KEGG   ENZYME: 4.1.1.119
Entry
EC 4.1.1.119                Enzyme                                 

Name
phenylacetate decarboxylase;
phdB (gene name)
Class
Lyases;
Carbon-carbon lyases;
Carboxy-lyases
Sysname
phenylacetate carboxy-lyase
Reaction(IUBMB)
phenylacetate = toluene + CO2 [RN:R12504]
Reaction(KEGG)
R12504
Substrate
phenylacetate [CPD:C07086]
Product
toluene [CPD:C01455];
CO2 [CPD:C00011]
Comment
This bacterial enzyme, isolated from anoxic, toluene-producing microbial communities, is a glycyl radical enzyme. It needs to be activated by a dedicated activating enzyme (PhdA). The activase catalyses the reductive cleavage of AdoMet, producing a 5'-deoxyadenosyl radical that leads to the production of the glycyl radical in PhdB.
History
EC 4.1.1.119 created 2019
Reference
1  [PMID:27506494]
  Authors
Zargar K, Saville R, Phelan RM, Tringe SG, Petzold CJ, Keasling JD, Beller HR
  Title
In vitro Characterization of Phenylacetate Decarboxylase, a Novel Enzyme Catalyzing Toluene Biosynthesis in an Anaerobic Microbial Community.
  Journal
Sci Rep 6:31362 (2016)
DOI:10.1038/srep31362
Reference
2  [PMID:29556105]
  Authors
Beller HR, Rodrigues AV, Zargar K, Wu YW, Saini AK, Saville RM, Pereira JH, Adams PD, Tringe SG, Petzold CJ, Keasling JD
  Title
Discovery of enzymes for toluene synthesis from anoxic microbial communities.
  Journal
Nat Chem Biol 14:451-457 (2018)
DOI:10.1038/s41589-018-0017-4
  Sequence
Reference
3  [PMID:31512343]
  Authors
Rodrigues AV, Tantillo DJ, Mukhopadhyay A, Keasling JD, Beller HR.
  Title
Insight into the Mechanism of Phenylacetate Decarboxylase (PhdB), a Toluene-Producing Glycyl Radical Enzyme.
  Journal
Chembiochem 21:663-671 (2020)
DOI:10.1002/cbic.201900560
Other DBs
ExplorEnz - The Enzyme Database: 4.1.1.119
IUBMB Enzyme Nomenclature: 4.1.1.119
ExPASy - ENZYME nomenclature database: 4.1.1.119
BRENDA, the Enzyme Database: 4.1.1.119

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