KEGG   ENZYME: 3.4.24.55
Entry
EC 3.4.24.55                Enzyme                                 
Name
pitrilysin;
Escherichia coli protease III;
protease Pi;
proteinase Pi;
PTR;
Escherichia coli metalloproteinase Pi
Class
Hydrolases;
Acting on peptide bonds (peptidases);
Metalloendopeptidases
Reaction(IUBMB)
Preferential cleavage of -Tyr16! Leu- and -Phe25! Tyr-bonds of oxidized insulin B chain. Also acts on other substrates of less than 7 kDa such as insulin and glucagon
Comment
From the periplasmic space of Escherichia coli. Inhibited by EDTA and 1,10-phenanthroline; not thiol-dependent. Type example of peptidase family M16
History
EC 3.4.24.55 created 1992 as EC 3.4.99.44, transferred 1993 to EC 3.4.24.55 (EC 3.4.99.45 created 1992, incorporated 1993)
Orthology
K01407  protease III
Genes
ECOb2821(ptrA)
ECJJW2789(ptr)
ECDECDH10B_2991(ptrA)
EBWBWG_2556(ptrA)
ECOKECMDS42_2327(ptr)
ECEZ4138(ptr)
ECSECs_3678(ptrA)
ECFECH74115_4086(ptrA)
ETWECSP_3773(ptr)
ELXCDCO157_3433
EOIECO111_3549(ptr)
EOJECO26_3893(ptr)
EOHECO103_3380(ptr)
ECOOECRM13514_3684(ptr)
ECOHECRM13516_3548(ptr)
ESLO3K_05405
ESOO3O_20245
ESMO3M_05450
ECKEC55989_3097(ptr)
ECGE2348C_3090(ptr)
EOKG2583_3475(ptrA)
ELRECO55CA74_16555
ELHETEC_3008
ECWEcE24377A_3141(ptrA)
EUNUMNK88_3505
ECPECP_2833
ENAECNA114_2879(ptr)
ECOSEC958_5045(ptrA)
ECVAPECO1_3684(ptrA)
ECOAAPECO78_17710
ECXEcHS_A2967(ptrA)
ECMEcSMS35_2968(ptrA)
ECYECSE_3078
ECRECIAI1_2929(ptr)
ECQECED1_3277(ptr)
EUMECUMN_3148(ptr)
ECTECIAI39_3240(ptr)
EOCCE10_3248(ptrA)
EBRECB_02669(ptr)
EBLECD_02669(ptrA)
EBEB21_02630(ptrA)
EBDECBD_0904
ECIUTI89_C3222(ptrA)
EIHECOK1_3225(ptrA)
ECZECS88_3116(ptr)
ECCc3415(ptr)
ELOEC042_3017(ptrA)
ELNNRG857_13935
ESEECSF_2636
ECLEcolC_0894
EKOEKO11_0920
EKFKO11_08700(ptrA)
EABECABU_c31170(ptr)
EDHEcDH1_0870
EDJECDH1ME8569_2728(ptrA)
ELUUM146_02320
ELWECW_m3063(ptrA)
ELLWFL_14960(ptrA)
ELCi14_3137(ptr)
ELDi02_3137(ptr)
ELPP12B_c2915(ptrA)
ELFLF82_1765(ptrA)
ECOLLY180_14485
ECOIECOPMV1_03108(ptrA)
ECOJP423_15605
EFEEFER_2754(ptr)
EALEAKF1_ch3195(ptrA)
EMAC1192_21470
ESZFEM44_04025
STYSTY3133(ptr)
STTt2903(ptr)
SEXSTBHUCCB_30600
SENTTY21A_14675
STMSTM2995(ptr)
SEOSTM14_3610(ptr)
SEVSTMMW_29561
SEYSL1344_2973(ptr)
SEMSTMDT12_C30450
SEJSTMUK_2983(ptr)
SEBSTM474_3141(ptr)
SEFUMN798_3254(ptr)
SETUSTU288_15140
SETCCFSAN001921_02095
SENRSTMDT2_28941(ptr)
SENDDT104_29911(ptr)
SENICY43_15620
SEENSE451236_21070
SPTSPA2860(ptr)
SEKSSPA2663
SPQSPAB_03726
SEISPC_3052(ptr)
SECSCH_2933(ptr)
SEHSeHA_C3207
SHBSU5_03483
SENHCFSAN002069_17245
SEEHSEEH1578_01025
