KEGG   ENZYME: 3.5.1.60
Entry
EC 3.5.1.60                 Enzyme                                 

Name
N-(long-chain-acyl)ethanolamine deacylase;
NAAA (gene name);
N-acylethanolamine amidohydrolase;
acylethanolamine amidase
Class
Hydrolases;
Acting on carbon-nitrogen bonds, other than peptide bonds;
In linear amides
Sysname
N-(long-chain-acyl)ethanolamine amidohydrolase
Reaction(IUBMB)
N-(long-chain-acyl)ethanolamine + H2O = a long-chain carboxylate + ethanolamine [RN:R02041]
Reaction(KEGG)
R02041
Substrate
N-(long-chain-acyl)ethanolamine [CPD:C04080];
H2O [CPD:C00001]
Product
long-chain carboxylate [CPD:C00347];
ethanolamine [CPD:C00189]
Comment
This lysosomal enzyme acts best on palmitoyl ethanolamide, with lower activity on other N-(long-chain-acyl)ethanolamines. It is only active at acidic pH. Unlike EC 3.5.1.99, fatty acid amide hydrolase, it does not act on primary amides such as oleamide, and has only a marginal activity with anandamide. The enzyme is translated as an inactive proenzyme, followed by autocatalytic cleavage into two subunits that reassociate to form an active heterodimeric complex.
History
EC 3.5.1.60 created 1989, modified 2019
Reference
1  [PMID:11463796]
  Authors
Ueda N, Yamanaka K, Yamamoto S
  Title
Purification and characterization of an acid amidase selective for N-palmitoylethanolamine, a putative endogenous anti-inflammatory substance.
  Journal
J Biol Chem 276:35552-7 (2001)
DOI:10.1074/jbc.M106261200
Reference
2  [PMID:10431820]
  Authors
Ueda N, Yamanaka K, Terasawa Y, Yamamoto S
  Title
An acid amidase hydrolyzing anandamide as an endogenous ligand for cannabinoid receptors.
  Journal
FEBS Lett 454:267-70 (1999)
DOI:10.1016/s0014-5793(99)00820-0
Reference
3  [PMID:22040171]
  Authors
West JM, Zvonok N, Whitten KM, Wood JT, Makriyannis A
  Title
Mass spectrometric characterization of human N-acylethanolamine-hydrolyzing acid  amidase.
  Journal
J Proteome Res 11:972-81 (2012)
DOI:10.1021/pr200735a
Reference
4  [PMID:17980170]
  Authors
Zhao LY, Tsuboi K, Okamoto Y, Nagahata S, Ueda N
  Title
Proteolytic activation and glycosylation of N-acylethanolamine-hydrolyzing acid amidase, a lysosomal enzyme involved in the endocannabinoid metabolism.
  Journal
Biochim Biophys Acta 1771:1397-405 (2007)
DOI:10.1016/j.bbalip.2007.10.002
Other DBs
ExplorEnz - The Enzyme Database: 3.5.1.60
IUBMB Enzyme Nomenclature: 3.5.1.60
ExPASy - ENZYME nomenclature database: 3.5.1.60
BRENDA, the Enzyme Database: 3.5.1.60
CAS: 99283-61-1

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