86_22840

BHG:	I6G56_23845

BUD:	AQ610_26545

BUR:	Bcep18194_C6876

BCON:

NL30_31580

BUB:

BW23_582

BSTG:

WT74_18870

BARI:

NLX30_34200

BUL:

BW21_5331

JAB:	VN23_11850

CARE:

LT85_1390

MNR:	ACZ75_14080

MASS:

CR152_02130

MASY:

DPH57_14170

MANC:

IV454_08230

MASZ:

C9I28_07890

TCT:	PX653_15185

RUG:	QC826_24220

UPV:	EJN92_18320

UPI:	EJG51_001605

UND:	UNDKW_5675

PAQA:

K9V56_019515

MXA:

MXAN_6205

MSD:	MYSTI_00797

MFB:	MFUL124B02_04445

BAN:	BA_0555 BA_3299 BA_3584

BAR:	GBAA_0555 GBAA_3299 GBAA_3584

BAT:	BAS0523 BAS3060 BAS3323

BAH:	BAMEG_1049 BAMEG_1322 BAMEG_4031

BAI:	BAA_0637 BAA_3338 BAA_3612

BAX:	H9401_0526 H9401_3141 H9401_3407

BANT:

A16_06120 A16_33180 A16_35910

BANR:

A16R_06210 A16R_33590 A16R_36360

BANS:

BAPAT_0532 BAPAT_3158 BAPAT_3431

BANH:

HYU01_03025 HYU01_16200 HYU01_17535

BANV:

DJ46_2039 DJ46_2304 DJ46_5013

BCE:	BC0556 BC2466 BC3161 BC3412 BC3529 BC3762

BCA:	BCE_0616 BCE_3539

BCZ:	BCE33L0466(colA) BCE33L3238(colA) pE33L466_0145(colA)

BCR:	BCAH187_A0684 BCAH187_A3542

BCB:	BCB4264_A0591 BCB4264_A3027 BCB4264_A3415 BCB4264_A3636

BCU:	BCAH820_0611 BCAH820_3538

BCG:	BCG9842_B1680 BCG9842_B1830 BCG9842_B4748

BCQ:	BCQ_0620(colA) BCQ_3316(colA)

BCX:	BCA_0591 BCA_3602

BAL:	BACI_c05630(colC) BACI_c34720(colA2)

BNC:	BCN_0532 BCN_3327

BCF:	bcf_02860 bcf_17385

BCER:

BCK_05370 BCK_17635

BCEF:

BcrFT9_00524 BcrFT9_02687

BCY:	Bcer98_0486

BTK:	BT9727_0466(colA) BT9727_3286(colA)

BTL:	BALH_0495(colA) BALH_3172

BTB:	BMB171_C0475 BMB171_C2222 BMB171_C2705 BMB171_C3206

BTT:	HD73_0625 HD73_2839 HD73_2953 HD73_3659 HD73_3800

BTHR:

YBT1520_03075 YBT1520_13940 YBT1520_14525 YBT1520_18015 YBT1520_18750

BTHI:

BTK_03160 BTK_14070 BTK_14655 BTK_18005 BTK_18690

BTC:	CT43_CH0476 CT43_CH3007 CT43_CH3345 CT43_CH3469

BTF:	YBT020_03025 YBT020_17280

BTM:	MC28_2207 MC28_2558 MC28_2678 MC28_5277

BTG:	BTB_c05690(colA1) BTB_c31330(colA2) BTB_c34780 BTB_c36010(colA3)

BTI:	BTG_01795 BTG_02525 BTG_18395

BTN:	BTF1_00485 BTF1_14350 BTF1_15395

BTHT:

H175_328p153 H175_ch0479 H175_ch3057 H175_ch3398 H175_ch3526

BTHU:

YBT1518_02940 YBT1518_13825 YBT1518_16590 YBT1518_18525 YBT1518_32215

BTW:	BF38_1804 BF38_4683

BTHY:

AQ980_12320 AQ980_13010 AQ980_16355 AQ980_16915 AQ980_27850

BWE:	BcerKBAB4_0468 BcerKBAB4_2821 BcerKBAB4_3229 BcerKBAB4_5655

BWW:	bwei_1412(colA2) bwei_2005(colA5) bwei_5050(colA4) bwei_5337(colA1)

BMYO:

BG05_2484 BG05_5371 BG05_5681

BTY:	Btoyo_0303 Btoyo_0743 Btoyo_3232

BMYC:

DJ92_3542 DJ92_727

BBY:	CY96_01925 CY96_13895 CY96_14600 CY96_16045 CY96_16640

BWD:	CT694_03230 CT694_16160 CT694_18765 CT694_30070

BTRO:

FJR70_10510 FJR70_25000

BMOB:

MLA2C4_02975 MLA2C4_18295

BNT:	GSN03_03070 GSN03_26925

BPAC:

LMD38_09505(colA) LMD38_23280(colA)