SEESNSL254_A3221
SENNSN31241_41050
SEWSeSA_A3158
SEASeAg_B3141
SENSQ786_14480
SEDSeD_A3321
SEGSG2903(ptr)
SELSPUL_3003(ptr)
SEGASPUCDC_2989(ptr)
SETSEN2837(ptr)
SENAAU38_14415
SENOAU37_14425
SENVAU39_14420
SENQAU40_16230
SENLIY59_15020
SENJCFSAN001992_18600
SEECCFSAN002050_21330
SEEBSEEB0189_005150
SENBBN855_30550
SENEIA1_14415
SENCSEET0819_21840
SESSARI_04665
SBGSBG_2601(ptr)
SBZA464_3011
SBVN643_12735
SALZEOS98_04455
SFLSF2832(ptr)
SFXS3029(ptr)
SFVSFV_2899(ptr)
SFESFxv_3105(ptrA)
SFNSFy_4036
SFSSFyv_4114
SFTNCTC1_03117(ptrA)
SSNSSON_2978(ptr)
SBOSBO_2711(ptr)
SBCSbBS512_E3041(ptrA)
SDYSDY_3038(ptr)
SDZAsd1617_04070
SHQA0259_17710
ENCECL_04144
ENLA3UG_18250
ECLGEC036_35650
ECLEECNIH2_18425
ECLNECNIH4_04710
ECLIECNIH5_17205
ECLXLI66_18110
ECLYLI62_19570
ECLZLI64_17015
ECLOENC_30570
EHMAB284_07680
EXFBFV63_17740
ECLAECNIH3_17290
ECLCECR091_17225
EAUDI57_01230
EKBBFV64_18750
ENOECENHK_17955
EECEcWSU1_03620(ptrA)
ELGBH714_21665
ECANCWI88_04405
ERNBFV67_17750
ECLSLI67_019275
ECHGFY206_19785
ESHC1N69_18480
ENTEnt638_3264
EASEntas_3510
ENRH650_10315
ENXNI40_017875
ENFAKI40_0943
LNICWR52_16985
EBGFAI37_11110
ENDA4308_02700
ENZG0034_18420(ptrA)
ENSHWQ15_03480(ptrA)
ENKLOC22_06255(ptrA)
EHUD5067_0004615
EMORL6Y89_17435(ptrA)
ENBELK40_18540
ESAESA_00488
CSKES15_0754(ptr)
CSZCSSP291_02520
CSJCSK29544_01745
CCONAFK62_15900
CDMAFK67_16660
CSIP262_01119
CMJAFK66_003610
CUIAFK65_15670
CMWAFK63_02590
CTUCTU_33700(ptrA)
KPNKPN_03230(ptr)
KPUKP1_4499(ptr)
KPMKPHS_42930
KPPA79E_0880
KPHKPNIH24_07325
KPZKPNIH27_20805
KPVKPNIH29_21575
KPWKPNIH30_21595
KPYKPNIH31_20860
KPGKPNIH32_22455
KPCKPNIH10_21390
KPQKPR0928_20880
KPTVK055_4295
KPOKPN2242_19100
KPRKPR_1803(ptrA)
KPJN559_1008
KPID364_16395
KPAKPNJ1_01014
KPSKPNJ2_01045
KPXPMK1_00711(ptrA_1)
KPBFH42_12110
KPNEKU54_004975
KPNULI86_04985
KPNKBN49_0878(ptrA)
KVAKvar_0840
KPEKPK_0885(ptrA)
KVDKR75_03730
KVQSP68_10275
KOXKOX_01575
KOEA225_4780
KOYJ415_08120
KOMHR38_00030
KMIVW41_19315
KOKKONIH1_24015
KOCAB185_12375
KQUAVR78_25900
EAEEAE_02105
EARCCG33570
KQVB8P98_04900
KLLBJF97_23845
KLWDA718_05340
KARLGL98_04480(ptrA)
KGRJJJ10_05365(ptrA)
KPASLUW96_01675(ptrA)
RORRORB6_23415
RONTE10_22845
RPLNB1209_04765
RAODSD31_04885
RTGNCTC13098_01296(ptrA)
REEelectrica_00886(ptrA)
CROROD_28631(ptrA)
CKOCKO_04186
CFDCFNIH1_01065
CBRAA6J81_21945
CWECO701_03230
CYOCD187_04750
CPOTFOB25_16080
CFQC2U38_20335
CSEDJY391_04385(ptrA)
CAMAF384_15290
CAFAL524_07680
CIFAL515_18710
CFARCI104_20320
CIRC2U53_14660
CIEAN232_09295
CPARCUC49_14555
CTELGBC03_09500
CITZE4Z61_21365
