KEGG   ENZYME: 3.7.1.17
Entry
EC 3.7.1.17                 Enzyme                                 
Name
4,5:9,10-diseco-3-hydroxy-5,9,17-trioxoandrosta-1(10),2-diene-4-oate hydrolase;
tesD (gene name);
hsaD (gene name)
Class
Hydrolases;
Acting on carbon-carbon bonds;
In ketonic substances
Sysname
4,5:9,10-diseco-3-hydroxy-5,9,17-trioxoandrosta-1(10),2-diene-4-oate hydrolase ( (2Z,4Z)-2-hydroxyhexa-2,4-dienoate-forming)
Reaction(IUBMB)
(1E,2Z)-3-hydroxy-5,9,17-trioxo-4,5:9,10-disecoandrosta-1(10),2-dien-4-oate + H2O = 3-[(3aS,4S,7aS)-7a-methyl-1,5-dioxo-octahydro-1H-inden-4-yl]propanoate + (2Z,4Z)-2-hydroxyhexa-2,4-dienoate [RN:R09883]
Reaction(KEGG)
R09883
Substrate
(1E,2Z)-3-hydroxy-5,9,17-trioxo-4,5:9,10-disecoandrosta-1(10),2-dien-4-oate [CPD:C04844];
H2O [CPD:C00001]
Product
3-[(3aS,4S,7aS)-7a-methyl-1,5-dioxo-octahydro-1H-inden-4-yl]propanoate [CPD:C20199];
(2Z,4Z)-2-hydroxyhexa-2,4-dienoate [CPD:C20240]
Comment
The enzyme is involved in the bacterial degradation of the steroid ring structure, and is involved in degradation of multiple steroids, such as testosterone [1], cholesterol [2], and sitosterol.
History
EC 3.7.1.17 created 2012
Pathway
ec00984  Steroid degradation
Orthology
K16050  4,5:9,10-diseco-3-hydroxy-5,9,17-trioxoandrosta-1(10),2-diene-4-oate hydrolase
Genes
RGLCS053_01755
VAFD1115_22495
PREPCA10_13810
PFUWKF707C_12160
PPUTL483_14175
PPUNPP4_27040(mhpC)
PVRPverR02_11710
PPUUPputUW4_01659(mhpC)
PKCPKB_4240
PFZAV641_10660
PLQAA042_19905
PPSYAOC04_07965
PSETTHL1_1432
PSILPMA3_11770
PKEDLD99_14450 DLD99_14755
PUMHGP31_20725
PPSHG5J76_13915
PLALFXN65_06415
PSEPC4K39_5448
PMUYKSS95_17290
PAZEKSS91_19300
PPHABVH74_17745
PTAENCTC10697_02081(mhpC)
PCAVD3880_08220 D3880_17375
ASEII8T81_09205
ACUMC9E88_011925
ADSFPL17_05420
ATNFM020_06325
SPLSpea_2147
SHLShal_2118
COLWA3Q33_18715
CBERB5D82_11110
PHAPSHAa0893(tesD)
PTNPTRA_a1026(hsaD)
PNGPNIG_a1077(hsaD)
SNANI6N98_01455 I6N98_10805
ZALAZF00_11435
HJABST95_09500
HALCEY643_13300
AFUSEYZ66_12960
KAKKalk_07850
MSECLN244_05065
SOKD0B54_04210 D0B54_06205
SINIGT972_14675
FCEJN531_005825
GBIPG2T_02180
GPBHDN1F_27100(tesD)
RINACS15_4349
REHH16_B0600(bphD)
CNCCNE_2c05450(fabG2)
CUHBJN34_30955
REUReut_A1513
CTIRALTA_B0404(mhpC)
CBWRR42_s2308
COXE0W60_08040
BHGI6G56_07560
BURBcep18194_B1463
BCJBCAM1609
BCENDM39_3387
BCEOI35_5469
BCTGEM_4115
BCEDDM42_6349
BCEPAPZ15_31175
BPYRABD05_27840
BCONNL30_04160
BDFWI26_21125
BTEIWS51_05675
BPSLWS57_06065
BMECWJ16_19990 WJ16_26770
BSTLBBJ41_21320 BBJ41_34095
BANNJFN94_25630
