KEGG ENZYME: 3.5.4.44

ENZYME: 3.5.4.44

Entry

EC 3.5.4.44                 Enzyme

Name

ectoine hydrolase;
doeA (gene name)

Class

Hydrolases;
Acting on carbon-nitrogen bonds, other than peptide bonds;
In cyclic amidines

Sysname

ectoine aminohydrolase

Reaction(IUBMB)

ectoine + H2O = (2S)-2-acetamido-4-aminobutanoate [RN:R09800]

Reaction(KEGG)

R09800

Substrate

ectoine [CPD:C06231];
H2O [CPD:C00001]

Product

(2S)-2-acetamido-4-aminobutanoate [CPD:C19929]

Comment

The enzyme, found in some halophilic bacteria, is involved in the degradation of the compatible solute ectoine. The enzyme, which belongs to peptidase family M24, only acts in the direction of ectoine hydrolysis. It also produces smaller amounts of (2S)-4-acetamido-2-aminobutanoate, which is recycled back to ectoine by EC 4.2.1.108, ectoine synthase.

History

EC 3.5.4.44 created 2017

Pathway

ec00260

Glycine, serine and threonine metabolism

ec01100

Metabolic pathways

ec01120

Microbial metabolism in diverse environments

Orthology

K15783

ectoine hydrolase

Genes

CAMA:

F384_12935

IZH:	FEM41_17295(doeA)

SMAF:

D781_2283

SRZ:	AXX16_2864

SERM:

CLM71_11810(doeA)

SRHZ:

FO014_21980(doeA)

VAG:

N646_3801

VEX:	VEA_000632

VNA:	PN96_17665

VEJ:	VEJY3_17226

VSC:	VSVS12_01930

VAF:	D1115_22165(doeA)

VNL:	D3H41_20745(doeA)

VKA:	BTD91_20440

GHO:	AL542_02290(doeA)

SALY:

E8E00_14710(doeA)

SKS:	FCN78_14010(doeA)

SCOT:

HBA18_14650(doeA)

SPRO:

N7E60_16580(doeA)

PAP:	PSPA7_4378(eutD)

PMK:

MDS_0056

PFUW:

KF707C_22180

PCQ:	PcP3B5_00230

PMUI:

G4G71_02705(doeA)

PNT:	G5B91_00105(doeA)

PPAA:

B7D75_20030(doeA)

PPU:

PP_4432

PPT:

PPS_0719

PPI:

YSA_00599

PPUH:

B479_04040

PPUD:

DW66_0726 DW66_1185

PFV:	Psefu_0029

PMON:

X969_02015

PMOT:

X970_02005

PPJ:	RK21_04289

PCI:	PCH70_18590

PFE:	PSF113_4816

PMAN:

OU5_4501

PCG:	AXG94_20235

PBA:	PSEBR_a4653

PBC:	CD58_05095

PDR:	H681_00255

PALL:

UYA_00160

PUM:	HGP31_27400(doeA)

PLAL:

FXN65_00110(doeA)

PSEJ:

HNQ25_01235(doeA)

PKH:	JLK41_00140(doeA)

PTY:	JWV26_10520(doeA)

PMAO:

PMYSY11_0800(doeA)

PANH:

HU763_003780(doeA)

PALV:

KSS97_05930(doeA)

PZA:	HU749_024495(doeA)

PTAE:

NCTC10697_00682(pepP_1)

PCAS:

LOY40_04870(doeA)

PASI:

LG197_08815(doeA)

PSTT:

CH92_00105

GAI:	IMCC3135_07185(doeA_1) IMCC3135_09905(doeA_2)

CSA:

Csal_2732

HEL:	HELO_3665(doeA)

HCS:	FF32_01830

HAK:	KO116_01203

HAM:

HALO1223

HHU:	AR456_15085

HCO:	LOKO_00473

HSI:	BOX17_04125

HALO:

BWR19_14485

HHH:	CLM76_01780(doeA)

HBE:	BEI_2959(doeA)

HAG:	BB497_00065

HAF:	C8233_14545(doeA)

HALK:

CUU95_10305(doeA)

