Entry
Name
2-hydroxy-6-oxonona-2,4-dienedioate hydrolase;
mhpC (gene name)
Class
Hydrolases;
Acting on carbon-carbon bonds;
In ketonic substances
BRITE hierarchy
Sysname
(2Z,4E)-2-hydroxy-6-oxona-2,4-dienedioate succinylhydrolase
Reaction(IUBMB)
(1) (2Z,4E)-2-hydroxy-6-oxonona-2,4-diene-1,9-dioate + H2O = (2Z)-2-hydroxypenta-2,4-dienoate + succinate [RN:
R02603 ];
(2) (2Z,4E,7E)-2-hydroxy-6-oxonona-2,4,7-triene-1,9-dioate + H2O = (2Z)-2-hydroxypenta-2,4-dienoate + fumarate [RN:
R06789 ]
Reaction(KEGG)
Substrate
(2Z,4E)-2-hydroxy-6-oxonona-2,4-diene-1,9-dioate [CPD:
C04479 ];
H2O [CPD:
C00001 ];
(2Z,4E,7E)-2-hydroxy-6-oxonona-2,4,7-triene-1,9-dioate [CPD:
C12624 ]
Product
(2Z)-2-hydroxypenta-2,4-dienoate;
succinate [CPD:
C00042 ];
fumarate [CPD:
C00122 ]
Comment
This enzyme catalyses a step in a pathway of phenylpropanoid compounds degradation. The first step of the enzyme mechanism involves a reversible keto-enol tautomerization [4].
History
EC 3.7.1.14 created 2011, modified 2012
Pathway
ec01120 Microbial metabolism in diverse environments
Orthology
K05714 2-hydroxy-6-oxonona-2,4-dienedioate hydrolase
Genes
ECOC : C3026_01720 C3026_24880
ECOO : ECRM13514_0497(mhpC)
ECOH : ECRM13516_0329(mhpC)
ECW : EcE24377A_0373(mhpC)
EDJ : ECDH1ME8569_0336(mhpC)
ELP : P12B_c0309(mhpC) P12B_c0367(mhpC)
REE : electrica_02137(mhpC)
LRI : NCTC12151_03197(mhpC)
VIB : VspSTUT11_14710(mhpC)
MSHE : MAALD49_00920(mhpC) MAALD49_03500(mhpC)
CYY : CPC19_08880 CPC19_11465
GBI : PG2T_10820 PG2T_14400
AQL : BXU06_16245 BXU06_17085
RPJ : N234_23050 N234_34820
CCUP : BKK81_19915 BKK81_25410
CUP : BKK80_21895 BKK80_30990
BCEN : DM39_2004 DM39_4969(bphD)
BPSL : WS57_03010 WS57_12785 WS57_14640
BGE : BC1002_4106 BC1002_4718 BC1002_5802
PDIO : PDMSB3_0769.1(mhpC)
PPAI : E1956_20820 E1956_24310
PANN : PanNE5_01430(mhpC_1) PanNE5_18680(mhpC_2)
BOJ : CBF45_02870 CBF45_10065
ACHR : C2U31_19585 C2U31_25035
AALN : Q3V95_01630 Q3V95_07620
PIG : EGT29_07515 EGT29_10735
KGY : EHF36_03280 EHF36_10465
ACIP : CBP36_07010 CBP36_10190
ACIN : CBP34_06360 CBP34_10010
LIM : L103DPR2_02271(mhpC)
DIH : G7047_03370 G7047_06675
AZO : azo1957(mhpC) azo1976(ohpC)
THK : CCZ27_00530(bphD) CCZ27_14715 CCZ27_16010
BCAN : BcanWSM471_04100 BcanWSM471_18230
SPHT : K426_04865 K426_12680 K426_15850 K426_18715
NCB : C0V82_22375 C0V82_22380
AGE : AA314_01990 AA314_05449
SPON : HME9304_02295(dmpD)
CGLE : NCTC11432_03205(hsaD_2)
CTAI : NCTC12078_02044(hsaD)
CTAK : 4412677_01675(mhpC_2)
CSAL : NBC122_00990(mhpC_1)
CJG : NCTC13459_01982(mhpC)
CANT : NCTC13489_02242(mhpC_1)
BKO : CKF48_07570 CKF48_08575
NDT : L1999_03365 L1999_23260
MAVI : RC58_15470 RC58_15715
MAVU : RE97_15500 RE97_15745
MCHI : AN480_04820 AN480_05125
MMAL : CKJ54_03670 CKJ54_03965
MMAN : MMAN_50340 MMAN_50940
MSER : MTY59_26620 MTY59_27220
MSA : Mycsm_04477 Mycsm_04570
