Entry
Name
DNA-3'-diphospho-5'-guanosine diphosphatase;
aprataxin;
DNA-3'pp5'G guanylate hydrolase;
APTX (gene name);
HNT3 (gene name)
Class
Hydrolases;
Acting on acid anhydrides;
In phosphorus-containing anhydrides
BRITE hierarchy
Sysname
[DNA]-3'-diphospho-5'-guanosine hydrolase (guanosine 5'-phosphate-forming)
Reaction(IUBMB)
[DNA]-3'-diphospho-5'-guanosine + H2O = [DNA]-3'-phosphate + GMP
Substrate
[DNA]-3'-diphospho-5'-guanosine;
H2O [CPD:
C00001 ]
Product
[DNA]-3'-phosphate;
GMP [CPD:
C00144 ]
Comment
Aprataxin is a DNA-binding protein that catalyses (among other activities) the 3' decapping of DNA-ppG (formed by EC
6.5.1.8 , 3'-phosphate/5'-hydroxy nucleic acid ligase) [1]. The enzyme binds the guanylate group to a histidine residue at its active site, forming a covalent enzyme-nucleotide phosphate intermediate, followed by the hydrolysis of the guanylate from the nucleic acid and its eventual release. The enzyme also possesses the activity of EC
3.6.1.71 , adenosine-5'-diphospho-5'-[DNA] diphosphatase, and EC
3.6.1.70 , guanosine-5'-diphospho-5'-[DNA] diphosphatase.
History
EC 3.6.1.72 created 2017 as EC 3.1.12.2, transferred 2019 to EC 3.6.1.72
Orthology
Genes
ANU : 117702473 117708186(Aptx)
PLOP : 125355383 125362898(Aptx)
TOD : 119230949(APTX) 119253332
PCW : 110204302 110220574(APTX)
GSTE : 104252030 104258213(APTX)
CCAR : 109051875 109051898 109065131
PFOR : 103137845 103137850
PLAI : 106943046 106943049
PMEI : 106911873 106919284
PMRN : 116937213 116950843(APTX)
DSI : Dsimw501_GD19289(Dsim_GD19289)
DYA : Dyak_GE25605 Dyak_GE27392 Dyak_GE28076 Dyak_GE29197
DGR : 116806024 116806025 116806026 116806027 116806028 116806306 122322685 122322686 122322689 6569964
DMO : Dmoj_GI16920 Dmoj_GI23101 Dmoj_GI23102
DAZ : 108608649 108614288 108614368
ACOZ : 120951911 120961674
AARA : 120897054 120901540
AMER : 121590176 121590404
ASTE : 118502892 118504672
AFUN : 125763320 125774808
AMOU : 128297066 128298194
CQU : CpipJ_CPIJ001673 CpipJ_CPIJ001675
CPII : 120418370 120418385
PFUC : 122514504 122520652
APLN : 108734079 108740333
CSAT : 104708982 104734171 104768332
PVY : 116115203 116138719 116138755
PCIN : 129289633 129297600
PALZ : 118037517 118045838
SSPL : 121795633 121800888
PSOM : 113291612 113310226 113322015
TAES : 123085582 123092448 123097769
PVIR : 120652712 120687912
SMO : SELMODRAFT_104482 SELMODRAFT_89021
CGR : 2890291(GVI51_K08789)
NCS : NCAS_0B01160(NCAS0B01160)
NDI : NDAI_0E01030(NDAI0E01030)
TBL : TBLA_0B03650(TBLA0B03650)
TDL : TDEL_0A06520(TDEL0A06520)
KAF : KAFR_0A04000(KAFR0A04000)
KNG : KNAG_0G02710(KNAG0G02710)
LBG : 92206012(LODBEIA_P08160)
CAL : CAALFM_CR03440WA(CaO19.2376) CAALFM_CR03590CA(CaO19.4383)
CDU : CD36_28600 CD36_28780
YLI : 2911961(YALI2_E01329g)
NTE : NEUTE1DRAFT136002(NEUTE1DRAFT_136002)
SMP : 10803913(SMAC4_04695)
TATV : 25779411(TrAtP1_011137)
TASP : 36616225(TrAFT101_008947)
PLJ : 28889959(PLICBS_008635)
CDET : 87941497(CDEST_04994)
BFU : BCIN_08g06920(Bchnt3)
PTRR : 6343811(PtrM4_109000)
CDEP : 91089741(L203_105532)
KMG : 30167525(I203_106436)
KNE : 92178259(IAR55_001000)
CCAC : CcaHIS019_0406190(HNT3)
PSQ : PUNSTDRAFT_114838 PUNSTDRAFT_28176
ABP : AGABI1DRAFT122637(AGABI1DRAFT_122637)
ABV : AGABI2DRAFT187227(AGABI2DRAFT_187227)
SCM : SCHCO_02605771(SCHCODRAFT_02605771)
TCR : 506989.50 510149.100
LMAT : 92514372(LSCM1_04351)
LOI : 92359440(LSCM4_03506)
LEIS : 94179103(GH5_03630)
LENR : 94171338(CUR178_04117)
LEIN : 94187411(JIQ42_03738)
PHET : 94286741(JKF63_00613)
EHX : EMIHUDRAFT_424881 EMIHUDRAFT_434812
» show all
Taxonomy
Reference
Authors
Das U, Chauleau M, Ordonez H, Shuman S
Title
Impact of DNA3'pp5'G capping on repair reactions at DNA 3' ends.
Journal
Reference
Authors
Chauleau M, Jacewicz A, Shuman S
Title
DNA3'pp5'G de-capping activity of aprataxin: effect of cap nucleoside analogs and structural basis for guanosine recognition.
Journal
Sequence
Other DBs
ExplorEnz - The Enzyme Database: 3.6.1.72
ExPASy - ENZYME nomenclature database: 3.6.1.72