KEGG   ENZYME: 3.5.1.103
Entry
EC 3.5.1.103                Enzyme                                 

Name
N-acetyl-1-D-myo-inositol-2-amino-2-deoxy-alpha-D-glucopyranoside deacetylase;
MshB
Class
Hydrolases;
Acting on carbon-nitrogen bonds, other than peptide bonds;
In linear amides
Sysname
1-(2-acetamido-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol acetylhydrolase
Reaction(IUBMB)
1-O-(2-acetamido-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + H2O = 1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + acetate [RN:R09651]
Reaction(KEGG)
R09651
Substrate
1-O-(2-acetamido-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol;
H2O [CPD:C00001]
Product
1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol [CPD:C19702];
acetate [CPD:C00033]
Comment
This enzyme is considered the key enzyme and rate limiting step in the mycothiol biosynthesis pathway [1]. In addition to acetylase activity, the enzyme possesses weak activity of EC 3.5.1.115, mycothiol S-conjugate amidase, and shares sequence similarity with that enzyme [2]. The enzyme requires a divalent transition metal ion for activity, believed to be Zn2+ [3].
History
EC 3.5.1.103 created 2010
Orthology
K15525  N-acetyl-1-D-myo-inositol-2-amino-2-deoxy-alpha-D-glucopyranoside deacetylase
Genes
RHD: R2APBS1_1456
RGL: CS053_09200
DJI: CH75_05360
DJA: HY57_10920
DTX: ATSB10_05710
LRZ: BJI69_21410
LPY: FIV34_13470
LLU: AKJ09_05353
HOH: Hoch_4210
AEP: AMC99_02306
BTS: Btus_1144
SAY: TPY_3498
MTU: Rv1170(mshB)
MTV: RVBD_1170
MTC: MT1207
MRA: MRA_1181(mshB)
MTUR: CFBS_1249(mshB)
MTO: MTCTRI2_1202(mshB)
MTD: UDA_1170(mshB)
MTN: ERDMAN_1313(mshB)
MTUC: J113_08195
MTUE: J114_06325
MTUH: I917_08310
MTUL: TBHG_01154
MTUT: HKBT1_1247(mshB)
MTUU: HKBT2_1253(mshB)
MTQ: HKBS1_1251(mshB)
MBO: BQ2027_MB1203(mshB)
MBB: BCG_1233(mshB)
MBT: JTY_1206(mshB)
MBM: BCGMEX_1205(mshB)
MBX: BCGT_1002
MAF: MAF_11890(mshB)
MMIC: RN08_1314
MCE: MCAN_11811(mshB)
MCQ: BN44_11308(mshB)
MCV: BN43_30237(mshB)
MCX: BN42_21035(mshB)
MCZ: BN45_30230(mshB)
MLE: ML1495
MLB: MLBr01495
MPA: MAP_2613c
MAO: MAP4_1205
MAVU: RE97_05965
MAV: MAV_1310
MIT: OCO_12210
MIA: OCU_12170
MID: MIP_01950
MYO: OEM_12360
MIR: OCQ_12230
MMAL: CKJ54_05930(mshB)
MLP: MLM_1241
MMAN: MMAN_27790(mshB)
MUL: MUL_1017(mshB)
MMC: Mmcs_4034
MKM: Mkms_4109
MJL: Mjls_4264
MMI: MMAR_4283(mshB)
MMAE: MMARE11_40980(mshB)
MMM: W7S_05960
MLI: MULP_04469(mshB)
MHAD: B586_07400
MSHG: MSG_03836(mshB)
MFJ: MFLOJ_48310(mshB)
