Requires Co2+ for maximal activity [1]. N2-Acetylglutamate is not a substrate. This is the final enzyme in the arginine succinyltransferase (AST) pathway for the catabolism of arginine [1]. This pathway converts the carbon skeleton of arginine into glutamate, with the concomitant production of ammonia and conversion of succinyl-CoA into succinate and CoA. The five enzymes involved in this pathway are EC
2.3.1.109 (arginine N-succinyltransferase), EC
3.5.3.23 (N-succinylarginine dihydrolase), EC
2.6.1.11 (acetylornithine transaminase), EC
1.2.1.71 (succinylglutamate-semialdehyde dehydrogenase) and EC
3.5.1.96 (succinylglutamate desuccinylase).