KEGG   ENZYME: 3.5.1.77
Entry
EC 3.5.1.77                 Enzyme                                 
Name
N-carbamoyl-D-amino-acid hydrolase;
D-N-carbamoylase;
N-carbamoylase (ambiguous);
N-carbamoyl-D-amino acid hydrolase
Class
Hydrolases;
Acting on carbon-nitrogen bonds, other than peptide bonds;
In linear amides
Sysname
N-carbamoyl-D-amino-acid amidohydrolase
Reaction(IUBMB)
an N-carbamoyl-D-amino acid + H2O = a D-amino acid + NH3 + CO2 [RN:R02190]
Reaction(KEGG)
R02190
Substrate
N-carbamoyl-D-amino acid [CPD:C05226];
H2O [CPD:C00001]
Product
D-amino acid [CPD:C00405];
NH3 [CPD:C00014];
CO2 [CPD:C00011]
Comment
This enzyme, along with EC 3.5.1.87 (N-carbamoyl-L-amino-acid hydrolase), EC 5.1.99.5 (hydantoin racemase) and hydantoinase, forms part of the reaction cascade known as the "hydantoinase process", which allows the total conversion of D,L-5-monosubstituted hydantoins into optically pure D- or L-amino acids [2]. It has strict stereospecificity for N-carbamoyl-D-amino acids and does not act upon the corresponding L-amino acids or on the N-formyl amino acids, N-carbamoyl-sarcosine, -citrulline, -allantoin and -ureidopropanoate, which are substrates for other amidohydrolases.
History
EC 3.5.1.77 created 1999, modified 2008
Orthology
K01459  N-carbamoyl-D-amino-acid hydrolase
Genes
SYMK6K13_14705
GQUAWC35_02065 AWC35_21320
BGJAWC36_06180 AWC36_10120
BRBEH207_07550 EH207_15550
BNGEH206_14055 EH206_22905
BIZHC231_12695
LBQCKQ53_07460 CKQ53_16475
LPOPI6N93_10735 I6N93_14635
PAOPat9b_4333
PPAOK3169_19460
AFYBW247_12330
HAAA5892_06310
MGEOCFI10_17390
RPJN234_35240
CNCCNE_BB1p08270
REUReut_B4535
CCUPBKK81_02680
CUPBKK80_02025
CUUBKK79_10250
CPAUEHF44_27650
COXE0W60_29015 E0W60_32345
CUKKB879_35925
PPKU875_08630 U875_19865
PPNODA70_01355 DA70_12555
PPNMLV28_04990 LV28_18140
PRBX636_00975 X636_14310
PSPUNA29_16365
PAPISG18_12590
PVEUC34_02240
PFGAB870_13445
PFIBPI93_003720
BUOBRPE64_CCDS07700
CABKNK8_66600
BPTBpet2330
BAVBAV0674
BTRMSAMEA390648702057
BBROBAU06_21120
BFZBAU07_05280
BOHAKI39_20360
BGMCAL15_06705
BOJCBF45_15540
BGVCAL12_20635 CAL12_23115
AXYAXYL_00271(dcaS1) AXYL_03527(dcaS2)
AXONH44784_019281
AXNAX27061_0269
AXXERS451415_00269
ADTAPT56_20675
AISBUW96_07545 BUW96_27085
ASWCVS48_25380
ACHBDVB37_19280
ADYHLG70_00820 HLG70_05395 HLG70_20395
ACHOH4P35_01440 H4P35_05255 H4P35_08235 H4P35_18995
AMUIPE062_02275 PE062_05010 PE062_15725 PE062_25405
APESFOC84_11505 FOC84_27825
AAEGRA224_01295 RA224_15550
ASELRAS12_20255
AFAUZ73_18230
AFQAFA_08930
AAQUD3M96_10170
PHNPAEH1_03445
BOZDBV39_09420 DBV39_17550
ODHDHf2319_02080
PACRFXN63_03955 FXN63_08570
KGYEHF36_15560
RFRRfer_0149 Rfer_0157
POLBpro_1497
PNAPnap_4069
AAAAcav_1139
AMONH9L24_16385
CKEB5M06_03605
CAQTKAQ61_08385 KAQ61_13975
CODOLAD35_11860
CTEZCT3_35760
AANTHUK68_01705
CENPM9799_15170
RTARta_13990
HRBHrubri_1289
HFRG5S34_07720
GLCJQN73_16720
XYKGT347_05560 GT347_11180 GT347_14065
XYGR9X41_22385
AZRCJ010_02050
ACOMCEW83_16295
MLNA9174_30240
MAMOA6B35_10445
MESMEJ066_08015
MHUAMCHK_0583
MJREB229_30840
MERDEB233_27045
MOHIHQ72_02920
MESOBSQ44_08220
RPODE0E05_03520
KMNHW532_13320
AVQHRR99_15935
AVVRvVAT039_pl03420
AVIAvi_7123
ROYG3A56_25620
NENNCHU2750_43480
NPMQEO92_30500
BJAbll7207(bll7207) blr2576(blr2576) blr3330(blr3330)
BJUBJ6T_21980 BJ6T_64620
BJPRN69_10750 RN69_31320
BRABRADO1782 BRADO1790 BRADO3018
BBTBBta_2095 BBta_2106 BBta_5123
