KEGG   ENZYME: 3.6.1.71
Entry
EC 3.6.1.71                 Enzyme                                 

Name
adenosine-5'-diphospho-5'-[DNA] diphosphatase;
aprataxin;
5'-App5'-DNA adenylate hydrolase;
APTX (gene name);
HNT3 (gene name)
Class
Hydrolases;
Acting on acid anhydrides;
In phosphorus-containing anhydrides
Sysname
adenosine-5'-diphospho-5'-[DNA] hydrolase (adenosine 5'-phosphate-forming)
Reaction(IUBMB)
(1) adenosine-5'-diphospho-5'-[DNA] + H2O = AMP + phospho-5'-[DNA];
(2) adenosine-5'-diphospho-5'-(ribonucleotide)-[DNA] + H2O = AMP + 5'-phospho-(ribonucleotide)-[DNA]
Substrate
adenosine-5'-diphospho-5'-[DNA];
H2O [CPD:C00001];
adenosine-5'-diphospho-5'-(ribonucleotide)-[DNA]
Product
AMP [CPD:C00020];
phospho-5'-[DNA];
5'-phospho-(ribonucleotide)-[DNA]
Comment
Aprataxin is a DNA-binding protein involved in different types of DNA break repair. The enzyme acts (among other activities) on abortive DNA ligation intermediates that contain an adenylate covalently linked to the 5'-phosphate DNA terminus. It also acts when the adenylate is covalently linked to the 5'-phosphate of a ribonucleotide linked to a DNA strand, which is the result of abortive ligase activty on products of EC 3.1.26.4, ribonuclease H, an enzyme that cleaves RNA-DNA hybrids on the 5' side of the ribonucleotide found in the 5'-RNA-DNA-3' junction. Aprataxin binds the adenylate group to a histidine residue within the active site, followed by its hydrolysis from the nucleic acid and eventual release, leaving a 5'-phosphate terminus that can be efficiently rejoined. The enzyme also possesses the activities of EC 3.6.1.70, guanosine-5'-diphospho-5'-[DNA] diphosphatase, and EC 3.6.1.72, DNA-3'-diphospho-5'-guanosine diphosphatase.
History
EC 3.6.1.71 created 2017 as EC 3.1.11.7, transferred 2019 to EC 3.6.1.71
Orthology
K10863  aprataxin
Genes
HSA: 54840(APTX)
PTR: 465037(APTX)
PPS: 100972572(APTX)
GGO: 101143636(APTX)
PON: 100439490(APTX)
NLE: 100592983(APTX)
MCC: 702671(APTX)
MCF: 102135987(APTX)
CSAB: 103219297(APTX)
CATY: 105590087(APTX)
PANU: 101024645(APTX)
RRO: 104674274(APTX)
RBB: 108518241(APTX)
TFN: 117068911(APTX)
PTEH: 111529209(APTX)
CJC: 100410996(APTX)
SBQ: 101046890(APTX)
MMUR: 105874377(APTX)
MMU: 66408(Aptx)
MCAL: 110292499(Aptx)
MPAH: 110338795(Aptx)
RNO: 259271(Aptx)
MCOC: 116095822(Aptx)
MUN: 110566705(Aptx)
CGE: 100774231(Aptx)
PLEU: 114703261(Aptx)
NGI: 103741597(Aptx)
HGL: 101719882(Aptx)
CCAN: 109681409(Aptx)
OCU: 100351115(APTX) 103348750
OPI: 101523019(APTX)
TUP: 102475725(APTX)
CFA: 442943(APTX)
VVP: 112927557(APTX)
VLG: 121494579(APTX)
AML: 100477925(APTX)
UMR: 103667027(APTX)
UAH: 113260533(APTX)