MSTO: MSTO_52220(mshB)
MSIM: MSIM_38250(mshB)
MSAK: MSAS_21190(mshB)
MGRO: FZ046_26060(mshB)
MXE: MYXE_14900(mshB)
MNV: MNVI_05240(mshB)
MPAG: C0J29_23435(mshB)
MNM: MNVM_06760(mshB)
MGOR: H0P51_21625(mshB)
MCOO: MCOO_19530(mshB)
MBAI: MB901379_03736(mshB_1)
MSEO: MSEO_26140(mshB)
MPSE: MPSD_44100(mshB)
MSHO: MSHO_14800(mshB)
MHEK: JMUB5695_01418(mshB_1)
MGAU: MGALJ_29030(mshB)
MLJ: MLAC_07760(mshB)
MBRD: MBRA_28560(mshB)
MSHJ: MSHI_03780(mshB)
MKR: MKOR_00470(mshB)
MDF: K0O62_21970(mshB)
MMAM: K3U93_18460(mshB)
MSG: MSMEI_5001(mshB)
MVA: Mvan_4535
MGI: Mflv_2165
MFT: XA26_45410(mshB)
MPHL: MPHLCCUG_04078(mshB)
MVQ: MYVA_4310
MTHN: 4412656_03418(mshB)
MHAS: MHAS_02882(mshB)
MDU: MDUV_01930(mshB)
MCHT: MCHIJ_26360(mshB)
MDR: MDOR_28390(mshB)
MAUU: NCTC10437_04268(mshB_1)
MMAG: MMAD_41950(mshB)
MMOR: MMOR_20520(mshB)
MFX: MFAL_38610(mshB)
MAIC: MAIC_42110(mshB)
MIJ: MINS_37750(mshB)
MALV: MALV_49360(mshB)
MTY: MTOK_03580(mshB)
MPSC: MPSYJ_23230(mshB)
MARZ: MARA_55050(mshB)
MGAD: MGAD_53390(mshB)
MHEV: MHEL_08690(mshB)
MSAR: MSAR_27750(mshB)
MANY: MANY_25650(mshB)
MAUB: MAUB_44370(mshB)
MPOF: MPOR_46010(mshB)
MPHU: MPHO_24990(mshB)
MMUC: C1S78_005890(mshB)
MMAT: MMAGJ_04460(mshB)
MBOK: MBOE_28310(mshB)
MAB: MAB_1316
MABB: MASS_1315
MCHE: BB28_06490
MSTE: MSTE_01284(mshB)
MSAL: DSM43276_01182(mshB_1)
MJD: JDM601_1159(mshB)
MTER: 4434518_01109(mshB)
MMIN: MMIN_27910(mshB)
MHIB: MHIB_06810(mshB)
ASD: AS9A_3372(mshB)
CGL: Cgl1100(Cgl1100)
CGB: cg1250
CGU: WA5_1055(MshB)
CGT: cgR_1183
CGM: cgp_1250(mshB)
CGJ: AR0_06000
CEF: CE1158
CDI: DIP0971
CDP: CD241_0879(mshB)
CDH: CDB402_0847(mshB)
CDT: CDHC01_0879(mshB)
CDE: CDHC02_0878(mshB)
CDR: CDHC03_0875(mshB)
CDA: CDHC04_0885(mshB)
CDZ: CD31A_0977(mshB)
CDB: CDBH8_0939(mshB)
CDS: CDC7B_0884(mshB)
CDD: CDCE8392_0875(mshB)
CDW: CDPW8_0935(mshB)
CDV: CDVA01_0842(mshB)
CDIP: ERS451417_00874(mshB)
CJK: jk1397
CUR: cu0660
CUA: CU7111_0649(mshB)
CAR: cauri_0978(mshB)
CPL: Cp3995_0781(mshB)
CPG: CP316_04005(mshB)
CPP: CpP54B96_0780(mshB)
CPK: CP1002_09270(mshB)
CPQ: CPC231_03865(mshB)
CPX: CPI19_07655(mshB)
CPZ: CpPAT10_0768(mshB)
COR: Cp267_0803(mshB)
COP: CP31_04235(mshB)
COD: Cp106_0753(mshB)
COS: Cp4202_0759(mshB)
COI: CPCIP5297_04025(mshB)
COE: CP258_04020(mshB)
COU: CP162_07250(mshB)
CPSE: CPTA_01324
CPSU: CPTB_00208
CPSF: CPTC_01314
CRD: CRES_1472(mshB)
CUN: Cul210932_0856(mshB)
CUS: CulFRC11_0822(mshB)
CUQ: Cul210931_0819(mshB)
CUZ: Cul05146_0878(mshB)
CUJ: CUL131002_0834(mshB)