HVN:	EI420_15845(doeA)

HOL:	HORIV_52460

HSR:	HSBAA_50420

HMD:	CTT34_14385(doeA)

HAXI:

HAALTHF_21760n

HTT:	HZS52_10955(doeA)

HCAM:

I4484_15860(doeA)

HPIZ:

GYM47_13615(doeA)

HSX:	HNO51_15785(doeA)

HSV:	HNO53_16355(doeA)

HTX:	EKK97_17870(doeA)

HPRO:

LMS44_16955(doeA)

HQD:	K1Y77_13170(doeA)

HBH:	E4T21_15340(doeA)

HAA:	A5892_06415

CMAI:

BFX80_03185

CAMH:

LCW13_14030(doeA)

COBE:

CLAM6_28560

KUS:	B9G99_10900

KMA:	B9H00_03900

KUY:	FY550_04105(doeA)

PAUR:

FGL86_17275(doeA)

NIK:	F5I99_17985

OCE:	GU3_04560 GU3_08065 GU3_08085

OCM:	CBP12_08025

OPF:	CBP31_10115

ZDF:	AN401_02320 AN401_02345

TSN:	W908_02365

THIN:

CRN91_07760

BVI:	Bcep1808_5462

BCM:	Bcenmc03_6648

BCH:	Bcen2424_6167

BCJ:

BCAS0027

BCEN:

DM39_6671(eutD)

BCEW:

DM40_5477(eutD)

BCEO:

I35_7026

BAM:

Bamb_6020

BAC:	BamMC406_5776

BMJ:	BMULJ_05387(pepQ)

BMU:

Bmul_6143

BMK:	DM80_6599(eutD)

BMUL:

NP80_5360(eutD)

BCED:

DM42_6398(eutD)

BDL:	AK34_5360(eutD)

BDF:	WI26_29885

BLAT:

WK25_18050

BTEI:

WS51_29690

BPSL:

WS57_18495

BUG:	BC1001_4311

BGF:	BC1003_5085

BYI:	BYI23_B003710

BUE:	BRPE67_BCDS06280

BUQ:	AC233_27920

BXE:

Bxe_C0063

BXB:	DR64_8362(eutD)

BPH:

Bphy_3862

BGE:	BC1002_4838

BPX:	BUPH_00896

BFN:	OI25_3926(eutD)

BCAI:

K788_0000262

PSPW:

BJG93_22495(doeA)

PARA:

BTO02_06320 BTO02_27255

PTER:

C2L65_27875(doeA)

PGP:	CUJ91_28295(doeA)

PTS:	CUJ90_21845(doeA)

PEW:	KZJ38_29855(doeA)

CABA:

SBC2_38090(doeA)

BUO:	BRPE64_BCDS09550

BOH:	AKI39_12960

PIG:	EGT29_03410

VEI:

Veis_2149

APET:

ToN1_21090(doeA1) ToN1_21100(doeA2)

ACOM:

CEW83_01835(doeA) CEW83_01840(doeA)

MLO:

mlr7141

MLN:	A9174_24825 A9174_29925 A9174_31385 A9174_32045

MCI:	Mesci_2507 Mesci_4400

MCIC:

A4R28_05060 A4R28_21570 A4R28_29720

MOP:	Mesop_4848 Mesop_5921

MAM:	Mesau_05393

MAMO:

A6B35_17580 A6B35_24255

MESW:

A9K65_013110 A9K65_024320 A9K65_031015 A9K65_031170

MESM:

EJ066_12525 EJ066_28925(doeA)

MESP:

C1M53_04395(doeA)

MHUA:

MCHK_0652(doeA) MCHK_1813

MJR:	EB229_24905 EB229_30510(doeA) EB229_32010(doeA) EB229_32765

MERD:

EB233_21625 EB233_26610(doeA) EB233_30455 EB233_30520

MESR:

FGU64_08280(doeA)

MOH:	IHQ72_14425(doeA) IHQ72_31135

MESO:

BSQ44_02090

HOE:	IMCC20628_03272

PHYL:

HB779_21170(doeA)

RPOD:

E0E05_14125(doeA)

NIY:	FQ775_21085(doeA)

NKI:	KW403_16725(doeA)

ORM:	HTY61_13210(doeA)

KMN:	HW532_10920(doeA) HW532_14360

SME:	SM_b20434(eutD)

SMK:	Sinme_3746

SMQ:	SinmeB_4242

SMX:	SM11_pD1188(eutD)

SMI:	BN406_06240

SMEG:

C770_GR4pD1177

SMEL:

SM2011_b20434(eutD)

SMER:

DU99_31140

SMD:

Smed_3693

RHI:	NGR_a01460 NGR_a01470 NGR_b23250

SFH:	SFHH103_04917

SFD:	USDA257_c31390

SIX:	BSY16_5544(eutD)

SAME:

SAMCFNEI73_pB0212 SAMCFNEI73_pB0218 SAMCFNEI73_pC1835

SINO:

SS05631_b62080

EAD:	OV14_b1416

EAH:	FA04_31170

ESJ:	SJ05684_b43240

EAK:	EKH55_4170

EMX:	FKV68_23470 FKV68_31040(doeA) FKV68_31080 FKV68_31715(doeA)

ATU:

Atu4757

ATA:	AWN88_05985

AGR:	AGROH133_09405

ATF:	Ach5_44890(doeA)

ARO:	B0909_16545(doeA)

AGT:	EYD00_19140(doeA)

RET:	RHE_PF00180

REC:	RHECIAT_PC0000374

REL:	REMIM1_PC00109 REMIM1_PC00118(eutD-1) REMIM1_PC00148(eutD-2) REMIM1_PC00149 REMIM1_PE00247 REMIM1_PF00736(eutD-3)

REP:	IE4803_PC00199(eutD)

REI:	IE4771_PB00253 IE4771_PD00200(eutD)

RLE:

pRL120054

RLT:	Rleg2_5521

RLG:

Rleg_5242

RLB:	RLEG3_09340

RLU:	RLEG12_04775

RTR:	RTCIAT899_PB01870 RTCIAT899_PB01900 RTCIAT899_PC08140(eutD)

RIR:	BN877_II1096

RPUS:

CFBP5875_19350(doeA)

RHL:	LPU83_pLPU83d1324

RGA:	RGR602_PC02175(eutD)

RHN:	AMJ98_PE00274(eutD)

RPHA:

AMC79_PD00407(eutD)

RHX:	AMK02_PD00140(eutD)

RHV:	BA939_21235

RHK:	Kim5_PC00184(eutD)

REZ:	AMJ99_PD00274(eutD)

RJG:	CCGE525_32200(doeA)

RHR:	CKA34_29665(doeA)

RAD:	CO657_24830 CO657_26065(doeA)

ROY:	G3A56_16940(doeA)

RHID:

FFM81_023130(doeA)

RBQ:	J2J99_22090(doeA) J2J99_30815 J2J99_30825 J2J99_30865 J2J99_31785 J2J99_31800

RLN:	J0663_22890(doeA)

RBAN:

J2J98_21075(doeA) J2J98_26260 J2J98_26275

RRG:	J3P73_24735(doeA) J3P73_35965

RAW:	NE851_32100(doeA)

RSUL:

N2599_20835(doeA) N2599_21190

RLW:	RlegWSM1455_24195(doeA)

NGL:	RG1141_PA13040(eutD)

NGG:	RG540_PA14020(eutD)

NEO:	CYG48_20840(doeA)

RHT:

NT26_3906

RFV:	RFYW14_03940

SHZ:	shn_23130 shn_29350

SOJ:	K6301_18985(doeA)

ABAW:

D5400_05160

BRJ:	BKD03_11990

OIN:	IAR37_18905(doeA)

BIO:	BR141012304_10758

OAN:

Oant_3467

OAH:	DR92_3019(eutD)

OPS:	A8A54_16015

BPSN:

NIK97_13585(doeA)

OCH:	CES85_4623(eutD)

OCR:	HGK82_19825(doeA)

OCL:	GTN27_18040(doeA)

