KEGG   ENZYME: 3.4.24.33
Entry
EC 3.4.24.33                Enzyme                                 
Name
peptidyl-Asp metalloendopeptidase;
endoproteinase Asp-N;
peptidyl-Asp metalloproteinase
Class
Hydrolases;
Acting on peptide bonds (peptidases);
Metalloendopeptidases
Reaction(IUBMB)
Cleavage of Xaa!Asp, Xaa!Glu and Xaa!cysteic acid bonds
Comment
A metalloenzyme isolated from Pseudomonas fragi. Useful in protein sequencing applications because of its limited specificity. In peptidase family M72.
History
EC 3.4.24.33 created 1992
Orthology
K23743  peptidyl-Asp metalloendopeptidase
Genes
RBADH2866_19375
PAOPat9b_1661
XBVXBW1_0130
XCCXCC0955
XCBXC_3280
XCAxcc-b100_3396
XCPXCR_1151
XCVXCV1064 XCV3406
XAXXACM_1015
XACXAC1034
XCIXCAW_03548
XCTJ151_01160
XCJJ158_01157
XCUJ159_01157
XCNJ169_01156
XCWJ162_01156
XCRJ163_01156
XCMJ164_01156
XCFJ172_01151
XFUXFF4834R_pla00230
XAOXAC29_05235
XSASB85_07835 SB85_08055
XTNFD63_05115
XFRBER92_13795 BER92_15345
XVEBJD12_01905 BJD12_18125
XPEBJD13_11595 BJD13_23105
XHRXJ27_00860 XJ27_10265
XGABI317_05960 BI317_16060
XPHXppCFBP6546_22875(XppCFBP6546P_22875)
XVAC7V42_05585 C7V42_14540
XANAC801_22495
XARXB05_21995
XCZEBN15_04075
XEUXSP_001016
XHDLMG31886_33120
XPRMUG10_04890
XAGHEP73_03312
XDYNYR95_05860
XASHEP74_03126
SMLSmlt0970
SMTSmal_0815
BUJBurJV3_0821
SMZSMD_0844
SRHBAY15_0985
STENCCR98_04395
STEMCLM74_04360
STESMG068_04075
SINCDAIF1_08730
SPAQSTNY_R08790
SGENRKE57_03950
PMEXH4W19_16485
LABLA76x_0516 LA76x_1092 LA76x_1409 LA76x_3981(lieA)
LAQGLA29479_2060 GLA29479_3573(lieA) GLA29479_4589
LCPLC55x_0421 LC55x_1143 LC55x_4009(lieA) LC55x_4359
LGULG3211_1059 LG3211_2566 LG3211_3821(lieA)
LEZGLE_0242 GLE_1028 GLE_1441 GLE_3845
LEMLEN_1306 LEN_3884 LEN_4700
LMBC9I47_2626
LSXH8B22_09870
LAUXLA521A_09120
LYARDV84_01510 RDV84_05400 RDV84_09105 RDV84_13720 RDV84_24925
LUGFPZ22_04995
TCNH9L16_07690
DKOI596_1765 I596_604 I596_650
XBAC7S18_01130
PPFPput_0320
PPGPputGB1_2257 PputGB1_4897
PPWPputW619_4908
PPBPPUBIRD1_0329
PPIYSA_05605
PPUNPP4_03260
PMOSO165_017420
PSECCCOS191_0333
PMAMKSS90_01620
KAKKalk_08295
PSPIPS2015_1896
MBAHHYN46_06595 HYN46_06600 HYN46_14700
SWDSwoo_3429
SBKSHEWBE_3871
COVEKO29_13625
THATH3N35_11850
TACTSG35_012465 SG35_019475
TVDSG34_016995
PSMPSM_B0114
PIAPI2015_3157
PPHEPP2015_3913
PBWD172_016615 D172_020850
PRRAT705_23820
PLZS4054249_12625
PALNB0W48_02315 B0W48_03170 B0W48_07280