BULBW21_2676(hsaD)
BPLAbpln_1g12680
BUDAQ610_12660
BURKDM992_32645
BPHBphy_3515
BFNOI25_4585
PVEUC34_07060
PFIBPI93_021410
CABASBC2_49060(mhpC)
CTTCtCNB1_1354
CTESO987_07745
DIHG7047_09710
AZDCDA09_09460
THUAC731_008590
NTDEGO55_04995 EGO55_13685
CAULKCG34_11045
CONTQ29_16150
PALXGQA70_21455
NARSaro_3690
NPPPP1Y_Mpl3096
NOVTQ38_027285
NOTC7W88_20930
SWISwit_1754 Swit_1827 Swit_3338 Swit_4183
SPHDHY78_08860 HY78_16445 HY78_16815
RDICMV14_03930 CMV14_16820
SSANNX02_12715
SPANAWL63_19985
SPKCKC8_19645
SPHAD3Y57_15210
SLANGV829_01865
SPHBEP837_03392(hsaD)
PHAOHF685_05525
PHZCHX26_05310
SEDDERJ70_09520
AACOK1I37_05675
MTURv3569c(hsaD)
MTVRVBD_3569c
MTCMT3674(bphD)
MRAMRA_3608(bphD)
MTFTBFG_13602
MTBTBMG_03608
MTKTBSG_03635
MTZTBXG_003583
MTGMRGA327_22035
MTIMRGA423_22555
MTECCDC5079_3310
MTURCFBS_3786(bphD)
MTLCCDC5180_3262
MTOMTCTRI2_3634(bphD)
MTDUDA_3569c(bphD)
MTNERDMAN_3916(bphD)
MTJJ112_19220
MTUBMT7199_3632
MTUCJ113_24995
MTUEJ114_19090
MTXM943_18355
MTUHI917_25075
MTULTBHG_03510
MTUTHKBT1_3771(bphD)
MTUUHKBT2_3780(bphD)
MTQHKBS1_3783(bphD)
MBOBQ2027_MB3600C(hsad)
MBBBCG_3634c(bphD)
MBTJTY_3635(bphD)
MBMBCGMEX_3632c(bphD)
MBKK60_037070
MBXBCGT_3436
MAFMAF_35820(bphD)
MMICRN08_3946
MCEMCAN_35821(bphD)
MCQBN44_110064(bphD)
MCVBN43_90070(bphD)
MCXBN42_90067(bphD)
MCZBN45_100083(bphD)
MPAMAP_0498
MAOMAP4_3369
MAVIRC58_16710
MAVURE97_16745
MAVMAV_0592 MAV_2517
MITOCO_05010
MIAOCU_05050
MIDMIP_00941
MYOOEM_05070
MCHIAN480_02500
MIROCQ_05160
MMALCKJ54_02430
MLPMLM_0736
MMANMMAN_47950(bphD)
MSAMycsm_01915 Mycsm_01992 Mycsm_05839
MULMUL_4140(bphD)
MMCMmcs_4708
MKMMkms_4794
MJLMjls_5093
MMIMMAR_1502 MMAR_5064(bphD)
MMAEMMARE11_14020 MMARE11_48790(bphD)
MMMW7S_02445
MLIMULP_01620 MULP_05322(bphD)
MKNMKAN_12125 MKAN_22320
MYVG155_26480
MYEAB431_09620
MGOAFA91_10585
MHADB586_04450
MDXBTO20_04380 BTO20_24345
MSHGMSG_03543 MSG_04562(hsaD)
MFJMFLOJ_40660(hsaD)
MSTOMSTO_44610(hsaD) MSTO_55690
MSIMMSIM_29100(hsaD)
MSAKMSAS_13430 MSAS_29160(hsaD)
MKUI2456_25680
MGROFZ046_02945
MXEMYXE_05070(bphD)
MNVMNVI_12520(bphD)
MPAGC0J29_01145 C0J29_27830
MGORH0P51_01140 H0P51_25870
MCOOMCOO_27320(bphD) MCOO_46610(mhpC_3)
MBAIMB901379_04453(hsaD_3)
MSEOMSEO_27510 MSEO_34560(bphD)
MPSEMPSD_43530 MPSD_52650(hsaD)
MSHOMSHO_05480(bphD) MSHO_21750
MHEKJMUB5695_00468(bphD)
MGAUMGALJ_07320 MGALJ_38570
MLJMLAC_47230(bphD)
MBRDMBRA_01310 MBRA_20000(hsaD)
MSHJMSHI_38530(hsaD)
MDFK0O62_25970
MMAMK3U93_22690
MHOLK3U96_03075
MHERK3U94_06260
MSENK3U95_25515
MPAEK0O64_09000
MSPGF6B93_20490
MOTLTS72_27915