CVA: CVAR_1875(mshB)
CTER: A606_07630
CGY: CGLY_06475(mshB)
COA: DR71_1804(mshB)
CSX: CSING_05390(mshB)
CMQ: B840_04480(mshB)
CKU: UL82_07825(mshB)
CCJ: UL81_04480(mshB)
CMV: CMUST_05905(mshB)
CEI: CEPID_04680(mshB)
CTED: CTEST_04805(mshB)
CUT: CUTER_04055(mshB)
CDX: CDES_05320(mshB)
CSP: WM42_0392
CPHO: CPHO_07915
CGV: CGLAU_04845(mshB)
CAQU: CAQU_04700
CAMG: CAMM_08565
CMIN: NCTC10288_01565(mshB)
CPEG: CPELA_07030(mshB)
CXE: FOB82_09280(mshB)
CEE: CENDO_04360(mshB)
CSAN: E3227_10815(mshB)
CGK: CGERO_04150(mshB)
CRL: NCTC7448_01639(mshB_2)
CCHO: CCHOA_04025(mshB)
CPRE: Csp1_09880(mshB)
CPSO: CPPEL_07255(mshB)
CSUR: N24_1219(mshB)
CCYS: SAMEA4530656_1445(mshB_1)
CMAT: HBA49_11435(mshB)
CYZ: C3B44_07225(mshB)
CANS: GP473_06305(mshB)
CLIA: C3E79_04170(mshB)
CAMC: I6I65_00420(mshB)
CUO: CUROG_07035(mshB)
NFA: NFA_47570(mshB)
NFR: ERS450000_05171(mshB_2)
NCY: NOCYR_4504(mshB)
NNO: NONO_c35200(mshB1) NONO_c64660(mshB2)
NSR: NS506_01888(mshB)
NTP: CRH09_33565(mshB)
NOZ: DMB37_14620(mshB)
NOD: FOH10_02825(mshB)
NAH: F5544_37950(mshB)
NAD: NCTC11293_06084(mshB_1)
NWL: NWFMUON74_12020(mshB)
NIE: KV110_34980(mshB)
NTC: KHQ06_12060(mshB)
RER: RER_41900(mshB)
REY: O5Y_19590
RQI: C1M55_20680(mshB)
ROP: ROP_59960(mshB)
REQ: REQ_14110(mshB)
RBY: CEJ39_09000(mshB)
RHB: NY08_5012
RFA: A3L23_03070(mshB_2)
RHS: A3Q41_00258(mshB_1)
RRZ: CS378_23315(mshB)
RHU: A3Q40_02336(mshB)
RRT: 4535765_01322(mshB_2)
RCR: NCTC10994_01480(mshB_1)
RTM: G4H71_03860(mshB)
RKO: JWS14_32420(mshB)
RGO: KYT97_28100(mshB)
GRU: GCWB2_10840(mca3) GCWB2_17315(mshB2)
GOM: D7316_01227(mshB_1) D7316_05359(mshB_2)
SPIN: KV203_14940(mshB)
TPR: Tpau_1146
SRT: Srot_0833
DIT: C3V38_15820(mshB)
DIZ: CT688_09935(mshB)
TOY: FO059_07595(mshB)
SCO: SCO5126(SC9E12.11)
SALB: XNR_1686
SMA: SAVERM_3138(mshB)
SGR: SGR_2405(mshB)
SMAL: SMALA_5078
SSOI: I1A49_29080(mshB)
SCT: SCAT_3982
SFA: Sfla_2168
SBH: SBI_04101
SDV: BN159_3280(mshB)
SALS: SLNWT_2192
STRP: F750_4644(mshB)
SLV: SLIV_12750(mshB)
SGU: SGLAU_21965(mshB)
STRE: GZL_03583
SLD: T261_2938
STRM: M444_22410
SAMB: SAM23877_4908(mshB)
SPRI: SPRI_2752
SRW: TUE45_01129(mca_1) TUE45_05617(mshB_2)
SLE: sle_26120(sle_26120)
SRN: A4G23_03743(mshB)
SKY: D0C37_10185(mshB)
SGM: GCM10017557_27750(mshB)
SCAD: DN051_14765(mshB)
STRD: NI25_13515
SALJ: SMD11_2437
SLX: SLAV_03235(mca2) SLAV_14000(mshB2)
SFK: KY5_5231
SNZ: DC008_22690(mshB)
SGE: DWG14_03026(mshB_2)
SDX: C4B68_13935(mshB)
SGD: ELQ87_13805(mshB)
SQZ: FQU76_22735(mshB)
