KEGG ENZYME: 3.7.1.13

ENZYME: 3.7.1.13

Entry

EC 3.7.1.13                 Enzyme

Name

2-hydroxy-6-oxo-6-(2-aminophenyl)hexa-2,4-dienoate hydrolase;
CarC

Class

Hydrolases;
Acting on carbon-carbon bonds;
In ketonic substances

Sysname

(2E,4E)-6-(2-aminophenyl)-2-hydroxy-6-oxohexa-2,4-dienoate acylhydrolase

Reaction(IUBMB)

(2E,4E)-6-(2-aminophenyl)-2-hydroxy-6-oxohexa-2,4-dienoate + H2O = anthranilate + (2E)-2-hydroxypenta-2,4-dienoate

Reaction(KEGG)

(other) R05365

Substrate

(2E,4E)-6-(2-aminophenyl)-2-hydroxy-6-oxohexa-2,4-dienoate;
H2O [CPD:C00001]

Product

anthranilate [CPD:C00108];
(2E)-2-hydroxypenta-2,4-dienoate

Comment

This enzyme catalyses the third step in the aerobic degradation pathway of carbazole. The effect of the presence of an amino group or hydroxyl group at the 2-position of the substrate is small. The enzyme has no cofactor requirement [2].

History

EC 3.7.1.13 created 2010

Pathway

ec00621

Dioxin degradation

ec01100

Metabolic pathways

ec01120

Microbial metabolism in diverse environments

Orthology

K15756

2-hydroxy-6-oxo-6-(2'-aminophenyl)hexa-2,4-dienoate hydrolase

Genes

PSD:

DSC_14565

PRE:	PCA10_p0310(carC)

TTU:	TERTU_1260

CYQ:

Q91_0965

CZA:	CYCME_1631 CYCME_1639

CYY:	CPC19_07865 CPC19_07905

MGEO:

CFI10_14590

GBI:	PG2T_01235

RBU:	PG1C_11590

THAA:

CFI11_19415

SSAN:

NX02_18960

ERK:	CD351_08230

BTS:

Btus_2320

MFJ:	MFLOJ_23980

MGOR:

H0P51_02425

NARO:

CFH99_00800

» show all

Reference

1 [PMID:12619671]

Authors

Nojiri H, Taira H, Iwata K, Morii K, Nam JW, Yoshida T, Habe H, Nakamura S, Shimizu K, Yamane H, Omori T

Title

Purification and characterization of meta-cleavage compound hydrolase from a carbazole degrader Pseudomonas resinovorans strain CA10.

Journal

Biosci Biotechnol Biochem 67:36-45 (2003)
DOI:10.1271/bbb.67.36

Sequence

[pre:PCA10_p0310]

Reference

2 [PMID:12651123]

Authors

Riddle RR, Gibbs PR, Willson RC, Benedik MJ

Title

Purification and properties of 2-hydroxy-6-oxo-6-(2'-aminophenyl)hexa-2,4-dienoic acid hydrolase involved in microbial degradation of carbazole.

Journal

Protein Expr Purif 28:182-9 (2003)
DOI:10.1016/S1046-5928(02)00676-9

Sequence

[pre:PCA10_p0310]

Other DBs

ExplorEnz - The Enzyme Database:

3.7.1.13

IUBMB Enzyme Nomenclature:

3.7.1.13

ExPASy - ENZYME nomenclature database:

3.7.1.13

BRENDA, the Enzyme Database:

3.7.1.13

DBGET integrated database retrieval system