KEGG   ENZYME: 3.4.24.40
Entry
EC 3.4.24.40                Enzyme                                 

Name
serralysin;
Pseudomonas aeruginosa alkaline proteinase;
Escherichia freundii proteinase;
Serratia marcescens extracellular proteinase;
Serratia marcescens metalloproteinase;
Pseudomonas aeruginosa alk. protease;
Serratia marcescens metalloprotease
Class
Hydrolases;
Acting on peptide bonds (peptidases);
Metalloendopeptidases
Reaction(IUBMB)
Preferential cleavage of bonds with hydrophobic residues in P1'
Comment
A 50 kDa extracellular endopeptidase from Pseudomonas aeruginosa [1,2,6], Escherichia freundii [3], Serratia marcescens [4,5,6] and Erwinia chrysanthemi [7]. There is broad specificity in cleavage of the insulin B chain, with some species variations. The pH optimum for digesting various proteins is about 9 - 10. In peptidase family M10 (interstitial collagenase family). Formerly included in EC 3.4.24.4
History
EC 3.4.24.40 created 1972 as EC 3.4.24.4, part transferred 1992 to EC 3.4.24.40
Orthology
K01406  serralysin
Genes
HDE: HDEF_0400
LPV: HYN51_12625
YPE: YPO3973
YPK: y3857
YPH: YPC_4478
YPA: YPA_3800
YPN: YPN_3621
YPM: YP_3335
YPP: YPDSF_3337
YPG: YpAngola_A4088
YPZ: YPZ3_2192
YPD: YPD4_3494
YPX: YPD8_3497
YPW: CH59_1921(prtA)
YPJ: CH55_2652(prtA)
YPV: BZ15_3732(prtA)
YPL: CH46_1084(prtA)
YPS: YPTB3814
YPO: BZ17_2771(prtA)
YPY: YPK_0118
YPB: YPTS_4028
YPQ: DJ40_2581(prtA)
YPU: BZ21_3166(prtA)
YPR: BZ20_2278(prtA)
YPC: BZ23_3440(prtA)
YPF: BZ19_3302(prtA)
YEN: YE4052
YEY: Y11_31431
YEW: CH47_3679(prtA)
YET: CH48_1504(papra)
YAL: AT01_2628(prtA) AT01_2629(aprA)
YFR: AW19_3402(prtA)
YIN: CH53_1609(prtA) CH53_3001(prtA) CH53_3002(prtA)
YKR: CH54_2388
YRO: CH64_2458(prtG)
YRU: BD65_101(prtA)
SPLY: Q5A_000940 Q5A_018425(prtA)
ECA: ECA2785(prtW)
PATR: EV46_13620
PATO: GZ59_17740(prtW)
PCT: PC1_1553
PEC: W5S_2894
DDD: Dda3937_03860(prtC) Dda3937_03861(prtA)
EAM: EAMY_3581(prtA)
EAY: EAM_3368(prtA)
MINT: C7M51_01041(prtC)
MTHI: C7M52_01776(prtC)
PLU: plu0655(prtA)
PAY: PAU_00605
XBO: XBJ1_0491
XBV: XBW1_4194(prtA)
XNE: XNC1_4025
XNM: XNC2_3878(prtA)
XDO: XDD1_3329(prtA)
XPO: XPG1_2747(prtA)
MMK: MU9_1121
ANS: ArsFIN_00200 ArsFIN_02820(prtB_1) ArsFIN_08610(prtC_2) ArsFIN_08620(prtA_1) ArsFIN_18490(prtC_3) ArsFIN_38040(prtA_3)
HPAZ: K756_10290
HPAS: JL26_08850
HPAK: JT17_07530
MVE: X875_1730
BTRA: F544_22240
RPNE: NCTC8284_00863(exsH)
LCP: LC55x_1074(aprA)
PAE: PA1249(aprA)
PAEV: N297_1288(aprA)
PAEI: N296_1288(aprA)
PAU: PA14_48060(aprA)
PAP: PSPA7_4143(aprA)
PAG: PLES_40631(aprA)
PAF: PAM18_3782(aprA)
PNC: NCGM2_2131(aprA)
PAEB: NCGM1900_1327(aprA)
PAEP: PA1S_19670
PAEM: U769_19510
PAEL: T223_20760
PAEU: BN889_01328(aprA)
PAEG: AI22_14295
PAEC: M802_1285(aprA)
PAEO: M801_1288(aprA)
PPT: PPS_1275
PPUT: L483_20685
PPUD: DW66_1560
PSB: Psyr_3163
PVD: CFBP1590__3261(aprA)
PFL: PFL_2483(aprX) PFL_3210(aprA)
PPRC: PFLCHA0_c25470(aprA1) PFLCHA0_c32400(aprA2)
PPRO: PPC_2525 PPC_3236(aprA)
PMAN: OU5_0345
PEN: PSEEN1550(aprA)
PSES: PSCI_4933
PSEC: CCOS191_3824(aprA)
PSIL: PMA3_13735
ACB: A1S_1073
ABAD: ABD1_24060
ABAZ: P795_4860
ACC: BDGL_001271(cyaA)
CPS: CPS_1809
ASIP: AQUSIP_20240(prtC)
METL: U737_23800
MEJ: Q7A_2414
TIG: THII_1552
TKM: TK90_1310
HCO: LOKO_01629(algE1)
DNO: DNO_0235
HYF: DTO96_100940(prtB) DTO96_101604(cya_8)
LIM: L103DPR2_01173(prtA) L103DPR2_02207(prtC_1) L103DPR2_02830(prtC_2) L103DPR2_02851(algE7)
EBA: ebA7184
CURE: CUREO_1543
SULJ: SJPD1_0935
NIS: NIS_1398
DDE: Dde_0594
DMA: DMR_34230
DAS: Daes_2897
DPI: BN4_20041
PPRF: DPRO_1373
MXA: MXAN_5392
HOH: Hoch_3034
MHUA: MCHK_0376
