KEGG   ENZYME: 3.5.1.66Help
Entry
EC 3.5.1.66                 Enzyme                                 

Name
2-(hydroxymethyl)-3-(acetamidomethylene)succinate hydrolase;
compound B hydrolase;
alpha-hydroxymethyl-alpha'-(N-acetylaminomethylene)succinic acid hydrolase
Class
Hydrolases;
Acting on carbon-nitrogen bonds, other than peptide bonds;
In linear amides
BRITE hierarchy
Sysname
2-(hydroxymethyl)-3-(acetamidomethylene)succinate amidohydrolase (deaminating, decarboxylating)
Reaction(IUBMB)
2-(hydroxymethyl)-3-(acetamidomethylene)succinate + 2 H2O = acetate + 2-(hydroxymethyl)-4-oxobutanoate + NH3 + CO2 [RN:R04397]
Reaction(KEGG)
Substrate
2-(hydroxymethyl)-3-(acetamidomethylene)succinate [CPD:C04690];
H2O [CPD:C00001]
Product
acetate [CPD:C00033];
2-(hydroxymethyl)-4-oxobutanoate [CPD:C04106];
NH3 [CPD:C00014];
CO2 [CPD:C00011]
Comment
Involved in the degradation of pyridoxin by Pseudomonas and Arthrobacter.
History
EC 3.5.1.66 created 1989
Pathway
ec00750  Vitamin B6 metabolism
Reference
1  [PMID:3972793]
  Authors
Huynh MS, Snell EE.
  Title
Enzymes of vitamin B6 degradation. Purification and properties of two N-acetylamidohydrolases.
  Journal
J Biol Chem 260:2379-83 (1985)
Other DBs
ExplorEnz - The Enzyme Database: 3.5.1.66
IUBMB Enzyme Nomenclature: 3.5.1.66
ExPASy - ENZYME nomenclature database: 3.5.1.66
UM-BBD (Biocatalysis/Biodegradation Database): 3.5.1.66
BRENDA, the Enzyme Database: 3.5.1.66
CAS: 95829-26-8

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