KEGG   ENZYME: 3.6.1.70
Entry
EC 3.6.1.70                 Enzyme                                 
Name
guanosine-5'-diphospho-5'-[DNA] diphosphatase;
aprataxin;
pp5'G5'DNA diphosphatase;
pp5'G5'-DNA guanylate hydrolase;
APTX (gene name);
HNT3 (gene name)
Class
Hydrolases;
Acting on acid anhydrides;
In phosphorus-containing anhydrides
Sysname
guanosine-5'-diphospho-5'-[DNA] hydrolase (guanosine 5'-phosphate-forming)
Reaction(IUBMB)
guanosine-5'-diphospho-5'-[DNA] + H2O = phospho-5'-[DNA] + GMP
Substrate
guanosine-5'-diphospho-5'-[DNA];
H2O [CPD:C00001]
Product
phospho-5'-[DNA];
GMP [CPD:C00144]
Comment
Aprataxin is a DNA-binding protein that catalyses (among other activities) the 5' decapping of Gpp-DNA (formed by homologs of RtcB3 from the bacterium Myxococcus xanthus). The enzyme binds the guanylate group to a histidine residue at its active site, forming a covalent enzyme-nucleotide phosphate intermediate, followed by the hydrolysis of the guanylate from the nucleic acid and eventual release. The enzyme forms a 5'-phospho terminus that can be efficiently joined by "classical" ligases. The enzyme also possesses the activitiy of EC 3.6.1.71, adenosine-5'-diphospho-5'-[DNA] diphosphatase and EC 3.6.1.72, DNA-3'-diphospho-5'-guanosine diphosphatase.
History
EC 3.6.1.70 created 2017 as EC 3.1.11.8, transferred 2019 to EC 3.6.1.70
Orthology
K10863  aprataxin
Genes
HSA54840(APTX)
PTR465037(APTX)
PPS100972572(APTX)
GGO101143636(APTX)
PON100439490(APTX)
NLE100592983(APTX)
MCC702671(APTX)
MCF102135987(APTX)
MTHB126937408
CSAB103219297(APTX)
CATY105590087(APTX)
PANU101024645(APTX)
TGE112608645(APTX)
RRO104674274(APTX)
RBB108518241(APTX)
TFN117068911(APTX)
PTEH111529209(APTX)
CANG105526260(APTX)
CJC100410996(APTX)
SBQ101046890(APTX)
CSYR103270027(APTX)
MMUR105874377(APTX)
LCAT123646225(APTX)
PCOQ105820541(APTX)
OGA100962727(APTX)
MMU66408(Aptx)
MCAL110292499(Aptx)
MPAH110338795(Aptx)
RNO259271(Aptx)
MCOC116095822(Aptx)
MUN110566705(Aptx)
CGE100774231(Aptx)
MAUA101825422(Aptx)
PLEU114703261(Aptx)
MORG121458334(Aptx)
MFOT126503631
AAMP119826722(Aptx)
NGI103741597(Aptx)
HGL101719882(Aptx)
CPOC100722168(Aptx)
CCAN109681409(Aptx)
DORD105993389(Aptx)
DSP122123400(Aptx)
NCAR124964881
OCU100351115 103348750
OPI101523019(APTX)
TUP102475725(APTX)
GVR103593729(APTX)
CFA442943(APTX)
CLUD112650187(APTX)
VVP112927557(APTX)
VLG121494579(APTX)
AML100477925(APTX)
UMR103667027(APTX)
UAH113260533(APTX)
UAR123781088(APTX)
ELK111145935
LLV125083990
MPUF101687373(APTX)
ORO101386234(APTX)