MES: Meso_0616
SME: SMa0034
SMD: Smed_5287
ARA: Arad_4711
ATF: Ach5_07640(rzcA)
RLE: RL0790
LAA: WSI_01255
LSO: CKC_02265
BJA: bll6027
BRA: BRADO6535
BBT: BBta_4186
BRS: S23_22440
PHL: KKY_2563
PLEO: OHA_1_01202(cya_2) OHA_1_01203(cya_3)
HDI: HDIA_4818
CCR: CC_0746
CCS: CCNA_00783(sapA)
SIL: SPO0362
RUT: FIU92_19305(prtC)
RSP: RSP_0230
RDE: RD1_3460
PDE: Pden_0822
KVL: KVU_2014
KVU: EIO_2494
OTM: OSB_33270(prtB)
MALG: MALG_05062
RHC: RGUI_1058
SPSE: SULPSESMR1_01732(hlyA)
ROH: FIU89_09075(hlyA3) FIU89_21930(algE2)
LVS: LOKVESSMR4R_03807(algE7)
MARU: FIU81_04290(cya2) FIU81_15520(prtC)
ROT: FIV09_07230(prtC) FIV09_07335(hlyA2)
SSAN: NX02_17550
SINB: SIDU_05485
ADO: A6F68_02706(prtC)
ALB: AEB_P0089
TMO: TMO_1315
MAI: MICA_92
MAN: A11S_86
PMW: B2K_17365
MANA: MAMMFC1_02196(hlyA)
SHY: SHJG_5268
SRW: TUE45_03911(cya)
SFK: KY5_3469
ALO: CRK57363
AFS: AFR_03680
SYG: sync_1260
SYNR: KR49_13515
HHG: XM38_036590(frpC)
PMT: PMT_1878
PRM: EW15_0487
AMR: AM1_4208
CYT: cce_0913
AVA: Ava_3160
NAZ: Aazo_4916
DOU: BMF77_02243(algE5) BMF77_03295(hlyA_2)
MFF: MFFC18_33530(prtC_3) MFFC18_36160(prtB) MFFC18_51100(apxIA_2)
TTF: THTE_4224
GES: VT84_05180(apxIA)
EAO: BD94_2699
MPW: MPR_2201
CLI: Clim_2271
NEV: NTE_01436
 » show all
Reference
1  [PMID:4967801]
  Authors
Morihara K, Tsuzuki H, Oka T.
  Title
Comparison of the specificities of various neutral proteinases from microorganisms.
  Journal
Arch Biochem Biophys 123:572-88 (1968)
DOI:10.1016/0003-9861(68)90179-3
Reference
2  [PMID:4199986]
  Authors
Morihara K, Tsuzuki H, Oka T.
  Title
On the specificity of Pseudomonas aeruginosa alkaline proteinase with synthetic peptides.
  Journal
Biochim Biophys Acta 309:414-29 (1973)
DOI:10.1016/0005-2744(73)90040-5
  Sequence
[pae:PA1249]
Reference
3  [PMID:4212288]
  Authors
Nakajima M, Mizusawa K, Yoshida F.
  Title
Purification and properties of an extracellular proteinase of psychrophilic Escherichia freundii.
  Journal
Eur J Biochem 44:87-96 (1974)
DOI:10.1111/j.1432-1033.1974.tb03460.x
Reference
4  [PMID:383155]
  Authors
Decedue CJ, Broussard EA 2nd, Larson AD, Braymer HD.
  Title
Purification and characterization of the extracellular proteinase of Serratia marcescens.
  Journal
Biochim Biophys Acta 569:293-301 (1979)
DOI:10.1016/0005-2744(79)90065-2
Reference
5  [PMID:6380155]
  Authors
Doerr M, Traub WH.
  Title
Purification and characterization of two Serratia marcescens proteases.
  Journal
Zentralbl Bakteriol Mikrobiol Hyg [A] 257:6-19 (1984)
Reference
6  [PMID:3016665]
  Authors
Nakahama K, Yoshimura K, Marumoto R, Kikuchi M, Lee IS, Hase T, Matsubara H.
  Title
Cloning and sequencing of Serratia protease gene.
  Journal
Nucleic Acids Res 14:5843-55 (1986)
DOI:10.1093/nar/14.14.5843
  Sequence
Reference
7  [PMID:2211513]
  Authors
Dahler GS, Barras F, Keen NT.
  Title
Cloning of genes encoding extracellular metalloproteases from Erwinia chrysanthemi EC16.
  Journal
J Bacteriol 172:5803-15 (1990)
DOI:10.1128/JB.172.10.5803-5815.1990
  Sequence
Reference
8  [PMID:2123832]
  Authors
Okuda K, Morihara K, Atsumi Y, Takeuchi H, Kawamoto S, Kawasaki H, Suzuki K, Fukushima J.
  Title
Complete nucleotide sequence of the structural gene for alkaline proteinase from Pseudomonas aeruginosa IFO 3455.
  Journal
Infect Immun 58:4083-8 (1990)
  Sequence
[pae:PA1249]
Other DBs
ExplorEnz - The Enzyme Database: 3.4.24.40
IUBMB Enzyme Nomenclature: 3.4.24.40
ExPASy - ENZYME nomenclature database: 3.4.24.40
BRENDA, the Enzyme Database: 3.4.24.40
CAS: 70851-98-8

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