KEGG   ENZYME: 3.5.1.119Help
Entry
EC 3.5.1.119                Enzyme                                 

Name
Pup amidohydrolase;
dop (gene name);
Pup deamidase;
depupylase/deamidase;
DPUP;
depupylase
Class
Hydrolases;
Acting on carbon-nitrogen bonds, other than peptide bonds;
In linear amides
BRITE hierarchy
Sysname
[prokaryotic ubiquitin-like protein]-L-glutamine amidohydrolase
Reaction(IUBMB)
[prokaryotic ubiquitin-like protein]-L-glutamine + H2O = [prokaryotic ubiquitin-like protein]-L-glutamate + NH3 [RN:R11209]
Reaction(KEGG)
R11209;
(other) R11208
Show
Substrate
[prokaryotic ubiquitin-like protein]-L-glutamine [CPD:C21179];
H2O [CPD:C00001]
Product
[prokaryotic ubiquitin-like protein]-L-glutamate [CPD:C21177];
NH3 [CPD:C00014]
Comment
The enzyme has been characterized from the bacterium Mycobacterium tuberculosis. It catalyses the hydrolysis of the amido group of the C-terminal glutamine of prokaryotic ubiquitin-like protein (Pup), thus activating it for ligation to target proteins, a process catalysed by EC 6.3.1.19, prokaryotic ubiquitin-like protein ligase. The reaction requires ATP as cofactor but not its hydrolysis. The enzyme also catalyses the hydrolytic cleavage of the bond formed by the ligase, between an epsilon-amino group of a lysine residue of the target protein and the gamma-carboxylate of the C-terminal glutamate of the prokaryotic ubiquitin-like protein.
History
EC 3.5.1.119 created 2015
Orthology
K20814  Pup amidohydrolase
Genes
MTU: Rv2112c(dop)
MTV: RVBD_2112c
MTC: MT2172
MRA: MRA_2127
MTF: TBFG_12144
MTB: TBMG_01868
MTK: TBSG_01881
MTZ: TBXG_001852
MTG: MRGA327_13005
MTI: MRGA423_13140
MTUR: CFBS_2237
MTD: UDA_2112c
MTUB: MT7199_2142(dop)
MTUC: J113_14635
MTUE: J114_11320
MTUL: TBHG_02066
MTUT: HKBT1_2229
MTUU: HKBT2_2230
MBO: BQ2027_MB2136C(dop)
MBB: BCG_2129c
MBT: JTY_2123
MBX: BCGT_1932
MAF: MAF_21240
MMIC: RN08_2340
MCQ: BN44_50039(dop)
MCV: BN43_31328(dop)
MCX: BN42_40023(dop)
MCZ: BN45_50433(dop)
MLE: ML1320
MLB: MLBr01320
MPA: MAP_1837c
MAO: MAP4_1991
MAVI: RC58_09935
MAVU: RE97_09945
MIT: OCO_24050
MIA: OCU_23930
MID: MIP_03321
MYO: OEM_22480
MIR: OCQ_22580
MLP: MLM_2192
MUL: MUL_2333
MMC: Mmcs_3131
MKM: Mkms_3193
MJL: Mjls_3143
MMI: MMAR_3087
MMM: W7S_11595
MHAD: B586_10640
MSHG: MSG_02977
MVA: Mvan_3451
MGI: Mflv_3075
MPHL: MPHLCCUG_02722(dop)
MVQ: MYVA_3371
MTHN: 4412656_01936(dop)
MHAS: MHAS_01599(dop)
MAB: MAB_2170
MABB: MASS_2097
MCHE: BB28_10920
MSTE: MSTE_02109
MSAL: DSM43276_01885(dop)
MTER: 4434518_01735(dop)
ASD: AS9A_2007
CGL: NCgl1439(Cgl1496)
CGB: cg1690
CGU: WA5_1439
CGT: cgR_1556
CGM: cgp_1690(pafA1)
CGJ: AR0_08015
CEF: CE1625
CDI: DIP1247
CDP: CD241_1177(pafA1)
CDH: CDB402_1156(pafA1)
