KEGG   ENZYME: 3.5.1.78
Entry
EC 3.5.1.78                 Enzyme                                 
Name
glutathionylspermidine amidase;
glutathionylspermidine amidohydrolase (spermidine-forming)
Class
Hydrolases;
Acting on carbon-nitrogen bonds, other than peptide bonds;
In linear amides
Sysname
gamma-L-glutamyl-L-cysteinyl-glycine:spermidine amidase
Reaction(IUBMB)
glutathionylspermidine + H2O = glutathione + spermidine [RN:R01918]
Reaction(KEGG)
R01918
Substrate
glutathionylspermidine [CPD:C05730];
H2O [CPD:C00001]
Product
glutathione [CPD:C00051];
spermidine [CPD:C00315]
Comment
Spermidine is numbered so that atom N-1 is in the amino group of the aminopropyl part of the molecule. The enzyme from Escherichia coli is bifunctional and also catalyses the glutathionylspermidine synthase (EC 6.3.1.8) reaction, resulting in a net hydrolysis of ATP.
History
EC 3.5.1.78 created 1999
Pathway
ec00480  Glutathione metabolism
ec01100  Metabolic pathways
Orthology
K01460  glutathionylspermidine amidase/synthetase
Genes
ECO: b2988(gss)
ECJ: JW2956(gss)
ECD: ECDH10B_3165(gss)
EBW: BWG_2706(gsp)
ECOK: ECMDS42_2466(gss)
ECOC: C3026_16345
ECE: Z4342(gsp)
ECS: ECs_3873(gss)
ECF: ECH74115_4300(gsp)
ETW: ECSP_3967(gsp)
ELX: CDCO157_3616
EOI: ECO111_3818(gss)
EOJ: ECO26_4096(gss)
EOH: ECO103_3672(gss)
ECOO: ECRM13514_3891(gsp)
ECOH: ECRM13516_3759(gsp)
ESL: O3K_03980
ESO: O3O_21670
ESM: O3M_04015
ECK: EC55989_3404(gss)
ECG: E2348C_3275(gsp)
EOK: G2583_3714(gsp)
ELR: ECO55CA74_17695
ELH: ETEC_3262
ECW: EcE24377A_3455(gsp)
EUN: UMNK88_3745
ECP: ECP_3073
ENA: ECNA114_3071(gsp)
ECOS: EC958_3382
ECV: APECO1_3433(gsp)
ECOA: APECO78_18730
ECX: EcHS_A3168(gsp)
ECM: EcSMS35_3274(gsp)
ECY: ECSE_3273
ECR: ECIAI1_3137(gss)
ECQ: ECED1_3638(gss)
EUM: ECUMN_3472(gss)
ECT: ECIAI39_3484(gss)
EOC: CE10_3521(gss)
EBR: ECB_02864(gss)
ECOB: C3029_18940
EBL: ECD_02864(gss)
EBE: B21_02813(gsp)
EBD: ECBD_0750
ECI: UTI89_C3410(gsp)
EIH: ECOK1_3407(gsp)
ECZ: ECS88_3370(gss)
ECC: c3725(gsp)
ELO: EC042_3278(gsp)
ELN: NRG857_14830
ESE: ECSF_2819
ECL: EcolC_0705
EKO: EKO11_0725
EKF: KO11_07735(gsp)
EAB: ECABU_c33940(gss)
EDH: EcDH1_0708
EDJ: ECDH1ME8569_2888(gsp)
ELU: UM146_01415
ELW: ECW_m3262(gsp)
ELL: WFL_15935(gsp)
ELC: i14_3416(gsp)
ELD: i02_3416(gsp)
ELP: P12B_c3091
ELF: LF82_0937(gsp)
ECOL: LY180_15470
ECOI: ECOPMV1_03293(gsp_1)
ECOJ: P423_16880
EFE: EFER_2930(gss)