PPISB1L02_21050
PEAPESP_b0216
PSPOPSPO_a1218
PARTPARC_b0067
PTDPTET_b0146
PSENPNC201_19910
PAGAPAGA_b0173
PCARPC2016_3768
PVBJ5X90_22990
PDVFFU37_17645
PFLICTT31_21730
PMAZR5H13_21780
AMACMASE_15605
AMGAMEC673_15900
AMKAMBLS11_15350
PAEWKIH87_15410
CJACJA_0276(cbp6B) CJA_1294
CEBB0D95_08930 B0D95_08940
CELLCBR65_21775 CBR65_21900
CEGD0C16_00635 D0C16_09470
SDESde_3927(cbm6F)
MIIBTJ40_08990 BTJ40_11780
MICTFIU95_12240 FIU95_12255
MHYDGTQ55_02330
MAHMEALZ_0963
MBUREQU24_15580
MMAIsS8_5288
MSZEMSZNOR_2114 MSZNOR_2155
PLEIQ9312_16675
AHAAHA_0616
AHYAHML_03155
AHDAI20_16255
AHRV428_03310
AHPV429_03310
AHJV469_20010
AHHRY45_03520
AHIVU14_19400
AAJBOQ57_02995
ASRWL1483_1999
ADHCK627_06890
AEJE5E97_07360
CARIFNU76_03255
ETBN7L95_02805 N7L95_21425
MEEDA075_06885
CMETK6K41_08865 K6K41_08870
PLEOOHA_1_03599
SANDH3309_16830
DGGDGI_1524
BDQCIK05_14290
SFISFUL_513
SVESVEN_7265
SCIB446_00585 B446_34705
SVUB1H20_01525
SALFSMD44_08083
SFKKY5_4622
SFICEIZ62_30920
SSPBCP982_36325
SFPQUY26_02875
KABB7C62_01350
NSNEXE58_09945
NOQLN652_11260
PBFCFX0092_A2517
KSTKSMBR1_3925
UAMUABAM_03214
ABACLuPra_04139 LuPra_04140
NKONiako_0622
DFEDfer_2081
DSNHWI92_18180
IRORT717_01015
CAGGHYG79_15140
CIHATE47_12820
CHRYCEY12_11165
CPIPCJF12_17550
CHRSEAG08_07920
CTAINCTC12078_02720
CORZMTP08_01210
CJGNCTC13459_02463
RMRRmar_0099 Rmar_2053
RMGRhom172_0097
NMVNITMOv2_4112
NKFNkreftii_003441
NLIG3M70_00595
 » show all
Reference
1  [PMID:236771]
  Authors
Porzio MA, Pearson AM.
  Title
Isolation of an extracellular neutral proteinase from Pseudomonas fragi.
  Journal
Biochim Biophys Acta 384:235-41 (1975)
DOI:10.1016/0005-2744(75)90112-6
Reference
2  [PMID:7188696]
  Authors
Drapeau GR.
  Title
Substrate specificity of a proteolytic enzyme isolated from a mutant of Pseudomonas fragi.
  Journal
J Biol Chem 255:839-40 (1980)
Reference
3  [PMID:2669754]
  Authors
Ingrosso D, Fowler AV, Bleibaum J, Clarke S
  Title
Specificity of endoproteinase Asp-N (Pseudomonas fragi): cleavage at glutamyl residues in two proteins.
  Journal
Biochem Biophys Res Commun 162:1528-34 (1989)
DOI:10.1016/0006-291x(89)90848-6
Other DBs
ExplorEnz - The Enzyme Database: 3.4.24.33
IUBMB Enzyme Nomenclature: 3.4.24.33
ExPASy - ENZYME nomenclature database: 3.4.24.33
BRENDA, the Enzyme Database: 3.4.24.33
CAS: 55576-49-3

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