BRSS23_11230 S23_47040 S23_54090
AOLS58_45840 S58_57530 S58_57650
BRADBF49_1415 BF49_2210 BF49_4325
BROBRAD285_2771 BRAD285_5501 BRAD285_5513
BOTCIT37_19090 CIT37_37125 CIT37_40810
BRQCIT40_07380 CIT40_10890 CIT40_27350
BGQX265_07140 X265_24710 X265_28160
BGZXH91_06600 XH91_22750 XH91_26140
BSYMCIT39_06615 CIT39_09900 CIT39_26325
BBETF8237_06985
BARHWN72_17995 WN72_19615 WN72_19655
BVZBRAD3257_5411 BRAD3257_6466 BRAD3257_8085
BDGLPJ38_19675
BBANJ4G43_009285 J4G43_018970
BCANBcanWSM471_11980 BcanWSM471_15640 BcanWSM471_31470
BBRAQA636_11695 QA636_11700
BCOUIC761_05805 IC761_22955 IC761_26410
BXNI3J27_12915 I3J27_31995
BROSQUH67_13280 QUH67_13365
BRAZLRP30_10815 LRP30_15400 LRP30_15440
RBKE0H22_22820
VGOGJW-30_1_04016
TRBHB776_00680
BVVBHK69_12205 BHK69_25495
BVESQO058_20090 QO058_24750
BOPAXW83_01395 AXW83_10640
BOSBSY19_1752 BSY19_3589
BOIBLM15_13055
BOFFQV39_11400 FQV39_21360
BOWNWE53_00320 NWE53_17080
AZCAZC_2017 AZC_2488 AZC_2732
MAQUMaq22A_c06080
MEEDA075_21910
MTEADK419_15100 DK419_15245
MINDmvi_46910 mvi_55840
DEIC4375_16580
DNPN8A98_21145
PLEOOHA_1_03075
HDIHDIA_3985
MEYTM49_22335
MAADAZF01_19145
MMEDMame_04417
MLUTJET14_20020
BRND1F64_21940
PHRC6569_06625 C6569_08555 C6569_21545
PSTGE8M01_05625 E8M01_16700 E8M01_26435 E8M01_32015
PAQTE8L99_00285 E8L99_09540 E8L99_15630
LIZLGH83_14250
ACUTMRB58_00385
KVLKVU_0825 KVU_PB0054
KVUEIO_1330 EIO_3237
CONTQ29_07940
YPACCEW88_01065 CEW88_23890
YANAYJ57_10415 AYJ57_23835
SULZC1J03_23710
AHTANTHELSMS3_01544(amiF)
SAGUCDO87_02775
THAACFI11_03685 CFI11_15105
LSALKBK07_10415
RSPRSP_3461
RSHRsph17029_3106
RSQRsph17025_2066
RSKRSKD131_3627
CAZTLV780_09225
PYEA6J80_20965
PSAPJHX88_05470
PFISJHX87_03095
RHCRGUI_1328
THWBMG03_04910
TECAKL02_004640
GEHHYN69_10460
PPRUFDP22_04880 FDP22_18770
PSHQF3W81_03065 F3W81_06000
HDHG5B40_02335 G5B40_15630
CMAGCBW24_01705 CBW24_04755
AMAQGO499_03875
NOGGKE62_14875
GALA0U94_09480
GSPIGS75_03230
GXYGLX_01010
ATOCIW82_00100 CIW82_00705
RGIRGI145_06175 RGI145_18315 RGI145_19745
ROSCTJ15_11225
RMUCFOB66_02190 FOB66_07990
RMTIAI58_08255
RFLRmf_30010 Rmf_30050
RCVPFY06_08160 PFY06_13065
NCHA0U93_11475
LCKHN018_03445
KHAIFJ82_08940
SRFLHU95_06960 LHU95_14475
RAPRHOA_3660 RHOA_5130
NGUQN315_12765
SHUMSTHU_24680 STHU_48090
SVCSTVA_30970 STVA_48280
STELSTAQ_22290 STAQ_43420
FERFNB15_10120
HTQFRZ44_28060
HADHFRZ61_19520
ELIOKO353_11735 KO353_11915
NACINUH88_16540 NUH88_17850 NUH88_20375
THART8K17_13570 T8K17_21805
GSDM3M28_11550
ACRYAC20117_08900
SERWFY030_06590
BLUTEW640_08835
AYMYM304_01590
IAMHC251_12255
IMAPO878_02825
GAZPan241w_18590
GAWV144x_40270
 » show all
Reference
1  [PMID:8462543]
  Authors
Ogawa J, Shimizu S, Yamada H.
  Title
N-carbamoyl-D-amino acid amidohydrolase from Comamonas sp. E222c purification and characterization.
  Journal
Eur J Biochem 212:685-91 (1993)
DOI:10.1111/j.1432-1033.1993.tb17706.x
Reference
2  [PMID:11849938]
  Authors
Altenbuchner J, Siemann-Herzberg M, Syldatk C
  Title
Hydantoinases and related enzymes as biocatalysts for the synthesis of unnatural chiral amino acids.
  Journal
Curr Opin Biotechnol 12:559-63 (2001)
DOI:10.1016/S0958-1669(01)00263-4
Other DBs
ExplorEnz - The Enzyme Database: 3.5.1.77
IUBMB Enzyme Nomenclature: 3.5.1.77
ExPASy - ENZYME nomenclature database: 3.5.1.77
BRENDA, the Enzyme Database: 3.5.1.77
CAS: 71768-08-6

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