KEGG ENZYME: 3.5.1.12

ENZYME: 3.5.1.12

Entry

EC 3.5.1.12                 Enzyme

Name

biotinidase;
amidohydrolase biotinidase;
biocytinase;
biotin-amide amidohydrolase

Class

Hydrolases;
Acting on carbon-nitrogen bonds, other than peptide bonds;
In linear amides

Sysname

biocytin amidohydrolase

Reaction(IUBMB)

biocytin + H2O = biotin + L-lysine [RN:R01077]

Reaction(KEGG)

R01077

Substrate

biocytin [CPD:C05552];
H2O [CPD:C00001]

Product

biotin [CPD:C00120];
L-lysine [CPD:C00047]

Comment

The enzyme, found in many bacterial species as well as animals, liberates biotin from biocytin and short biotinylated peptides, but not from biotinylated proteins. It also has activity on biotin esters and biotin amides.

History

EC 3.5.1.12 created 1961, modified 2023

Pathway

ec00780

Biotin metabolism

ec01100

Metabolic pathways

Orthology

K01435

biotinidase

Genes

HSA:

686(BTD)

PTR:	470760(BTD)

PPS:	100983934(BTD)

GGO:	101131296(BTD)

PON:	100458389(BTD)

PPYG:

129033967(BTD)

NLE:	100584490(BTD)

HMH:	116462044(BTD)

SSYN:

129491330(BTD)

MCC:	677722(BTD)

MCF:	101926326(BTD)

MTHB:

126946874

MNI:	105490406(BTD)

CSAB:

103241707(BTD)

CATY:

105578326(BTD)

PANU:

101018867(BTD)

TGE:	112617641(BTD)

MLEU:

105538149(BTD)

RRO:	104656449(BTD)

RBB:	108535861(BTD)

TFN:	117084205(BTD)

PTEH:

111539502(BTD)

CANG:

105505755(BTD)

CJC:	100409568(BTD)

SBQ:	101042865(BTD)

CIMI:

108299906(BTD)

ANAN:

105723809 105727099(BTD)

CSYR:

103249273(BTD)

MMUR:

105872706(BTD)

LCAT:

123628581(BTD)

PCOQ:

105826206(BTD)

OGA:	100943917(BTD)

MMU:	26363(Btd)

MCAL:

110309042(Btd)

MPAH:

110325552(Btd)

RNO:	306262(Btd)

MCOC:

116084403(Btd)

ANU:	117706132(Btd)

ASYL:

127690589(Btd)

MUN:	110543129 110551270(Btd)

CGE:	100755324(Btd)

MAUA:

101832998(Btd)

PROB:

127217006(Btd)

PLEU:

114708166(Btd)

MORG:

121455239(Btd)

MFOT:

126499146

AAMP:

119827426(Btd)

NGI:	103747521(Btd)

HGL:	101716226(Btd)

CPOC:

100723421(Btd)

CCAN:

109694811(Btd)

DORD:

105989900(Btd)

DSP:	122107949(Btd)

DMER:

138824649(Btd)

PLOP:

125358627(Btd)

NCAR:

124968278 124968503

MMMA:

107149653(Btd)

ITI:

101961662

OCU:

100341187

OPI:	101524102(BTD)

TUP:	102476912(BTD)

GVR:	103595213(BTD)

CFA:	477059(BTD)

CLUD:

112660942(BTD)

VVP:	112925620(BTD)

VLG:	121478712(BTD)

NPO:	129507343(BTD)

AML:	100483327(BTD)

UMR:	103679387(BTD)

UAH:	113253913(BTD)

UAR:	123788358(BTD)

ELK:

111155099

LLV:

125104704

MPUF:

101679447(BTD)

MNP:	132011773(BTD)

MLK:	131824519(BTD)

NVS:	122908998(BTD)

ORO:	101380238(BTD)

EJU:	114205726(BTD)

ZCA:	113915824(BTD)

MLX:	118003387(BTD)

NSU:	110570752(BTD)

LWW:	102732709(BTD)

FCA:	101094289(BTD)

PYU:	121033648(BTD)

PCOO:

112849642(BTD)

PBG:	122492012(BTD)

PVIV:

125175576(BTD)

LRUF:

124526160

LGF:	123611589(BTD)

AJU:

106971609

PTG:	102952175(BTD)

PPAD:

109258837(BTD)

PUC:

125921334

PLEZ:

122229711(BTD)

HHV:	120238470(BTD)

BTA:	537669(BTD)

BOM:	102282711(BTD)

BIU:	109561800(BTD)

BBIS:

105000736(BTD)

CHX:	102179988(BTD)

OAS:	101113196(BTD)

BTAX:

128046105(BTD)

CSUM:

138078358(BTD)

ODA:	120880376(BTD)

CCAD:

122445508(BTD)

MREE:

