KEGG   ENZYME: 3.5.1.117
Entry
EC 3.5.1.117                Enzyme                                 

Name
6-aminohexanoate-oligomer endohydrolase;
endo-type 6-aminohexanoate oligomer hydrolase;
Ahx endo-type-oligomer hydrolase;
6-aminohexanoate oligomer hydrolase;
Ahx-oligomer hydrolase;
nylon hydrolase;
nylon-oligomer hydrolase;
NylC;
nylon-6 hydrolase (ambiguous)
Class
Hydrolases;
Acting on carbon-nitrogen bonds, other than peptide bonds;
In linear amides
Sysname
6-aminohexanoate oligomer endoamidohydrolase
Reaction(IUBMB)
[N-(6-aminohexanoyl)]n + H2O = [N-(6-aminohexanoyl)]n-x + [N-(6-aminohexanoyl)]x [RN:R10976]
Reaction(KEGG)
R10976;
(other) R10978 R10979
Substrate
[N-(6-aminohexanoyl)]n [CPD:C20988];
H2O [CPD:C00001]
Product
[N-(6-aminohexanoyl)]n-x;
[N-(6-aminohexanoyl)]x
Comment
The enzyme is involved in degradation of nylon-6 oligomers. It degrades linear or cyclic oligomers of poly(6-aminohexanoate) with a degree of polymerization greater than three (n > 3) by endo-type cleavage, to oligomers of a length of two or more (2 <= x < n). It shows negligible activity with N-(6-aminohexanoyl)-6-aminohexanoate (cf. EC 3.5.1.46, 6-aminohexanoate-oligomer exo hydrolase) or with 1,8-diazacyclotetradecane-2,9-dione (cf. EC 3.5.2.12, 6-aminohexanoate-cyclic-dimer hydrolase).
History
EC 3.5.1.117 created 2014
Pathway
ec00930  Caprolactam degradation
ec01120  Microbial metabolism in diverse environments
Orthology
K19963  6-aminohexanoate-oligomer endohydrolase
Genes
MICR: BMW26_04430
LTR: EVS81_05135
AGG: C1N71_00300
ACTW: F7P10_35555
NOA: BKM31_31370
AMYY: YIM_16760
PMAD: BAY61_11955
MICH: FJK98_13105
MCAB: HXZ27_01495
Reference
1  [PMID:8285701]
  Authors
Kakudo S, Negoro S, Urabe I, Okada H
  Title
Nylon oligomer degradation gene, nylC, on plasmid pOAD2 from a Flavobacterium strain encodes endo-type 6-aminohexanoate oligomer hydrolase: purification and characterization of the nylC gene product.
  Journal
Appl Environ Microbiol 59:3978-80 (1993)
DOI:10.1128/AEM.59.11.3978-3980.1993
Reference
2  [PMID:17827307]
  Authors
Yasuhira K, Tanaka Y, Shibata H, Kawashima Y, Ohara A, Kato D, Takeo M, Negoro S
  Title
6-Aminohexanoate oligomer hydrolases from the alkalophilic bacteria Agromyces sp. strain KY5R and Kocuria sp. strain KY2.
  Journal
Appl Environ Microbiol 73:7099-102 (2007)
DOI:10.1128/AEM.00777-07
Reference
3  [PMID:22187439]
  Authors
Negoro S, Shibata N, Tanaka Y, Yasuhira K, Shibata H, Hashimoto H, Lee YH, Oshima S, Santa R, Oshima S, Mochiji K, Goto Y, Ikegami T, Nagai K, Kato D, Takeo M, Higuchi Y
  Title
Three-dimensional structure of nylon hydrolase and mechanism of nylon-6 hydrolysis.
  Journal
J Biol Chem 287:5079-90 (2012)
DOI:10.1074/jbc.M111.321992
Other DBs
ExplorEnz - The Enzyme Database: 3.5.1.117
IUBMB Enzyme Nomenclature: 3.5.1.117
ExPASy - ENZYME nomenclature database: 3.5.1.117
BRENDA, the Enzyme Database: 3.5.1.117

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