BRC:	BCCGELA001_29725

BIC:	LMTR13_25345 LMTR13_25690 LMTR13_25715 LMTR13_25880 LMTR13_25960

BARH:

WN72_09825

BVV:	BHK69_14645 BHK69_30100 BHK69_30125

BOI:	BLM15_05205

BOF:	FQV39_24130

LNE:	FZC33_33975(doeA)

APOL:

K9D25_11765(doeA)

MOC:	BB934_24610

MIV:	C4E04_10105(doeA)

CHEL:

AL346_21850

CDQ:	BOQ54_17555

PHL:

KKY_228

PEM:	OF122_17125

DEA:	FPZ08_18115(doeA)

MMYR:

MXMO3_03371

HDI:

HDIA_2075

MEY:	TM49_08170

MAAD:

AZF01_00750

MMED:

Mame_00077 Mame_02830(pepQ_3)

MLUT:

JET14_01955

AALA:

IGS74_01715(doeA) IGS74_03140

AALM:

LUX29_03025(doeA)

BRN:	D1F64_10805(doeA)

NOH:	G5V57_08425 G5V57_30425

RBM:

TEF_15100

LAP:	ACP90_14950

LABR:

CHH27_05510(doeA)

LABP:

FJ695_10260(doeA)

LABT:

FIU93_20645(pepQ2)

LAGG:

B0E33_26780

SIW:	GH266_08155(doeA)

ACUT:

MRB58_12640(doeA)

SIL:	SPO1140(doeA)

SIT:	TM1040_1921

RUA:	D1823_06465

RUT:	FIU92_06570

RCON:

K3740_11545

JAN:

Jann_0850

RDE:

RD1_3474

RLI:	RLO149_c026710(eutD)

RPON:

G3256_07570

PGA:	PGA1_c07820

PGL:	PGA2_c07600

PGD:

Gal_02709

PHP:	PhaeoP97_02538

PPIC:

PhaeoP14_00703

PHQ:	D1820_16985

OAT:	OAN307_c10570(eutD)

OAR:	OA238_c05420(eutD1) OA238_c23670(eutD2)

OTM:

OSB_17100

OCT:	FTO60_07550

LMD:	METH_14375

LEJ:	ETW24_15270(doeA)

LAQU:

R2C4_20340(doeA)

LEV:	ETW23_14595(doeA)

LCAE:

K3721_19445(doeA)

RED:	roselon_00427

CID:

P73_1149

MALG:

MALG_03274

CON:	TQ29_09645

YPAC:

CEW88_15400(doeA)

YAN:	AYJ57_18830

TPRO:

Ga0080559_TMP1087 Ga0080559_TMP3721

PABY:

Ga0080574_TMP3094

SUAM:

BOO69_15095

SULZ:

C1J03_19360

SULI:

C1J05_07235(doeA)

SULD:

B5M07_16365

SPOT:

G6548_05620(doeA)

SMED:

JNX03_02425(doeA)

SINL:

DSM14862_03215(doeA)

SFAV:

PL335_15425(doeA)

SPSE:

SULPSESMR1_03271(doeA)

TOM:	BWR18_17445

RMM:	ROSMUCSMR3_02212(doeA)

ROK:	RAK1035_0974

RID:	RIdsm_05126

ROM:	EI983_17935

ROH:	FIU89_20095

AHT:	ANTHELSMS3_04452(doeA)

SAGU:

CDO87_04685

OCD:	FHY55_15520

MARU:

FIU81_12470

ROT:	FIV09_16800

RMH:	LVO79_14970

MALU:

KU6B_39230

TGL:	HFZ77_11085

HAT:	RC74_00115 RC74_18035

PALX:

GQA70_01790(doeA)

LSAL:

KBK07_07795

RSQ:	Rsph17025_3177

CAZT:

LV780_15620(doeA)

PDE:

Pden_0287

PYE:	A6J80_21895(doeA)

PZH:	CX676_02845(doeA)

PARO:

CUV01_01765(doeA)

PARS:

DRW48_07115(doeA)

PARR:

EOJ32_08975(doeA)

PKD:	F8A10_17635(doeA)