CDT: CDHC01_1176(pafA1)
CDE: CDHC02_1155(pafA1)
CDR: CDHC03_1150(pafA1)
CDA: CDHC04_1160(pafA1)
CDZ: CD31A_1258(pafA1)
CDB: CDBH8_1225(pafA1)
CDS: CDC7B_1241(pafA1)
CDD: CDCE8392_1148(pafA1)
CDW: CDPW8_1226(pafA1)
CDV: CDVA01_1117(pafA1)
CDIP: ERS451417_01247(pafA1)
CJK: jk0947
CUR: cu1041
CUA: CU7111_1023(pafA1)
CKP: ckrop_0912(pafA1)
CPU: cpfrc_01039(pafA1)
CPL: Cp3995_1058(pafA1)
CPG: Cp316_1079(pafA1)
CPP: CpP54B96_1054(pafA1)
CPK: Cp1002_1035(pafA1)
CPQ: CpC231_1034(pafA1)
CPX: CpI19_1040(pafA1)
CPZ: CpPAT10_1034(pafA1)
COR: Cp267_1083(pafA1)
COP: Cp31_1044(pafA1)
COD: Cp106_1017(pafA1)
COS: Cp4202_1027(pafA1)
COI: CpCIP5297_1052(pafA1)
COE: Cp258_1050(pafA1)
COU: Cp162_1033(pafA1)
CPSE: CPTA_01606
CPSU: CPTB_02157
CPSF: CPTC_01597
CRD: CRES_1025(pafA1)
CUL: CULC22_01110(pafA1)
CUC: CULC809_01095(pafA1)
CUN: Cul210932_1151(pafA1)
CUS: CulFRC11_1119(pafA1)
CUQ: Cul210931_1101(pafA1)
CUZ: Cul05146_1175(pafA1)
CUJ: CUL131002_1133c(pafA1)
CVA: CVAR_1423
CTER: A606_05530
CGY: CGLY_08730(dop)
COA: DR71_62
CSX: CSING_06950(dop)
CMQ: B840_06280(dop)
CCJ: UL81_06000(dop)
CMV: CMUST_08020(dop)
CEI: CEPID_06390(dop)
CTED: CTEST_06620(dop)
CUT: CUTER_05440(dop)
CDX: CDES_07225(dop)
CSP: WM42_0093
CPHO: CPHO_06085
CGV: CGLAU_06385(dop)
CAMG: CAMM_06730
CMIN: NCTC10288_01262(dop)
CPEG: CPELA_05575(dop)
NFA: NFA_31740
NFR: ERS450000_00898(dop)
RER: RER_31760
REY: O5Y_14590
ROP: ROP_05840
REQ: REQ_23260
RHB: NY08_1012
RFA: A3L23_04262(dop)
RHS: A3Q41_04004(dop)
RHU: A3Q40_01722(dop)
RRT: 4535765_02613(dop)
GBR: Gbro_2461
GPO: GPOL_c23950(dop)
GOR: KTR9_2370
GRU: GCWB2_13030(dop)
GOM: D7316_01001(dop)
TPR: Tpau_2155
SRT: Srot_1599
SCO: SCO1647(SCI41.30c)
SALB: XNR_5181
SGR: SGR_5857
SGB: WQO_05995
SCT: SCAT_5171
SFA: Sfla_5189
SBH: SBI_08479
SHY: SHJG_3083
SVE: SVEN_1239
SDV: BN159_6936(dop)
SALS: SLNWT_6256
STRP: F750_1444(pafA)
SFI: SFUL_1164
SALU: DC74_2116
SALL: SAZ_11145
SLV: SLIV_29510(dop)
SGU: SGLAU_06805(dop)
STRE: GZL_07022
SLD: T261_6597
STRM: M444_08570
SAMB: SAM23877_1702(dop)
SPRI: SPRI_5909
SRW: TUE45_02073(dop)
SLE: sle_54710(sle_54710)
SRN: A4G23_00880(dop)
SNR: SNOUR_32180(dop)
STRD: NI25_30955
SMAL: SMALA_1434
SLAU: SLA_1182
SALF: SMD44_01701(pafA)
SALJ: SMD11_5377(pafA)
SLX: SLAV_29275(dop)
SFK: KY5_1449c
SGE: DWG14_06828(dop)
KSK: KSE_66610
ART: Arth_2174
ARM: ART_0671
ARX: ARZXY2_1805(pafA)
AAU: AAur_2175
ACH: Achl_1917
KRH: KRH_13870
BCV: Bcav_2240
JDE: Jden_1202
LMOI: VV02_15930
XCE: Xcel_1486
IDO: I598_0772(dop)
CFL: Cfla_2003
CFI: Celf_1790
SERJ: SGUI_0333
BLIN: BLSMQ_2083
MPH: MLP_26140
NCA: Noca_2643
NDK: I601_3317(dop)
KFL: Kfla_3224
PSIM: KR76_13395
MGG: MPLG2_1405(dop)
TFU: Tfu_1796
NDA: Ndas_1488
NAL: B005_4097
STRR: EKD16_11895(dop)
