KEGG   ENZYME: 3.7.1.4
Entry
EC 3.7.1.4                  Enzyme                                 
Name
phloretin hydrolase;
ErPhy;
lactase-phlorerin hydrolase;
C-acylphenol hydrolase;
2',4,4',6'-tetrahydroxydehydrochalcone 1,3,5-trihydroxybenzenehydrolase (incorrect)
Class
Hydrolases;
Acting on carbon-carbon bonds;
In ketonic substances
Sysname
phloretin acylhydrolase (phloroglucinol-forming)
Reaction(IUBMB)
phloretin + H2O = phloretate + phloroglucinol [RN:R02901]
Reaction(KEGG)
R02901;
(other) R13082
Substrate
phloretin [CPD:C00774];
H2O [CPD:C00001]
Product
phloretate [CPD:C01744];
phloroglucinol [CPD:C02183]
Comment
Also hydrolyses other C-acylated phenols related to phloretin. Isolated from the fungus Aspergillus niger and the bacteria Pantoea agglomerans and Eubacterium ramulus.
History
EC 3.7.1.4 created 1972, modified 2018
Orthology
K22906  phloretin hydrolase
Genes
RGERGE_01480 RGE_12780
TAKTharo_0198
LPGBB562_02250
CLJCLJU_c30240
CLSCXIVA_00870 CXIVA_01400 CXIVA_03650 CXIVA_10480 CXIVA_16390 CXIVA_20190
CSRCspa_c22710
CAHCAETHG_1031
CSQCSCA_1095
CBUTATN24_18505
CRWCROST_033500(phy_1)
CFBCLFE_033220(phy)
CAUNCLAUR_043040(phy)
CALEFDN13_06055
FPLAA4U99_15225
ALKAJ0B03_05305
ETMCE91St48_18380 CE91St48_29990
AFNAcfer_0965
 » show all
Reference
1  [PMID:5354935]
  Authors
Chatterjee AK, Gibbins LN
  Title
Metabolism of phloridzin by Erwinia herbicola: nature of the degradation products, and the purification and properties of phloretin hydrolase.
  Journal
J Bacteriol 100:594-600 (1969)
DOI:10.1128/JB.100.2.594-600.1969
Reference
2  [PMID:5441377]
  Authors
Minamikawa T, Jayasankar NP, Bohm BA, Taylor IE, Towers GH.
  Title
An inducible hydrolase from Aspergillus niger, acting on carbon-carbon bonds, for phlorrhizin and other C-acylated phenols.
  Journal
Biochem J 116:889-97 (1970)
DOI:10.1042/bj1160889
Reference
3  [PMID:15466559]
  Authors
Schoefer L, Braune A, Blaut M
  Title
Cloning and expression of a phloretin hydrolase gene from Eubacterium ramulus and characterization of the recombinant enzyme.
  Journal
Appl Environ Microbiol 70:6131-7 (2004)
DOI:10.1128/AEM.70.10.6131-6137.2004
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 3.7.1.4
IUBMB Enzyme Nomenclature: 3.7.1.4
ExPASy - ENZYME nomenclature database: 3.7.1.4
BRENDA, the Enzyme Database: 3.7.1.4
CAS: 37289-38-6

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