MMI : MMAR_4727 MMAR_4778(bphD_1)
MMAE : MMARE11_37630 MMARE11_45550 MMARE11_46070(bphD_1)
MLI : MULP_04949 MULP_05008(bphD_1)
MPSE : MPSD_48940 MPSD_49550
MMC : Mmcs_1679 Mmcs_3688 Mmcs_3787 Mmcs_5440
MKM : Mkms_1708 Mkms_3761 Mkms_3860 Mkms_5609 Mkms_5837
MJL : Mjls_1652 Mjls_3701 Mjls_3791
MKN : MKAN_09895 MKAN_10225
MYV : G155_08405 G155_20880
MYE : AB431_00635 AB431_15420 AB431_21465 AB431_21710
MDX : BTO20_10350 BTO20_10645
MSHG : MSG_04265 MSG_04316
MFJ : MFLOJ_42990 MFLOJ_43620
MSTO : MSTO_47100 MSTO_47670
MSIM : MSIM_31510 MSIM_32110
MSAK : MSAS_26070 MSAS_26550 MSAS_43680(mhpC_2)
MKU : I2456_22530 I2456_22810
MLW : MJO58_23595 MJO58_23870
MXE : MYXE_09990 MYXE_10990
MNV : MNVI_09560 MNVI_10160
MPAG : C0J29_25985 C0J29_26280
MNM : MNVM_25910 MNVM_26190
MGOR : H0P51_24245 H0P51_24520
MCOO : MCOO_24090 MCOO_24680
MBAI : MB901379_04135(bphD_3) MB901379_04188(hsaD_2)
MSEO : MSEO_31690 MSEO_32210
MHEK : JMUB5695_00955 JMUB5695_01012
MGAU : MGALJ_00460(mhpC) MGALJ_26350
MLJ : MLAC_02350 MLAC_02980
MBRD : MBRA_23050 MBRA_23800
MMAM : K3U93_03580 K3U93_21220
MOT : LTS72_01380 LTS72_01645
MPAA : MKK62_03590 MKK62_03865
MKY : IWGMT90018_02310 IWGMT90018_54260 IWGMT90018_54960
MWU : PT015_14005 PT015_14330
MVA : Mvan_0538 Mvan_4167 Mvan_4246 Mvan_4414
MGI : Mflv_0545 Mflv_0627 Mflv_2400 Mflv_2487
MSP : Mspyr1_01980 Mspyr1_18320 Mspyr1_19140
MCB : Mycch_3603 Mycch_3690
MFT : XA26_16820 XA26_42590
MPHL : MPHLCCUG_03730(mhpC_1) MPHLCCUG_03812(hsaD_4)
MRH : MycrhN_3194 MycrhN_3917
MTHN : 4412656_03106(hsaD_2)
MHAS : MHAS_02555(mhpC) MHAS_02633(hsaD_3)
MAUU : NCTC10437_03908(mhpC_3) NCTC10437_03990(hsaD_2)
MMAG : MMAD_05810 MMAD_39150 MMAD_39670
MMOR : MMOR_05150 MMOR_23030
MFX : MFAL_01000 MFAL_01400
MAIC : MAIC_37930 MAIC_38430
MIJ : MINS_35310 MINS_35700
MALV : MALV_51940 MALV_53090
MPSC : MPSYJ_25740 MPSYJ_26380
MARZ : MARA_57610 MARA_58230
MGAD : MGAD_31800 MGAD_56240
MHEV : MHEL_05000 MHEL_05460
MSAR : MSAR_30930 MSAR_31320
MPOF : MPOR_42890 MPOR_43490
MMAT : MMAGJ_01350 MMAGJ_45290
MSEI : MSEDJ_50450 MSEDJ_51190
MFLV : NCTC10271_01471(hsaD_2)
MMON : EWR22_08545 EWR22_19475
MHOL : K3U96_07970 K3U96_08590 K3U96_21710
MPAE : K0O64_21000 K0O64_21470 K0O64_27370
MRF : MJO55_18850 MJO55_19220
MCRO : MI149_21575 MI149_21815
MGRO : FZ046_24235 FZ046_24700
MAUS : JN090_20315 JN090_20720
MFRE : EXE63_06725 EXE63_15710 EXE63_21965 EXE63_24125
MPAK : MIU77_10775 MIU77_10925
MJD : JDM601_3459(bphD_1) JDM601_3489
MTER : 4434518_03465(mhpC_1)
MHIB : MHIB_28550 MHIB_28810
MHER : K3U94_19605 K3U94_19735
MVM : MJO54_19510 MJO54_19645
NFR : ERS450000_00299(bphD_1) ERS450000_00748(bphD_2)
NWL : NWFMUON74_27310 NWFMUON74_27390
NGP : LTT66_14835 LTT66_34690 LTT66_34730
REB : XU06_01770 XU06_01800
RQI : C1M55_01860 C1M55_01890 C1M55_30820
ROP : ROP_46390(cmtE) ROP_pROB02-01910