MVMMJO54_06430
MRFMJO55_02070 MJO55_24180
MPAAMKK62_02390 MKK62_17500
MCROMI149_09950
MSMMSMEG_6037
MSGMSMEI_5876(bphD)
MSBLJ00_29845
MSNLI99_29850
MSHLI98_29855
MVAMvan_0592 Mvan_5307
MGIMflv_1464
MSPMspyr1_47030
MCBMycch_4674
MNED174_24705
MYNMyAD_24240
MFTXA26_21680 XA26_53620
MPHLMPHLCCUG_00492(hsaD_1)
MVQMYVA_5217
MLLB1R94_19690 B1R94_25465
MRHMycrhN_2244
MTHN4412656_04093(hsaD_3)
MHASMHAS_03778(hsaD_5)
MDUMDUV_49000
MCHTMCHIJ_19300
MAUUNCTC10437_05068(hsaD_3)
MMAGMMAD_48580
MMORMMOR_11750
MFXMFAL_03550(mhpC) MFAL_32590
MAICMAIC_16360
MIJMINS_00700
MALVMALV_42130
MTYMTOK_21990
MPSCMPSYJ_15760
MARZMARA_47430
MGADMGAD_37690
MHEVMHEL_27640 MHEL_40900
MSARMSAR_10980
MANYMANY_02510(mhpC)
MAUBMAUB_47460(hsaD)
MPOFMPOR_54000
MPHUMPHO_21610 MPHO_43370
MMUCC1S78_007415 C1S78_025615
MMATMMAGJ_33770
MBOKMBOE_04970 MBOE_53390
MFGK6L26_05340
MSEIMSEDJ_02520
MFLVNCTC10271_00466(hsaD_1)
MCEEMCEL_37730
MIZBAB75_13835
MJDJDM601_1132
MMINMMIN_05410(mhpC_1)
ASDAS9A_0925(mhpC)
COKCOCCU_00295(hsaD1)
NFANFA_4730
NFRERS450000_03763(hsaD_1)
NCYNOCYR_0502
NBRO3I_002670
NNONONO_c05020(hsaD) NONO_c29060 NONO_c37010
NSLBOX37_02180
NSRNS506_01261(hsaD) NS506_06808
NTPCRH09_03310
NOZDMB37_23595
NODFOH10_28740
NAHF5544_02635
NADNCTC11293_01246(hsaD_2) NCTC11293_04256(hsaD_4)
NWLNWFMUON74_04940
NIEKV110_03030
NTCKHQ06_35200
NHUH0264_03855
NYALTV02_28885 LTV02_38600
NGPLTT66_00665 LTT66_14745 LTT66_15425
RHARHA1_ro04540(hsaD)
RERRER_07560
REYO5Y_03675
REBXU06_03915
RQIC1M55_04155
ROPROP_44550
ROAPd630_LPD00992
REQREQ_01450 REQ_06810 REQ_35100
RPYY013_19030
RBYCEJ39_05395
RAVAAT18_03970 AAT18_26195
RHWBFN03_15195
RRZCS378_19610
RHQIM25_08880
RHODAOT96_14550
RRT4535765_00583(hsaD_1) 4535765_04568(hsaD_4)
RCRNCTC10994_02137(hsaD_2)
RTMG4H71_07390
RKOJWS14_24490
RGOKYT97_23240
ROZCBI38_04175
GAMGII34_11030 GII34_18525
GPDGII33_04205 GII33_08475 GII33_16055
SPINKV203_17345 KV203_17540
TPRTpau_3844
TSMASU32_13590 ASU32_21900
TPULTPB0596_41850
DTMBJL86_2839 BJL86_3316
DITC3V38_06170
DIZCT688_16945
DPCA6048_17575
DLUA6035_16880
TOYFO059_01795
SMALSMALA_0968 SMALA_1212
SSOII1A49_08330 I1A49_09545
SDVBN159_8214
SALUDC74_7446
SALLSAZ_38435
SVTSVTN_35910
SLCSL103_24910
STSIA4E84_36410
SGMGCM10017557_80450(mhpC)
SAUOBV401_08635 BV401_09835
SALFSMD44_02218(hsaD)
SLXSLAV_27435(hsaD)
SAQUEJC51_46900
SPHVF9278_45575
SCHACP983_10625
SGXH4W23_38545
SSPBCP982_11900
SNFJYK04_02662(hsaD)
SVDCP969_28370
SPRACP972_28850
SXNIAG42_26650
SLFJEQ17_44910
SDECL3078_04210 L3078_31100
YIALO772_04905
MIOAOA12_18570