EJU114218165(APTX)
ZCA113934489(APTX)
MLX118018030(APTX)
NSU110581448(APTX)
LWW102726820(APTX)
FCA101097680(APTX)
PYU121034559(APTX)
PBG122474586(APTX)
LRUF124501277
PTG102950944(APTX)
PPAD109252050(APTX)
AJU106976035(APTX)
HHV120241765(APTX)
BTA359714(APTX)
BOM102268362(APTX)
BIU109563163(APTX)
BBUB102391016(APTX)
BBIS104988714(APTX)
CHX102186869(APTX)
OAS101105385(APTX)
ODA120860350 120866131
CCAD122431966(APTX)
SSC387596(APTX)
CFR102516533(APTX)
CBAI105078742(APTX)
CDK105085570(APTX)
VPC102530854(APTX)
BACU102997771(APTX)
LVE103085186(APTX)
OOR101283923(APTX)
DLE111163193(APTX)
PCAD102980098(APTX)
PSIU116755262(APTX)
ECB100068091(APTX)
EPZ103555028(APTX)
EAI106841467(APTX)
MYB102241189(APTX)
MYD102760132(APTX)
MMYO118667400(APTX)
MLF102436912(APTX)
MNA107544998(APTX)
PKL118716418(APTX)
HAI109371794(APTX)
DRO112304535(APTX)
SHON118979822(APTX)
AJM119060017(APTX)
PDIC114509072(APTX)
PHAS123820200(APTX)
MMF118630120(APTX)
RFQ117031482(APTX)
PALE102880269(APTX)
PGIG120612920(APTX)
PVP105306410(APTX)
RAY107502755(APTX)
MJV108404115(APTX)
TOD119230949(APTX) 119253332
SARA101557569(APTX)
LAV100660757(APTX)
TMU101342573
ETF101641453(APTX)
DNM101412215(APTX)
MDO100020823(APTX)
GAS123231259(APTX)
SHR100934939(APTX)
PCW110204302 110220574(APTX)
OAA100075506(APTX)
GGA395173(APTX)
PCOC116239465(APTX)
MGP100543078 104915326
CJO107306647(APTX)
NMEL110389533(APTX)
APLA101792244(APTX)
ACYG106040205(APTX)
AFUL116501093(APTX)
TGU100226520(APTX)
LSR110474048(APTX)
SCAN103821836(APTX)
PMOA120513600(APTX)
OTC121332676(APTX)
PRUF121363767(APTX)
GFR102042023(APTX)
FAB101807349(APTX)
PHI102109622(APTX)
PMAJ107216303(APTX)
CCAE111941318(APTX)
CCW104689793(APTX)
CBRC103616367(APTX)
ETL114057879(APTX)
ZAB102065002(APTX)
ACHL103811265(APTX)
FPG101917451(APTX)
FCH102057105(APTX)
CLV102086630(APTX)
EGZ104123062(APTX)
NNI104022636(APTX)
PLET104615124(APTX)
PCRI104030234(APTX)
PCAO104044133(APTX)
ACUN113490307(APTX)
TALA104365643(APTX)
PADL103921041(APTX)
AFOR103903770(APTX)
ACHC115337047(APTX)
HALD104317211(APTX)
HLE104838394(APTX)
AGEN126036524
GCL127027262
CCRI104167170(APTX)
CSTI104556112(APTX)
CMAC104483870(APTX)
MUI104542617(APTX)
FGA104074319(APTX)
GSTE104252030 104258213(APTX)
LDI104350347(APTX)
MNB103770359(APTX)
OHA104338450(APTX)
NNT104400753(APTX)
DPUB104299410(APTX)
PGUU104465194(APTX)
ACAR104529960(APTX)
CPEA104391678(APTX)
AVIT104278130(APTX)
CVF104282564(APTX)
CUCA104059495(APTX)
TEO104374633(APTX)
BRHI104490040(APTX)
AAM106495965(APTX)
AROW112975299(APTX)
NPD112948931(APTX)
