Entry |
|
Name |
2-hydroxy-6-oxonona-2,4-dienedioate hydrolase;
mhpC (gene name)
|
Class |
Hydrolases;
Acting on carbon-carbon bonds;
In ketonic substances
|
Sysname |
(2Z,4E)-2-hydroxy-6-oxona-2,4-dienedioate succinylhydrolase
|
Reaction(IUBMB) |
(1) (2Z,4E)-2-hydroxy-6-oxonona-2,4-diene-1,9-dioate + H2O = (2Z)-2-hydroxypenta-2,4-dienoate + succinate [RN: R02603];
(2) (2Z,4E,7E)-2-hydroxy-6-oxonona-2,4,7-triene-1,9-dioate + H2O = (2Z)-2-hydroxypenta-2,4-dienoate + fumarate [RN: R06789]
|
Reaction(KEGG) |
|
Substrate |
(2Z,4E)-2-hydroxy-6-oxonona-2,4-diene-1,9-dioate [CPD: C04479];
H2O [CPD: C00001];
(2Z,4E,7E)-2-hydroxy-6-oxonona-2,4,7-triene-1,9-dioate [CPD: C12624]
|
Product |
(2Z)-2-hydroxypenta-2,4-dienoate;
succinate [CPD: C00042];
fumarate [CPD: C00122]
|
Comment |
This enzyme catalyses a step in a pathway of phenylpropanoid compounds degradation. The first step of the enzyme mechanism involves a reversible keto-enol tautomerization [4].
|
History |
EC 3.7.1.14 created 2011, modified 2012
|
Pathway |
ec01120 | Microbial metabolism in diverse environments |
|
Orthology |
K05714 | 2-hydroxy-6-oxonona-2,4-dienedioate hydrolase |
|
Genes |
» show all
|
Reference |
|
Authors |
Burlingame R, Chapman PJ. |
Title |
Catabolism of phenylpropionic acid and its 3-hydroxy derivative by Escherichia coli. |
Journal |
|
Reference |
|
Authors |
Burlingame RP, Wyman L, Chapman PJ |
Title |
Isolation and characterization of Escherichia coli mutants defective for phenylpropionate degradation. |
Journal |
|
Reference |
3 |
Authors |
Lam, W. W. Y and Bugg, T. D. H. |
Title |
Chemistry of extradiol aromatic ring cleavage: isolation of a stable dienol ring fission intermediate and stereochemistry of its enzymatic hydrolytic clevage. |
Journal |
J Chem Soc, Chem Commun 10:1163-1164 (1994) |
Reference |
|
Authors |
Lam WW, Bugg TD |
Title |
Purification, characterization, and stereochemical analysis of a C-C hydrolase: 2-hydroxy-6-keto-nona-2,4-diene-1,9-dioic acid 5,6-hydrolase. |
Journal |
|
Sequence |
|
Reference |
|
Authors |
Ferrandez A, Garcia JL, Diaz E. |
Title |
Genetic characterization and expression in heterologous hosts of the 3-(3-hydroxyphenyl)propionate catabolic pathway of Escherichia coli K-12. |
Journal |
|
Sequence |
|
Reference |
|
Authors |
Diaz E, Ferrandez A, Garcia JL. |
Title |
Characterization of the hca cluster encoding the dioxygenolytic pathway for initial catabolism of 3-phenylpropionic acid in Escherichia coli K-12. |
Journal |
|
Other DBs |
ExplorEnz - The Enzyme Database: | 3.7.1.14 |
ExPASy - ENZYME nomenclature database: | 3.7.1.14 |
UM-BBD (Biocatalysis/Biodegradation Database): | 3.7.1.14 |
|