Entry
Name
Class
Hydrolases;
Acting on carbon-nitrogen bonds, other than peptide bonds;
In nitriles
BRITE hierarchy
Sysname
arylacetonitrile aminohydrolase
Reaction(IUBMB)
4-chlorophenylacetonitrile + 2 H2O = 4-chlorophenylacetate + NH3 [RN:
R04140 ]
Reaction(KEGG)
Substrate
Product
Comment
Requires thiol compounds. Also hydrolyses other 4-substituted phenylacetonitriles, thien-2-ylacetonitrile, tolylacetonitriles, and, more slowly, benzyl cyanide.
History
EC 3.5.5.5 created 1992
Orthology
Genes
Taxonomy
Reference
1
Authors
Mauger J, Nagasawa T, Yamada H.
Title
Occurrence of a novel nitrilase, arylacetonitrilase, in Alcaligenes faecalis JM3.
Journal
Arch Microbiol 155:1-6 (1990)
Reference
Authors
Nagasawa T, Mauger J, Yamada H
Title
A novel nitrilase, arylacetonitrilase, of Alcaligenes faecalis JM3. Purification and characterization.
Journal
Sequence
Other DBs
ExplorEnz - The Enzyme Database: 3.5.5.5
ExPASy - ENZYME nomenclature database: 3.5.5.5
BRENDA, the Enzyme Database: 3.5.5.5