KEGG   ENZYME: 3.6.1.65
Entry
EC 3.6.1.65                 Enzyme                                 

Name
(d)CTP diphosphatase;
(d)CTP pyrophosphohydrolase;
(d)CTP diphosphohydrolase;
nudG (gene name)
Class
Hydrolases;
Acting on acid anhydrides;
In phosphorus-containing anhydrides
Sysname
(deoxy)cytidine 5'-triphosphate diphosphohydrolase
Reaction(IUBMB)
(1) CTP + H2O = CMP + diphosphate [RN:R00515];
(2) dCTP + H2O = dCMP + diphosphate [RN:R01668]
Reaction(KEGG)
R00515 R01668
Substrate
CTP [CPD:C00063];
H2O [CPD:C00001];
dCTP [CPD:C00458]
Product
CMP [CPD:C00055];
diphosphate [CPD:C00013];
dCMP [CPD:C00239]
Comment
The enzyme, characterized from the bacterium Escherichia coli, is specific for the pyrimidine nucleotides CTP and dCTP. It also acts on 5-methyl-dCTP, 5-hydroxy-dCTP and 8-hydroxy-dGTP.
History
EC 3.6.1.65 created 2013
Orthology
K08320  (d)CTP diphosphatase
Genes
ECO: b1759(nudG)
ECJ: JW1748(nudG)
ECD: ECDH10B_1897(nudG)
EBW: BWG_1572(nudG)
ECOK: ECMDS42_1434(nudG)
ECE: Z2791
ECS: ECs2465
ECF: ECH74115_2479(nudG)
ETW: ECSP_2327(nudG)
ELX: CDCO157_2299
EOI: ECO111_2270(nudG)
EOJ: ECO26_2534(nudG)
EOH: ECO103_1952(nudG)
ESL: O3K_11165
ESO: O3O_14450
ESM: O3M_11145
ECK: EC55989_1927(nudG)
ECG: E2348C_1887(nudG)
EOK: G2583_2205(nudG)
ELH: ETEC_1791
ECW: EcE24377A_1983(nudG)
ECP: ECP_1705
ECOS: EC958_1980(nudG)
ECV: APECO1_829(ynjG)
ECX: EcHS_A1843(nudG)
ECM: EcSMS35_1430(nudG)
ECY: ECSE_1930
ECR: ECIAI1_1820(nudG)
ECQ: ECED1_1961(nudG)
EUM: ECUMN_2048(nudG)
ECT: ECIAI39_1295(nudG)
EOC: CE10_2039(nudG)
EBR: ECB_01728(nudG)
EBL: ECD_01728(nudG)
EBE: B21_01716(nudG)
EBD: ECBD_1885
ECI: UTI89_C1955(ynjG)
EIH: ECOK1_1880(nudG)
ECZ: ECS88_1811(nudG)
ECC: c2160
ELO: EC042_1924(nudG)
ESE: ECSF_1620
EKF: KO11_13935(nudG)
EAB: ECABU_c20160(nudG)
EDJ: ECDH1ME8569_1703(nudG)
ELW: ECW_m1928(nudG)
ELL: WFL_09460(nudG)
ELC: i14_1979
ELD: i02_1979
ELP: P12B_c1324(nudG)
ELF: LF82_1533(nudG)
ECOI: ECOPMV1_01857(nudG)
ECOJ: P423_09365
EFE: EFER_1306(nudG)
EAL: EAKF1_ch4290c(nudG)
STY: STY1813
STT: t1180
STM: STM1301
SEB: STM474_1305(nudg)
SENI: CY43_06625
SPT: SPA1543
SEK: SSPA1434
SEI: SPC_2431
SEC: SCH_1323(nudG)
SHB: SU5_01920
SENS: Q786_08750
SED: SeD_A2045
SEG: SG1815
SEL: SPUL_1115
SET: SEN1742
SENA: AU38_09015
SENO: AU37_09020
SENV: AU39_09025
SENQ: AU40_10075
SENL: IY59_09220
SEEP: I137_05130
SENE: IA1_06420
SBG: SBG_1154
SBZ: A464_1256
SFL: SF1466
SFX: S1581
SFV: SFV_1459
SFE: SFxv_1654(nudG)
SFN: SFy_2095
SFS: SFyv_2149
SFT: NCTC1_01577(nudG)
SSN: SSON_1397
SBO: SBO_1330
SDY: SDY_1516
ENC: ECL_02458
ECLX: LI66_08955
ECLY: LI62_09730
ECLZ: LI64_09020
ECLO: ENC_14560
EEC: EcWSU1_01770(nudG)
ESA: ESA_02159
CSK: ES15_2309(nudG)
CTU: CTU_18130(nudG)
KPN: KPN_01211(nudG)
KPU: KP1_2239(nudG)
KPP: A79E_2975
KPR: KPR_2256
KPJ: N559_3070
KPX: PMK1_03590(nudG)
KPNU: LI86_15960
KPNK: BN49_2345
KVA: Kvar_3103
