KEGG   ENZYME: 3.5.1.135
Entry
EC 3.5.1.135                Enzyme                                 

Name
N4-acetylcytidine amidohydrolase;
yqfB (gene name)
Class
Hydrolases;
Acting on carbon-nitrogen bonds, other than peptide bonds;
In linear amides
Sysname
N4-acetylcytidine amidohydrolase
Reaction(IUBMB)
N4-acetylcytidine + H2O = cytidine + acetate [RN:R12635]
Reaction(KEGG)
R12635
Substrate
N4-acetylcytidine [CPD:C22293];
H2O [CPD:C00001]
Product
cytidine [CPD:C00475];
acetate [CPD:C00033]
Comment
The enzyme from the bacterium Escherichia coli is one of the smallest known monomeric amidohydrolases (103-amino acids). The enzyme is active towards a wide range of N4-acylcytosines/cytidines, but is by far most active against N4-acetylcytidine.
History
EC 3.5.1.135 created 2020
Orthology
K09900  N4-acetylcytidine amidohydrolase
Genes
ECO: b2900(yqfB)
ECJ: JW2868(yqfB)
ECD: ECDH10B_3074(yqfB)
EBW: BWG_2625(yqfB)
ECOK: ECMDS42_2399(yqfB)
ECE: Z4238(yqfB)
ECS: ECs3772
ECF: ECH74115_4192
ETW: ECSP_3868(yqfB)
ELX: CDCO157_3525
EOI: ECO111_3638(yqfB)
EOJ: ECO26_3989(yqfB)
EOH: ECO103_3476(yqfB)
ECOH: ECRM13516_3620(yqfB)
ESL: O3K_04965
ESO: O3O_20685
ESM: O3M_05010
ECK: EC55989_3187(yqfB)
ECG: E2348C_3153(yqfB)
EOK: G2583_3553(yqfB)
ELH: ETEC_3093
ECP: ECP_2894
ENA: ECNA114_2943(ygfB1)
ECOS: EC958_3182(yqfB)
ECY: ECSE_3163
ECR: ECIAI1_3019(yqfB)
ECQ: ECED1_3359(yqfB)
EUM: ECUMN_3242(yqfB)
ECT: ECIAI39_3316(yqfB)
EOC: CE10_3339(yqfB)
EBR: ECB_02732(yqfB)
EBL: ECD_02732(yqfB)
EBE: B21_02695(yqfB)
EBD: ECBD_0837
ECI: UTI89_C3286(yqfB)
ECZ: ECS88_3180(yqfB)
ECC: c3481(yqfB)
ESE: ECSF_2695
EAB: ECABU_c31820(yqfB)
EDJ: ECDH1ME8569_2802(yqfB)
ELW: ECW_m3153(yqfB)
ELL: WFL_15405
ELC: i14_3201(yqfB)
ELD: i02_3201(yqfB)
ELF: LF82_3650(yqfB)
ECOI: ECOPMV1_03172(yqfB)
ECOJ: P423_15920
EFE: EFER_2836(yqfB)
STY: STY3206
STT: t2968
STM: STM3050(yqfB)
SEO: STM14_3683(yqfB)
SEY: SL1344_3026(yqfB)
SEJ: STMUK_3038(yqfB)
SEB: STM474_3197(yqfB)
SENI: CY43_15905
SPT: SPA2918(yqfB)
SEK: SSPA2720
SEI: SPC_3110(yqfB)
SEC: SCH_2991(yqfB)
SHB: SU5_03551
SENS: Q786_14765
SED: SeD_A3387
SEG: SG2945(yqfB)
SEL: SPUL_3050
SET: SEN2893(yqfB)
SENA: AU38_14705
SENO: AU37_14715
SENV: AU39_14710
SENQ: AU40_16550
SENL: IY59_15310
SEEP: I137_14565
SENE: IA1_14705
SBG: SBG_2644
SBZ: A464_3061
SFV: SFV_2948
SFE: SFxv_3165
SFN: SFy_4108
SFS: SFyv_4187
SFT: NCTC1_03175(yqfB)
SSN: SSON_3053(yqfB)
SBO: SBO_3092(yqfB)
SDY: SDY_3181(yqfB)
ESA: ESA_00431
CSK: ES15_0704
CTU: CTU_34480
KPN: KPN_03328(yqfB)
KPU: KP1_4617
KPP: A79E_0777
KPR: KPR_4595
KPJ: N559_0907
KPNU: LI86_04450
KPNK: BN49_0760
KVA: Kvar_0737
KPE: KPK_0770
KOX: KOX_02455
KOE: A225_4929
EAE: EAE_02845
EAR: CCG33420
CRO: ROD_49331
CKO: CKO_04263
CAMA: F384_15975
RTG: NCTC13098_01075(yqfB)
CLAP: NCTC11466_02220(yqfB)
