KEGG   ENZYME: 3.5.1.136
Entry
EC 3.5.1.136                Enzyme                                 

Name
N,N'-diacetylchitobiose non-reducing end deacetylase;
diacetylchitobiose deacetylase (ambiguous);
cda (gene name)
Class
Hydrolases;
Acting on carbon-nitrogen bonds, other than peptide bonds;
In linear amides
Sysname
N,N'-diacetylchitobiose non-reducing end acetylhydrolase
Reaction(IUBMB)
N,N'-diacetylchitobiose + H2O = beta-D-glucosaminyl-(1->4)-N-acetyl-D-glucosamine + acetate [RN:R12671]
Reaction(KEGG)
R12671
Substrate
N,N'-diacetylchitobiose [CPD:C01674];
H2O [CPD:C00001]
Product
beta-D-glucosaminyl-(1->4)-N-acetyl-D-glucosamine [CPD:C22320];
acetate [CPD:C00033]
Comment
The enzyme, characterized from the archaeons Thermococcus kodakarensis and Pyrococcus horikoshii, deacetylates the non-reducing residue of N,N'-diacetylchitobiose, the end product of the archaeal chitinase, to produce beta-D-glucosaminyl-(1->4)-N-acetyl-D-glucosamine. This is in contrast to EC 3.5.1.105, chitin disaccharide deacetylase, which deacetylates N,N'-diacetylchitobiose at the reducing residue to produce N-acetyl-beta-D-glucosaminyl-(1->4)-D-glucosamine.
History
EC 3.5.1.136 created 2020
Orthology
K22408  N,N'-diacetylchitobiose non-reducing end deacetylase
Genes
PALB: EJC50_18705
PTRI: KDC22_20265
PDEC: H1Q58_12870
ACAE: HYG86_13000
DPH: EHF33_06985
RML: FF011L_20160
RLC: K227x_28550(galB_1)
RHOZ: GXP67_24680
PFU: PF0354
PFI: PFC_01000
PHO: PH0499(PH0499)
PAB: PAB1341
PYS: Py04_0547
TKO: TK1764
TGA: TGAM_0594(lmbE)
THA: TAM4_462
TNU: BD01_1336
TEU: TEU_03555
TCQ: TIRI35C_1803(dac)
PPAC: PAP_04135
SMR: Smar_0709
TPE: Tpen_1195
PSYT: DSAG12_00045(dac_1) DSAG12_02500(dac_3) DSAG12_03776(dac_4)
 » show all
Reference
1  [PMID:15136574]
  Authors
Tanaka T, Fukui T, Fujiwara S, Atomi H, Imanaka T
  Title
Concerted action of diacetylchitobiose deacetylase and exo-beta-D-glucosaminidase in a novel chitinolytic pathway in the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1.
  Journal
J Biol Chem 279:30021-7 (2004)
DOI:10.1074/jbc.M314187200
  Sequence
[tko:TK1764]
Reference
2  [PMID:22713621]
  Authors
Mine S, Ikegami T, Kawasaki K, Nakamura T, Uegaki K.
  Title
Expression, refolding, and purification of active diacetylchitobiose deacetylase from Pyrococcus horikoshii.
  Journal
Protein Expr Purif 84:265-9 (2012)
DOI:10.1016/j.pep.2012.06.002
  Sequence
[pho:PH0499]
Reference
3  [PMID:27456364]
  Authors
Nakamura T, Yonezawa Y, Tsuchiya Y, Niiyama M, Ida K, Oshima M, Morita J, Uegaki K.
  Title
Substrate recognition of N,N'-diacetylchitobiose deacetylase from Pyrococcus horikoshii.
  Journal
J Struct Biol 195:286-293 (2016)
DOI:10.1016/j.jsb.2016.07.015
  Sequence
[pho:PH0499]
Other DBs
ExplorEnz - The Enzyme Database: 3.5.1.136
IUBMB Enzyme Nomenclature: 3.5.1.136
ExPASy - ENZYME nomenclature database: 3.5.1.136
BRENDA, the Enzyme Database: 3.5.1.136

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