CARSE1B03_21425
EBTEBL_c08090(ptr)
CNTJT31_16390
CEMLH23_01320
CENLH86_01305
CLAPNCTC11466_00689(ptrA)
PGELG71_09205
ESCEntcl_0909
KLEAO703_17045
KSAC813_20035
KORAWR26_04650(ptrA)
KRDA3780_19405
KCOBWI95_05640
KOTEH164_04065
KPSEIP581_18580(ptrA)
KOBHF650_19450(ptrA)
KIENCTC12125_04851(ptrA_1)
KASKATP_08740
KLUK7B04_09690(ptrA)
LAXAPT61_04460
LEIC2U54_23630
LEHC3F35_16250
LEEDVA44_04575
LERGNG29_18495
LEAGNG26_17900
LPNUKQ929_03780(ptrA)
LAZA8A57_16935
LEFLJPFL01_3327
LEWDAI21_09210
BAGEBADSM9389_08680
BFTMNO13_04350(ptrA)
SYMK6K13_19295(ptrA)
METYMRY16398_09980(ptrA)
AHNNCTC12129_04010(ptrA)
ASUBNLZ15_04065(ptrA)
YREHEC60_13695(ptrA)
SGOEA8O29_004825(ptrA)
KINAB182_04345
PDZHHA33_07210(ptrA)
IZHFEM41_00445
PTFPROFFT_A_02210(ptrA)
PLARGII40_00217(ptrA)
PSHISAMEA2665130_2427(ptrA_2)
PSGCG163CM_45260(ptrA)
SCOLKFZ77_15770(ptrA)
PVJLMA04_16305(ptrA)
EBFD782_0900
EBCC2U52_14150
EBUCUC76_20160
PSTSE05_46520
YPEYPO1019(ptr)
YPKy3165(ptr)
YPHYPC_1050(ptr)
YPAYPA_0491
YPNYPN_2982
YPMYP_2882(ptrA)
YPPYPDSF_1696
YPGYpAngola_A3232(ptrA)
YPZYPZ3_0927(ptrA)
YPTA1122_20925
YPDYPD4_0884(ptrA)
YPXYPD8_1187(ptrA)
YPJCH55_3766
YPVBZ15_2553
YPLCH46_4127
YPSYPTB3026(ptrA)
YPOBZ17_3591
YPIYpsIP31758_0990(ptrA)
YPYYPK_1042
YPBYPTS_3148
YPQDJ40_3451
YPUBZ21_2344
YPRBZ20_3135
YPCBZ23_2623
YPFBZ19_2407
YENYE3311(ptrA)
YEPYE105_C0946
YEYY11_40751
YELLC20_01108
YEWCH47_231
YETCH48_765
YEFFORC2_0841
YEEYE5303_05851(ptrA)
YSIBF17_01630
YALAT01_1628
YFRAW19_2293(ptrA)
YINCH53_2693
YKRCH54_3493
YROCH64_1700
YRUBD65_1221
YRBUGYR_06840
YAKACZ76_04790
YMADA391_04700
YHID5F51_17345
YCAF0T03_17180
YMOHRD69_00545(ptrA)
YASN0H69_07715(ptrA)
YKIHRD70_00140(ptrA)
SMARSM39_3418(ptrA)
SMACSMDB11_3208(ptrA)
SMWSMWW4_v1c39360(ptrA)
SPESpro_3815
SRRSerAS9_4004
SRLSOD_c37810(ptrA)
SRYM621_20315
SPLYQ5A_020140(ptrA_2)
SRSSerAS12_4005
SRASerAS13_4005
SMAFD781_3558
SLQM495_19915
SERFL085_09010
SERSSERRSCBI_19060
SFWWN53_01910
SFGAV650_24680
SRZAXX16_1520
SERASer39006_019630
SERQCWC46_19630
SERMCLM71_18670
SQUE4343_21340
SFJSAMEA4384070_3936(ptrA_2)
SOFNCTC11214_02814(ptrA_1)
SSURATE40_016385
SURIJ0X03_04300(ptrA)
SRHZFO014_15545
SENPKHA73_19165(ptrA)
SNEMNLX84_19830(ptrA)
SFOZ042_06145
RAHRahaq_0840
RACEJHW33_20865(ptrA)
RAQRahaq2_0897
RAAQ7S_03950
ROXBV494_21135
RVCJ9880_11815(ptrA)
GQUAWC35_16625
EAMEGXP68_03440(ptrA)
RBADH2866_04040(ptrA)
ECAECA0994(ptrA)
PATREV46_05075
PATOGZ59_10290
PCTPC1_0913
PCCPCC21_009370
PCVBCS7_04825
PWAPecwa_3390
PPARA8F97_01700
PECW5S_3392
PWSA7983_13720
PPOABJK05_21605
PBRAB5S52_16970
PPUJE2566_05230