ORO: 101386234(APTX)
ELK: 111145935
MPUF: 101687373(APTX)
EJU: 114218165(APTX)
MLX: 118018030(APTX)
FCA: 101097680(APTX)
PYU: 121034559(APTX)
PBG: 122474586(APTX)
PTG: 102950944(APTX)
PPAD: 109252050(APTX)
AJU: 106976035(APTX)
HHV: 120241765(APTX)
BTA: 359714(APTX)
BOM: 102268362(APTX)
BIU: 109563163(APTX)
BBUB: 102391016(APTX)
CHX: 102186869(APTX)
OAS: 101105385(APTX)
CCAD: 122431966(APTX)
SSC: 387596(APTX)
CFR: 102516533(APTX)
CBAI: 105078742(APTX)
CDK: 105085570(APTX)
BACU: 102997771(APTX)
LVE: 103085186(APTX)
OOR: 101283923(APTX)
DLE: 111163193(APTX)
PCAD: 102980098(APTX)
ECB: 100068091(APTX)
EPZ: 103555028(APTX)
EAI: 106841467(APTX)
MYB: 102241189(APTX)
MYD: 102760132(APTX)
MMYO: 118667400(APTX)
MNA: 107544998(APTX)
PKL: 118716418(APTX)
HAI: 109371794(APTX)
DRO: 112304535(APTX)
SHON: 118979822(APTX)
AJM: 119060017(APTX)
MMF: 118630120(APTX)
PALE: 102880269(APTX)
PGIG: 120612920(APTX)
RAY: 107502755(APTX)
MJV: 108404115(APTX)
TOD: 119230949(APTX) 119253332
LAV: 100660757(APTX)
TMU: 101342573
MDO: 100020823(APTX)
SHR: 100934939(APTX)
PCW: 110204302 110220574(APTX)
OAA: 100075506(APTX)
GGA: 395173(APTX)
PCOC: 116239465(APTX)
CJO: 107306647(APTX)
NMEL: 110389533(APTX)
APLA: 101792244(APTX)
ACYG: 106040205(APTX)
TGU: 100226520(APTX)
LSR: 110474048(APTX)
SCAN: 103821836(APTX)
PMOA: 120513600(APTX)
OTC: 121332676(APTX)
PRUF: 121363767(APTX)
GFR: 102042023(APTX)
FAB: 101807349(APTX)
PHI: 102109622(APTX)
PMAJ: 107216303(APTX)
CCAE: 111941318(APTX)
CCW: 104689793(APTX)
ETL: 114057879(APTX)
FPG: 101917451(APTX)
FCH: 102057105(APTX)
CLV: 102086630(APTX)
EGZ: 104123062(APTX)
NNI: 104022636(APTX)
ACUN: 113490307(APTX)
PADL: 103921041(APTX)
AAM: 106495965(APTX)
AROW: 112975299(APTX)
NPD: 112948931(APTX)
DNE: 112989808(APTX)
ASN: 102386580(APTX)
AMJ: 102559093(APTX)
CPOO: 109323595(APTX)
GGN: 109297436(APTX)
PSS: 102460401(APTX)
CMY: 102939347(APTX)
CPIC: 101943978(APTX)
TST: 117878704(APTX)
CABI: 116828220(APTX)
ACS: 100558032(aptx)
PVT: 110087361(APTX)
PBI: 103066205(APTX)
PMUR: 107285612(APTX)
TSR: 106548867(APTX)
PGUT: 117661721(APTX)
PMUA: 114587702(APTX)
ZVI: 118097514(APTX)
GJA: 107118361(APTX)
XLA: 398657(aptx.L)
XTR: 403092(aptx)
NPR: 108798251(APTX)
DRE: 405797(aptx)
SANH: 107657991(aptx)
SGH: 107570816(aptx)
CAUA: 113062249(aptx)
IPU: 108260610(aptx)
PHYP: 113524515(aptx)
AMEX: 103046803(aptx)
EEE: 113583868(aptx)
TRU: 445995(aptx)
LCO: 104930177(aptx)
NCC: 104954692(aptx)
CGOB: 115007814(aptx)
ELY: 117255455(aptx)
PLEP: 121942280(aptx)
SLUC: 116034375(aptx)
ECRA: 117955575(aptx)
PFLV: 114569374(aptx)
GAT: 120825231(aptx)
PPUG: 119218279(aptx)
MSAM: 119898995(aptx)
CUD: 121508519(aptx)
