KEGG ENZYME: 3.5.2.15

ENZYME: 3.5.2.15

Entry

EC 3.5.2.15                 Enzyme

Name

cyanuric acid amidohydrolase;
atzD (gene name);
trzD (gene name)

Class

Hydrolases;
Acting on carbon-nitrogen bonds, other than peptide bonds;
In cyclic amides

Sysname

cyanuric acid amidohydrolase

Reaction(IUBMB)

cyanuric acid + H2O = 1-carboxybiuret [RN:R12332]

Reaction(KEGG)

R12332

Substrate

cyanuric acid [CPD:C06554];
H2O [CPD:C00001]

Product

1-carboxybiuret [CPD:C22084]

Comment

The enzyme catalyses the ring cleavage of cyanuric acid, an intermediate in the degradation of s-triazide herbicides such as atrazine [2-chloro-4-(ethylamino)-6-(isopropylamino)-1,3,5-triazine]. The enzyme is highly specific for cyanuric acid. The product was initially thought to be biuret, but was later shown to be 1-carboxybiuret.

History

EC 3.5.2.15 created 2000, modified 2008, modified 2019

Pathway

ec00791

Atrazine degradation

ec01100

Metabolic pathways

ec01120

Microbial metabolism in diverse environments

Orthology

K03383

cyanuric acid amidohydrolase

Genes

PPSE:

BN5_3198(trzD)

HMI:	soil367_16665

NTG:	NSCAC_1002

AAEO:

BJI67_02625

AFY:	BW247_02915 BW247_09335

ALCA:

ASALC70_01819(trzD_2)

ALCI:

HML84_11465

ADI:

B5T_00742

AXE:

P40_03495

ACII:

C4901_13330

ATY:	A9R16_003775

BGV:	CAL12_05640

VPD:	VAPA_1c50370

VAA:	AX767_09230

CSER:

CCO03_17450

AANT:

HUK68_18800

CENP:

M9799_08035

HYB:

Q5W_10340

ATER:

MW290_21240

RGU:	A4W93_01790

ARUI:

G6M88_15985

AVV:	RvVAT039_39960

AVF:	RvVAR031_42880

AVI:

Avi_5653

RLE:	pRL100353(atzD)

RLG:

Rleg_6452

RLB:	RLEG3_02325

RTR:	RTCIAT899_PC08525

RHL:	LPU83_pLPU83c0721(atzD)

RHR:	CKA34_30155

RGR:	FZ934_06705

RII:	FFM53_035660(atzD)

RRG:	J3P73_33550(atzD)

RLS:	HB780_09660

RLW:	RlegWSM1455_33685(atzD)

RRHO:

PR018_24750

RTU:	PR017_25575

RBW:	RLCC275e_32380(atzD)

NGL:	RG1141_CH21690

NGG:	RG540_CH22560

BJA:	blr7281(atzD)

BJU:	BJ6T_21060

BJP:	RN69_10315

BRA:	BRADO6319(atzD)

BBT:	BBta_1315(atzD)

BRS:	S23_59060(atzD)

AOL:

S58_64660

BRC:	BCCGELA001_07285

BRAD:

BF49_2819

BRO:	BRAD285_6240(atzD)

BRQ:	CIT40_04950

BGQ:	X265_30660

BGZ:	XH91_29120

BSYM:

CIT39_03550

BBET:

F8237_09205

BARH:

WN72_41590

BVZ:	BRAD3257_1357(atzD)

BDG:	LPJ38_11440

BQB:	J4P68_0017310

BBAN:

J4G43_011035

BCAN:

BcanWSM471_31990

BCOU:

IC761_22630 IC761_29235

BXN:	I3J27_06415

BOY:	LQG66_29625

XDI:	EZH22_27145

AZC:	AZC_3203 AZC_3892

AQI:	J5J86_04150

MRD:	Mrad2831_4776

MET:

M446_3816

MOR:

MOC_0480

MAQU:

Maq22A_c23955

MPHY:

MCBMB27_05504

METX:

A3862_29180

MIND:

mvi_43360

MOG:	MMB17_15610

RHZ:	RHPLAN_06430 RHPLAN_06910

PLEO:

OHA_1_04335(trzD)

HDI:	HDIA_3388(trzD)

AALM:

LUX29_03260

PSIN:

CAK95_16640 CAK95_27680

BRN:	D1F64_13985

PHR:	C6569_17010

PSTG:

E8M01_10955

PAQT:

E8L99_04295 E8L99_18415 E8L99_18445

LIZ:	LGH83_07300

ACUT:

MRB58_19190

CID:

P73_3150

KRE:	GWK63_09735

RFL:

Rmf_00570

SRF:	LHU95_13465 LHU95_17030

SKT:	IGS68_15860

SMUC:

JL100_010175

SROE:

JL101_031685

FER:	FNB15_10190 FNB15_10205

AFR:

AFE_0779

AFE:	Lferr_0922

ACU:

Atc_m024

AFI:	Acife_0653

AFJ:	AFERRID_20110

ACIY:

MQE22_10545

NANN:

O0S08_44545

BACF:

AM500_05065

BCO:	Bcell_1233

BLE:	BleG1_0675

SHUA:

PQ477_11855

MLIT:

KDJ21_006895

PJD:	Pjdr2_4927

PBD:	PBOR_34555

PAEN:

P40081_35880

PAEQ:

R50912_32525

PAEA:

R70723_08795

PDH:	B9T62_31045

PTRI:

KDC22_31450

PTJ:	JRJ22_07220

PUI:	PUW25_17735

SAY:

TPY_1352

SAP:	Sulac_2305

CASP:

NQ535_20450

MTA:

Moth_2120

MTHO:

MOTHE_c21730(trzD)

MTHZ:

MOTHA_c22470(trzD)

NWL:	NWFMUON74_55550(atzD)

GOR:

KTR9_4373

GJI:	H1R19_12940

DKN:	NHB83_00420

SPRF:

K4G22_27465

YIA:	LO772_02745

MSF:	IT882_09430

MLIY:

RYJ27_07215

FRI:	FraEuI1c_3137

PDX:

Psed_3188

PSER:

ABRG53_c018

PGX:	OA858_04340

GLP:	Glo7428_2663 Glo7428_2665

» show all

Reference

1 [PMID:1991731]

Authors

Eaton RW, Karns JS

Title

Cloning and comparison of the DNA encoding ammelide aminohydrolase and cyanuric acid amidohydrolase from three s-triazine-degrading bacterial strains.

Journal

J Bacteriol 173:1363-6 (1991)
DOI:10.1128/JB.173.3.1363-1366.1991

Sequence

[ag:AAC61577]

Reference

2 [PMID:1846859]

Authors

Eaton RW, Karns JS

Title

Cloning and analysis of s-triazine catabolic genes from Pseudomonas sp. strain NRRLB-12227.

Journal

J Bacteriol 173:1215-22 (1991)
DOI:10.1128/JB.173.3.1215-1222.1991