136173631(BTD)

MBEZ:

129549480(BTD)

SSC:	100737057(BTD)

CFR:	102506341(BTD)

CBAI:

105077624(BTD)

CDK:	105096612(BTD)

VPC:	102536252(BTD)

BACU:

103004341(BTD)

BMUS:

118893902(BTD)

LVE:	103080922(BTD)

OOR:	101278082(BTD)

LALB:

132520840(BTD)

GMF:	115863479(BTD)

DLE:	111168158(BTD)

PCAD:

102983963(BTD)

PSIU:

116753297(BTD)

PPHC:

136122415(BTD)

NASI:

112401421(BTD)

ECB:	100063800(BTD)

EPZ:	103561484(BTD)

EAI:	106842762(BTD)

MYB:	102258405(BTD)

MYD:	102759604(BTD)

MMYO:

118668202(BTD)

MLF:	102419448(BTD)

MDT:	132215584(BTD)

MYUM:

139022337(BTD)

PKL:	118715367(BTD)

EFUS:

103299853(BTD)

MNA:	107542412(BTD)

DRO:	112309951(BTD)

SHON:

118997593(BTD)

AJM:	119048309(BTD)

PDIC:

114502076(BTD)

PHAS:

123830655(BTD)

MMF:	118633287(BTD)

PPAM:

129088234(BTD)

HAI:	109395825(BTD)

RFQ:	117037188(BTD)

PALE:

102894216(BTD)

PGIG:

120582056(BTD)

PVP:	105288551 111739437(BTD)

RAY:	107508454(BTD)

MJV:	108403192(BTD)

TOD:	119256862(BTD)

SARA:

101555521(BTD)

SFUM:

130037740(BTD)

SETR:

125997967(BTD)

LAV:	100663934(BTD)

TMU:

101354864

ETF:	101648355(BTD)

MDO:	100030538(BTD)

GAS:	123249200(BTD)

SHR:	100922840(BTD)

AFZ:

127539117

PCW:	110209057(BTD)

TVP:	118850760(BTD)

PBRV:

138161656(BTD)

MLAO:

141494505(BTD)

OAA:	100084040(BTD)

TACU:

119922048(BTD)

GGA:	420639(BTD)

PCOC:

116227086(BTD)

MGP:	100540232(BTD)

CJO:	107309167(BTD)

TPAI:

128086730(BTD)

LMUT:

125696481(BTD)

NMEL:

110394953(BTD)

APLA:

101796688(BTD)

ACYG:

106030251(BTD)

CATA:

118252780(BTD)

AFUL:

116486767(BTD)

TGU:	100226458(BTD)

LSR:	110474622(BTD)

SCAN:

103812912(BTD)

PMOA:

120502675(BTD)

OTC:	121339587(BTD)

PRUF:

121352913(BTD)

ATRC:

139591097(BTD)

GFR:	102043957(BTD)

FAB:	101821386(BTD)

OMA:	130249061(BTD)

PHI:	102104565(BTD)

PMAJ:

107199918(BTD)

CCAE:

111923444(BTD)

CCW:	104689076(BTD)

CBRC:

103614134(BTD)

ACOE:

138106517(BTD)

ETL:	114055684(BTD)

ZAB:	102075280(BTD)

ZLE:	135443844(BTD)

ACHL:

103797008(BTD)

SVG:	106864282(BTD)

MMEA:

130573231(BTD)

HRT:	120760665(BTD)

SATI:

136361508(BTD)

CCIC:

134045957(BTD)

FPG:	101914603(BTD)

FCH:	102056176(BTD)

CCRI:

104156679(BTD)

NNT:	104411675(BTD)

SHAB:

115618476(BTD)

ACUN:

113476796(BTD)

TALA:

104359643(BTD)

ACHC:

115339769(BTD)

HALD:

104323793(BTD)

HLE:	104843122(BTD)

AGEN:

126038078

HHAR:

128146930(BTD)

GCL:

127013139

CSTI:

104556663(BTD)

LDI:	104341838(BTD)

MNB:	103777184(BTD)

DPUB:

104307530(BTD)

PPUS:

135187988(BTD)

AVIT:

104273902(BTD)

BRHI:

104493186(BTD)

EGZ:	104126131(BTD)

NNI:	104013909(BTD)

PCRI:

104036901(BTD)

PCAO:

104045793(BTD)

PADL:

103918399(BTD)

AFOR:

103906634(BTD)

FGA:	104072840(BTD)

GSTE:

104254419(BTD)

CBOY:

140648274(BTD)

CLV:	102085390(BTD)

CNIC:

135984838(BTD)

MUI:	104542479(BTD)

PGUU:

104467522(BTD)

PLET:

104623708(BTD)

EHS:	104504847(BTD)

CMAC:

104483065(BTD)

CUCA:

104059167(BTD)