BPAN:

NLJ82_03005(colA) NLJ82_16620(colA)

BALU:

QRY64_05155(colA) QRY64_18660(colA) QRY64_20260(colA)

BARD:

QRY57_07435 QRY57_15280(colA)

LSP:

Bsph_2533

LFU:	HR49_13620

LIU:	OU989_13015

BBE:	BBR47_37890(colA) BBR47_42950(colA)

BLR:	BRLA_c021960

BFM:	BP422_17155

BRW:	GOP56_03780

PLV:	ERIC2_c40370(colA)

PTHI:

NDS46_19880

PDY:	QJQ58_02175

CPE:

CPE0173

CPF:	CPF_0166(colA)

CPR:	CPR_0162(colA)

CTC:	CTC_p33(colT)

CTET:

BN906_02951(colT)

CNO:	NT01CX_1295

CBO:	CBO1620(colA)

CBA:	CLB_1638(colG)

CBH:

CLC_1647

CBY:	CLM_1861(colG)

CBL:	CLK_1084(colG)

CBK:	CLL_A1484(colG)

CBB:	CLD_2936(colG)

CBI:	CLJ_B1730(colG)

CBN:

CbC4_0444

CBT:	CLH_1400(colG)

CBF:

CLI_1698

CBJ:	H04402_01689

CLD:	CLSPO_c16750(colA)

CTAE:

BGI42_06275

CCHV:

BTM20_05850

CARG:

RSJ17_19405 RSJ17_19410

CSEP:

CP523_01745

CCOH:

SAMEA4530647_1638(colA)

CGAS:

J1C67_18165

HHW:	NCTC503_01706(colA_1) NCTC503_01968(colA_2)

CPRF:

K7H06_19995

PBIF:

KXZ80_14905

PSOR:

RSJ16_05935

VGU:	HYG85_06865

SCO:	SCO5912(SC10A5.17)

SGR:

SGR_1615

SGB:

WQO_27370

SFI:

SFUL_5845

SANL:

KZO11_29015

SVL:	Strvi_4027

SRC:	M271_02750

SSOI:

I1A49_46520

SVE:

SVEN_7132

SCI:	B446_03865

SLV:	SLIV_09050

SGU:	SGLAU_01440

SVT:	SVTN_02160

STRE:

GZL_00476

SLD:

T261_0204

SLC:	SL103_15115 SL103_22460

STRM:

M444_26285

STRC:

AA958_14100

SPRI:

SPRI_1204

SCZ:	ABE83_06910

SRW:	TUE45_00795

STRT:

A8713_27885

SGS:	AVL59_26705

SALW:

CP975_30670

SPLU:

LK06_030995

SSIA:

A7J05_05805

SVU:	B1H20_27950

SPUN:

BFF78_39785

SGV:	B1H19_33805

SLAU:

SLA_7008

SALF:

SMD44_06979(colA)

SALJ:

SMD11_0557(colA)

SLX:	SLAV_10155(colA) SLAV_36175

STRO:

STRMOE7_04630 STRMOE7_32945

SFK:	KY5_6960 KY5_7845

SNZ:	DC008_03070

SRJ:

SRO_0318

SLK:	SLUN_03935 SLUN_30420

SCYA:

EJ357_42920

STIR:

DDW44_24170

SKA:	CP970_00210 CP970_05295

SGZ:	C0216_08460

SVN:	CP980_07885

SRK:	FGW37_02070

SFIC:

EIZ62_06905

SGF:	HEP81_00314

SCAV:

CVT27_26900

SBY:	H7H31_26315

SRIM:

CP984_36195

SSUB:

CP968_01275 CP968_08505

SCIN:

CP977_26205

SFUG:

CNQ36_29380

SCHA:

CP983_39980

SGX:	H4W23_02520 H4W23_02860

SSPB:

CP982_05145

SNF:	JYK04_06361(colA)

SPLA:

CP981_03300 CP981_33800 CP981_33810

SBRO:

GQF42_02900

SATA:

C5746_33145 C5746_33285

SHUN:

DWB77_01513

SCYG:

S1361_02665

SFEU:

IM697_26860

SDW:	K7C20_02220 K7C20_03150 K7C20_36820

SCAL:

I6J39_27815

SAUH:

SU9_001675 SU9_002370

SFB:	CP974_00945

SMOB:

J7W19_00915

SXN:	IAG42_35195

SHK:	J2N69_32180

SANU:

K7396_32270

STRY:

EQG64_28075

SDD:	D9753_33580

SACT:

DMT42_11395

SINE:

KI385_05630 KI385_37985 KI385_37995 KI385_41745

SAOV:

G3H79_37230 G3H79_38075

SDUR:

M4V62_40675

SYAN:

NRK68_26425

STAA:

LDH80_04525 LDH80_36665

SNIG:

HEK616_27970 HEK616_75150

SXT:	KPP03845_105647(colA)