PARII2D83_17405(ptrA)
PAQUDMB82_0015880(ptrA)
PVZOA04_10460(ptrA)
PPAVLOZ86_17070(ptrA)
PQUIG609_015530(ptrA)
PPOOLW347_05190(ptrA)
PCACOI450_03980(ptrA)
DDDDda3937_00856(ptr)
DZEDd1591_3190
DDCDd586_0905
DZCW909_04775
DSOA4U42_13835
CEDLH89_07420
DFNCVE23_05240
DDQDDI_0751
DAQDAQ1742_03353(ptr)
DICDpoa569_002823
DDADd703_3008
BGJAWC36_13585
BRBEH207_03605
BNGEH206_17695
BIZHC231_18050(ptrA)
SGLSG1974
SODSant_0861(ptrA)
PESSOPEG_3434(ptrA)
SLIGGTU79_04955(ptrA)
EAMEAMY_0710(ptrA)
EAYEAM_2730(ptrA)
ETAETA_27470(ptrA)
EPYEpC_28660(ptrA)
EPREPYR_03101(ptrA)
EBIEbC_35780(ptrA)
ERJEJP617_18700(ptrA)
EGEEM595_2851(ptr)
EPECI789_00410
ERWIGN242_04060
ETPLU633_18560(ptrA)
ERPLJN55_18365(ptrA)
PAMPANA_3108(ptrA)
PLFPANA5342_0924(ptrA)
PAJPAJ_2383(ptrA)
PAQPAGR_g0927
PVAPvag_2494(ptr)
PAGGAL522_19630
PAOPat9b_3125
KLNLH22_06760
PANTPSNIH1_02235
PANPPSNIH2_02185
PAGCBEE12_03030
PSTWDSJ_04965
PALHB1H58_11340
PANSFCN45_15165
PEYEE896_04045
PDISD8B20_13795
PERLAC65_12780(ptrA)
PJIKTJ90_03935(ptrA)
PGZC2E15_17000
PCDC2E16_16885
MINTC7M51_02576(ptrA_1)
MTHIC7M52_00280(ptrA_1)
KPIEN5580_14375(ptrA)
PLUplu0631(ptrA)
PLUMA4R40_03115
PAYPAU_00590(ptrA)
PTTVY86_04745
PAKHB0X70_03655
PLUICE143_03590
PMRPMI2310(ptrA)
PMIBBB2000_2451(ptrA)
PVLAOB99_13510
PVGCRN77_07435
PHAUPH4a_03815
PROTBTA34_13410
PCOLF1325_15010
PCIBF9282_15455
PPEEI6G31_12780(ptrA)
XBOXBJ1_3698(ptrA)
XBVXBW1_4208(ptrA)
XNEXNC1_4037(ptrA)
XNMXNC2_3891(ptrA)
XDOXDD1_3362(ptrA)
XPOXPG1_0267(ptrA)
XHOA9255_18080
XBUHGO23_16875(ptrA)
PSIS70_14890
PSXDR96_320
PSTABGK56_17330
PRGRB151_035780(ptrA_2)
PALACO695_08220
PHEINCTC12003_03087(ptrA)
PRQCYG50_20615
PRJNCTC6933_03187(ptrA)
PVCG3341_15740(ptrA)
PMAGJI723_15905(ptrA)
MMKMU9_909
ANSArsFIN_05880(ptrA)
AETLDL57_03800(ptrA)
MWIMNY66_02600(ptrA)
EICNT01EI_0807
ETRETAE_0709(ptrA)
ETDETAF_0651
ETEETEE_2474
ETCETAC_03425
EDWQY76_16770
EDLAAZ33_03355(ptrA)
EHOA9798_12995
HAVAT03_17605
HPARAL518_08810
OPODSM2777_07640
LPVHYN51_13420
PFQQQ39_14225
PRAGEKN56_03870
LRINCTC12151_00676(ptrA)
HDUHD_0187(ptrA)
HPAAE5Q53_06580
HAPHAPS_1009(ptrA)
HPASJL26_01885
HPAKJT17_10770
GLECJD39_08390
PSKYA6B44_01260
PATLKGI96_06800(ptrA)
MHTD648_3150
MHQD650_24990
MHATB824_23560
MHXMHH_c08610(ptrA)
MHAEF382_09450
MHAMJ450_08380
MHAOJ451_09670
MHALN220_01540
MHAQWC39_12310
MHAYVK67_12315
MVRX781_21600
MVIX808_2070
MVGX874_2160
MVEX875_18760
MANNGM695_01255
MGRAA4G16_08715
MBOSICJ55_03225(ptrA)
MPEGHV560_01330(ptrA)
APLAPL_1883(ptrA)
APJAPJL_1926(ptrA)
APAAPP7_1970(ptrA)
ASIASU2_08060
ASSASU1_08130
AEUACEE_08530