TGT104578637(APTX)
DNE112989808(APTX)
SCAM104144367(APTX)
ASN102386580(APTX)
AMJ102559093(APTX)
CPOO109323595(APTX)
GGN109297436(APTX)
PSS102460401(APTX)
CMY102939347(APTX)
CPIC101943978(APTX)
TST117878704(APTX)
CABI116828220(APTX)
MRV120393934 120407752
ACS100558032(aptx)
PVT110087361(APTX)
SUND121921205(APTX)
PBI103066205(APTX)
PMUR107285612(APTX)
CTIG120303828(APTX)
TSR106548867(APTX)
PGUT117661721(APTX)
VKO123017519(APTX)
PMUA114587702(APTX)
ZVI118097514(APTX)
GJA107118361(APTX)
STOW125436187(APTX)
XLA398657(aptx.L)
XTR403092(aptx)
NPR108798251(APTX)
RTEM120915189(APTX)
BBUF120989625(APTX)
BGAR122946091(APTX)
DRE405797(aptx)
SRX107746089 107757069
SANH107657991(aptx)
SGH107570816(aptx)
CCAR109051875 109051898 109065131
CAUA113062249(aptx)
PPRM120489166(aptx)
MAMB125271853(aptx)
IPU108260610(aptx)
PHYP113524515(aptx)
SMEO124381552(aptx)
AMEX103046803(aptx)
EEE113583868(aptx)
TRU445995(aptx)
TNGGSTEN00020002G001
LCO104930177(aptx)
NCC104954692(aptx)
CGOB115007814(aptx)
ELY117255455(aptx)
EFO125885520(aptx)
PLEP121942280(aptx)
SLUC116034375(aptx)
ECRA117955575(aptx)
ESP116699444(aptx)
PFLV114569374(aptx)
GAT120825231(aptx)
PPUG119218279(aptx)
MSAM119898995(aptx)
CUD121508519(aptx)
ALAT119020302(aptx)
MZE101466233(aptx)
ONL100708465(aptx)
OAU116334497(aptx)
OLA101162025(aptx)
OML112157141(aptx)
XMA102232898(aptx)
XCO114144368(aptx)
XHE116719650(aptx)
PRET103465988(aptx)
PFOR103137845 103137850
PLAI106943046 106943049
PMEI106911873 106919284
GAF122829406(aptx)
CVG107097703(aptx)
CTUL119774729(aptx)
GMU124868597(aptx)
NFU107375251(aptx)
KMR108237716(aptx)
ALIM106517163(aptx)
NWH119408079(aptx)
AOCE111579518(aptx)
MCEP125003494(aptx)
CSEM103383834(aptx)
POV109637835(aptx)
SSEN122770473(aptx)
HHIP117766804(aptx)
HSP118123134(aptx)
LCF108896493
SDU111231416(aptx)
SLAL111664047(aptx)
XGL120800216(aptx)
HCQ109519905(aptx)
BPEC110165402(aptx)
MALB109961360(aptx)
BSPL114858911(aptx)
SASA100194567(aptx)
STRU115164986(aptx)
OTW112248313(aptx)
OMY100136186(aptx)
OGO124034660(aptx)
ONE115111255(aptx)
SALP111975108(aptx)
SNH120044128(aptx)
CCLU121584387(aptx)
ELS105014574(aptx)
SFM108925927(aptx)
PKI111844213(aptx)
AANG118226770(aptx)
LOC102690326(aptx)
PSPA121296574(aptx)
ARUT117408685(aptx)
PSEX120532292(aptx)
LCM102345286(APTX)
CMK103187207(aptx)
RTP109926966(aptx)
BFO118428322
BBEL109477827
CIN445730(aptx)
SCLV120333816
SPU592796
APLC110981744
SKO102800978
DMEDmel_CG5316(CG5316)
DER6554045 6554047
DSE6619074
DSIDsimw501_GD19289(Dsim_GD19289)