SCYA: EJ357_30270(mshB)
SAST: CD934_11260(mshB)
SNQ: CP978_21970(mshB)
STIR: DDW44_19920(mshB)
SKA: CP970_15005(mshB)
SGZ: C0216_04560(mshB)
SVN: CP980_12035(mshB)
SHAW: CEB94_26815(mshB)
SRK: FGW37_21325(mshB)
SFIC: EIZ62_11175(mshB)
SGAL: CP966_23805(mshB)
SVR: CP971_12885(mshB)
SPAD: DVK44_23170(mshB)
SFY: GFH48_15465(mshB)
SGF: HEP81_02901(mshB_1) HEP81_07330(mshB_2)
SSEO: D0Z67_19030(mshB)
SBY: H7H31_10805(mshB)
SRIM: CP984_13825(mshB)
SSUB: CP968_12195(mshB)
SPHV: F9278_32705(mshB)
SCIN: CP977_22535(mshB)
SFUG: CNQ36_23200(mshB)
SCHA: CP983_15125(mshB)
SGX: H4W23_26320(mshB)
SBAT: G4Z16_10410(mshB)
SSPB: CP982_27190(mshB)
SNF: JYK04_05479(mshB_2)
SPLA: CP981_25445(mshB)
SBRO: GQF42_28160(mshB)
STUI: GCM10017668_46500(mshB)
SATA: C5746_27115(mshB)
SCHF: IPT68_23585(mshB)
SHUN: DWB77_02791(mshB_2)
SHAR: HUT13_18340(mshB)
SCYG: S1361_25355(mshB1)
SFEU: IM697_38235(mshB)
SDW: K7C20_24190(mshB)
SVD: CP969_25055(mshB)
KSK: KSE_48330(mshB)
STRI: C7M71_019560(mshB)
TWH: TWT_798
TWS: TW808
LXL: KDY119_01100(mshB)
CMI: CMM_2174
CMS: CMS2206
CMC: CMN_02142
ARM: ART_1903
KRH: KRH_08220(mshB)
KVR: CIB50_0002336(mshB_2)
BCV: Bcav_1124
JDE: Jden_0794
LMOI: VV02_20450
XCE: Xcel_0963
IDO: I598_1357(mshB)
CFL: Cfla_0988
CFI: Celf_2899
CELZ: E5225_13100(mshB)
JLI: EXU32_11505(mshB)
JME: EEW87_011430(mshB)
TEH: GKE56_10045(mshB)
SERJ: SGUI_2990
SERW: FY030_11230(mshB)
BLIN: BLSMQ_1667
DCO: SAMEA4475696_2002(mshB_2)
AUS: IPK37_13700(mshB)
PFRE: RM25_2107
PAUS: NCTC13651_00189(mshB_1)
MPH: MLP_43290(mshB)
MIK: FOE78_19800(mshB)
MICG: GJV80_12255(mshB)
TDF: H9L22_16175(mshB)
TLA: TLA_TLA_01778(mshB_2)
NCA: Noca_1157
NDK: I601_2148(mshB_1)
NOY: EXE57_16420(mshB)
NOI: FCL41_03740(mshB)
NOO: FE634_04265(mshB)
NDP: E2C04_04515(mshB)
NSN: EXE58_02670(mshB)
NBE: Back2_10170(mshB)
NANO: G5V58_17065(mshB)
NMES: H9L09_14225(mshB)
NARO: CFH99_06800(mshB)
NPI: G7071_00630(mshB)
PSIM: KR76_20790
AEZ: C3E78_12970(mshB)
AEB: C6I20_11450(mshB)
AEF: GEV26_13015(mshB)
MUZ: H4N58_04810(mshB)
KQI: F1D05_11905(mshB)
TFU: Tfu_0486
NDA: Ndas_3908
NAL: B005_1085(mshB)
NGV: CDO52_07605(mshB)
STRR: EKD16_03800(mshB1)
TCU: Tcur_1131
ACTW: F7P10_30835(mshB)
SRO: Sros_8449
NOW: GBF35_44425(mshB)
NCX: Nocox_36655(mshB2)
TBI: Tbis_3036
ACE: Acel_1868
NML: Namu_4309
NAK: EH165_04035(mshB)
GOB: Gobs_1084
MMAR: MODMU_1135
KRA: Krad_1134
SACE: GIY23_04250(mshB)
SACG: FDZ84_16760(mshB) FDZ84_20150(mshB)
SACC: EYD13_17260(mshB2)
AMD: AMED_8023
AMN: RAM_41220