PLIA:

E4191_06420(doeA)

PSAN:

HGN31_00605(doeA)

PMAU:

CP157_01850(doeA)

PMEH:

JWJ88_19995(doeA)

PSEW:

JHW44_09325(doeA)

PSAP:

JHX88_09815(doeA)

PFIS:

JHX87_00890(doeA)

PTP:	RCA23_c24340

RSU:	NHU_01997(eutD)

RHM:	B5V46_09455

RHC:

RGUI_3313

RMB:	K529_004490

DAA:	AKL17_0186

DON:	BSK21_06450

RBZ:	B9057_07075

THW:	BMG03_16185

TEC:	AKL02_015690

LVS:	LOKVESSMR4R_00538(doeA)

RBG:	BG454_09340

SALO:

EF888_15660

SEDI:

EBB79_11115 EBB79_14100

HML:	HmaOT1_18505(doeA)

LIT:	FPZ52_11025(doeA)

PPRU:

FDP22_11890

PAED:

G5B38_12865

MON:	G8E03_07580(doeA)

PSHQ:

F3W81_04280

PALW:

PSAL_009750(doeA)

PPAF:

I8N54_09435

PGV:	SL003B_2647

THAS:

C6Y53_13270

HDH:	G5B40_08315(doeA)

CMAG:

CBW24_12515

GCE:	KYE46_04390

ACRO:

K3J57_13480

SINR:

O5O51_08245(doeA)

AALK:

LGT41_0009040

FAQ:	G5B39_06375(doeA)

AMAQ:

GO499_04025(doeA)

PALG:

HFP57_02005

SHUM:

STHU_07270

ATI:	AL072_17685

HTQ:	FRZ44_00880 FRZ44_08400 FRZ44_35360

HADH:

FRZ61_08310

TXI:

TH3_02590

THAC:

CSC3H3_02845(doeA)

TII:	DY252_18730(doeA)

THAI:

IT893_02690(doeA)

NACI:

NUH88_16395

PUB:	SAR11_1338(y4tM)

PEG:	E5R92_06560

APM:	HIMB5_00009770

SNEA:

NBZ79_05525(doeA)

ANT:	Arnit_1221

AHS:	AHALO_1645(doeA)

AMAR:

AMRN_1667(doeA)

ACAA:

ACAN_1695(doeA)

DMS:	E8L03_14000(doeA)

DWD:	DSCW_13820 DSCW_13910

DALK:

DSCA_33490 DSCA_33560

BFD:	NCTC4823_03387 NCTC4823_03388(pepQ_2) NCTC4823_03395(pepQ_3)

GST:	HW35_17680 HW35_17715

BCO:	Bcell_3360

BMEG:

BG04_5939

PARH:

I5S86_22750(doeA)

BCL:

ABC0020

BLE:	BleG1_4014

BKW:	BkAM31D_11445

BON:	A361_15545

OCN:	CUC15_04445

GTN:	GTNG_2272 GTNG_2288

HHD:	HBHAL_4917

HMN:	HM131_02830

HLI:

HLI_13335

VIR:	X953_14790

VHL:	BME96_14130

VIL:	CFK37_11340

VNE:	CFK40_15750

VIM:	GWK91_09505 GWK91_12205

LAO:	AOX59_17770

LEX:	Len3610_10935

SJE:	AAV35_007875

AQT:	FN924_17770

GRC:	GI584_02830

RUE:	DT065_02165 DT065_13450

SALE:

EPH95_01510 EPH95_13645

MSEM:

GMB29_22080

SEDD:

ERJ70_06570 ERJ70_18310

BBEV:

BBEV_1548(pepP)

STEA:

C0679_03045

SHV:	AAT16_02010

PBJ:	VN24_22255

PRT:	AUC31_05560

PANA:

BBH88_16750

PDG:	BCM40_12125

PHC:	BBI08_16660

PMAR:

B0X71_04260

PPLA:

BBI15_00160

PLX:	CW734_05910

PMAT:

BBI11_05385

PDEC:

H1Q58_04530

PANC:

E2636_15860

KPUL:

