KEGG ENZYME: 3.5.4.44

ENZYME: 3.5.4.44

Entry

EC 3.5.4.44 Enzyme

Name

ectoine hydrolase;
doeA (gene name)

Class

Hydrolases;
Acting on carbon-nitrogen bonds, other than peptide bonds;
In cyclic amidines

Sysname

ectoine aminohydrolase

Reaction(IUBMB)

ectoine + H2O = (2S)-2-acetamido-4-aminobutanoate [RN:R09800]

Reaction(KEGG)

R09800

Substrate

ectoine [CPD:C06231];
H2O [CPD:C00001]

Product

(2S)-2-acetamido-4-aminobutanoate [CPD:C19929]

Comment

The enzyme, found in some halophilic bacteria, is involved in the degradation of the compatible solute ectoine. The enzyme, which belongs to peptidase family M24, only acts in the direction of ectoine hydrolysis. It also produces smaller amounts of (2S)-4-acetamido-2-aminobutanoate, which is recycled back to ectoine by EC 4.2.1.108, ectoine synthase.

History

EC 3.5.4.44 created 2017

Pathway

ec00260

Glycine, serine and threonine metabolism

ec01100

Metabolic pathways

Orthology

K15783

ectoine hydrolase

Genes

CAMA:

F384_12935

IZH:	FEM41_17295(doeA)

SMAF:

D781_2283

SRZ:	AXX16_2864

SERM:

CLM71_11810(doeA)

VAG:

N646_3801

VEX:	VEA_000632

VNA:	PN96_17665

VEJ:	VEJY3_17226

VSC:	VSVS12_01930

VAF:	D1115_22165(doeA)

GHO:	AL542_02290(doeA)

SALY:

E8E00_14710(doeA)

SKS:	FCN78_14010(doeA)

PAP:	PSPA7_4378(eutD)

PMK:

MDS_0056

PCQ:	PcP3B5_00230

PPU:

PPT:

PPI:

PPUH:

PPUD:

PFV:	Psefu_0029

PMON:

X969_02015

PMOT:

X970_02005

PPJ:	RK21_04289

PFE:	PSF113_4816

PMAN:

OU5_4501

PCG:	AXG94_20235

PSTT:

CH92_00105

PBA:	PSEBR_a4653

PBC:	CD58_05095

PDR:	H681_00255

APRS:

BI364_02940

GAI:	IMCC3135_07185(doeA_1) IMCC3135_09905(doeA_2)

CSA:

Csal_2732

HEL:	HELO_3665(doeA)

HCS:	FF32_01830

HAK:	KO116_01203

HAM:

HALO1223

HHU:	AR456_15085

HCO:	LOKO_00473

HSI:	BOX17_04125

HALO:

BWR19_14485

HHH:	CLM76_01780(doeA)

HBE:	BEI_2959(doeA)

HAG:	BB497_00065

HAF:	C8233_14545(doeA)

HALK:

CUU95_10305(doeA)

HVN:	EI420_15845(doeA)

HAA:	A5892_06415

CMAI:

BFX80_03185

KUS:	B9G99_10900

KMA:	B9H00_03900

OCE:	GU3_04560 GU3_08065 GU3_08085

OCM:	CBP12_08025

OPF:	CBP31_10115

ZDF:	AN401_02320 AN401_02345

TSN:	W908_02365

THIN:

CRN91_07760

BVI:	Bcep1808_5462

BCN:

Bcen_5803

BCH:	Bcen2424_6167

BCM:	Bcenmc03_6648

BCJ:

BCAS0027

BCEN:

DM39_6671(eutD)

BCEW:

DM40_5477(eutD)

BCEO:

I35_7026

BAM:

Bamb_6020

BAC:	BamMC406_5776

BMU:

Bmul_6143

BMJ:	BMULJ_05387(pepQ)

BMK:	DM80_6599(eutD)

BMUL:

NP80_5360(eutD)

BCED:

DM42_6398(eutD)

BDL:	AK34_5360(eutD)

BDF:	WI26_29885

BLAT:

WK25_18050

BTEI:

WS51_29690

BPSL:

WS57_18495

BUG:	BC1001_4311

BGF:	BC1003_5085

BYI:	BYI23_B003710

BUO:	BRPE64_BCDS09550

BUE:	BRPE67_BCDS06280

BUQ:	AC233_27920

BXE:

Bxe_C0063

BXB:	DR64_8362(eutD)

BPH:

Bphy_3862

BGE:	BC1002_4838

BPX:	BUPH_00896

BFN:	OI25_3926(eutD)

BCAI:

K788_0000262

PSPW:

BJG93_22495

PARA:

BTO02_06320 BTO02_27255

PTER:

C2L65_27875(doeA)

PGP:	CUJ91_28295(doeA)

PTS:	CUJ90_21845(doeA)

BOH:	AKI39_12960

VEI:

Veis_2149

ACOM:

CEW83_01835(doeA) CEW83_01840(doeA)

ANT:	Arnit_1221

AHS:	AHALO_1645(doeA)

MLO:

mlr7141

MLN:	A9174_29925 A9174_31385 A9174_32045

MCI:	Mesci_2507

MOP:	Mesop_5921

MAM:	Mesau_05393

MAMO:

A6B35_24255 A6B35_31645

MESO:

BSQ44_02090

MESW:

A9K65_013110 A9K65_031170

MESM:

EJ066_28925(doeA)

MESP:

C1M53_04395(doeA)

HOE:	IMCC20628_03272

RPOD:

E0E05_14125(doeA)

SME:

SM_b20434

SMK:	Sinme_3746

SMQ:	SinmeB_4242

SMX:	SM11_pD1188(eutD)

SMI:	BN406_06240

SMEG:

C770_GR4pD1177

SMEL:

SM2011_b20434(eutD)

SMER:

DU99_31140

SMD:

Smed_3693

RHI:	NGR_b23250

SFH:	SFHH103_04917

SFD:	USDA257_c31390

SIX:	BSY16_5544(eutD)

SAME:

SAMCFNEI73_pB0212 SAMCFNEI73_pC1835

SINO:

SS05631_b62080

EAD:	OV14_b1416

EAH:	FA04_31170

ESJ:	SJ05684_b43240

ATU:

Atu4757

ATF:	Ach5_44890(doeA)

ATA:	AWN88_05985

AGR:	AGROH133_09405

ARO:	B0909_16545(doeA)

AGT:	EYD00_19140(doeA)

RET:	RHE_PF00180

REC:	RHECIAT_PC0000374

REL:	REMIM1_PC00118(eutD-1) REMIM1_PC00148(eutD-2) REMIM1_PF00736(eutD-3)

REP:	IE4803_PC00199(eutD)

REI:	IE4771_PD00200(eutD)

RLE:

pRL120054

RLT:	Rleg2_5521

RLG:

Rleg_5242

RLB:	RLEG3_09340

RLU:	RLEG12_04775

RTR:	RTCIAT899_PB01900 RTCIAT899_PC08140(eutD)

RIR:	BN877_II1096

RHL:	LPU83_pLPU83d1324

RGA:	RGR602_PC02175(eutD)

RHN:	AMJ98_PE00274(eutD)

RPHA:

AMC79_PD00407(eutD)

RHT:

NT26_3906

RHX:	AMK02_PD00140(eutD)

RHV:	BA939_21235

RHK:	Kim5_PC00184(eutD)

REZ:	AMJ99_PD00274(eutD)

RJG:	CCGE525_32200(doeA)

NGL:	RG1141_PA13040(eutD)

NGG:	RG540_PA14020(eutD)

NEO:	CYG48_20840(doeA)

SHZ:

shn_23130

OAN:

Oant_3467

OAH:	DR92_3019(eutD)

OPS:	A8A54_16015

OCH:	CES85_4623(eutD)

BIC:	LMTR13_25345 LMTR13_25690 LMTR13_25715 LMTR13_25810 LMTR13_25880 LMTR13_25960

BVV:	BHK69_14645 BHK69_30100 BHK69_30125

BOI:	BLM15_05205

MOC:	BB934_24610

MIV:	C4E04_10105(doeA)

CHEL:

AL346_21850

CDQ:	BOQ54_17555

PHL:

KKY_228

MMYR:

MXMO3_03371

MEY:	TM49_08170

MAAD:

AZF01_00750

MMED:

Mame_00077 Mame_02830(pepQ_3)

BRN:	D1F64_10805(doeA)

HDI:

HDIA_2075

RBM:

TEF_15100

SIL:	SPO1140(doeA)

SIT:	TM1040_1921

RUA:	D1823_06465

RMB:	K529_004490

RSQ:	Rsph17025_3177

JAN:

Jann_0850

RDE:

RD1_3474

RLI:	RLO149_c026710(eutD)

PDE:

Pden_0287

PYE:	A6J80_21895(doeA)

PZH:	CX676_02845(doeA)

PARO:

CUV01_01765(doeA)

PMUT:

DPM13_12380

PARS:

DRW48_07115(doeA)

PGA:	PGA1_c07820

PGL:	PGA2_c07600

PGD:

Gal_02709

PHP:	PhaeoP97_02538

PPIC:

PhaeoP14_00703

PHQ:	D1820_16985

OAT:	OAN307_c10570(eutD)

OAR:	OA238_c05420(eutD1) OA238_c23670(eutD2)

OTM:

OSB_17100

LMD:	METH_14375

LEJ:	ETW24_15270(doeA)

RED:	roselon_00427

PTP:	RCA23_c24340

CID:

P73_1149

CMAG:

CBW24_12515

MALG:

MALG_03274

CON:	TQ29_09645

RSU:	NHU_01997(eutD)

RHM:	B5V46_09455

RHC:

RGUI_3313

HAT:	RC74_00115 RC74_18035

LAP:	ACP90_14950

LAGG:

B0E33_26780

LABR:

CHH27_05510(doeA)

DAA:	AKL17_0186

YAN:	AYJ57_18830

YPAC:

CEW88_15400(doeA)

SUAM:

BOO69_15095

SPSE:

SULPSESMR1_03271(doeA)

SULZ:

C1J03_19360

SULI:

C1J05_07235(doeA)

SULD:

B5M07_16365

DON:	BSK21_06450

TPRO:

Ga0080559_TMP1087 Ga0080559_TMP3721

TOM:	BWR18_17445

PABY:

Ga0080574_TMP3094

THW:	BMG03_16185

RMM:	ROSMUCSMR3_02212(doeA)

ROK:	RAK1035_0974

LVS:	LOKVESSMR4R_00538(doeA)

AHT:	ANTHELSMS3_04452(doeA)

RBG:	BG454_09340

SAGU:

CDO87_04685

THAS:

C6Y53_13270

SALO:

EF888_15660

SEDI:

EBB79_11115 EBB79_14100

HML:	HmaOT1_18505(doeA)

RBZ:	B9057_07075

ATI:	AL072_17685

TXI:

TH3_02590

THAC:

CSC3H3_02845(doeA)

TII:	DY252_18730(doeA)

APM:	HIMB5_00009770

PGV:	SL003B_2647

ABAW:

D5400_05160

MSM:	MSMEG_2494

MSG:	MSMEI_2434

MSB:	LJ00_12415

MSN:	LI99_12415

MSH:	LI98_12420

MGO:	AFA91_27080

SMAL:

SMALA_8299

AGG:	C1N71_08255

LMOI:

VV02_11300

SERJ:

SGUI_0402

ORN:	DV701_10760

» show all

Reference

1 [PMID:20849449]

Authors

Schwibbert K, Marin-Sanguino A, Bagyan I, Heidrich G, Lentzen G, Seitz H, Rampp M, Schuster SC, Klenk HP, Pfeiffer F, Oesterhelt D, Kunte HJ.

Title

A blueprint of ectoine metabolism from the genome of the industrial producer Halomonas elongata DSM 2581(T).

Journal

Environ Microbiol (2010)
DOI:10.1111/j.1462-2920.2010.02336.x

Sequence

[hel:HELO_3665]

Other DBs

ExplorEnz - The Enzyme Database:

3.5.4.44

IUBMB Enzyme Nomenclature:

3.5.4.44

ExPASy - ENZYME nomenclature database:

3.5.4.44

BRENDA, the Enzyme Database:

3.5.4.44

DBGET integrated database retrieval system