KEGG   ENZYME: 3.5.4.43
Entry
EC 3.5.4.43                 Enzyme                                 

Name
hydroxydechloroatrazine ethylaminohydrolase;
atzB (gene name);
2,4-dihydroxy-6-(isopropylamino)-1,3,5-triazine ethylaminohydrolase
Class
Hydrolases;
Acting on carbon-nitrogen bonds, other than peptide bonds;
In cyclic amidines
Sysname
hydroxyatrazine ethylaminohydrolase
Reaction(IUBMB)
hydroxyatrazine + H2O = N-isopropylammelide + ethylamine [RN:R05559]
Reaction(KEGG)
R05559;
(other) R06966
Substrate
hydroxyatrazine [CPD:C06552];
H2O [CPD:C00001]
Product
N-isopropylammelide [CPD:C06553];
ethylamine [CPD:C00797]
Comment
Contains Zn2+. This bacterial enzyme is involved in degradation of the herbicide atrazine. The enzyme has a broad substrate range, and requires a monohydroxylated s-triazine ring with a minimum of one primary or secondary amine substituent and either a chloride or amine leaving group. It catalyses both deamination and dechlorination reactions.
History
EC 3.5.4.43 created 2000 as EC 3.5.99.3, transferred 2016 to EC 3.5.4.43
Pathway
ec00791  Atrazine degradation
ec01100  Metabolic pathways
ec01120  Microbial metabolism in diverse environments
Orthology
K03382  hydroxydechloroatrazine ethylaminohydrolase
Genes
MESM: EJ066_30455
MERD: EB233_28550
EAK: EKH55_4687
RET: RHE_PC00089
REL: REMIM1_PB00101(atzB)
REP: IE4803_PD00305(atzB)
REI: IE4771_PE00321(atzB)
RLE: pRL100336(atzB)
RLT: Rleg2_4464
RLG: Rleg_6466
RHL: LPU83_pLPU83d0291(atzB)
RGA: RGR602_PC00659(atzB)
RHK: Kim5_PD00282(atzB)
MMED: Mame_02713(atzB)
AAU: AAur_pTC10218(atzB)
 » show all
Reference
1  [PMID:9055410]
  Authors
Boundy-Mills KL, de Souza ML, Mandelbaum RT, Wackett LP, Sadowsky MJ
  Title
The atzB gene of Pseudomonas sp. strain ADP encodes the second enzyme of a novel atrazine degradation pathway.
  Journal
Appl Environ Microbiol 63:916-23 (1997)
DOI:10.1128/AEM.63.3.916-923.1997
  Sequence
Reference
2  [PMID:17660279]
  Authors
Seffernick JL, Aleem A, Osborne JP, Johnson G, Sadowsky MJ, Wackett LP
  Title
Hydroxyatrazine N-ethylaminohydrolase (AtzB): an amidohydrolase superfamily enzyme catalyzing deamination and dechlorination.
  Journal
J Bacteriol 189:6989-97 (2007)
DOI:10.1128/JB.00630-07
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 3.5.4.43
IUBMB Enzyme Nomenclature: 3.5.4.43
ExPASy - ENZYME nomenclature database: 3.5.4.43
BRENDA, the Enzyme Database: 3.5.4.43

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