KEGG   ENZYME: 3.5.2.1Help
Entry
EC 3.5.2.1                  Enzyme                                 

Name
barbiturase
Class
Hydrolases;
Acting on carbon-nitrogen bonds, other than peptide bonds;
In cyclic amides
BRITE hierarchy
Sysname
barbiturate amidohydrolase (3-oxo-3-ureidopropanoate-forming)
Reaction(IUBMB)
barbiturate + H2O = 3-oxo-3-ureidopropanoate [RN:R02139]
Reaction(KEGG)
R02139;
(other) R03870 R10777
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Substrate
barbiturate [CPD:C00813];
H2O [CPD:C00001]
Product
3-oxo-3-ureidopropanoate [CPD:C15607]
Comment
Contains zinc and is specific for barbiturate as substrate [3]. Forms part of the oxidative pyrimidine-degrading pathway in some microorganisms, along with EC 1.17.99.4 (uracil/thymine dehydrogenase) and EC 3.5.1.95 (N-malonylurea hydrolase). It was previously thought that the end-products of the reaction were malonate and urea but this has since been disproved [2]. May be involved in the regulation of pyrimidine metabolism, along with EC 2.4.2.9, uracil phosphoribosyltransferase.
History
EC 3.5.2.1 created 1961, modified 2006
Pathway
ec00240  Pyrimidine metabolism
Orthology
K19794  barbiturase
Genes
RHA: RHA1_ro05296
RER: RER_58820(bar)
REY: O5Y_28310
REB: XU06_28640
ROP: ROP_27990(bar)
ROA: Pd630_LPD01826
RFA: A3L23_03491(bar)
RHS: A3Q41_04764(bar)
ICA: Intca_1068
NCA: Noca_1505 Noca_4270
NDK: I601_1320(bar)
PSIM: KR76_07390
SRO: Sros_6541
NML: Namu_5328
GOB: Gobs_0181
MMAR: MODMU_0176(bar)
PSEH: XF36_23335
CAI: Caci_1879
TBI: Tbis_2213
 » show all
Taxonomy
Reference
1  [PMID:12981104]
  Authors
HAYAISHI O, KORNBERG A.
  Title
Metabolism of cytosine, thymine, uracil, and barbituric acid by bacterial enzymes.
  Journal
J Biol Chem 197:717-32 (1952)
Reference
2  [PMID:11485332]
  Authors
Soong CL, Ogawa J, Shimizu S.
  Title
Novel amidohydrolytic reactions in oxidative pyrimidine metabolism: analysis of the barbiturase reaction and discovery of a novel enzyme, ureidomalonase.
  Journal
Biochem Biophys Res Commun 286:222-6 (2001)
DOI:10.1006/bbrc.2001.5356
  Sequence
Reference
3  [PMID:11748240]
  Authors
Soong CL, Ogawa J, Sakuradani E, Shimizu S.
  Title
Barbiturase, a novel zinc-containing amidohydrolase involved in oxidative pyrimidine metabolism.
  Journal
J Biol Chem 277:7051-8 (2002)
DOI:10.1074/jbc.M110784200
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 3.5.2.1
IUBMB Enzyme Nomenclature: 3.5.2.1
ExPASy - ENZYME nomenclature database: 3.5.2.1
BRENDA, the Enzyme Database: 3.5.2.1
CAS: 9025-16-5

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