KEGG ENZYME: 3.5.5.7

ENZYME: 3.5.5.7

Entry

EC 3.5.5.7                  Enzyme

Name

aliphatic nitrilase

Class

Hydrolases;
Acting on carbon-nitrogen bonds, other than peptide bonds;
In nitriles

Sysname

aliphatic nitrile aminohydrolase

Reaction(IUBMB)

R-CN + 2 H2O = R-COOH + NH3 [RN:R00540]

Reaction(KEGG)

R00540 > R05358

Substrate

R-CN [CPD:C00726];
H2O [CPD:C00001]

Product

R-COOH [CPD:C00060];
NH3 [CPD:C00014]

Comment

Preferentially hydrolyses aliphatic nitriles, some of which are apparently not substrates for other known nitrilases (EC 3.5.5.1). Substrates include crotononitrile, acrylonitrile and glutaronitrile.

History

EC 3.5.5.7 created 1999

Pathway

ec00643

Styrene degradation

ec01120

Microbial metabolism in diverse environments

Orthology

K01502

aliphatic nitrilase

Reference

1 [PMID:1390687]

Authors

Kobayashi M, Yanaka N, Nagasawa T, Yamada H

Title

Primary structure of an aliphatic nitrile-degrading enzyme, aliphatic nitrilase, from Rhodococcus rhodochrous K22 and expression of its gene and identification of its active site residue.

Journal

Biochemistry 31:9000-7 (1992)
DOI:10.1021/bi00152a042

Sequence

[ag:BAA02127]

Reference

2 [PMID:11380987]

Authors

Pace HC, Brenner C.

Title

The nitrilase superfamily: classification, structure and function.

Journal

Genome Biol 2:REVIEWS0001 (2001)
DOI:10.1186/gb-2001-2-1-reviews0001