KEGG   ENZYME: 3.5.4.42
Entry
EC 3.5.4.42                 Enzyme                                 

Name
N-isopropylammelide isopropylaminohydrolase;
atzC (gene name)
Class
Hydrolases;
Acting on carbon-nitrogen bonds, other than peptide bonds;
In cyclic amidines
Sysname
N-isopropylammelide isopropylaminohydrolase
Reaction(IUBMB)
N-isopropylammelide + H2O = cyanuric acid + isopropylamine [RN:R05560]
Reaction(KEGG)
R05560;
(other) R06967
Substrate
N-isopropylammelide [CPD:C06553];
H2O [CPD:C00001]
Product
cyanuric acid [CPD:C06554];
isopropylamine [CPD:C06748]
Comment
Requires Zn2+. This bacterial enzyme is involved in degradation of the herbicide atrazine. It can hydrolyse other N-substituted amino dihydroxy-s-triazine molecules, and prefers substrates with linear N-alkyl groups to those with branched alkyl groups.
History
EC 3.5.4.42 created 2000 as EC 3.5.99.4, transferred 2016 to EC 3.5.4.42
Pathway
ec00791  Atrazine degradation
ec01100  Metabolic pathways
ec01120  Microbial metabolism in diverse environments
Orthology
K08710  N-isopropylammelide isopropylaminohydrolase
Genes
LTR: EVS81_12860
ARX: ARZXY2_4061(atzC)
AAU: AAur_pTC10212(atzC)
Reference
1  [PMID:9422605]
  Authors
Sadowsky MJ, Tong Z, de Souza M, Wackett LP
  Title
AtzC is a new member of the amidohydrolase protein superfamily and is homologous to other atrazine-metabolizing enzymes.
  Journal
J Bacteriol 180:152-8 (1998)
DOI:10.1128/JB.180.1.152-158.1998
  Sequence
Reference
2  [PMID:12218024]
  Authors
Shapir N, Osborne JP, Johnson G, Sadowsky MJ, Wackett LP
  Title
Purification, substrate range, and metal center of AtzC: the N-isopropylammelide  aminohydrolase involved in bacterial atrazine metabolism.
  Journal
J Bacteriol 184:5376-84 (2002)
DOI:10.1128/JB.184.19.5376-5384.2002
Reference
3  [PMID:26390431]
  Authors
Balotra S, Warden AC, Newman J, Briggs LJ, Scott C, Peat TS
  Title
X-Ray Structure and Mutagenesis Studies of the N-Isopropylammelide Isopropylaminohydrolase, AtzC.
  Journal
PLoS One 10:e0137700 (2015)
DOI:10.1371/journal.pone.0137700
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 3.5.4.42
IUBMB Enzyme Nomenclature: 3.5.4.42
ExPASy - ENZYME nomenclature database: 3.5.4.42
BRENDA, the Enzyme Database: 3.5.4.42
CAS: 203810-02-0

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