KEGG   ENZYME: 3.5.3.22Help
Entry
EC 3.5.3.22                 Enzyme                                 

Name
proclavaminate amidinohydrolase;
PAH;
proclavaminate amidino hydrolase
Class
Hydrolases;
Acting on carbon-nitrogen bonds, other than peptide bonds;
In linear amidines
BRITE hierarchy
Sysname
amidinoproclavaminate amidinohydrolase
Reaction(IUBMB)
amidinoproclavaminate + H2O = proclavaminate + urea [RN:R05357]
Reaction(KEGG)
Substrate
amidinoproclavaminate [CPD:C06657];
H2O [CPD:C00001]
Product
proclavaminate [CPD:C06658];
urea [CPD:C00086]
Comment
Forms part of the pathway for the biosythesis of the beta-lactamase inhibitor clavulanate in Streptomyces clavuligerus. It carries out an intermediary reaction between the first reaction of EC 1.14.11.21, clavaminate synthase, and the second and third reactions of that enzyme. Requires Mn2+.
History
EC 3.5.3.22 created 2003
Pathway
ec00331  Clavulanic acid biosynthesis
ec01130  Biosynthesis of antibiotics
Orthology
K12676  proclavaminate amidinohydrolase
Genes
SFA: Sfla_0557
STRP: F750_6322
SVI: Svir_33400
Taxonomy
Reference
1  [PMID:1998687]
  Authors
Salowe SP, Krol WJ, Iwata-Reuyl D, Townsend CA.
  Title
Elucidation of the order of oxidations and identification of an intermediate in the multistep clavaminate synthase reaction.
  Journal
Biochemistry 30:2281-92 (1991)
Reference
2  [PMID:11472170]
  Authors
Zhou J, Kelly WL, Bachmann BO, Gunsior M, Townsend CA, Solomon EI.
  Title
Spectroscopic studies of substrate interactions with clavaminate synthase 2, a multifunctional alpha-KG-dependent non-heme iron enzyme: correlation with mechanisms and reactivities.
  Journal
J Am Chem Soc 123:7388-98 (2001)
DOI:10.1021/ja004025+
Reference
3  [PMID:12413541]
  Authors
Townsend CA.
  Title
New reactions in clavulanic acid biosynthesis.
  Journal
Curr Opin Chem Biol 6:583-9 (2002)
DOI:10.1016/S1367-5931(02)00392-7
Reference
4  [PMID:7601835]
  Authors
Wu TK, Busby RW, Houston TA, McIlwaine DB, Egan LA, Townsend CA.
  Title
Identification, cloning, sequencing, and overexpression of the gene encoding proclavaminate amidino hydrolase and characterization of protein function in clavulanic acid biosynthesis.
  Journal
J Bacteriol 177:3714-20 (1995)
DOI:10.1128/JB.177.13.3714-3720.1995
Other DBs
ExplorEnz - The Enzyme Database: 3.5.3.22
IUBMB Enzyme Nomenclature: 3.5.3.22
ExPASy - ENZYME nomenclature database: 3.5.3.22
BRENDA, the Enzyme Database: 3.5.3.22

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