MZE: 101466233(aptx)
ONL: 100708465(aptx)
OAU: 116334497(aptx)
OLA: 101162025(aptx)
OML: 112157141(aptx)
XMA: 102232898(aptx)
XCO: 114144368(aptx)
XHE: 116719650(aptx)
PRET: 103465988(aptx)
CVG: 107097703(aptx)
CTUL: 119774729(aptx)
NFU: 107375251(aptx)
KMR: 108237716(aptx)
ALIM: 106517163(aptx)
AOCE: 111579518(aptx)
CSEM: 103383834(aptx)
POV: 109637835(aptx)
LCF: 108896493(aptx)
SDU: 111231416(aptx)
SLAL: 111664047(aptx)
XGL: 120800216(aptx)
HCQ: 109519905(aptx)
BPEC: 110165402(aptx)
MALB: 109961360(aptx)
SASA: 100194567(aptx)
OTW: 112248313(aptx)
OMY: 100136186(aptx)
SALP: 111975108(aptx)
SNH: 120044128(aptx)
ELS: 105014574(aptx)
SFM: 108925927(aptx)
PKI: 111844213(aptx)
AANG: 118226770(aptx)
LOC: 102690326(aptx)
PSPA: 121296574(aptx)
ARUT: 117408685(aptx)
LCM: 102345286(APTX)
CMK: 103187207(aptx)
RTP: 109926966(aptx)
BFO: 118428322
BBEL: 109477827
CIN: 445730(aptx)
SCLV: 120333816
SPU: 592796
APLC: 110981744
SKO: 102800978
DME: Dmel_CG5316(CG5316)
DSE: 6619074
DSI: Dsimw501_GD19289(Dsim_GD19289)
DSR: 110188279
DPE: 6594229
CCAT: 101462718
MDE: 101891634
SCAC: 106087202
LCQ: 111683105
AAG: 5565705
AALB: 109623040
AME: 408601
ACER: 107994706
BIM: 100747480
BBIF: 117204527
BVK: 117235196
BVAN: 117153638
BTER: 100644640
CCAL: 108629642
OBB: 114875389
MGEN: 117223654
NMEA: 116430515
CGIG: 122395240
SOC: 105202804
MPHA: 105835091
AEC: 105144914
PBAR: 105426048
VEM: 105561077
HST: 105186905
CFO: 105258852
FEX: 115234801
LHU: 105672859
PGC: 109859789
OBO: 105275839
PCF: 106788572
NVI: 100115496
TPRE: 106652645
CCIN: 107264919
TCA: 658572
AGB: 108903797
LDC: 111503849
NVL: 108559963
PPYR: 116180344
OTU: 111427537
BMOR: 101744383
BMAN: 114241482
MSEX: 115439968
BANY: 112043144
PMAC: 106719089
PPOT: 106109714
PXU: 106121456
PRAP: 111003738
ZCE: 119840816
HAW: 110379623
TNL: 113501361
PXY: 105386421
API: 100569003
DNX: 107166542
AGS: 114120898
RMD: 113549244
BTAB: 109035556
CLEC: 106663263
HHAL: 106690133
FOC: 113204005
ZNE: 110834505
CSEC: 111867597
DMK: 116917474
PVM: 113816347
HAME: 121856541
HAZT: 108673937
EAF: 111697217
VJA: 111265003
CSCU: 111627534
PTEP: 107449666
SDM: 118191500
PCAN: 112555301
BGT: 106056408
GAE: 121367307
CRG: 105322078
MYI: 110446209
PMAX: 117342125
OBI: 106880390
OSN: 115222296
HMG: 100206030
AQU: 100640625
ATH: AT5G01310(APTX)
ALY: 9307002
CRB: 17882450
BRP: 103847505
BOE: 106319492
RSZ: 108818268
THJ: 104816650
CPAP: 110809488
CIT: 102619121
MINC: 123223030
TCC: 18597500
GRA: 105779441
GAB: 108460140
EGR: 104423355
GMX: 100786762
GSJ: 114415910
VRA: 106772894
VAR: 108346640
VUN: 114167226
CCAJ: 109806112
APRC: 113849311
CAM: 101510807
LJA: Lj0g3v0316429.1(Lj0g3v0316429.1)
