KEGG   ENZYME: 3.7.1.13
Entry
EC 3.7.1.13                 Enzyme                                 
Name
2-hydroxy-6-oxo-6-(2-aminophenyl)hexa-2,4-dienoate hydrolase;
CarC
Class
Hydrolases;
Acting on carbon-carbon bonds;
In ketonic substances
Sysname
(2E,4E)-6-(2-aminophenyl)-2-hydroxy-6-oxohexa-2,4-dienoate acylhydrolase
Reaction(IUBMB)
(2E,4E)-6-(2-aminophenyl)-2-hydroxy-6-oxohexa-2,4-dienoate + H2O = anthranilate + (2E)-2-hydroxypenta-2,4-dienoate
Reaction(KEGG)
(other) R05365
Substrate
(2E,4E)-6-(2-aminophenyl)-2-hydroxy-6-oxohexa-2,4-dienoate;
H2O [CPD:C00001]
Product
anthranilate [CPD:C00108];
(2E)-2-hydroxypenta-2,4-dienoate
Comment
This enzyme catalyses the third step in the aerobic degradation pathway of carbazole. The effect of the presence of an amino group or hydroxyl group at the 2-position of the substrate is small. The enzyme has no cofactor requirement [2].
History
EC 3.7.1.13 created 2010
Pathway
ec00621  Dioxin degradation
ec01100  Metabolic pathways
ec01120  Microbial metabolism in diverse environments
Orthology
K15756  2-hydroxy-6-oxo-6-(2'-aminophenyl)hexa-2,4-dienoate hydrolase
Genes
PSDDSC_14565
PREPCA10_p0310(carC)
TTUTERTU_1260
CYQQ91_0965
CZACYCME_1631 CYCME_1639
CYYCPC19_07865 CPC19_07905
MGEOCFI10_14590
GBIPG2T_01235
RBUPG1C_11590
THAACFI11_19415
SSANNX02_18960
ERKCD351_08230
BTSBtus_2320
EFFskT53_01950
MFJMFLOJ_23980
MGORH0P51_02425
NAROCFH99_00800
 » show all
Reference
1  [PMID:12619671]
  Authors
Nojiri H, Taira H, Iwata K, Morii K, Nam JW, Yoshida T, Habe H, Nakamura S, Shimizu K, Yamane H, Omori T
  Title
Purification and characterization of meta-cleavage compound hydrolase from a carbazole degrader Pseudomonas resinovorans strain CA10.
  Journal
Biosci Biotechnol Biochem 67:36-45 (2003)
DOI:10.1271/bbb.67.36
  Sequence
Reference
2  [PMID:12651123]
  Authors
Riddle RR, Gibbs PR, Willson RC, Benedik MJ
  Title
Purification and properties of 2-hydroxy-6-oxo-6-(2'-aminophenyl)hexa-2,4-dienoic acid hydrolase involved in microbial degradation of carbazole.
  Journal
Protein Expr Purif 28:182-9 (2003)
DOI:10.1016/S1046-5928(02)00676-9
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 3.7.1.13
IUBMB Enzyme Nomenclature: 3.7.1.13
ExPASy - ENZYME nomenclature database: 3.7.1.13
BRENDA, the Enzyme Database: 3.7.1.13

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