KEGG   ENZYME: 3.5.1.79
Entry
EC 3.5.1.79                 Enzyme                                 
Name
phthalyl amidase
Class
Hydrolases;
Acting on carbon-nitrogen bonds, other than peptide bonds;
In linear amides
Sysname
phthalyl-amide amidohydrolase
Reaction(IUBMB)
a phthalylamide + H2O = phthalic acid + a substituted amine [RN:R05167]
Reaction(KEGG)
R05167
Substrate
phthalylamide [CPD:C06374];
H2O [CPD:C00001]
Product
phthalic acid;
substituted amine [CPD:C00706]
Comment
In the entry, "phthalyl" is used to mean "2-carboxybenzoyl". The enzyme from Xanthobacter agilis hydrolyses phthalylated amino acids, peptides, beta-lactams, aromatic and aliphatic amines. The substituent on nitrogen may be an alkyl group, but may also be complex, giving an amino acid or peptide derivative. Substitutions on the phthalyl ring include 6-F, 6-NH2, 3-OH, and a nitrogen in the aromatic ring ortho to the carboxy group attached to the amine. No cofactors are required
History
EC 3.5.1.79 created 1999
Reference
1
  Authors
Briggs BS, Kreuzman AJ, Whitesitt C, Yeh WK, Zmijewski M.
  Title
Discovery, purification, and properties of o-phthalyl amidase from Xanthobacter agilis.
  Journal
J Mol Catal, B Enzym 2:53-69 (1996)
Reference
2
  Authors
Black TD, Briggs BS, Evans R, Muth WL, Vangala S, Zmijewski MJ.
  Title
o-Phthalyl amidase in the synthesis of Loracarbef: process development using this novel biocatalyst.
  Journal
Biotechnol Lett 18:875-880 (1996)
Reference
3
  Authors
Costello C, Kreuzman A, Zmijewski M.
  Title
Selective deprotection of phthalyl protected proteins.
  Journal
Tetrahedron Lett 37:7469-7472 (1996)
Reference
4
  Authors
Briggs BS, Zmijewski MJ.
  Title
Enzyme from microbial source: phthalyl amidase.
  Journal
Chem Abstr 123:25010 (1995)
Other DBs
ExplorEnz - The Enzyme Database: 3.5.1.79
IUBMB Enzyme Nomenclature: 3.5.1.79
ExPASy - ENZYME nomenclature database: 3.5.1.79
BRENDA, the Enzyme Database: 3.5.1.79
CAS: 169150-79-2

  All links  
Protein sequence (34)   
   UniProt (33)   
   SWISS-PROT (1)   
DNA sequence (50)   
   GenBank (25)   
   EMBL (25)   
Protein domain (2)   
   InterPro (2)   
All databases (86)   

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