STUD:

STRTU_000660 STRTU_006628

SENG:

OJ254_23600

SGK:	PET44_24840

SYUN:

MOV08_01810

SARG:

HKX69_32685

SOV:	QZH56_05275

SCT:	SCAT_p1663

SCY:	SCATT_p00570

KSK:	KSE_20890 KSE_25070 KSE_60110 KSE_67060

KAB:	B7C62_29390

KIT:	CFP65_0291 CFP65_0889 CFP65_1275 CFP65_6049

KIS:	HUT16_08970 HUT16_28520

STRH:

GXP74_18675

LARI:

KI794_13715

ARR:	ARUE_232p01330

GMY:

XH9_10895

SRO:

Sros_3516

SEN:

SACE_3082

SESP:

BN6_23720

KUT:	JJ691_21020

LED:	BBK82_31925

ALO:

CRK56022

MIL:

ML5_4364

MTUA:

CSH63_05105 CSH63_05520

MCHL:

PVK74_30710

DROS:

Drose_24115 Drose_37190

CAI:	Caci_0616 Caci_7152

SNA:

Snas_2903

LIL:

LA_0872

LIE:

LIF_A0713

LIC:

LIC_12760

LIS:

LIL_12850

LBJ:	LBJ_0742(colA)

LBL:	LBL_2336(colA)

LMAY:

DPV73_01695

LWL:	FHG68_15900 FHG68_17515

LNO:	MAL08_13845

LKR:	FH602_08940

SCOR:

J3U87_18340 J3U87_27310 J3U87_32380

COY:	HF329_14750

FLP:	LK994_10410

PEX:	IZT61_17660

FOR:	FORMB_09080

» show all

Reference

Authors

Hanada, K., Mizutani, T., Yamagishi, M., Tsuji, H., Misaki, T. Sawada, J.

Title

The isolation of collagenase and its enzymological and physico-chemical properties.

Journal

Agric Biol Chem 37:1771-1781 (1973)

Reference

2 [PMID:210785]

Authors

Merkel JR, Dreisbach JH.

Title

Purification and characterization of a marine bacterial collagenase.

Journal

Biochemistry 17:2857-63 (1978)
DOI:10.1021/bi00607a025

Reference

3 [PMID:6250633]

Authors

Heindl MC, Fermandjian S, Keil B.

Title

Circular dichroism comparative studies of two bacterial collagenases and thermolysin.

Journal

Biochim Biophys Acta 624:51-9 (1980)
DOI:10.1016/0005-2795(80)90224-X

Reference

4 [PMID:6530724]

Authors

Waid DD, Warren RJ, Pence DB.

Title

Elaeophora schneideri Wehr and Dickmans, 1935 in white-tailed deer from the Edwards Plateau of Texas.

Journal

J Wildl Dis 20:342-5 (1984)
DOI:10.7589/0090-3558-20.4.342

Reference

5 [PMID:6087888]

Authors

Bond MD, Van Wart HE.

Title

Characterization of the individual collagenases from Clostridium histolyticum.

Journal

Biochemistry 23:3085-91 (1984)
DOI:10.1021/bi00308a036

Reference

6 [PMID:6087889]

Authors

Bond MD, Van Wart HE.

Title

Relationship between the individual collagenases of Clostridium histolyticum: evidence for evolution by gene duplication.

Journal

Biochemistry 23:3092-9 (1984)
DOI:10.1021/bi00308a037

Reference

7 [PMID:3002445]

Authors

Van Wart HE, Steinbrink DR.

Title

Complementary substrate specificities of class I and class II collagenases from Clostridium histolyticum.

Journal

Biochemistry 24:6520-6 (1985)
DOI:10.1021/bi00344a032

Reference

Authors

Tong, N.T., Tsugita, A. and Keil-Dlouha, V.

Title

Purification and characterization of two high-molecular-mass forms of Achromobacter collagenase.

Journal

Biochim Biophys Acta 874:296-304 (1986)

Reference

9 [PMID:2822678]

Authors

Endo A, Murakawa S, Shimizu H, Shiraishi Y.

Title

Purification and properties of collagenase from a Streptomyces species.

Journal

J Biochem (Tokyo) 102:163-70 (1987)
DOI:10.1093/oxfordjournals.jbchem.a122028

Reference

10 [PMID:3040751]

Authors

Makinen KK, Makinen PL.

Title

Purification and properties of an extracellular collagenolytic protease produced by the human oral bacterium Bacillus cereus (strain Soc 67).

Journal

J Biol Chem 262:12488-95 (1987)

Other DBs

ExplorEnz - The Enzyme Database:

3.4.24.3

IUBMB Enzyme Nomenclature:

3.4.24.3

ExPASy - ENZYME nomenclature database:

3.4.24.3

BRENDA, the Enzyme Database:

3.4.24.3

CAS:

9001-12-1

DBGET integrated database retrieval system