| Entry |
|
|---|
| Name |
biuret amidohydrolase;
biuH (gene name)
|
|---|
| Class |
Hydrolases;
Acting on carbon-nitrogen bonds, other than peptide bonds;
In linear amides
 |
|---|
| Sysname |
biuret amidohydrolase
|
|---|
| Reaction(IUBMB) |
biuret + H2O = urea-1-carboxylate + NH3 [RN: R05563]
|
|---|
| Reaction(KEGG) |
|
|---|
| Substrate |
|
|---|
| Product |
|
|---|
| Comment |
The enzyme, characterized from the bacterium Rhizobium leguminosarum bv. viciae 3841, participates in the degradation of cyanuric acid, an intermediate in the degradation of s-triazide herbicides such as atrazine [2-chloro-4-(ethylamino)-6-(isopropylamino)-1,3,5-triazine]. The substrate, biuret, forms by the spontaneous decarboxylation of 1-carboxybiuret in the absence of EC 3.5.1.131, 1-carboxybiuret hydrolase.
|
|---|
| History |
EC 3.5.1.84 created 2000, modified 2008, modified 2019
|
|---|
| Pathway |
| ec01120 | Microbial metabolism in diverse environments |
|
|---|
| Orthology |
|
|---|
| Genes |
» show all
 |
|---|
| Reference |
|
|---|
| Authors |
Cameron SM, Durchschein K, Richman JE, Sadowsky MJ, Wackett LP |
|---|
| Title |
A New Family of Biuret Hydrolases Involved in S-Triazine Ring Metabolism. |
|---|
| Journal |
ACS Catal 2011:1075-1082 (2011) |
|---|
| Sequence |
|
|---|
| Reference |
|
|---|
| Authors |
Esquirol L, Peat TS, Wilding M, Lucent D, French NG, Hartley CJ, Newman J, Scott C |
|---|
| Title |
Structural and biochemical characterization of the biuret hydrolase (BiuH) from the cyanuric acid catabolism pathway of Rhizobium leguminasorum bv. viciae 3841. |
|---|
| Journal |
|
|---|
| Sequence |
|
|---|
| Reference |
|
|---|
| Authors |
Esquirol L, Peat TS, Wilding M, Liu JW, French NG, Hartley CJ, Onagi H, Nebl T, Easton CJ, Newman J, Scott C |
|---|
| Title |
An unexpected vestigial protein complex reveals the evolutionary origins of an s-triazine catabolic enzyme. |
|---|
| Journal |
J Biol Chem 293:7880-7891 (2018) |
|---|
| Other DBs |
| ExplorEnz - The Enzyme Database: | 3.5.1.84 |
| ExPASy - ENZYME nomenclature database: | 3.5.1.84 |
| UM-BBD (Biocatalysis/Biodegradation Database): | 3.5.1.84 |
|
|---|
