Hydrolases;
Acting on acid anhydrides;
In phosphorus-containing anhydrides
BRITE hierarchy

adenosine-5'-diphospho-5'-[DNA] hydrolase (adenosine 5'-phosphate-forming)

(1) adenosine-5'-diphospho-5'-[DNA] + H2O = AMP + phospho-5'-[DNA];
(2) adenosine-5'-diphospho-5'-(ribonucleotide)-[DNA] + H2O = AMP + 5'-phospho-(ribonucleotide)-[DNA]

adenosine-5'-diphospho-5'-[DNA];
H2O [CPD:C00001];
adenosine-5'-diphospho-5'-(ribonucleotide)-[DNA]

Aprataxin is a DNA-binding protein involved in different types of DNA break repair. The enzyme acts (among other activities) on abortive DNA ligation intermediates that contain an adenylate covalently linked to the 5'-phosphate DNA terminus. It also acts when the adenylate is covalently linked to the 5'-phosphate of a ribonucleotide linked to a DNA strand, which is the result of abortive ligase activty on products of EC 3.1.26.4, ribonuclease H, an enzyme that cleaves RNA-DNA hybrids on the 5' side of the ribonucleotide found in the 5'-RNA-DNA-3' junction. Aprataxin binds the adenylate group to a histidine residue within the active site, followed by its hydrolysis from the nucleic acid and eventual release, leaving a 5'-phosphate terminus that can be efficiently rejoined. The enzyme also possesses the activities of EC 3.6.1.70, guanosine-5'-diphospho-5'-[DNA] diphosphatase, and EC 3.6.1.72, DNA-3'-diphospho-5'-guanosine diphosphatase.

EC 3.6.1.71 created 2017 as EC 3.1.11.7, transferred 2019 to EC 3.6.1.71

1  [PMID:16964241]

Ahel I, Rass U, El-Khamisy SF, Katyal S, Clements PM, McKinnon PJ, Caldecott KW, West SC

The neurodegenerative disease protein aprataxin resolves abortive DNA ligation intermediates.

Nature 443:713-6 (2006)
DOI:10.1038/nature05164

[gga:395173]

2  [PMID:24362567]

Tumbale P, Williams JS, Schellenberg MJ, Kunkel TA, Williams RS

Aprataxin resolves adenylated RNA-DNA junctions to maintain genome integrity.

Nature 506:111-5 (2014)
DOI:10.1038/nature12824

[hsa:54840]