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Entry
EC 3.5.1.100                Enzyme                                 

Name
(R)-amidase;
R-stereospecific amidase;
R-amidase
Class
Hydrolases;
Acting on carbon-nitrogen bonds, other than peptide bonds;
In linear amides
BRITE hierarchy
Sysname
(R)-piperazine-2-carboxamide amidohydrolase
Reaction(IUBMB)
(1) (R)-piperazine-2-carboxamide + H2O = (R)-piperazine-2-carboxylate + NH3 [RN:R09647];
(2) beta-alaninamide + H2O = beta-alanine + NH3 [RN:R09648]
Reaction(KEGG)
Substrate
(R)-piperazine-2-carboxamide [CPD:C19778];
H2O [CPD:C00001];
beta-alaninamide [CPD:C19779]
Product
(R)-piperazine-2-carboxylate [CPD:C19780];
NH3 [CPD:C00014];
beta-alanine [CPD:C00099]
Comment
In addition (R)-piperidine-3-carboxamide is hydrolysed to (R)-piperidine-3-carboxylic acid and NH3, and (R)-N-tert-butylpiperazine-2-carboxamide is hydrolysed to (R)-piperazine-2-carboxylic acid and tert-butylamine with lower activity. The enzyme does not act on the other amide substrates which are hydrolysed by EC 3.5.1.4 (amidase).
History
EC 3.5.1.100 created 2009, modified 2011
Orthology
K18540  (R)-amidase
Genes
SLQ: M495_09540
SFW: WN53_23135
SFG: AV650_18940
PAE: PA3598
PAEV: N297_3722(ramA)
PAEI: N296_3722(ramA)
PAU: PA14_17730
PAP: PSPA7_1543
PAG: PLES_14371
PAF: PAM18_1387
PAEP: PA1S_07420
PAEM: U769_06900
PAEL: T223_07135
PAEG: AI22_26485
PAEC: M802_3719(ramA)
PAEO: M801_3587(ramA)
PCQ: PcP3B5_21760(ramA_1)
PPU: PP_3846(ramA)
PPT: PPS_2644
PPUH: B479_12805
PPUD: DW66_2904
PMON: X969_12555
PMOT: X970_12200
PFL: PFL_4898
PPRC: PFLCHA0_c48790(ramA)
PPRO: PPC_4907
PMAN: OU5_4712
PEN: PSEEN3582
PKC: PKB_3494(ramA)
PSEM: TO66_25495
PANR: A7J50_1111
PSET: THL1_1666
PSIL: PMA3_19055
CSA: Csal_0061
TAU: Tola_1341
PSE: NH8B_1826
BVE: AK36_2221(ramA)
BCN: Bcen_4372
BCJ: BCAL1380(ramA)
BCEN: DM39_1273(ramA)
BCEO: I35_1275(ramA)
BAM: Bamb_1638
BMU: Bmul_5017
BMUL: NP80_1792(ramA)
BCT: GEM_2060
BCED: DM42_329(ramA)
BDL: AK34_1396(ramA)
BCON: NL30_08850
BTEI: WS51_07825
BSEM: WJ12_06730
BPSL: WS57_25140
BMEC: WJ16_06595
BGU: KS03_4414(ramA)
BGO: BM43_4600(ramA)
BUK: MYA_1509
BFN: OI25_201
 » show all
Taxonomy
Reference
1  [PMID:15066183]
  Authors
Komeda H, Harada H, Washika S, Sakamoto T, Ueda M, Asano Y
  Title
A novel R-stereoselective amidase from Pseudomonas sp. MCI3434 acting on piperazine-2-tert-butylcarboxamide.
  Journal
Eur J Biochem 271:1580-90 (2004)
DOI:10.1111/j.1432-1033.2004.04069.x
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 3.5.1.100
IUBMB Enzyme Nomenclature: 3.5.1.100
ExPASy - ENZYME nomenclature database: 3.5.1.100
BRENDA, the Enzyme Database: 3.5.1.100

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