Entry
Name
microbial collagenase;
Clostridium histolyticum collagenase;
clostridiopeptidase A;
collagenase A;
collagenase I;
Achromobacter iophagus collagenase;
collagenase;
aspergillopeptidase C;
nucleolysin;
azocollase;
metallocollagenase;
soycollagestin;
Clostridium histolyticum proteinase A;
clostridiopeptidase II;
MMP-8;
clostridiopeptidase I;
collagen peptidase;
collagen protease;
collagenase MMP-1;
metalloproteinase-1;
kollaza;
matrix metalloproteinase-1;
MMP-1;
matrix metalloproteinase-8;
matirx metalloproteinase-18;
interstitial collagenase
Class
Hydrolases;
Acting on peptide bonds (peptidases);
Metalloendopeptidases
BRITE hierarchy
Reaction(IUBMB)
Digestion of native collagen in the triple helical region at !Gly bonds. With synthetic peptides, a preference is shown for Gly at P3 and P1', Pro and Ala at P2 and P2', and hydroxyproline, Ala or Arg at P3'
Comment
Six species of metalloendopeptidase acting on native collagen can be isolated from the medium of Clostridium histolyticum. Class I has forms alpha (68 kDa), beta (115 kDa) and gamma (79 kDa); class II has delta (100 kDa), epsilon (110 kDa) and zeta (125 kDa). The two classes are immunologically crossreactive, but have significantly different sequences, and different specificities such that their actions on collagen are complementary. The enzymes also act as peptidyl-tripeptidases. Variants of the enzyme have been purified from Bacillus cereus [10], Empedobacter collagenolyticum [4], Pseudomonas marinoglutinosa [1], and species of Vibrio, Vibrio B-30 (ATCC 21250) [2] and V. alginolyticus (previously Achromobacter iophagus) [3,8]. Also known from Streptomyces sp. [9]. The Vibrio enzyme is the type example of peptidase family M9.
History
EC 3.4.24.3 created 1961 as EC 3.4.4.19, transferred 1972 to EC 3.4.24.3 (EC 3.4.24.8 created 1978, incorporated 1992, EC 3.4.99.5 created 1972, incorporated 1978)
Orthology
Genes
LAB : LA76x_0536 LA76x_4670
LAQ : GLA29479_2609 GLA29479_4489
VPB : VPBB_1263 VPBB_A0415
VPK : M636_01520 M636_15115
VPF : M634_08605 M634_24235
VPH : VPUCM_1884 VPUCM_21180
VEX : VEA_001372 VEA_003663
VDB : AL552_01125 AL552_14900
VHR : AL538_01715 AL538_15610
VOW : A9237_01060 A9237_02490
VAU : VANGNB10_cII0222(expA)
VCY : IX92_23635 IX92_23650
VCT : JV59_24215 JV59_24230
VTU : IX91_16760 IX91_16775
VEU : IXK98_02730 IXK98_02745
VNL : D3H41_06630 D3H41_24590
VPL : SA104470976_01384(prt)
VNP : KW548_19220 KW548_19240
PGH : FH974_23545 FH974_23860
SWP : swp_0061 swp_0865 swp_1489 swp_3353 swp_4890
SVO : SVI_0067 SVI_3541 SVI_3717 SVI_4320
SPSW : Sps_00073 Sps_02927 Sps_04859
SMAV : CFF01_10920 CFF01_18510
SALG : BS332_04105 BS332_07110
SKH : STH12_00069 STH12_03699
SLIT : JQC75_00185 JQC75_07455
SPSH : FM037_00205 FM037_21005 FM037_23220 FM037_24125
SEUR : FM038_000190 FM038_003185 FM038_006590 FM038_011915
SCYP : JYB88_00155 JYB88_05610
SSEM : JYB85_00165 JYB85_05820
SAEG : K0H80_08575 K0H80_19115
SHAO : K0H81_11420 K0H81_19620
SSPA : K0I31_08730 K0I31_19490
SHNS : K0J45_08555 K0J45_19140
SRHS : K0I63_08625 K0I63_19350