APORDDU33_04125
AIOEXH44_05995
ALIGNCTC10568_02299(ptrA_1)
BTOWQG_2310
BTREF542_19650
BTRHF543_21550
BTRAF544_1880
OOIA6A10_00845
FCLA4G17_03345
SKHSTH12_00051(ptrA_1)
ILOIL2336(ptr)
ILIK734_11760
IPICEW91_01410
IDICWC33_07040
IDTC5610_12155
IABK5X84_11095
LSDEMK97_11080
PTNPTRA_b0517
PSMPSM_B0418
PSEOOM33_19265
PIAPI2015_3437
PPHEPP2015_3366 PP2015_686
PBWD172_018975
PRRAT705_21575
PLZS4054249_21880
PALNB0W48_00380
PPISB1L02_18380 B1L02_19840
PEAPESP_b0612
PSPOPSPO_b0674
PARTPARC_b0522
PTUPTUN_b0584
PNGPNIG_b0480
PTDPTET_b0525
PSENPNC201_17685(ptrA3) PNC201_18680(ptrA4)
PDJD0907_17970
PAGAPAGA_b0682
PCARPC2016_3404
PXIJ5O05_19890
PVBJ5X90_20415
PSAZPA25_33950(ptr)
PDVFFU37_19700
PFLICTT31_20175
GPSC427_4126
LALAT746_17040
PINPing_3480
PSYPCNPT3_01280
CJACJA_3166 CJA_3602
CEBB0D95_11725
CELLCBR65_15660
CEKD0B88_06720
CEGD0C16_13205
GILNHM04_03585 NHM04_11200
RHHE0Z06_13075
KSDKS2013_715
GAPGAPWK_0917
FPPFPB0191_01768
ORBIPMB12_03140(ptrA)
NBLGJV52_09940
NWXCGZ65_04280
DBADbac_3079
DOAAXF15_03410
 » show all
Reference
1  [PMID:3534791]
  Authors
Finch PW, Wilson RE, Brown K, Hickson ID, Emmerson PT
  Title
Complete nucleotide sequence of the Escherichia coli ptr gene encoding protease III.
  Journal
Nucleic Acids Res 14:7695-703 (1986)
DOI:10.1093/nar/14.19.7695
  Sequence
[eco:b2821]
Reference
2  [PMID:3059494]
  Authors
Affholter JA, Fried VA, Roth RA.
  Title
Human insulin-degrading enzyme shares structural and functional homologies with E. coli protease III.
  Journal
Science 242:1415-8 (1988)
DOI:10.1126/science.3059494
Reference
3  [PMID:1570301]
  Authors
Becker AB, Roth RA.
  Title
An unusual active site identified in a family of zinc metalloendopeptidases.
  Journal
Proc Natl Acad Sci U S A 89:3835-9 (1992)
DOI:10.1073/pnas.89.9.3835
Reference
4  [PMID:1733942]
  Authors
Ding L, Becker AB, Suzuki A, Roth RA.
  Title
Comparison of the enzymatic and biochemical properties of human insulin-degrading enzyme and Escherichia coli protease III.
  Journal
J Biol Chem 267:2414-20 (1992)
Reference
5  [PMID:7680857]
  Authors
Anastasi A, Knight CG, Barrett AJ.
  Title
Characterization of the bacterial metalloendopeptidase pitrilysin by use of a continuous fluorescence assay.
  Journal
Biochem J 290 ( Pt 2):601-7 (1993)
DOI:10.1042/bj2900601
Other DBs
ExplorEnz - The Enzyme Database: 3.4.24.55
IUBMB Enzyme Nomenclature: 3.4.24.55
ExPASy - ENZYME nomenclature database: 3.4.24.55
BRENDA, the Enzyme Database: 3.4.24.55
CAS: 81611-78-1

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