ADU: 107475347
AIP: 107625784
LANG: 109361051
FVE: 101306149
RCN: 112192537
PPER: 18775191
PMUM: 103341550
PAVI: 110768433
PDUL: 117632708
MDM: 103425917
ZJU: 107430895
MNT: 21395270
CSV: 101216867
CMO: 103486269
BHJ: 120091955
MCHA: 111015060
CMAX: 111470189
CMOS: 111452802
CPEP: 111801005
RCU: 8283140
HBR: 110662752
MESC: 110623086
POP: 7460370
PEU: 105141363
JRE: 108995190
QLO: 115957312
TWL: 119987513
VVI: 100259037
VRI: 117920967
SLY: 101257206
SPEN: 107031205
SOT: 102578604
CANN: 107842536
NSY: 104236796
NTO: 104118789
NAU: 109219272
INI: 109181766
ITR: 116005567
SIND: 105174593
EGT: 105953116
HAN: 110896008
ECAD: 122594469
LSV: 111908638
CCAV: 112504614
DCR: 108208832
CSIN: 114261388
BVG: 104892527
SOE: 110791642
NNU: 104594951
MING: 122067314
NCOL: 116254085
OSA: 4332518
DOSA: Os03t0293400-01(Os03g0293400)
OBR: 102709720
BDI: 100839576
ATS: 109733434
SBI: 8084030
ZMA: 103634500
SITA: 101776352
PHAI: 112877291
PDA: 103706712
EGU: 105057122
MUS: 103974008
DCT: 110096109
PEQ: 110028380
AOF: 109842914
ATR: 18444741
PPP: 112288900
MNG: MNEG_4264
SCE: YOR258W(HNT3)
KMX: KLMA_30465(HNT3)
NCS: NCAS_0B01160(NCAS0B01160)
NDI: NDAI_0E01030(NDAI0E01030)
TBL: TBLA_0B03650(TBLA0B03650)
TDL: TDEL_0A06520(TDEL0A06520)
KAF: KAFR_0A04000(KAFR0A04000)
PIC: PICST_33883(HNT3)
CAL: CAALFM_CR03440WA(CaO19.2376) CAALFM_CR03590CA(CaO19.4383)
SLB: AWJ20_1739(HNT3)
NCR: NCU06572
NTE: NEUTE1DRAFT136002(NEUTE1DRAFT_136002)
MGR: MGG_07058
SSCK: SPSK_09595
MAW: MAC_00501
MAJ: MAA_07304
BFU: BCIN_08g06920(Bchnt3)
MBE: MBM_01023
ANI: AN2139.2
ANG: ANI_1_1686134(An15g06480)
ABE: ARB_03228
TVE: TRV_02456
PTE: PTT_12333
CNE: CNC04800
CNB: CNBC2380
TASA: A1Q1_02217
ABP: AGABI1DRAFT122637(AGABI1DRAFT_122637)
ABV: AGABI2DRAFT187227(AGABI2DRAFT_187227)
MGL: MGL_0877
MRT: MRET_2631
DFA: DFA_01715
SPAR: SPRG_06657
 » show all
Reference
1  [PMID:16964241]
  Authors
Ahel I, Rass U, El-Khamisy SF, Katyal S, Clements PM, McKinnon PJ, Caldecott KW, West SC
  Title
The neurodegenerative disease protein aprataxin resolves abortive DNA ligation intermediates.
  Journal
Nature 443:713-6 (2006)
DOI:10.1038/nature05164
  Sequence
[gga:395173]
Reference
2  [PMID:24362567]
  Authors
Tumbale P, Williams JS, Schellenberg MJ, Kunkel TA, Williams RS
  Title
Aprataxin resolves adenylated RNA-DNA junctions to maintain genome integrity.
  Journal
Nature 506:111-5 (2014)
DOI:10.1038/nature12824
  Sequence
[hsa:54840]
Other DBs
ExplorEnz - The Enzyme Database: 3.6.1.71
IUBMB Enzyme Nomenclature: 3.6.1.71
ExPASy - ENZYME nomenclature database: 3.6.1.71
BRENDA, the Enzyme Database: 3.6.1.71

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