KEGG   ENZYME: 3.5.4.43
Entry
EC 3.5.4.43                 Enzyme                                 
Name
hydroxydechloroatrazine ethylaminohydrolase;
atzB (gene name);
2,4-dihydroxy-6-(isopropylamino)-1,3,5-triazine ethylaminohydrolase
Class
Hydrolases;
Acting on carbon-nitrogen bonds, other than peptide bonds;
In cyclic amidines
Sysname
hydroxyatrazine ethylaminohydrolase
Reaction(IUBMB)
hydroxyatrazine + H2O = N-isopropylammelide + ethylamine [RN:R05559]
Reaction(KEGG)
R05559;
(other) R06966
Substrate
hydroxyatrazine [CPD:C06552];
H2O [CPD:C00001]
Product
N-isopropylammelide [CPD:C06553];
ethylamine [CPD:C00797]
Comment
Contains Zn2+. This bacterial enzyme is involved in degradation of the herbicide atrazine. The enzyme has a broad substrate range, and requires a monohydroxylated s-triazine ring with a minimum of one primary or secondary amine substituent and either a chloride or amine leaving group. It catalyses both deamination and dechlorination reactions.
History
EC 3.5.4.43 created 2000 as EC 3.5.99.3, transferred 2016 to EC 3.5.4.43
Pathway
ec00791  Atrazine degradation
ec01100  Metabolic pathways
ec01120  Microbial metabolism in diverse environments
Orthology
K03382  hydroxydechloroatrazine ethylaminohydrolase
Genes
MESMEJ066_30455
MERDEB233_28550
EAKEKH55_4687
RETRHE_PC00089
RELREMIM1_PB00101(atzB)
REPIE4803_PD00305(atzB)
REIIE4771_PE00321(atzB)
RLEpRL100336(atzB)
RLTRleg2_4464
RLGRleg_6466
RLBRLEG3_02410
RLURLEG12_05310
RTRRTCIAT899_PC01345
RHLLPU83_pLPU83d0291(atzB)
RGARGR602_PC00659(atzB)
RHKKim5_PD00282(atzB)
RHRCKA34_23020
RADCO657_34030
RIIFFM53_035575
RHIDFFM81_022560
RBQJ2J99_24935
RLNJ0663_28690
RBANJ2J98_28180
RRGJ3P73_33465
SZOK8M09_20720
PDESFE840_020510
MOGMMB17_11770
MMEDMame_02713(atzB)
LITFPZ52_15265
PPAFI8N54_15385
PFAAMM59RIKEN_34140
VFAMM35RIKEN_14260
BLIQINP51_10675
LACYA4V08_06920
CSHClosa_1628
CBOLCGC65_10900
DORDesor_4162
DMIDesmer_2264
LTREVS81_12845
ARXARZXY2_4058
AAUAAur_pTC10218(atzB)
ABAOBEQ56_00965
SSMSpirs_1220
OCKEXM22_00140
SBUSpiBuddy_0452
SGPSpiGrapes_0013
SASSMUG09_02485
 » show all
Reference
1  [PMID:9055410]
  Authors
Boundy-Mills KL, de Souza ML, Mandelbaum RT, Wackett LP, Sadowsky MJ
  Title
The atzB gene of Pseudomonas sp. strain ADP encodes the second enzyme of a novel atrazine degradation pathway.
  Journal
Appl Environ Microbiol 63:916-23 (1997)
DOI:10.1128/AEM.63.3.916-923.1997
  Sequence
Reference
2  [PMID:17660279]
  Authors
Seffernick JL, Aleem A, Osborne JP, Johnson G, Sadowsky MJ, Wackett LP
  Title
Hydroxyatrazine N-ethylaminohydrolase (AtzB): an amidohydrolase superfamily enzyme catalyzing deamination and dechlorination.
  Journal
J Bacteriol 189:6989-97 (2007)
DOI:10.1128/JB.00630-07
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 3.5.4.43
IUBMB Enzyme Nomenclature: 3.5.4.43
ExPASy - ENZYME nomenclature database: 3.5.4.43
BRENDA, the Enzyme Database: 3.5.4.43

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