KEGG ENZYME: 3.5.1.46

ENZYME: 3.5.1.46

Entry

EC 3.5.1.46                 Enzyme

Name

6-aminohexanoate-oligomer exohydrolase;
6-aminohexanoate-dimer hydrolase;
nylB (gene name);
6-aminohexanoic acid oligomer hydrolase (ambiguous);
N-(6-aminohexanoyl)-6-aminohexanoate amidohydrolase;
nylon-6 hydrolase (ambiguous)

Class

Hydrolases;
Acting on carbon-nitrogen bonds, other than peptide bonds;
In linear amides

Sysname

N-(6-aminohexanoyl)-6-aminohexanoate exoamidohydrolase

Reaction(IUBMB)

(1) [N-(6-aminohexanoyl)]n + H2O = [N-(6-aminohexanoyl)]n-1 + 6-aminohexanoate [RN:R10975];
(2) N-(6-aminohexanoyl)-6-aminohexanoate + H2O = 2 6-aminohexanoate [RN:R00059]

Reaction(KEGG)

R00059 R10975;
(other) R10979

Substrate

[N-(6-aminohexanoyl)]n [CPD:C20988];
H2O [CPD:C00001];
N-(6-aminohexanoyl)-6-aminohexanoate [CPD:C01255]

Product

[N-(6-aminohexanoyl)]n-1;
6-aminohexanoate [CPD:C02378]

Comment

The enzyme is involved in degradation of nylon-6 oligomers. It degrades linear oligomers of 6-aminohexanoate with a degree of polymerization of 2--20 by exo-type cleavage, removing residues sequentially from the N-terminus. Activity decreases with the increase of the polymerization number of the oligomer. cf. EC 3.5.1.117, 6-aminohexanoate-oligomer endohydrolase and EC 3.5.2.12, 6-aminohexanoate-cyclic-dimer hydrolase.

History

EC 3.5.1.46 created 1983, modified 2014

Pathway

ec00930

Caprolactam degradation

ec01120

Microbial metabolism in diverse environments

Orthology

K01453

6-aminohexanoate-oligomer exohydrolase

Genes

SLON:

LGI35_36615

MNF:	JSY13_10210

AMAU:

DSM26151_03960(nylB')

LEU:	Leucomu_04960

LTR:	EVS81_02965

LLUT:

K1X41_10430

LTN:	KVY00_04045(nylB)

ARR:	ARUE_c40930(nylB)

AAU:	AAur_3953(nylB)

PAGO:

IRJ34_19580

NDK:	I601_2859(nylB')

» show all

Reference

1 [PMID:7262074]

Authors

Kinoshita S, Terada T, Taniguchi T, Takene Y, Masuda S, Matsunaga N, Okada H.

Title

Purification and characterization of 6-aminohexanoic-acid-oligomer hydrolase of Flavobacterium sp. Ki72.

Journal

Eur J Biochem 116:547-51 (1981)
DOI:10.1111/j.1432-1033.1981.tb05371.x

Other DBs

ExplorEnz - The Enzyme Database:

3.5.1.46

IUBMB Enzyme Nomenclature:

3.5.1.46

ExPASy - ENZYME nomenclature database:

3.5.1.46

UM-BBD (Biocatalysis/Biodegradation Database):

3.5.1.46

BRENDA, the Enzyme Database:

3.5.1.46

CAS:	75216-15-8

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