KEGG   ENZYME: 3.4.24.31
Entry
EC 3.4.24.31                Enzyme                                 
Name
mycolysin;
pronase component;
Streptomyces griseus neutral proteinase;
actinase E;
SGNPI
Class
Hydrolases;
Acting on peptide bonds (peptidases);
Metalloendopeptidases
Reaction(IUBMB)
Preferential cleavage of bonds with hydrophobic residues in P1'
Comment
The enzyme has been characterized from the bacteria Streptomyces griseus, Streptomyces naraensis, and Streptomyces cacaoi. Specificity is similar to that of thermolysin, but the enzyme is much more sensitive to inhibition by sulfanylacetyl-Phe-Leu. Little structural similarity to other bacterial metalloendopeptidases. Type example of peptidase family M5.
History
EC 3.4.24.31 created 1972 as EC 3.4.24.4, part transferred 1992 to EC 3.4.24.31
Orthology
K20744  mycolysin
Genes
STREGZL_08972
SLDT261_7813
SRIMCP984_18635
SMOBJ7W19_16560
SINEKI385_42280
AIHAiant_77060
Reference
1  [PMID:4967801]
  Authors
Morihara K, Tsuzuki H, Oka T.
  Title
Comparison of the specificities of various neutral proteinases from microorganisms.
  Journal
Arch Biochem Biophys 123:572-88 (1968)
DOI:10.1016/0003-9861(68)90179-3
Reference
2  [PMID:5073323]
  Authors
Hiramatsu A, Ouchi T.
  Title
A neutral proteinase from Streptomyces naraensis. 3. An improved purification and some physiochemical properties.
  Journal
J Biochem (Tokyo) 71:767-81 (1972)
DOI:10.1093/oxfordjournals.jbchem.a129826
Reference
3  [PMID:6413206]
  Authors
Blumberg S, Tauber Z.
  Title
Inhibition of metalloendopeptidases by 2-mercaptoacetyl-dipeptides.
  Journal
Eur J Biochem 136:151-4 (1983)
DOI:10.1111/j.1432-1033.1983.tb07719.x
Reference
4  [PMID:2341042]
  Authors
Chang PC, Kuo TC, Tsugita A, Lee YH
  Title
Extracellular metalloprotease gene of Streptomyces cacaoi: structure, nucleotide sequence and characterization of the cloned gene product.
  Journal
Gene 88:87-95 (1990)
DOI:10.1016/0378-1119(90)90063-W
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 3.4.24.31
IUBMB Enzyme Nomenclature: 3.4.24.31
ExPASy - ENZYME nomenclature database: 3.4.24.31
BRENDA, the Enzyme Database: 3.4.24.31
CAS: 153190-34-2

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