GXN76_11640

KEB:	GXN75_13265

CACE:

CACET_c38080

CFM:	BJL90_04765

AMT:

Amet_1194

GFE:	Gferi_03360 Gferi_03415

DMI:	Desmer_3359

FWA:	DCMF_21820

ACAE:

HYG86_07860

HPK:	Hprae_0064

MBAI:

MB901379_01124

MSM:	MSMEG_2494

MSG:	MSMEI_2434

MSB:	LJ00_12415

MSN:	LI99_12415

MSH:	LI98_12420

MGO:	AFA91_27080

CCYC:

SCMU_37550

NNO:	NONO_c26800

RHB:

NY08_428

RFA:	A3L23_03751(doeA)

RHS:	A3Q41_04489(doeA)

GBR:

Gbro_4077

GPO:	GPOL_c44040

GOR:

KTR9_4008

GTA:	BCM27_21060

GRU:	GCWB2_20415

GOM:	D7316_04409(doeA)

GAV:	C5O27_07690

GOD:	GKZ92_19335

GJI:	H1R19_01230 H1R19_19420

GOI:	LK459_13210

GHN:	MVF96_19905

GAMI:

IHQ52_23415

GMG:	NWF22_06865

SMAL:

SMALA_8299

AARC:

G127AT_06580

AGG:	C1N71_08255

PSEV:

USB125703_01939(doeA)

GSD:	M3M28_10520

ACRY:

AC20117_04245

GLU:	F0M17_16010

KPL:	KPaMU14_00695

KFV:	AS188_01275

KRS:	EQG70_03255

KOD:	HBK84_10630

LMOI:

VV02_11300

ARS:	ADJ73_04465

SERJ:

SGUI_0402

ORN:	DV701_10760

ORZ:	FNH13_14110

BLY:	A2T55_10320

BLIN:

BLSMQ_2278

BCAU:

I6G59_13850

GYU:	FE374_14490

PBO:	PACID_31230

AACI:

ASQ49_06565

AJI:	C0Z10_01005

MIK:	FOE78_15800

MICG:

GJV80_09825

NRO:	K8W59_04940

SSM:	Spirs_0573

IPO:	Ilyop_0097

HDL:	HALDL1_11275

HJE:	HacjB3_03825

HGI:	ABY42_16910

HALE:

G3A49_07505

HLA:

Hlac_1051

HALQ:

Hrr1229_000995

SRUB:

C2R22_00565

HALG:

HUG10_11110

HALU:

HUG12_01630

HACB:

Hbl1158_16060

HAH:	Halar_1735

HTU:

Htur_2943

HSAL:

JMJ58_13720

HAKZ:

J0X25_01335

NMG:

Nmag_3760

HXA:	Halxa_0592

NPE:	Natpe_0777

NVR:	FEJ81_04025

NAY:	HYG81_06970

HALY:

HYG82_09925

HJT:	DVR14_13195

HLO:	J0X27_13500

HALV:

NGM15_13095

NOU:	Natoc_1848

HLR:	HALLA_02180

NAG:	AArcMg_2880

NAN:	AArc1_0822

NBG:	DV706_10725

NAS:	GCU68_01335

HDA:	BB347_08890

SAWL:

NGM29_03470

» show all

Reference

1 [PMID:20849449]

Authors

Schwibbert K, Marin-Sanguino A, Bagyan I, Heidrich G, Lentzen G, Seitz H, Rampp M, Schuster SC, Klenk HP, Pfeiffer F, Oesterhelt D, Kunte HJ

Title

A blueprint of ectoine metabolism from the genome of the industrial producer Halomonas elongata DSM 2581 T.

Journal

Environ Microbiol 13:1973-94 (2011)
DOI:10.1111/j.1462-2920.2010.02336.x

Sequence

[hel:HELO_3665]

Other DBs

ExplorEnz - The Enzyme Database:

3.5.4.44

IUBMB Enzyme Nomenclature:

3.5.4.44

ExPASy - ENZYME nomenclature database:

3.5.4.44

BRENDA, the Enzyme Database:

3.5.4.44

DBGET integrated database retrieval system