KEGG   ENZYME: 3.4.24.33
Entry
EC 3.4.24.33                Enzyme                                 

Name
peptidyl-Asp metalloendopeptidase;
endoproteinase Asp-N;
peptidyl-Asp metalloproteinase
Class
Hydrolases;
Acting on peptide bonds (peptidases);
Metalloendopeptidases
Reaction(IUBMB)
Cleavage of Xaa!Asp, Xaa!Glu and Xaa!cysteic acid bonds
Comment
A metalloenzyme isolated from Pseudomonas fragi. Useful in protein sequencing applications because of its limited specificity. In peptidase family M72.
History
EC 3.4.24.33 created 1992
Orthology
K23743  peptidyl-Asp metalloendopeptidase
Genes
PAO: Pat9b_1661
XBV: XBW1_0130
XCC: XCC0955
XCB: XC_3280
XCA: xcc-b100_3396
XCP: XCR_1151
XCV: XCV1064 XCV3406
XAX: XACM_1015
XAC: XAC1034
XCI: XCAW_03548
XPH: XppCFBP6546_22875(XppCFBP6546P_22875)
SML: Smlt0970
SMT: Smal_0815
SMZ: SMD_0844
PPF: Pput_0320
PPI: YSA_05605
PPUN: PP4_03260
SWD: Swoo_3429
PSM: PSM_B0114
PSPO: PSPO_a1218
PART: PARC_b0067
PAGA: PAGA_b0173
AMAC: MASE_15605
CJA: CJA_0276(cbp6B) CJA_1294
SDE: Sde_3927(cbm6F)
MMAI: sS8_5288
AHA: AHA_0616
DGG: DGI_1524
SVE: SVEN_7265
SFI: SFUL_513
SFK: KY5_4622
DFE: Dfer_2081
 » show all
Reference
1  [PMID:236771]
  Authors
Porzio MA, Pearson AM.
  Title
Isolation of an extracellular neutral proteinase from Pseudomonas fragi.
  Journal
Biochim Biophys Acta 384:235-41 (1975)
DOI:10.1016/0005-2744(75)90112-6
Reference
2  [PMID:7188696]
  Authors
Drapeau GR.
  Title
Substrate specificity of a proteolytic enzyme isolated from a mutant of Pseudomonas fragi.
  Journal
J Biol Chem 255:839-40 (1980)
Reference
3  [PMID:2669754]
  Authors
Ingrosso D, Fowler AV, Bleibaum J, Clarke S.
  Title
Specificity of endoproteinase Asp-N (Pseudomonas fragi): cleavage at glutamyl residues in two proteins.
  Journal
Biochem Biophys Res Commun 162:1528-34 (1989)
DOI:10.1016/0006-291X(89)90848-6
Other DBs
ExplorEnz - The Enzyme Database: 3.4.24.33
IUBMB Enzyme Nomenclature: 3.4.24.33
ExPASy - ENZYME nomenclature database: 3.4.24.33
BRENDA, the Enzyme Database: 3.4.24.33
CAS: 55576-49-3

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