DYADyak_GE25605 Dyak_GE27392 Dyak_GE28076 Dyak_GE29197
DSR110188279
DPO4803259
DPE6594229
DMN108153848 117187387
DWI111519400 6650256
DGR116806024 116806025 116806026 116806027 116806028 116806306 122322685 122322686 122322689 6569964
DMODmoj_GI16920 Dmoj_GI23101 Dmoj_GI23102
DAZ108608649 108614288 108614368
DNV108659192 108660069
DHE111596201 111596208
CCAT101462718
MDE101891634
SCAC106087202
LCQ111683105
AGAAgaP_AGAP004307 AgaP_AGAP012563
ACOZ120951911 120961674
AARA120897054 120901540
ASTE118502892 118504672
AAG5565705
AALB109623040
CQUCpipJ_CPIJ001673 CpipJ_CPIJ001675
CPII120418370 120418385
CNS116343672
AME408601
ACER107994706
ALAB122718623
BIM100747480
BBIF117204527
BVK117235196
BVAN117153638
BTER100644640
BPYO122569562
CCAL108629642
OBB114875389
MGEN117223654
NMEA116430515
CGIG122395240
SOC105202804
MPHA105835091
AEC105144914
PBAR105426048
VEM105561077
HST105186905
CFO105258852
FEX115234801
LHU105672859
PGC109859789
OBO105275839
PCF106788572
PFUC122514504 122520652
VPS122628408
NVI100115496
TPRE106652645
LHT122508514
CCIN107264919
TCA658572
DPA109535577
ATD109596255 109607377
CSET123306810
AGB108903797
LDC111503849
NVL108559963
APLN108734079 108740333
PPYR116180344
OTU111427537
BMOR101744383
BMAN114241482
MSEX115439968
DPLKGM_213605
BANY112043144
PMAC106719089
PPOT106109714
PXU106121456
PRAP111003738
PBX123715748
PNAP125057287
ZCE119840816
CCRC123694330
HAW110379623
HZE124635171
TNL113501361
SLIU111359010
OFU114361662
PXY105386421
CFEL113366552
API100569003
DNX107166542
AGS114120898
RMD113549244
BTAB109035556
CLEC106663263
HHAL106690133
NLU111049854 120348853
PHUPhum_PHUM320100
FOC113204005
TPAL117651313
ZNE110834505
CSEC111867597
SGRE126354777
DMK116917474
PVM113816347
PJA122252788
PCHN125036254
HAME121856541
PCLA123755880
PTRU123507555
HAZT108673937
EAF111697217
LSM121119440 121130830
VJA111265003
CSCU111627534
PTEP107449666
SDM118191500
HROHELRODRAFT_62577
LGILOTGIDRAFT_186969
PCAN112555301
BGT106056408
GAE121367307
HRF124110391
HRJ124278883
CRG105322078
MYI110446209
PMAX117342125
MMER123538359
OBI106880390
OSN115222296
LAK106154458 106163822
OVIT265_08155
HMG100206030
TADTRIADDRAFT_18245
AQU100640625
ATHAT5G01310(APTX)
ALY9307002
CRB17882450
CSAT104708982 104734171 104768332
BRP103847505
BOE106319492
RSZ108818268
THJ104816650
CPAP110809488
CIT102619121
CICCICLE_v10011130mg
PVY116115203 116138719 116138755
MINC123223030
TCC18597500
GRA105779441
GHI107899563 121215903
GAB108460140
DZI111295225 111310664
EGR104423355
GMX100786762
GSJ114415910
PVUPHAVU_010G073200g
