Entry
Name
leishmanolysin;
promastigote surface endopeptidase;
glycoprotein gp63;
Leishmania metalloproteinase;
surface acid proteinase;
promastigote surface protease
Class
Hydrolases;
Acting on peptide bonds (peptidases);
Metalloendopeptidases
BRITE hierarchy
Reaction(IUBMB)
Preference for hydrophobic residues at P1 and P1' and basic residues at P2' and P3'. A model nonapeptide is cleaved at -Ala-Tyr!Leu-Lys-Lys-
Comment
A membrane-bound glycoprotein found on the promastigote of various species of Leishmania protozoans. Contains consensus sequence for a zinc-binding site; Z-Tyr-Leu-NHOH is a strong inhibitor. The enzyme can activate its proenzyme by cleavage of the Val100!Val bond. An acid pH optimum is found with certain protein substrates. Type example of peptidase family M8
History
EC 3.4.24.36 created 1992
Orthology
Genes
CSAT : 104725219 104760706 104771388
MINC : 123202619 123229790
GRA : 105769136 105770076 105772974
GHI : 107904944 107905395 107942010 107942859 107943007 107957561
GAB : 108453648 108483520 108484368
HSYR : 120167576 120168232 120168233 120183378 120212853
DZI : 111312674 111314262 111315379
GMX : 100775874 100788818 100807772 100808350
GSJ : 114384842 114393424 114408661 114414675
CCAJ : 109788373 109805793
APRC : 113845803 113866404
TPRA : 123886150 123893599
PSAT : 127082794 127092034
LANG : 109337314 109358336
QSA : O6P43_016138 O6P43_018848
MSYL : 126603788 126632151
CMAX : 111491790 111496088
CMOS : 111454285 111462558
CPEP : 111798888 111805104
MESC : 110606833 110615517
PALZ : 118028084 118034139
CILL : 122296438 122299251
SIND : 105159208 105166296
SSPL : 121755279 121759224 121805584 121809592
SHIS : 125196126 125223504
HAN : 110879497 110926717 110936242
ECAD : 122589553 122608484
CCAV : 112510108 112511776
CSIN : 114315980 114318039
MING : 122073598 122078654
PSOM : 113289294 113296223
TAES : 123086986 123090988 123096003
MFLO : 136455879 136519050
PVIR : 120649868 120687925
MUS : 103978339 135598452 135619936
ZOF : 121989839 121998797 122012133 122017576 122056013
EHI : EHI_042870(111.t00003) EHI_200230(30.t00006)
EIV : EIN_154240 EIN_174510
TET : TTHERM_00289430 TTHERM_00289510 TTHERM_00289520 TTHERM_00289530 TTHERM_00289540 TTHERM_00289570 TTHERM_00289590 TTHERM_00289600 TTHERM_00290610
PTM : GSPATT00012897001 GSPATT00028904001
TBR : Tb10.70.5290 Tb11.02.5310 Tb11.02.5610 Tb11.02.5630 Tb11.02.5640 Tb11.12.0006 Tb927.8.1610 Tb927.8.1620 Tb927.8.1630 Tb927.8.1640
TBG : TbgDal_VIII1230 TbgDal_X2960 TbgDal_XI8480 TbgDal_XI8830 TbgDal_XI8840 TbgDal_XI9750
TCR : 410797.10 505615.10 505989.70 506145.80 506257.50 506321.240 506401.380 506435.370 506867.40 507917.10 507919.10 507993.350 508241.50 508545.40 508609.10 508611.30 508693.100 508699.100 508999.170 509011.80 509205.100 510263.30 510565.150 510657.200 510747.40 510761.80 510873.20 510899.10 511035.10 511151.54 511203.20 511257.60 511277.610 511281.50 511723.10
LMA : LMJF_10_0460(GP63-1) LMJF_10_0465(GP63-2) LMJF_10_0470(GP63-3) LMJF_10_0480(GP63-4) LMJF_28_0570 LMJF_31_2000
LIF : LINJ_10_0490(GP63-1) LINJ_10_0500(GP63-2) LINJ_10_0510(GP63-3) LINJ_10_0520(GP63-3) LINJ_10_0530(GP63-4) LINJ_28_0600 LINJ_28_0610 LINJ_31_2040
LDO : LDBPK_280600 LDBPK_312040
LMI : LMXM_10_0390 LMXM_10_0405 LMXM_10_0460 LMXM_10_0465 LMXM_10_0470 LMXM_28_0570 LMXM_30_2000
LMAT : 92511625(LSCM1_01505) 92513135(LSCM1_03071) 92516946(LSCM1_07044)
LBZ : LBRM_10_0470(GP63-1) LBRM_10_0480(GP63-2) LBRM_10_0500 LBRM_10_0510(GP63-3) LBRM_10_0520 LBRM_10_0530 LBRM_10_0540 LBRM_10_0550(GP63-2) LBRM_10_0560(GP63-4) LBRM_10_0570(GP63-4) LBRM_10_0580(GP63-4) LBRM_10_0590 LBRM_10_0600 LBRM_10_0610 LBRM_31_2260
LPAN : LPMP_100410(GP63-1) LPMP_100420(GP63-2) LPMP_100430(GP63-3) LPMP_100440(GP63-4) LPMP_311850
LOI : 92359251(LSCM4_03305) 92359726(LSCM4_03796)
LEIS : 94177132(GH5_01545) 94179376(GH5_03920)
LENR : 94170022(CUR178_02762)
LEIN : 94186513(JIQ42_02813) 94186702(JIQ42_03013)
PHET : 94289515(JKF63_03435) 94290004(JKF63_03931)
TVA : TVAG_2v0198910 TVAG_2v0438160
» show all
Taxonomy
Reference
Authors
Button LL, McMaster WR
Title
Molecular cloning of the major surface antigen of leishmania.
Journal
Sequence
Reference
Authors
Bouvier J, Bordier C, Vogel H, Reichelt R, Etges R.
Title
Characterization of the promastigote surface protease of Leishmania as a membrane-bound zinc endopeptidase.
Journal
Reference
Authors
Chaudhuri G, Chaudhuri M, Pan A, Chang KP.
Title
Surface acid proteinase (gp63) of Leishmania mexicana. A metalloenzyme capable of protecting liposome-encapsulated proteins from phagolysosomal degradation by macrophages.
Journal
J Biol Chem 264:7483-9 (1989)
Reference
Authors
Bouvier J, Schneider P, Etges R, Bordier C.
Title
Peptide substrate specificity of the membrane-bound metalloprotease of Leishmania.
Journal
Other DBs
ExPASy - ENZYME nomenclature database: 3.4.24.36