KEGG   ENZYME: 3.5.1.133
Entry
EC 3.5.1.133                Enzyme                                 

Name
Nalpha-acyl-L-glutamine aminoacylase;
agaA (gene name);
axillary malodor releasing enzyme;
AMRE
Class
Hydrolases;
Acting on carbon-nitrogen bonds, other than peptide bonds;
In linear amides
Sysname
Nalpha-acyl-L-glutamine amidohydrolase (carboxylate-forming)
Reaction(IUBMB)
an Nalpha-acyl-L-glutamine + H2O = L-glutamine + a carboxylate [RN:R12478]
Reaction(KEGG)
R12478
Substrate
Nalpha-acyl-L-glutamine;
H2O [CPD:C00001]
Product
L-glutamine [CPD:C00064];
carboxylate [CPD:C00060]
Comment
Requires Zn2+. The enzyme, characterized from the bacterium Corynebacterium sp. Ax20, hydrolyses odorless Nalpha-acyl-L-glutamine conjugates of short- and medium-chain fatty acids, releasing axillary malodor compounds. While the enzyme is highly specific for the L-glutamine moiety, it is quite promiscuous regarding the acyl moiety. The two most common products of the enzyme's activity in axillary secretions are (2E)-3-methylhex-2-enoate and 3-hydroxy-3-methylhexanoate.
History
EC 3.5.1.133 created 2019
Orthology
K23726  Nalpha-acyl-L-glutamine aminoacylase
Genes
CUV: CUREI_02740
CFK: CFRA_00225
CGV: CGLAU_03140(yxeP2)
CRL: NCTC7448_00499(yxeP_2)
Reference
1  [PMID:12468539]
  Authors
Natsch A, Gfeller H, Gygax P, Schmid J, Acuna G
  Title
A specific bacterial aminoacylase cleaves odorant precursors secreted in the human axilla.
  Journal
J Biol Chem 278:5718-27 (2003)
DOI:10.1074/jbc.M210142200
  Sequence
Reference
2  [PMID:18492161]
  Authors
Natsch A, Gfeller H, Gygax P, Schmid J
  Title
Isolation of a bacterial enzyme releasing axillary malodor and its use as a screening target for novel deodorant formulations.
  Journal
Int J Cosmet Sci 27:115-22 (2005)
DOI:10.1111/j.1467-2494.2004.00255.x
Reference
3  [PMID:17193210]
  Authors
Natsch A, Derrer S, Flachsmann F, Schmid J
  Title
A broad diversity of volatile carboxylic acids, released by a bacterial aminoacylase from axilla secretions, as candidate molecules for the determination of human-body odor type.
  Journal
Chem Biodivers 3:1-20 (2006)
DOI:10.1002/cbdv.200690015
Other DBs
ExplorEnz - The Enzyme Database: 3.5.1.133
IUBMB Enzyme Nomenclature: 3.5.1.133
ExPASy - ENZYME nomenclature database: 3.5.1.133
BRENDA, the Enzyme Database: 3.5.1.133

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