VRA106772894
VAR108346640
VUN114167226
CCAJ109806112
APRC113849311
MTR11415306
CAM101510807
PSAT127093482
LJALj0g3v0316429.1(Lj0g3v0316429.1)
ADU107475347
AIP107625784
AHF112728031 112750636
LANG109361051
FVE101306149
RCN112192537
PPER18775191
PMUM103341550
PAVI110768433
PDUL117632708
MDM103425917
PXB103965126 103965157
ZJU107430895
MNT21395270
CSV101216867
CMO103486269
BHJ120091955
MCHA111015060
CMAX111470189
CMOS111452802
CPEP111801005
RCU8283140
HBR110662752
MESC110623086
POP7460370
PEU105141363
PALZ118037517 118045838
JRE108995190
CILL122279587
QSU111985592 112021524 112040136
QLO115957312
TWL119987513
VVI100259037
VRI117920967
SLY101257206
SPEN107031205
SOT102578604
SSTN125871177
CANN107842536
NTA107789889 107826076
NSY104236796
NTO104118789
NAU109219272
INI109181766
ITR116005567
SIND105174593
OEU111392531 111392550
EGT105953116
SSPL121795633 121800888
APAN127252118
HAN110896008
ECAD122594469
LSV111908638
CCAV112504614
DCR108208832
CSIN114261388
BVG104892527
SOE110791642
CQI110710710 110733905
NNU104594951
MING122067314
TSS122671691
PSOM113291612 113310226 113322015
OSA4332518
DOSAOs03t0293400-01(Os03g0293400)
OBR102709720
BDI100839576
ATS109733434
TDC119285405 119296426
TAES123085582 123092448 123097769
TUA125552066 125552070
LPER127295805
SBI8084030
ZMA103634500
SITA101776352
SVS117836048
PVIR120652712 120687912
PHAI112877291
PDA103706712
EGU105057122
MUS103974008
ZOF122052160 122056649
DCT110096109
PEQ110028380
AOF109842914
NCOL116254085
ATR18444741
SMOSELMODRAFT_104482 SELMODRAFT_89021
PPP112288900
CRECHLRE_06g297904v5
VCNVOLCADRAFT_120448
MNGMNEG_4264
CSLCOCSUDRAFT_42402
CVRCHLNCDRAFT_136358
BPGBathy05g04070
MISMICPUN_84536
MPPMICPUCDRAFT_28995
SCEYOR258W(HNT3)
SEUBDI49_5079
SPAOSPAR_O03740
AGOAGOS_AAL112C
KLAKLLA0_C09625g
KMXKLMA_30465(HNT3)
LTHKLTH0D11968g
VPOKpol_1064p21
ZROZYRO0F11924g
CGRCAGL0K08932g
NCSNCAS_0B01160(NCAS0B01160)
NDINDAI_0E01030(NDAI0E01030)
TBLTBLA_0B03650(TBLA0B03650)
TGBHG536_0A08080
TDLTDEL_0A06520(TDEL0A06520)
KAFKAFR_0A04000(KAFR0A04000)
KNGKNAG_0G02710(KNAG0G02710)
ZMKHG535_0A06400
DHADEHA2F20284g
PICPICST_33883(HNT3)
PGUPGUG_00302
SPAASPAPADRAFT_59734
LELLELG_00273
CALCAALFM_CR03440WA(CaO19.2376) CAALFM_CR03590CA(CaO19.4383)
CTPCTRG_00756
COTCORT_0A02110
CDUCD36_28600 CD36_28780
CTENCANTEDRAFT_96055
YLIYALI0E21890g
CLUCLUG_03957
CLUSA9F13_01g01694
CAURCJI97_002700
SLBAWJ20_1739(HNT3)
OPAHPODL_02803
SLUDSCDLUD_002192
NCRNCU06572
NTENEUTE1DRAFT136002(NEUTE1DRAFT_136002)
SMPSMAC_04695
PANPODANS72p067
TTTTHITE_2044821