AMM: AMES_7904
AMZ: B737_7904
AOI: AORI_6834
AJA: AJAP_04940(mshB)
AMYC: CU254_32665(mshB)
AMYY: YIM_05430(mshB1)
AORI: SD37_06485
PDX: Psed_1005
PSEA: WY02_21210
PSEE: FRP1_22120
PSEH: XF36_20365
PAUT: Pdca_11220(mshB)
PBRO: HOP40_03220(mshB)
APRE: CNX65_03790(mshB)
AMI: Amir_0766
SESP: BN6_08510(mshB)
SSYI: EKG83_04060(mshB)
ACTI: UA75_04500
ACAD: UA74_04395
AHG: AHOG_04250(mshB1)
ACTA: C1701_07565(mshB)
ALO: CRK61671
SAQ: Sare_4145
MIL: ML5_2969
MTUA: CSH63_30790(mshB)
MICH: FJK98_27920(mshB)
MTEM: GCE86_21225(mshB)
MCAB: HXZ27_26715(mshB)
MSAG: GCM10017556_15750(mshB)
MCRA: ID554_19955(mshB)
ASE: ACPL_7649(mshB)
AMS: AMIS_73690(mshB)
ACTN: L083_7369(mshB)
AFS: AFR_38780
ACTS: ACWT_7519
AIH: Aiant_34930(mshB)
PLAB: C6361_36360(mshB)
PLAT: C6W10_10260(mshB)
PFLA: Pflav_062850(mshB)
PSUU: Psuf_024710(mshB)
VER: HUT12_27590(mshB)
ATL: Athai_51560(mshB)
ASER: Asera_23270(mshB)
PRY: Prubr_34750(mshB)
DVC: Dvina_05170(mshB)
SNA: Snas_5506
NAV: JQS30_12610(mshB)
GLY: K3N28_07730(mshB)
AEY: CDG81_17765(mshB)
ABAM: B1s21122_01305(mshB)
ERZ: ER308_17420(mshB)
DEW: DGWBC_0129(mshB)
DFO: Dform_00451(mshB)
RCA: Rcas_0528
ATM: ANT_14070
DGE: Dgeo_1021
DFC: DFI_15285
MRB: Mrub_2816
ABAC: LuPra_04102(mshB)
 » show all
Reference
1  [PMID:12724395]
  Authors
Rawat M, Kovacevic S, Billman-Jacobe H, Av-Gay Y
  Title
Inactivation of mshB, a key gene in the mycothiol biosynthesis pathway in Mycobacterium smegmatis.
  Journal
Microbiology 149:1341-9 (2003)
DOI:10.1099/mic.0.26084-0
Reference
2  [PMID:11092856]
  Authors
Newton GL, Av-Gay Y, Fahey RC
  Title
N-Acetyl-1-D-myo-inosityl-2-amino-2-deoxy-alpha-D-glucopyranoside deacetylase (MshB) is a key enzyme in mycothiol biosynthesis.
  Journal
J Bacteriol 182:6958-63 (2000)
DOI:10.1128/JB.182.24.6958-6963.2000
  Sequence
[mtu:Rv1170] [msm:MSMEG_5129]
Reference
3  [PMID:12958317]
  Authors
Maynes JT, Garen C, Cherney MM, Newton G, Arad D, Av-Gay Y, Fahey RC, James MN
  Title
The crystal structure of 1-D-myo-inosityl 2-acetamido-2-deoxy-alpha-D-glucopyranoside deacetylase (MshB) from Mycobacterium tuberculosis reveals a zinc hydrolase with a lactate dehydrogenase fold.
  Journal
J Biol Chem 278:47166-70 (2003)
DOI:10.1074/jbc.M308914200
  Sequence
[mtu:Rv1170]
Other DBs
ExplorEnz - The Enzyme Database: 3.5.1.103
IUBMB Enzyme Nomenclature: 3.5.1.103
ExPASy - ENZYME nomenclature database: 3.5.1.103
BRENDA, the Enzyme Database: 3.5.1.103

DBGET integrated database retrieval system