TCU: Tcur_2308
SRO: Sros_5889
FAL: FRAAL2870
ACE: Acel_1186
NML: Namu_2971
GOB: Gobs_2679
BSD: BLASA_2678(dop)
MMAR: MODMU_2599
KRA: Krad_1871
SEN: SACE_2249
SACC: EYD13_12285(dop)
AMD: AMED_4896
AMN: RAM_24920
AMM: AMES_4837
AMZ: B737_4837
AOI: AORI_3819
AJA: AJAP_20065(dop)
PDX: Psed_3306
PSEE: FRP1_12200
PSEH: XF36_10390
PAUT: Pdca_33530
AMI: Amir_2239
SESP: BN6_31810(dop)
KAL: KALB_3046
ACTI: UA75_12955
ACAD: UA74_12875
AHG: AHOG_12160(dop)
ALO: CRK56425
SAQ: Sare_2359
MIL: ML5_5442
ASE: ACPL_5245
ACTN: L083_5072
AFS: AFR_26415
ACTS: ACWT_5115
CAI: Caci_2424
SNA: Snas_4463
AHE: Arch_0904
TPYO: X956_05425
TBW: NCTC13354_00053(dop)
AHW: NCTC11636_00464(dop_1) NCTC11636_02424(dop_2)
BLO: BL1795
BLJ: BLD_1972
BLN: Blon_2127
BLON: BLIJ_2204
BLF: BLIF_1513
BLL: BLJ_1497
BLB: BBMN68_23
BLM: BLLJ_1468
BLG: BIL_01830
BAD: BAD_0547
BADL: BADO_0560
BLA: BLA_1166
BBB: BIF_00519
BBC: BLC1_0611
BLV: BalV_0615
BLW: W7Y_0642
BLS: W91_0663
BANI: Bl12_0595
BANL: BLAC_03210
BANM: EN10_03250
BDE: BDP_0752
BDN: BBDE_0720
BBI: BBIF_0655
BBP: BBPR_0631
BBF: BBB_0615
BBRU: Bbr_1383
BBRE: B12L_1324
BBRV: B689b_1406
BBRJ: B7017_1585
BBRC: B7019_1571
BBRN: B2258_1358
BBRS: BS27_1401
BBRD: BBBR_1439
BAST: BAST_1109
BTP: D805_1151
BCOR: BCOR_0944
BKS: BBKW_0612
BCAT: BBCT_0549
BPSP: AH67_03525
BANG: BBAG_0452
BPSC: BBPC_0600
BSCA: BBSC_0977
PDO: PSDT_1161
TBI: Tbis_1836
AFO: Afer_0908
AYM: YM304_26070(dop)
MIN: Minf_1283
MKC: kam1_1271
NMV: NITMOv2_0801(dop) NITMOv2_3552(dop)
NIO: NITINOP_1617(dop) NITINOP_3240(dop)
NJA: NSJP_0046(dop) NSJP_1982(dop)
LFC: LFE_0657
MOX: DAMO_0750
 » show all
Taxonomy
Reference
1  [PMID:19448618]
  Authors
Striebel F, Imkamp F, Sutter M, Steiner M, Mamedov A, Weber-Ban E
  Title
Bacterial ubiquitin-like modifier Pup is deamidated and conjugated to substrates  by distinct but homologous enzymes.
  Journal
Nat Struct Mol Biol 16:647-51 (2009)
DOI:10.1038/nsmb.1597
  Sequence
[mtu:Rv2112c]
Reference
2  [PMID:20705495]
  Authors
Burns KE, Cerda-Maira FA, Wang T, Li H, Bishai WR, Darwin KH
  Title
"Depupylation" of prokaryotic ubiquitin-like protein from mycobacterial proteasome substrates.
  Journal
Mol Cell 39:821-7 (2010)
DOI:10.1016/j.molcel.2010.07.019
  Sequence
[mtu:Rv2112c]
Reference
3  [PMID:23557784]
  Authors
Striebel F, Imkamp F, Ozcelik D, Weber-Ban E
  Title
Pupylation as a signal for proteasomal degradation in bacteria.
  Journal
Biochim Biophys Acta 1843:103-13 (2014)
DOI:10.1016/j.bbamcr.2013.03.022
Other DBs
ExplorEnz - The Enzyme Database: 3.5.1.119
IUBMB Enzyme Nomenclature: 3.5.1.119
ExPASy - ENZYME nomenclature database: 3.5.1.119
BRENDA, the Enzyme Database: 3.5.1.119

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