EAL: EAKF1_ch2960(gsp)
EMA: C1192_20565
ESZ: FEM44_06000
ERUY: OSH18_16620(gss)
STY: STY3310(gsp)
STT: t3060(gsp)
SEX: STBHUCCB_32280
SENT: TY21A_15460
STM: STM3139(gsp)
SEO: STM14_3802(gsp)
SEV: STMMW_30991
SEY: SL1344_3113(gsp)
SEM: STMDT12_C31940
SEJ: STMUK_3127(gsp)
SEB: STM474_3289(gsp)
SEF: UMN798_3414(gsp)
SETU: STU288_15895
SETC: CFSAN001921_01320
SENR: STMDT2_30331(gsp)
SEND: DT104_31351(gsp)
SENI: CY43_16365
SEEN: SE451236_21815
SPT: SPA3007(gsp)
SEK: SSPA2805
SPQ: SPAB_03921
SEI: SPC_3208(gsp)
SEC: SCH_3080(gsp)
SEH: SeHA_C3386
SHB: SU5_03637
SENH: CFSAN002069_16450
SEEH: SEEH1578_01830
SEE: SNSL254_A3392
SENN: SN31241_42660
SEW: SeSA_A3321
SEA: SeAg_B3312
SENS: Q786_15245
SED: SeD_A3486
SEL: SPUL_3145(gsp)
SEGA: SPUCDC_3131(gsp)
SET: SEN2982(gsp)
SENA: AU38_15165
SENO: AU37_15170
SENV: AU39_15170
SENQ: AU40_17095
SENL: IY59_15770
SENJ: CFSAN001992_17850
SEEC: CFSAN002050_22640
SEEB: SEEB0189_004180
SEEP: I137_15025
SENB: BN855_32150(gsp)
SENE: IA1_15160
SENC: SEET0819_22700
SES: SARI_04484
SBG: SBG_2738(gsp)
SBZ: A464_3170
SBV: N643_13630
SALZ: EOS98_03620
SFL: SF3035(gsp)
SFX: S3236(gsp)
SFV: SFV_3041(gsp)
SFE: SFxv_3331(gsp)
SFN: SFy_4331
SFS: SFyv_4411
SFT: NCTC1_03289(gsp_1)
SSN: SSON_3133(gsp)
SBO: SBO_2875(gsp)
SBC: SbBS512_E3415(gsp)
SDY: SDY_3085(gsp)
SDZ: Asd1617_04123
SHQ: A0259_18680
ENC: ECL_04317
ENL: A3UG_19120
ECLG: EC036_37110
ECLE: ECNIH2_19370
ECLN: ECNIH4_03990
ECLI: ECNIH5_18175
ECLX: LI66_18940
ECLY: LI62_20885
ECLZ: LI64_17800
ECLO: ENC_32350
EHM: AB284_06740
EXF: BFV63_18605
ECLA: ECNIH3_18260
ECLC: ECR091_18195
EAU: DI57_00510
EKB: BFV64_19475
ENO: ECENHK_18750
EEC: EcWSU1_03782(gsp)
ELG: BH714_20860
ECAN: CWI88_03460
ERN: BFV67_18410
ECLS: LI67_020445
ECHG: FY206_20675
ESH: C1N69_19180
EPT: HWQ17_17130(gss)
ENT: Ent638_3393
EAS: Entas_3655
ENX: NI40_018510
LNI: CWR52_16310
EBG: FAI37_10390
END: A4308_03425
ENZ: G0034_19165(gss)
ENS: HWQ15_04340(gss)
ENK: LOC22_07090(gss)
EHU: D5067_0003750
EMOR: L6Y89_18320(gss)
ENB: ELK40_19195
EQU: OM418_18590(gss)
EPU: QVH39_19475(gss)
EDY: F0320_18070(gss)
KPN: KPN_03416(gss)
KPU: KP1_4691(gss)
KPM: KPHS_45430
KPP: A79E_0706
KPH: KPNIH24_06165
KPZ: KPNIH27_21685
KPV: KPNIH29_22560
KPW: KPNIH30_22880
KPY: KPNIH31_21800
KPG: KPNIH32_23735
KPC: KPNIH10_22635
KPQ: KPR0928_22145
KPT: VK055_4058
KPO: KPN2242_20170
KPJ: N559_0765
KPI: D364_17595
KPA: KPNJ1_00752
KPS: KPNJ2_00792