KPE: KPK_3232(nudG)
KOX: KOX_18090
KOE: A225_2474
EAE: EAE_21650
EAR: CCG29605
CRO: ROD_12991(nudG)
CKO: CKO_01786
CAMA: F384_05870
EBT: EBL_c23440(nudG)
RTG: NCTC13098_04217(nudG)
REE: electrica_03020(nudG)
CLAP: NCTC11466_02677(nudG)
KIE: NCTC12125_01678(nudG)
METY: MRY16398_22170(nudG)
AHN: NCTC12129_02264(nudG)
EBF: D782_2568
YPE: YPO2167(nudG)
YPK: y2154
YPH: YPC_2144(nudG)
YPA: YPA_1524
YPN: YPN_1633
YPM: YP_1967(nudG)
YPG: YpAngola_A2341(nudG)
YPZ: YPZ3_1678(nudG)
YPD: YPD4_1905(nudG)
YPX: YPD8_1644(nudG)
YPW: CH59_3956(nudG)
YPJ: CH55_571(nudG)
YPV: BZ15_1373(nudG)
YPL: CH46_2946(nudG)
YPS: YPTB2093(nudG)
YPO: BZ17_371(nudG)
YPI: YpsIP31758_1977(nudG)
YPY: YPK_2086
YPB: YPTS_2157
YPQ: DJ40_212(nudG)
YPU: BZ21_1373(nudG)
YPR: BZ20_17(nudG)
YPC: BZ23_1656(nudG)
YPF: BZ19_1449(nudG)
YEN: YE2255(nudG)
YEY: Y11_11171
YEW: CH47_1682(nudG)
YET: CH48_3944(nudG)
YEE: YE5303_17571(nudG)
YAL: AT01_535(nudG)
YFR: AW19_1095(nudG)
YIN: CH53_3857(nudG)
YKR: CH54_567
YRO: CH64_459(nudG)
YRU: BD65_236(nudG)
SMAR: SM39_2189(nudG)
SMAC: SMDB11_1985(nudG)
SMW: SMWW4_v1c27290(nudG)
SPE: Spro_2716
SRL: SOD_c25580(nudG)
SPLY: Q5A_014175(nudG)
SMAF: D781_2464
SERF: L085_15165
SFJ: SAMEA4384070_2761(nudG)
SOF: NCTC11214_04164(nudG)
RAA: Q7S_10440
SGL: SG1362
SOD: Sant_1823(nudG)
ETR: ETAE_1495(nudG)
ETD: ETAF_1388
ETE: ETEE_3499
LRI: NCTC12151_01462(nudG)
PSHI: SAMEA2665130_2102(nudG_2)
FBL: Fbal_0702
TKM: TK90_2491
TVR: TVD_00210
SGN: SGRA_2437(nudG)
CTHI: THC_0949
 » show all
Reference
1  [PMID:11053429]
  Authors
O'Handley SF, Dunn CA, Bessman MJ
  Title
Orf135 from Escherichia coli Is a Nudix hydrolase specific for CTP, dCTP, and 5-methyl-dCTP.
  Journal
J Biol Chem 276:5421-6 (2001)
DOI:10.1074/jbc.M004100200
  Sequence
[eco:b1759]
Reference
2  [PMID:12509230]
  Authors
Fujikawa K, Kasai H
  Title
The oxidized pyrimidine ribonucleotide, 5-hydroxy-CTP, is hydrolyzed efficiently  by the Escherichia coli recombinant Orf135 protein.
  Journal
DNA Repair (Amst) 1:571-6 (2002)
DOI:10.1016/S1568-7864(02)00057-5
  Sequence
[eco:b1759]
Reference
3  [PMID:15381107]
  Authors
Kamiya H, Iida E, Harashima H
  Title
Important amino acids in the phosphohydrolase module of Escherichia coli Orf135.
  Journal
Biochem Biophys Res Commun 323:1063-8 (2004)
DOI:10.1016/j.bbrc.2004.08.201
  Sequence
[eco:b1759]
Reference
4  [PMID:15823026]
  Authors
Iida E, Satou K, Mishima M, Kojima C, Harashima H, Kamiya H
  Title
Amino acid residues involved in substrate recognition of the Escherichia coli Orf135 protein.
  Journal
Biochemistry 44:5683-9 (2005)
DOI:10.1021/bi048071o
  Sequence
[eco:b1759]
Other DBs
ExplorEnz - The Enzyme Database: 3.6.1.65
IUBMB Enzyme Nomenclature: 3.6.1.65
ExPASy - ENZYME nomenclature database: 3.6.1.65
BRENDA, the Enzyme Database: 3.6.1.65

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