KEGG   ENZYME: 3.4.24.33Help
Entry
EC 3.4.24.33                Enzyme                                 

Name
peptidyl-Asp metalloendopeptidase;
endoproteinase Asp-N;
peptidyl-Asp metalloproteinase
Class
Hydrolases;
Acting on peptide bonds (peptidases);
Metalloendopeptidases
BRITE hierarchy
Reaction(IUBMB)
Cleavage of Xaa!Asp, Xaa!Glu and Xaa!cysteic acid bonds
Comment
A metalloenzyme isolated from Pseudomonas fragi. Useful in protein sequencing applications because of its limited specificity. In peptidase family M72.
History
EC 3.4.24.33 created 1992
Reference
1  [PMID:236771]
  Authors
Porzio MA, Pearson AM.
  Title
Isolation of an extracellular neutral proteinase from Pseudomonas fragi.
  Journal
Biochim Biophys Acta 384:235-41 (1975)
DOI:10.1016/0005-2744(75)90112-6
Reference
2  [PMID:7188696]
  Authors
Drapeau GR.
  Title
Substrate specificity of a proteolytic enzyme isolated from a mutant of Pseudomonas fragi.
  Journal
J Biol Chem 255:839-40 (1980)
Reference
3  [PMID:2669754]
  Authors
Ingrosso D, Fowler AV, Bleibaum J, Clarke S.
  Title
Specificity of endoproteinase Asp-N (Pseudomonas fragi): cleavage at glutamyl residues in two proteins.
  Journal
Biochem Biophys Res Commun 162:1528-34 (1989)
DOI:10.1016/0006-291X(89)90848-6
Other DBs
ExplorEnz - The Enzyme Database: 3.4.24.33
IUBMB Enzyme Nomenclature: 3.4.24.33
ExPASy - ENZYME nomenclature database: 3.4.24.33
BRENDA, the Enzyme Database: 3.4.24.33
CAS: 55576-49-3

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