KEGG   ENZYME: 3.5.1.60
Entry
EC 3.5.1.60                 Enzyme                                 

Name
N-(long-chain-acyl)ethanolamine deacylase;
NAAA (gene name);
N-acylethanolamine amidohydrolase;
acylethanolamine amidase
Class
Hydrolases;
Acting on carbon-nitrogen bonds, other than peptide bonds;
In linear amides
Sysname
N-(long-chain-acyl)ethanolamine amidohydrolase
Reaction(IUBMB)
N-(long-chain-acyl)ethanolamine + H2O = a long-chain carboxylate + ethanolamine [RN:R02041]
Reaction(KEGG)
R02041
Substrate
N-(long-chain-acyl)ethanolamine [CPD:C04080];
H2O [CPD:C00001]
Product
long-chain carboxylate [CPD:C00347];
ethanolamine [CPD:C00189]
Comment
This lysosomal enzyme acts best on palmitoyl ethanolamide, with lower activity on other N-(long-chain-acyl)ethanolamines. It is only active at acidic pH. Unlike EC 3.5.1.99, fatty acid amide hydrolase, it does not act on primary amides such as oleamide, and has only a marginal activity with anandamide. The enzyme is translated as an inactive proenzyme, followed by autocatalytic cleavage into two subunits that reassociate to form an active heterodimeric complex.
History
EC 3.5.1.60 created 1989, modified 2019
Orthology
K13720  N-(long-chain-acyl)ethanolamine deacylase
Genes
HSA: 27163(NAAA)
PTR: 739402(NAAA)
PPS: 100995280(NAAA)
GGO: 101153096(NAAA)
PON: 100450814(NAAA)
NLE: 100596863(NAAA)
MCC: 700991(NAAA)
MCF: 101867426(NAAA)
CSAB: 103235819(NAAA)
CATY: 105576862(NAAA)
PANU: 101013921(NAAA)
RRO: 104673158(NAAA)
RBB: 108532495(NAAA)
TFN: 117085466(NAAA)
PTEH: 111549352(NAAA)
CJC: 100399529(NAAA)
SBQ: 101050123(NAAA)
MMU: 67111(Naaa)
MCAL: 110294786(Naaa)
MPAH: 110330802(Naaa)
RNO: 497009(Naaa)
MCOC: 116104491(Naaa)
MUN: 110555429(Naaa)
CGE: 100769622(Naaa)
PLEU: 114701748(Naaa)
NGI: 103742478(Naaa)
HGL: 101720767(Naaa)
CCAN: 109700833(Naaa)
OCU: 100341112(NAAA)
TUP: 102471067(NAAA)
VVP: 112915592(NAAA)
VLG: 121493427(NAAA)
AML: 100465992(NAAA)
UMR: 103665399(NAAA)
UAH: 113263259(NAAA)
ORO: 101380990(NAAA)
ELK: 111149067
MPUF: 106007225(NAAA)
EJU: 114206755(NAAA)
MLX: 118010438(NAAA)
FCA: 101091867(NAAA)
PTG: 102969278(NAAA)
PPAD: 109277513(NAAA)
AJU: 106981990(NAAA)
HHV: 120231097(NAAA)
BTA: 515375(NAAA)
BOM: 102265385(NAAA)
BIU: 109560592(NAAA)
BBUB: 102392013(NAAA)
CHX: 102185352(NAAA)
OAS: 101105252(NAAA)
ODA: 120861456(NAAA)
SSC: 100521735(NAAA)
CFR: 102512331(NAAA)
CBAI: 105080367(NAAA)
CDK: 105091492(NAAA)
BACU: 102999560(NAAA)
LVE: 103074873(NAAA)
OOR: 101290237(NAAA)
DLE: 111187545(NAAA)
PCAD: 102975801(NAAA)
ECB: 100057831(NAAA)
EPZ: 103562223(NAAA)
EAI: 106846597(NAAA)
MYB: 102245046(NAAA)
MYD: 102774621(NAAA)
MMYO: 118670027(NAAA)
MNA: 107532992(NAAA)
HAI: 109371160(NAAA)
DRO: 112321819(NAAA)
SHON: 118990373(NAAA)
AJM: 119052805(NAAA)
MMF: 118633243(NAAA)
PALE: 102881438(NAAA)
PGIG: 120588222(NAAA)
RAY: 107516370(NAAA)
MJV: 108401469(NAAA)
TOD: 119260782(NAAA)
TMU: 101359649
MDO: 100024418 100024438(NAAA)
SHR: 100924429(NAAA)
PCW: 110209778(NAAA) 110209855
OAA: 103170574(NAAA)
GGA: 422641(NAAA)
PCOC: 116231170(NAAA)
MGP: 100543714(NAAA)
CJO: 107313353(NAAA)
NMEL: 110398291(NAAA)
APLA: 101804788(NAAA)
ACYG: 106043962(NAAA)
TGU: 100223326(NAAA)
LSR: 110470147(NAAA)
SCAN: 103823428(NAAA)
PMOA: 120498806(NAAA)
GFR: 102034425(NAAA)
FAB: 101814088(NAAA)
PHI: 102105273(NAAA)
PMAJ: 107202776(NAAA)