SALDFVA74_01355
ASUFMNQ99_15000
JTEASJ30_14570
NCANoca_2775
NDKI601_3469(hsaD_2)
NBEBack2_27210(mhpC_1)
NANOG5V58_09765
NAROCFH99_13525
NPIG7071_04055 G7071_15940
NPCKUV85_04285
PSIMKR76_14475
AEBC6I20_03265
FRIFraEuI1c_4100
AMDAMED_2523(mhpC) AMED_7185
AMNRAM_12825 RAM_36905
AMMAMES_2495(mhpC) AMES_7076
AMZB737_2496(mhpC) B737_7076
AOIAORI_2485(mhpC)
AJAAJAP_26400(hsaD)
AMQAMETH_4837(mhpC)
AMYCCU254_10465
AMYBBKN51_05025
AABA4R43_16040
AMYYYIM_14455(hsaD1)
AORISD37_13490
AACDLWP59_08655 LWP59_29195
PBROHOP40_22535
KALKALB_5123
KPHYAOZ06_42785
LEDBBK82_38920
ALOCRK59593
PMADBAY61_10960
SACEGIY23_13335
STPStrop_2670
SAQSare_2878
MCRAID554_07075
AMSAMIS_19190
AFSAFR_13045
AIHAiant_18880(mhpC)
VERHUT12_12175
SAWLNGM29_18535
 » show all
Reference
1  [PMID:16151114]
  Authors
Horinouchi M, Hayashi T, Koshino H, Kurita T, Kudo T
  Title
Identification of 9,17-dioxo-1,2,3,4,10,19-hexanorandrostan-5-oic acid, 4-hydroxy-2-oxohexanoic acid, and 2-hydroxyhexa-2,4-dienoic acid and related enzymes involved in testosterone degradation in Comamonas testosteroni TA441.
  Journal
Appl Environ Microbiol 71:5275-81 (2005)
DOI:10.1128/AEM.71.9.5275-5281.2005
Reference
2  [PMID:17264217]
  Authors
Van der Geize R, Yam K, Heuser T, Wilbrink MH, Hara H, Anderton MC, Sim E, Dijkhuizen L, Davies JE, Mohn WW, Eltis LD
  Title
A gene cluster encoding cholesterol catabolism in a soil actinomycete provides insight into Mycobacterium tuberculosis survival in macrophages.
  Journal
Proc Natl Acad Sci U S A 104:1947-52 (2007)
DOI:10.1073/pnas.0605728104
  Sequence
[mtu:Rv3569c]
Reference
3  [PMID:18097091]
  Authors
Lack N, Lowe ED, Liu J, Eltis LD, Noble ME, Sim E, Westwood IM
  Title
Structure of HsaD, a steroid-degrading hydrolase, from Mycobacterium tuberculosis.
  Journal
Acta Crystallogr Sect F Struct Biol Cryst Commun 64:2-7 (2008)
DOI:10.1107/S1744309107065931
  Sequence
[mtu:Rv3569c]
Reference
4  [PMID:19875455]
  Authors
Lack NA, Yam KC, Lowe ED, Horsman GP, Owen RL, Sim E, Eltis LD
  Title
Characterization of a carbon-carbon hydrolase from Mycobacterium tuberculosis involved in cholesterol metabolism.
  Journal
J Biol Chem 285:434-43 (2010)
DOI:10.1074/jbc.M109.058081
  Sequence
[mtu:Rv3569c]
Other DBs
ExplorEnz - The Enzyme Database: 3.7.1.17
IUBMB Enzyme Nomenclature: 3.7.1.17
ExPASy - ENZYME nomenclature database: 3.7.1.17
UM-BBD (Biocatalysis/Biodegradation Database): 3.7.1.17
BRENDA, the Enzyme Database: 3.7.1.17

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