MTMMYCTH_2310466
CTHRCTHT_0036990
MGRMGG_07058
PPEIPpBr36_01684
TMNUCRPA7_1512
SSCKSPSK_09595
FPUFPSE_04109
FVRFVEG_16236
FOXFOXG_01657
NHENECHADRAFT_49288
TRETRIREDRAFT_53660
TRRM419DRAFT_69637
MAWMAC_00501
MAJMAA_07304
PLJVFPFJ_07836
VALVDBG_00048
VDAVDAG_08337
CFJCFIO01_07552
CLUPCLUP02_08347
CHIGCH63R_04441
SAPOSAPIO_CDS10419
ELAUCREL1_4929
PFYPFICI_07637
SSLSS1G_11675
BFUBCIN_08g06920(Bchnt3)
MBEMBM_01023
PSCOLY89DRAFT_656717
GLZGLAREA_06607
ANIAN2139.2
AFMAFUA_2G16130
ACTACLA_075090
NFINFIA_091390
AORAO090012000185
ANGANI_1_1686134(An15g06480)
AFVAFLA_001905
ALUCAKAW2_60132S(HNT3)
ACHEACHE_60390S(HNT3)
APUUAPUU_20435S(HNT3)
PCSPc21g01930
PDPPDIP_80490
TMFPMAA_038010
TRGTRUGW13939_05042
CIMCIMG_09530
CPWCPC735_018800
UREUREG_04789
PBLPAAG_06653
PBNPADG_01109
ABEARB_03228
TVETRV_02456
AJEHCAG_06451
BGHBDBG_02719
PNOSNOG_10141
PTEPTT_12333
BZECOCCADRAFT_6096
BORCOCMIDRAFT_35169
AALTCC77DRAFT_1006560
ZTRMYCGRDRAFT_55061
PFJMYCFIDRAFT_186479
FFUCLAFUR5_03493
BCOMBAUCODRAFT_32290
NPAUCRNP2_8933
TMLGSTUM_00001306001
SPOSPCC18.09c
CNECNC04800
CNBCNBC2380
CNGCNAG_02862
CGICGB_C7100C
TMSTREMEDRAFT_59866
TASAA1Q1_02217
PPLPOSPLDRAFT_97392
TVSTRAVEDRAFT_136817
DSQDICSQDRAFT_48615
SHSSTEHIDRAFT_108538
HIRHETIRDRAFT_309237
PSQPUNSTDRAFT_114838 PUNSTDRAFT_28176
ADLAURDEDRAFT_19811
FMEFOMMEDRAFT_109677
GTRGLOTRDRAFT_56862
CCICC1G_04299
ABPAGABI1DRAFT122637(AGABI1DRAFT_122637)
ABVAGABI2DRAFT187227(AGABI2DRAFT_187227)
MRRMoror_8948
MOREE1B28_008668
SCMSCHCO_02605771(SCHCODRAFT_02605771)
CPUTCONPUDRAFT_117509
SLASERLADRAFT_357670
WSEWALSEDRAFT_70138
WICJ056_003041
UMAUMAG_02669
PFPPFL1_03844
MGLMGL_0877
MRTMRET_2631
MBRMONBRDRAFT_29002
SREPTSG_12341
DDIDDB_G0272839
DPPDICPUDRAFT_153568
DFADFA_01715
ACANACA1_270320
SPARSPRG_06657
EHXEMIHUDRAFT_424881 EMIHUDRAFT_434812
GTTGUITHDRAFT_134117
TBRTb927.3.3710
TBGTbgDal_III4010
TCR506989.50 510149.100
LMALMJF_29_0640
LIFLINJ_29_0670
LDOLDBPK_290670
LMILMXM_08_29_0640
LBZLBRM_29_0650
LPANLPMP_290630
 » show all
Reference
1  [PMID:26350128]
  Authors
Maughan WP, Shuman S
  Title
Characterization of 3'-Phosphate RNA Ligase Paralogs RtcB1, RtcB2, and RtcB3 from Myxococcus xanthus Highlights DNA and RNA 5'-Phosphate Capping Activity of RtcB3.
  Journal
J Bacteriol 197:3616-24 (2015)
DOI:10.1128/JB.00631-15
Other DBs
ExplorEnz - The Enzyme Database: 3.6.1.70
IUBMB Enzyme Nomenclature: 3.6.1.70
ExPASy - ENZYME nomenclature database: 3.6.1.70
BRENDA, the Enzyme Database: 3.6.1.70

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