KEGG   ENZYME: 3.5.4.45
Entry
EC 3.5.4.45                 Enzyme                                 

Name
melamine deaminase;
triA (gene name)
Class
Hydrolases;
Acting on carbon-nitrogen bonds, other than peptide bonds;
In cyclic amidines
Sysname
melamine aminohydrolase
Reaction(IUBMB)
(1) melamine + H2O = ammeline + NH3 [RN:R06968];
(2) ammeline + H2O = ammelide + NH3 [RN:R06969]
Reaction(KEGG)
R06968 R06969
Substrate
melamine [CPD:C08737];
H2O [CPD:C00001];
ammeline [CPD:C08733]
Product
ammeline [CPD:C08733];
NH3 [CPD:C00014];
ammelide [CPD:C08734]
Comment
The enzyme, isolated from the bacterium Acidovorax citrulli, performs the deamination of melamine 15-fold faster than the deamination of ammeline. It also has activity with 2-chloro-4,6-diamino-s-triazine, but has no activity toward halo-substituted triazine ring compounds such as atrazine (cf. EC 3.8.1.8, atrazine chlorohydrolase).
History
EC 3.5.4.45 created 2017
Pathway
ec00791  Atrazine degradation
ec01120  Microbial metabolism in diverse environments
Orthology
K21593  melamine deaminase
Reference
1  [PMID:11274097]
  Authors
Seffernick JL, de Souza ML, Sadowsky MJ, Wackett LP
  Title
Melamine deaminase and atrazine chlorohydrolase: 98 percent identical but functionally different.
  Journal
J Bacteriol 183:2405-10 (2001)
DOI:10.1128/JB.183.8.2405-2410.2001
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 3.5.4.45
IUBMB Enzyme Nomenclature: 3.5.4.45
ExPASy - ENZYME nomenclature database: 3.5.4.45
BRENDA, the Enzyme Database: 3.5.4.45

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