COM : CMT41_10020 CMT41_10045 CMT41_14560
LSD : EMK97_03630 EMK97_04110
THAT : H3N35_05815 H3N35_11185 H3N35_17285
TACT : SG35_006005 SG35_011785 SG35_018280
TVD : SG34_011010 SG34_017635 SG34_023445
PSEO : OM33_11865 OM33_13060
PPHE : PP2015_2384 PP2015_3904
PALN : B0W48_13740 B0W48_16285 B0W48_19315
PSPO : PSPO_a1890(colA) PSPO_a2170(colA)
PTU : PTUN_a1257(colA) PTUN_b0328
PSAZ : PA25_12310 PA25_24690
HAHE : ENC22_04995 ENC22_20490
HAHH : O5O45_16110 O5O45_31220
BSAN : CHH28_12995 CHH28_18270
AEL : NCTC12917_00428 NCTC12917_01287
CVC : BKX93_00240 BKX93_07020
CHRO : CXB49_05205 CXB49_09935
CHRI : DK842_01145 DK842_17090
CHRB : DK843_00215 DK843_06425
CRZ : D1345_08110 D1345_20290
CHRM : FYK34_09295 FYK34_10450
CHAE : CH06BL_17500 CH06BL_41830
CARI : FNU76_03015 FNU76_03230 FNU76_20410
BPM : BURPS1710b_A2230 BURPS1710b_A2419
BPL : BURPS1106A_A0900 BURPS1106A_A1142(colA)
BPD : BURPS668_A0988 BURPS668_A1216(colA)
BPZ : BP1026B_II0739 BP1026B_II0910
BPQ : BPC006_II0939 BPC006_II1159
BPSH : DR55_4509 DR55_4684
BPSO : X996_3849 X996_4062
BAN : BA_0555 BA_3299 BA_3584
BAR : GBAA_0555 GBAA_3299 GBAA_3584
BAT : BAS0523 BAS3060 BAS3323
BAH : BAMEG_1049 BAMEG_1322 BAMEG_4031
BAI : BAA_0637 BAA_3338 BAA_3612
BAX : H9401_0526 H9401_3141 H9401_3407
BANT : A16_06120 A16_33180 A16_35910
BANR : A16R_06210 A16R_33590 A16R_36360
BANS : BAPAT_0532 BAPAT_3158 BAPAT_3431
BANH : HYU01_03025 HYU01_16200 HYU01_17535
BANV : DJ46_2039 DJ46_2304 DJ46_5013
BCE : BC0556 BC2466 BC3161 BC3412 BC3529 BC3762
BCZ : BCE33L0466(colA) BCE33L3238(colA) pE33L466_0145(colA)
BCR : BCAH187_A0684 BCAH187_A3542
BCB : BCB4264_A0591 BCB4264_A3027 BCB4264_A3415 BCB4264_A3636
BCU : BCAH820_0611 BCAH820_3538
BCG : BCG9842_B1680 BCG9842_B1830 BCG9842_B4748
BCQ : BCQ_0620(colA) BCQ_3316(colA)
BAL : BACI_c05630(colC) BACI_c34720(colA2)
BCER : BCK_05370 BCK_17635
BCEF : BcrFT9_00524 BcrFT9_02687
BTK : BT9727_0466(colA) BT9727_3286(colA)
BTL : BALH_0495(colA) BALH_3172
BTB : BMB171_C0475 BMB171_C2222 BMB171_C2705 BMB171_C3206
BTT : HD73_0625 HD73_2839 HD73_2953 HD73_3659 HD73_3800
BTHR : YBT1520_03075 YBT1520_13940 YBT1520_14525 YBT1520_18015 YBT1520_18750
BTHI : BTK_03160 BTK_14070 BTK_14655 BTK_18005 BTK_18690
BTC : CT43_CH0476 CT43_CH3007 CT43_CH3345 CT43_CH3469
BTF : YBT020_03025 YBT020_17280
BTM : MC28_2207 MC28_2558 MC28_2678 MC28_5277
BTG : BTB_c05690(colA1) BTB_c31330(colA2) BTB_c34780 BTB_c36010(colA3)
BTI : BTG_01795 BTG_02525 BTG_18395
BTN : BTF1_00485 BTF1_14350 BTF1_15395
BTHT : H175_328p153 H175_ch0479 H175_ch3057 H175_ch3398 H175_ch3526
BTHU : YBT1518_02940 YBT1518_13825 YBT1518_16590 YBT1518_18525 YBT1518_32215
BTHY : AQ980_12320 AQ980_13010 AQ980_16355 AQ980_16915 AQ980_27850
BWE : BcerKBAB4_0468 BcerKBAB4_2821 BcerKBAB4_3229 BcerKBAB4_5655
BWW : bwei_1412(colA2) bwei_2005(colA5) bwei_5050(colA4) bwei_5337(colA1)
BMYO : BG05_2484 BG05_5371 BG05_5681
BTY : Btoyo_0303 Btoyo_0743 Btoyo_3232
BBY : CY96_01925 CY96_13895 CY96_14600 CY96_16045 CY96_16640
BWD : CT694_03230 CT694_16160 CT694_18765 CT694_30070
BTRO : FJR70_10510 FJR70_25000
BMOB : MLA2C4_02975 MLA2C4_18295