KPX: PMK1_00892(gsp_1)
KPB: FH42_13350
KPNE: KU54_003700
KPNU: LI86_03705
KPNK: BN49_0688(gsp)
KVA: Kvar_0675
KPE: KPK_0702(gsp)
KPK: A593_07150
KVD: KR75_04635
KVQ: SP68_11160
KQU: AVR78_00945
KQV: B8P98_03730
KOX: KOX_02835
KOE: A225_5016
KOY: J415_06930
KMI: VW41_20185
KOK: KONIH1_25170
KOC: AB185_10955
EAE: EAE_03450
EAR: CCG33297
KLL: BJF97_25060
KLW: DA718_04280
KAR: LGL98_03615(gss)
KGR: JJJ10_03725(gss)
KPAS: LUW96_02790(gss)
KLC: K7H21_03720(gss)
KLM: BWI76_24245
REE: electrica_00668(gsp_2)
KLP: GUC22_03620
ROR: RORB6_22230
RON: TE10_24110
RPLN: B1209_03500
RAO: DSD31_03700
RTG: NCTC13098_00937(gsp_2)
CRO: ROD_35371(gsp)
CKO: CKO_04379
CFD: CFNIH1_02205
CBRA: A6J81_21055
CWE: CO701_01575
CYO: CD187_03895
CPOT: FOB25_15235
CFQ: C2U38_21575
CSED: JY391_18760(gss)
CAMA: F384_25290
CAF: AL524_22950
CIF: AL515_19575
CFAR: CI104_04065
CIR: C2U53_05500
CIE: AN232_08340
CPAR: CUC49_15485
CTEL: GBC03_26840
CITZ: E4Z61_20710
CARS: E1B03_22250
CIX: M4I31_03795(gss)
CIB: HF677_003475(gss)
CENS: P2W74_03590(gss)
CITR: GUC46_03625
EBT: EBL_c18200
CNT: JT31_15700
CEM: LH23_02000
CEN: LH86_01980
PGE: LG71_07735
ESC: Entcl_0756
KLE: AO703_17795
KGO: CEW81_03845
KIE: NCTC12125_04615(gsp_2)
KAS: KATP_07050
KLU: K7B04_08815(gss)
KCY: RIN60_03555(gss)
LPNU: KQ929_03030(gss)
LAZ: A8A57_17815
LEF: LJPFL01_3492
LEW: DAI21_08385
BUF: D8682_25560
BFT: MNO13_14590(gss)
SYM: K6K13_18935(gss)
YRE: HEC60_14520(gss)
IZH: FEM41_08605
PSGC: G163CM_43150(gss)
SUPE: P0H77_18875(gss)
TOE: QMG90_03345(gss)
MPHG: MPHASIOC01_001671(gss)
EBF: D782_0692
EBU: CUC76_19205
EBB: F652_2843
GQU: AWC35_03140
BGJ: AWC36_13915
BIZ: HC231_18740(gss)
TCI: A7K98_07995
TPTY: NCTC11468_01666(gsp_2)
PMR: PMI0183(gsp)
PMIB: BB2000_0343(gsp)
PVL: AOB99_03715
PVG: CRN77_00315
PHAU: PH4a_10915
PROT: BTA34_02605
PCOL: F1325_03865
PCIB: F9282_03685
PPEE: I6G31_05610(gss)
PAPP: QQS39_04090(gss)
PALA: CO695_14560
PRJ: NCTC6933_01941(gsp_1)
PHAG: PZ638_06830(gss)
PZJ: QS795_008450(gss)
MMK: MU9_3160
MWI: MNY66_11630(gss)
EIC: NT01EI_2998
ETD: ETAF_2424
ETE: ETEE_0790(gsp)
ETR: ETAE_2690
ETC: ETAC_12900
EDW: QY76_07070
EDL: AAZ33_14195(gss)
EHO: A9798_03845
HAV: AT03_19415
HPAR: AL518_07170
OPO: DSM2777_05980
PFQ: QQ39_10560
LRI: NCTC12151_03225(gsp_2)
LGB: V8L73_17095(gss)
HPR: PARA_09880
HAY: C3V42_05715
HPIT: NCTC13334_00926(gsp_2)
HHZ: NCTC10839_00106(gsp_1)
HPAA: E5Q53_02020