CCAE: 111929446(NAAA)
CCW: 104696818(NAAA)
ETL: 114060040(NAAA)
FPG: 101924134(NAAA)
FCH: 102049864(NAAA)
CLV: 102090769(NAAA)
EGZ: 104125164(NAAA)
NNI: 104023372(NAAA)
ACUN: 113479818(NAAA)
PADL: 103916946(NAAA)
AAM: 106492110(NAAA)
ASN: 102376823(NAAA)
AMJ: 106736764(NAAA)
CPOO: 109309479(NAAA)
GGN: 109301276(NAAA)
PSS: 102448607(NAAA)
CMY: 102939313(NAAA)
CPIC: 101932081(NAAA)
TST: 117878469(NAAA)
CABI: 116817702(NAAA)
ACS: 100558214(naaa)
PVT: 110085256(NAAA)
PBI: 103054033(NAAA)
PMUR: 107291178(NAAA)
TSR: 106552632(NAAA)
PGUT: 117673702(NAAA)
PMUA: 114604572(NAAA)
XLA: 379721(naaal.L) 447407(naaa.L)
XTR: 105945457 448239(naaa)
SRX: 107720262(naaa)
SGH: 107587498(naaa)
IPU: 108255927(naaa)
PHYP: 113537102(naaa)
AMEX: 103047558(naaa)
EEE: 113572914(naaa)
TRU: 101063012(naaa)
LCO: 104921975(naaa)
NCC: 104959415(naaa)
CGOB: 115008787(naaa)
ELY: 117257505
PLEP: 121960955
SLUC: 116036368
ECRA: 117942926
PFLV: 114554381(naaa)
GAT: 120835204
MSAM: 119886685
CUD: 121507025
MZE: 101470207(naaa)
ONL: 100691345(naaa)
OAU: 116314867
OLA: 101155680(naaa)
OML: 112145699
CVG: 107082339 107095639(naaa)
NFU: 107385793(naaa)
KMR: 108244379
ALIM: 106527167(naaa)
AOCE: 111569668(naaa)
CSEM: 103386497(naaa)
POV: 109629030(naaa)
LCF: 108885561(naaa)
SDU: 111221749(naaa)
SLAL: 111653197(naaa)
XGL: 120806933
HCQ: 109521580(naaa)
BPEC: 110170244(naaa)
MALB: 109956131(naaa)
OTW: 112232833(naaa)
OMY: 110525438
SALP: 111974533(naaa)
SNH: 120055593
ELS: 105014691(naaa)
SFM: 108921819(naaa)
LOC: 102694787(naaa)
PSPA: 121301472
CMK: 103180903(naaa)
RTP: 109918585
BFO: 118424906
BBEL: 109471119
SPU: 577060
APLC: 110981512
CEL: CELE_Y55D5A.3(Y55D5A.3)
CBR: CBG00476
PCAN: 112561225
CRG: 105345837
MYI: 110457963
PMAX: 117327420
NVE: 5514668
EPA: 110253589
ATEN: 116296810
ADF: 107355234
AMIL: 114977594
PDAM: 113677086
SPIS: 111341997
DGT: 114516173
HMG: 100204264
SMIN: v1.2.000929.t1(symbB.v1.2.000929.t1)
 » show all
Reference
1  [PMID:11463796]
  Authors
Ueda N, Yamanaka K, Yamamoto S
  Title
Purification and characterization of an acid amidase selective for N-palmitoylethanolamine, a putative endogenous anti-inflammatory substance.
  Journal
J Biol Chem 276:35552-7 (2001)
DOI:10.1074/jbc.M106261200
Reference
2  [PMID:10431820]
  Authors
Ueda N, Yamanaka K, Terasawa Y, Yamamoto S
  Title
An acid amidase hydrolyzing anandamide as an endogenous ligand for cannabinoid receptors.
  Journal
FEBS Lett 454:267-70 (1999)
DOI:10.1016/s0014-5793(99)00820-0
Reference
3  [PMID:22040171]
  Authors
West JM, Zvonok N, Whitten KM, Wood JT, Makriyannis A
  Title
Mass spectrometric characterization of human N-acylethanolamine-hydrolyzing acid  amidase.
  Journal
J Proteome Res 11:972-81 (2012)
DOI:10.1021/pr200735a
Reference
4  [PMID:17980170]
  Authors
Zhao LY, Tsuboi K, Okamoto Y, Nagahata S, Ueda N
  Title
Proteolytic activation and glycosylation of N-acylethanolamine-hydrolyzing acid amidase, a lysosomal enzyme involved in the endocannabinoid metabolism.
  Journal
Biochim Biophys Acta 1771:1397-405 (2007)
DOI:10.1016/j.bbalip.2007.10.002
  Sequence
[hsa:27163]
Other DBs
ExplorEnz - The Enzyme Database: 3.5.1.60
IUBMB Enzyme Nomenclature: 3.5.1.60
ExPASy - ENZYME nomenclature database: 3.5.1.60
BRENDA, the Enzyme Database: 3.5.1.60
CAS: 99283-61-1

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