BNT : GSN03_03070 GSN03_26925
BPAC : LMD38_09505(colA) LMD38_23280(colA)
BPAN : NLJ82_03005(colA) NLJ82_16620(colA)
BALU : QRY64_05155(colA) QRY64_18660(colA) QRY64_20260(colA)
BARD : QRY57_07435 QRY57_15280(colA)
BBE : BBR47_37890(colA) BBR47_42950(colA)
CARG : RSJ17_19405 RSJ17_19410
CCOH : SAMEA4530647_1638(colA)
HHW : NCTC503_01706(colA_1) NCTC503_01968(colA_2)
SLC : SL103_15115 SL103_22460
SLX : SLAV_10155(colA) SLAV_36175
STRO : STRMOE7_04630 STRMOE7_32945
SLK : SLUN_03935 SLUN_30420
SKA : CP970_00210 CP970_05295
SSUB : CP968_01275 CP968_08505
SGX : H4W23_02520 H4W23_02860
SPLA : CP981_03300 CP981_33800 CP981_33810
SATA : C5746_33145 C5746_33285
SDW : K7C20_02220 K7C20_03150 K7C20_36820
SAUH : SU9_001675 SU9_002370
SINE : KI385_05630 KI385_37985 KI385_37995 KI385_41745
SAOV : G3H79_37230 G3H79_38075
STAA : LDH80_04525 LDH80_36665
SNIG : HEK616_27970 HEK616_75150
SXT : KPP03845_105647(colA)
STUD : STRTU_000660 STRTU_006628
KSK : KSE_20890 KSE_25070 KSE_60110 KSE_67060
KIT : CFP65_0291 CFP65_0889 CFP65_1275 CFP65_6049
KIS : HUT16_08970 HUT16_28520
MTUA : CSH63_05105 CSH63_05520
DROS : Drose_24115 Drose_37190
LWL : FHG68_15900 FHG68_17515
SCOR : J3U87_18340 J3U87_27310 J3U87_32380
» show all
Taxonomy
Reference
1
Authors
Hanada, K., Mizutani, T., Yamagishi, M., Tsuji, H., Misaki, T. Sawada, J.
Title
The isolation of collagenase and its enzymological and physico-chemical properties.
Journal
Agric Biol Chem 37:1771-1781 (1973)
Reference
Authors
Merkel JR, Dreisbach JH.
Title
Purification and characterization of a marine bacterial collagenase.
Journal
Reference
Authors
Heindl MC, Fermandjian S, Keil B.
Title
Circular dichroism comparative studies of two bacterial collagenases and thermolysin.
Journal
Reference
Authors
Waid DD, Warren RJ, Pence DB.
Title
Elaeophora schneideri Wehr and Dickmans, 1935 in white-tailed deer from the Edwards Plateau of Texas.
Journal
Reference
Authors
Bond MD, Van Wart HE.
Title
Characterization of the individual collagenases from Clostridium histolyticum.
Journal
Reference
Authors
Bond MD, Van Wart HE.
Title
Relationship between the individual collagenases of Clostridium histolyticum: evidence for evolution by gene duplication.
Journal
Reference
Authors
Van Wart HE, Steinbrink DR.
Title
Complementary substrate specificities of class I and class II collagenases from Clostridium histolyticum.
Journal
Reference
8
Authors
Tong, N.T., Tsugita, A. and Keil-Dlouha, V.
Title
Purification and characterization of two high-molecular-mass forms of Achromobacter collagenase.
Journal
Biochim Biophys Acta 874:296-304 (1986)
Reference
Authors
Endo A, Murakawa S, Shimizu H, Shiraishi Y.
Title
Purification and properties of collagenase from a Streptomyces species.
Journal
Reference
Authors
Makinen KK, Makinen PL.
Title
Purification and properties of an extracellular collagenolytic protease produced by the human oral bacterium Bacillus cereus (strain Soc 67).
Journal
J Biol Chem 262:12488-95 (1987)
Other DBs
ExplorEnz - The Enzyme Database: 3.4.24.3
ExPASy - ENZYME nomenclature database: 3.4.24.3