ROA : Pd630_LPD12040 Pd630_LPD12045
RPY : Y013_12690 Y013_22375
RHQ : IM25_23230 IM25_24420 IM25_24490
RHOD : AOT96_12325 AOT96_12355 AOT96_33585 AOT96_34430
RRT : 4535765_01482(mhpC) 4535765_04583(hsaD_5)
RGO : KYT97_21265 KYT97_21295
ROZ : CBI38_30665 CBI38_32795
RHOJ : JVH1_35290 JVH1_41820
RHOH : CPI83_27220 CPI83_27250
PSOW : ABI214_15970 ABI214_20520
SKY : D0C37_00615 D0C37_00630 D0C37_31240
SGM : GCM10017557_70870(mhpC)
SVD : CP969_02210 CP969_02225 CP969_28365 CP969_28420
SPRA : CP972_28845 CP972_28900
SLON : LGI35_42220 LGI35_42225
MCAW : F6J84_03020 F6J84_14950
SALC : C2138_12160 C2138_12250
SALD : FVA74_01630 FVA74_01640
NMAR : HPC71_01545 HPC71_13465
NFC : KG111_01250 KG111_05705 KG111_16990
AEZ : C3E78_16575 C3E78_16580
ACTW : F7P10_19810 F7P10_19815
AGRA : AGRA3207_003620 AGRA3207_005067 AGRA3207_005068
AMAZ : LUW76_45515 LUW76_45915
FRE : Franean1_2894 Franean1_3934 Franean1_4748
FRI : FraEuI1c_1421 FraEuI1c_4146 FraEuI1c_5356
AMQ : AMETH_1183 AMETH_1857(mhpC)
AMOG : QRX60_28980 QRX60_32850 QRX60_49295
PAUT : Pdca_37920 Pdca_66970
PBRO : HOP40_05270 HOP40_17925 HOP40_22520 HOP40_33095 HOP40_33365
PPEL : H6H00_18715 H6H00_24665 H6H00_25970
LED : BBK82_32640 BBK82_43670
PMAD : BAY61_11500 BAY61_20695
MCRA : ID554_06940 ID554_07055
PSUU : Psuf_001740 Psuf_010720 Psuf_074270
BALA : DSM104299_04003(hsaD) DSM104299_05456(xylF_5)
SBAE : DSM104329_01247(todF_1)
NGA : Ngar_c32780 Ngar_c35080
» show all
Taxonomy
Reference
Authors
Burlingame R, Chapman PJ.
Title
Catabolism of phenylpropionic acid and its 3-hydroxy derivative by Escherichia coli.
Journal
Reference
Authors
Burlingame RP, Wyman L, Chapman PJ
Title
Isolation and characterization of Escherichia coli mutants defective for phenylpropionate degradation.
Journal
Reference
3
Authors
Lam W W Y, Bugg T D H.
Title
Chemistry of extradiol aromatic ring cleavage: isolation of a stable dienol ring fission intermediate and stereochemistry of its enzymatic hydrolytic clevage.
Journal
J Chem Soc, Chem Commun 10:1163-1164 (1994)
Reference
Authors
Lam WW, Bugg TD
Title
Purification, characterization, and stereochemical analysis of a C-C hydrolase: 2-hydroxy-6-keto-nona-2,4-diene-1,9-dioic acid 5,6-hydrolase.
Journal
Sequence
Reference
Authors
Ferrandez A, Garcia JL, Diaz E.
Title
Genetic characterization and expression in heterologous hosts of the 3-(3-hydroxyphenyl)propionate catabolic pathway of Escherichia coli K-12.
Journal
Sequence
Reference
Authors
Diaz E, Ferrandez A, Garcia JL.
Title
Characterization of the hca cluster encoding the dioxygenolytic pathway for initial catabolism of 3-phenylpropionic acid in Escherichia coli K-12.
Journal
Other DBs
ExplorEnz - The Enzyme Database: 3.7.1.14
ExPASy - ENZYME nomenclature database: 3.7.1.14
UM-BBD (Biocatalysis/Biodegradation Database): 3.7.1.14