TEO:	104375118(BTD)

BREG:

104641131(BTD)

OHA:	104333320(BTD)

ACAR:

104529906(BTD)

CPEA:

104397555(BTD)

CVF:	104289893(BTD)

RTD:	128905284(BTD)

CRID:

134511339(BTD)

AAM:	106483475(BTD)

AROW:

112977278(BTD)

NPD:	112957226(BTD)

TGT:	104568400(BTD)

DNE:	112985275(BTD)

SCAM:

104145078(BTD)

ASN:

112549578

AMJ:	102566286(BTD)

CPOO:

109310413(BTD)

GGN:	109291601(BTD)

PSS:	102458076(BTD)

CMY:	102946514(BTD)

CCAY:

125631590(BTD)

DCC:	119850380(BTD)

CPIC:

101939105(BTD)

TST:	117872360(BTD)

CABI:

116837034(BTD)

MRV:	120398935(BTD)

ACS:	100556978(btd)

ASAO:

132778720(BTD)

PVT:	110072153(BTD)

SUND:

121934088(BTD)

PBI:	103068246(BTD)

PMUR:

107282909(BTD)

CTIG:

120307312(BTD)

TSR:	106545056(BTD)

PGUT:

117655107(BTD)

APRI:

131196704(BTD)

PTEX:

113441308(BTD)

NSS:	113415959(BTD)

VKO:	123033836(BTD)

PMUA:

114607098(BTD)

PRAF:

128398466(BTD)

ZVI:	118092351(BTD)

HCG:	128331692(BTD)

STOW:

125441284(BTD)

EMC:	129337494(BTD)

XLA:	108718912(btd.L)

XTR:	100489727(btd)

NPR:	108803235(BTD)

RTEM:

120940232(BTD)

ACAT:

141144470(BTD)

BBUF:

121002205(BTD)

BGAR:

122938308(BTD)

DTC:	141053689(BTD)

PWL:	138262233(BTD)

MUO:	115460131(BTD)

GSH:	117354010(BTD)

DRE:	555376(btd)

SRX:	107732456(btd)

SANH:

107694759(btd)

SGH:

107559753

CCAR:

109080426(btd)

CAUA:

113062563 113062564

CGIB:

127935284

PTET:

122335192(btd)

LROH:

127181793(btd)

GRF:	141289155 141289678

OMC:	131525513(btd)

RKG:	130078432(btd)

MAMB:

125275859 125276131

CIDE:

127501589

CERY:

137037232(btd)

MASI:

127420874

TROS:

130567364(btd)

TDW:	130415564(btd)

MANU:

129455939(btd)

PDAB:

135775007(btd)

IPU:	108256913(btd)

IFU:	128600342(btd)

PHYP:

113536296(btd)

SMEO:

124375894(btd)

TFD:	113661654(btd)

TVC:	132863812(btd)

CGAR:

128518651(btd)

NGRA:

132900502(btd)

TRN:	134309164(btd)

AMEX:

103037138(btd)

CMAO:

118806172(btd)

EEE:	113590652(btd)

CHAR:

105903673(btd)

SPIL:

134073023(btd)

TRU:	101069377(btd)

TFS:	130518580(btd)

TNG:	GSTEN00032985G001

LCO:	104939816 109139331 109139332

NCC:	104944686 104944699(btd)

TBEN:

117477819 117478235

CGOB:

115015920

PGEO:

117454682 117455173

GACU:

117562835 117562836

EMAC:

134874759(btd) 134874760

ELY:	117263564 117264124 117264125 117264127 117264131

EFO:	125904495 125905085 125905086 125905087 125905088 125905089 125905092 125905093 125905095

PLEP:

121958319(btd)

SLUC:

116066869 116066871 118493288(btd)

ECRA:

117956338 117956339(btd)

ESP:

116701988

PFLV:

114567763 114567944 114568312

GAT:	120826662(btd)

PPUG:

119218862(btd)

AFB:	129097277(btd)

CLUM:

117738939(btd)

PSWI:

130197291(btd)

CANA:

137784422(btd)

CVE:	137872708(btd)

MSAM:

119906734 119906832 119907789

SCHU:

122864009 122864010

CUD:	121523277 121523334 121523416 121524115 121524298 121524362

SPUL:

138608891 138609022 138609095 138609424

LMIX:

132991586 132991587(btd)

ALAT:

119014975 119014987

ASTR:

137607712 137607713

MZE:	101466121 101475752

ONL:	100694007 102082273(btd) 109194532 109197882 109198139 109198141 109200911 109200913 109200914 109204954

OAU:	116318428 116333647 116333669(btd) 116333671

OLA:

101173953

OML:	112136498 112136509

CSAI:

133461590(btd)

XMA:	102233822(btd)

XCO:	114155352(btd)

XHE:	116731274(btd)

PRET:

103472253 103472511

PFOR:

103134997 103135181

PLAI:

106936832(btd) 106943235

PMEI:

106922082 106922093

GAF:	122843529(btd)

PPRL:

129378484(btd)

CVG:

107085879

CTUL:

119785857(btd)

GMU:	124879401(btd)

NFU:

107383317

KMR:	108240453(btd)

ALIM:

106521953 106530461

NWH:	119425694(btd)

AOCE:

111573358(btd)

EJA:	138215084(btd)

BFRE:

138618755(btd)

MCEP:

125019389(btd)

CSEM:

103394637(btd)

POV:	109642670(btd)

SSEN:

122786435(btd)

SSOE:

131446917(btd)

HHIP:

117770677 117770876 117770877

HSP:	118124941 118124942 118124943

PPLT:

128461561(btd)

SMAU:

118292177(btd)

LCF:	108877741 108877753

SDU:

111223068

SLAL:

111656913

XGL:	120786265(btd)

HCQ:	109527670(btd)

SSCV:

125987128(btd)

SBIA:

133496294(btd)

PEE:	133395457(btd)

PTAO:

133471163(btd)

BPEC:

110175184

ENY:	140361562(btd)

MALB:

109968104

BSPL:

114865773(btd)

SJO:	128374148(btd)

SSCO:

133997078(btd)

GMH:	115535695(btd)

SASA:

106570600 106588721 106588750

STRU:

115173883 115175361

OTW:	112240210(btd) 112264467

OMY:	110489374(btd) 110496519

OGO:	124011966 124012249 124043172

ONE:

115132671

OKI:	109879885 109908579

OKE:	118380688(btd) 127909650

OMM:	135549554 135551939

OCLA:

139392389

SALP:

112074045(btd)

SNH:	120021968 120041045

CCLU:

121572271 121586635

ELS:	105012460(btd)

OEP:	134006689(btd)

SFM:

108933388

PKI:	111846924(btd)

AANG:

118234546 118234547 118234548 118234549 118234550

AROT:

135262182 135262183 135262185 135262186 135262187 135262195

CCOG:

133137267(btd)

LOC:	102695279(btd)

PSPA:

121315128(btd)

ARUT:

117394718(btd)

PSEX:

120515401(btd)

LCM:	102358398 102358664(BTD)

CMK:	103181262(btd)

RTP:	109911552 109933343

CPLA:

122550256(btd)

HOC:	132816079 132816203

LERI:

129710024(btd)

HAKA:

140732167(btd)

PMRN:

116948225(BTD)

LRJ:	133345176(BTD)

SPU:

575770

LPIC:

129269525

APLC:

110985242

AFIL:

140164749

PFLL:

139150993

DGR:

6565785

PTRU:

123518658

MNZ:

135204293

HAZT:

108668034

CSCU:

111631973 111642029

CVS:	136976664 136976665 136976666 136976674 136976714

PTEP:

107449055 107449061 139425738

ABRU:

129962872 129963244 129981396 129981814 129981872 129981983

UDV:	129215989 129224917

SDM:	118201733 118203125

PMAX:

117333923

DPOL:

127831737 127869322 127875148

OSN:

115226121

LJP:

135465456

XEN:

124446359

» show all

Reference

1 [PMID:13211594]

Authors

THOMA RW, PETERSON WH.

Title

The enzymatic degradation of soluble bound biotin.

Journal

J Biol Chem 210:569-79 (1954)

Reference

2 [PMID:14087327]

Authors

KNAPPE J, BRUEMMER W, BIEDERBICK K.

Title

[PURIFICATION AND PROPERTIES OF BIOTINIDASE FROM SWINE KIDNEY AND LACTOBACILLUS CASEI.]

Journal

Biochem Z 338:599-613 (1963)

Reference

3 [PMID:5081874]

Authors

Pispa J, Koivusalo M.

Title

Actinomycin D-sensitive increase in the biotinidase activity in mouse liver and serum after ethionine feeding.

Journal

Acta Chem Scand 26:2133-5 (1972)
DOI:10.3891/acta.chem.scand.26-2133

Other DBs

ExplorEnz - The Enzyme Database:

3.5.1.12

IUBMB Enzyme Nomenclature:

3.5.1.12

ExPASy - ENZYME nomenclature database:

3.5.1.12

BRENDA, the Enzyme Database:

3.5.1.12

CAS:

9025-15-4

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Gene (246)   
   MGENES (73)   
   RefGene (173)   
Protein sequence (1266)   
   UniProt (1196)   
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   RefSeq(pep) (65)   
DNA sequence (73)   
   RefSeq(nuc) (67)   
   GenBank (3)   
   EMBL (3)   
Protein domain (7)   
   InterPro (5)   
   Pfam (1)   
   PROSITE(DOC) (1)   
All databases (1592)   

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