Hydrolases;
Acting on carbon-nitrogen bonds, other than peptide bonds;
In linear amides
BRITE hierarchy

protein N-terminal glutamine amidohydrolase

N-terminal L-glutaminyl-[protein] + H2O = N-terminal L-glutamyl-[protein] + NH3 [RN:R11560]

R11560
Reaction

N-terminal L-glutaminyl-[protein] [CPD:C02986];
H2O [CPD:C00001]

This enzyme participates in the eukaryotic ubiquitin-dependent Arg/N-end rule pathway of protein degradation, promoting the turnover of intracellular proteins that initiate with Met-Gln. Following the acetylation and removal of the initiator methionine, the exposed N-terminal glutamine is deaminated, resulting in its conversion to L-glutamate. The latter serves as a substrate for EC 2.3.2.8, arginyltransferase, making the protein susceptible to arginylation, polyubiquitination and degradation as specified by the N-end rule.

EC 3.5.1.122 created 2016

1  [PMID:19560421]

Wang H, Piatkov KI, Brower CS, Varshavsky A

Glutamine-specific N-terminal amidase, a component of the N-end rule pathway.

Mol Cell 34:686-95 (2009)
DOI:10.1016/j.molcel.2009.04.032