KIE: NCTC12125_04740(yqfB)
KAS: KATP_07940(yqfB)
METY: MRY16398_09180(yqfB)
AHN: NCTC12129_04088(yqfB)
EBF: D782_0812
YPE: YPO1778
YPK: y2529
YPH: YPC_2503
YPA: YPA_1150
YPN: YPN_2345
YPM: YP_1615
YPZ: YPZ3_1941
YPD: YPD4_1575
YPX: YPD8_1979
YPW: CH59_3389
YPJ: CH55_899
YPV: BZ15_1771
YPL: CH46_3345
YPS: YPTB1654
YPO: BZ17_848
YPY: YPK_2444
YPB: YPTS_1778
YPQ: DJ40_569
YPU: BZ21_1004
YPR: BZ20_321
YPC: BZ23_1282
YPF: BZ19_1081
YEN: YE1787
YEY: Y11_06421
YEW: CH47_1185
YET: CH48_4036
YAL: AT01_799
YFR: AW19_1490
YIN: CH53_4344
YKR: CH54_173
YRO: CH64_839
YRU: BD65_552
SMAR: SM39_2279
SPE: Spro_2802
SPLY: Q5A_014680
SMAF: D781_2616
SERF: L085_14720
SFJ: SAMEA4384070_2846(yqfB)
SOF: NCTC11214_03983(yqfB)
PMR: PMI1241
PHAU: PH4a_15400
PSI: S70_20835
PSX: DR96_3066
ETR: ETAE_1832
ETD: ETAF_1654
ETE: ETEE_3877
PSHI: SAMEA2665130_0990(yqfB)
HIN: HI_1394
HIT: NTHI1757
HIQ: CGSHiGG_00745(valS)
HIU: HIB_15660
HIE: R2846_0875(yqfB)
HIZ: R2866_1006(yqfB)
HIA: H733_1058
HDU: HD_0412
HAP: HAPS_1184
HPAZ: K756_00735
HPAS: JL26_11450
HPAK: JT17_09020
PMU: PM1447(tex)
PMP: Pmu_16990
MHQ: D650_6050
MHAT: B824_20110
MHAE: F382_05010
MHAM: J450_04340
MHAO: J451_05250
MHAL: N220_11150
MHAQ: WC39_03070
MHAY: VK67_03070
MVE: X875_4580
APL: APL_1141
APJ: APJL_1161
APA: APP7_1200
AAN: D7S_00565
BTO: WQG_17800
BTRE: F542_4770
BTRH: F543_5450
BTRA: F544_17670
VCH: VC_1576
VCS: MS6_1359
VCI: O3Y_07645
VVU: VV2_0047
VVY: VVA0554
VPA: VPA1414
VAG: N646_3130
VSP: VS_1232
VAN: VAA_03122
VTA: A0462(yqfB)
VFI: VF_1208(yqfB)
PPR: PBPRA2168
SON: SO_1922(yqfB)
SDN: Sden_1651
SFR: Sfri_0075
SAZ: Sama_2692
SBL: Sbal_2524
SLO: Shew_1494
SSE: Ssed_1824
SHL: Shal_1626
SWD: Swoo_3436
SWP: swp_1781
SVO: SVI_1091
SPSW: Sps_02292
GPS: C427_2682(yqfB)
PIN: Ping_0595
AHA: AHA_3170
ASA: ASA_1150
AVR: B565_0990
AMED: B224_1029
ACAV: VI35_05495
AEL: NCTC12917_01151(yqfB)
 » show all
Reference
1  [PMID:15969587]
  Authors
Shen Y, Atreya HS, Liu G, Szyperski T
  Title
G-matrix Fourier transform NOESY-based protocol for high-quality protein structure determination.
  Journal
J Am Chem Soc 127:9085-99 (2005)
DOI:10.1021/ja0501870
  Sequence
[eco:b2900]
Reference
2  [PMID:31964920]
  Authors
Stanislauskiene R, Laurynenas A, Rutkiene R, Aucynaite A, Tauraite D, Meskiene R, Urbeliene N, Kaupinis A, Valius M, Kaliniene L, Meskys R
  Title
YqfB protein from Escherichia coli: an atypical amidohydrolase active towards N(4)-acylcytosine derivatives.
  Journal
Sci Rep 10:788 (2020)
DOI:10.1038/s41598-020-57664-w
  Sequence
[eco:b2900]
Other DBs
ExplorEnz - The Enzyme Database: 3.5.1.135
IUBMB Enzyme Nomenclature: 3.5.1.135
ExPASy - ENZYME nomenclature database: 3.5.1.135
BRENDA, the Enzyme Database: 3.5.1.135

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