HSEM: L3077_03535(gss)
MSU: MS0671(gsp)
BSUN: A4G13_01175
ASU: Asuc_0804
AAP: NT05HA_2010
AAZ: ADJ80_02690
AAT: D11S_0204
AAO: ANH9381_0534
AAN: D7S_01327
AAH: CF65_00544
AACN: AANUM_0246
AACT: ACT75_06460
ASEG: NCTC10977_00377(gsp_2)
AGJ: J5A60_08620(gss)
EMO: DM558_01575
EAZ: JHT90_02580(gss)
ENTO: MTZ49_01140(gss)
KAK: Kalk_19435
SDK: KHX94_15980(gss)
SYK: KDN34_11135(gss)
SAVI: JYB87_06100(gss)
COZ: A3Q34_03840
PHA: PSHAa2455(gssA)
PTN: PTRA_a2949(gsp)
PIA: PI2015_0588
PBW: D172_014785
PALN: B0W48_04760
PART: PARC_a0816(gsp)
PTU: PTUN_a3222(gsp)
PNG: PNIG_a3134(gsp)
PDV: FFU37_02065
GNI: GNIT_0241(gsp)
GPS: C427_5446(gsp)
PMES: FX988_01210
PIN: Ping_1235
SAGA: M5M_11030
ZAL: AZF00_15680
MMW: Mmwyl1_0216
MPRI: MP3633_0175
MARD: IBG28_01045(gss)
MFOI: JSY38_10155(gss)
MPRF: J8N69_08685(gss)
MRHI: KDW99_00965(gss)
MPON: MACH16_01760
NCU: F0U83_13140
GAP: GAPWK_0217
GAQ: RAM11_00085(gss)
FPP: FPB0191_02064
ORB: IPMB12_02005(gss)
OMO: RHO11_08135(gss)
OWH: RHO14_01745(gss)
OSV: RHO12_07440(gss)
UBR: RHO15_01505(gss)
AZQ: G3580_01035(gss)
PMAS: NCF86_16320(gss)
RRU: Rru_A3723
RRF: F11_19045
 » show all
Reference
1  [PMID:7775463]
  Authors
Bollinger JM Jr, Kwon DS, Huisman GW, Kolter R, Walsh CT
  Title
Glutathionylspermidine metabolism in Escherichia coli. Purification, cloning, overproduction, and characterization of a bifunctional glutathionylspermidine synthetase/amidase.
  Journal
J Biol Chem 270:14031-41 (1995)
DOI:10.1074/jbc.270.23.14031
  Sequence
[eco:b2988]
Other DBs
ExplorEnz - The Enzyme Database: 3.5.1.78
IUBMB Enzyme Nomenclature: 3.5.1.78
ExPASy - ENZYME nomenclature database: 3.5.1.78
BRENDA, the Enzyme Database: 3.5.1.78
CAS: 171040-71-4

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Pathway (4)   
   KEGG PATHWAY (4)   
Chemical substance (4)   
   KEGG COMPOUND (4)   
Chemical reaction (3)   
   KEGG REACTION (1)   
   KEGG RCLASS (2)   
Gene (2747)   
   KEGG ORTHOLOGY (1)   
   KEGG GENES (378)   
   KEGG MGENES (2094)   
   RefGene (274)   
Protein sequence (309)   
   UniProt (288)   
   SWISS-PROT (2)   
   RefSeq(pep) (3)   
   PDBSTR (15)   
   PMD (1)   
DNA sequence (1189)   
   RefSeq(nuc) (5)   
   GenBank (615)   
   EMBL (569)   
3D Structure (6)   
   PDB (6)   
Protein domain (6)   
   InterPro (5)   
   Pfam (1)   
All databases (4268)   

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